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CD155/PVR Antibody (D171)

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Western Blot: CD155/PVR Antibody (D171) [NB600-1241] - Analysis of 25 ug of mouse stomach (Lane 1), U-937 (Lane 2) and Hela (Lane 3) cell lysates.
Western Blot: CD155/PVR Antibody (D171) [NB600-1241] - Analysis of 25 ug of mouse stomach (Lane 1), U-937 (Lane 2) and Hela (Lane 3) cell lysates.
Immunocytochemistry/ Immunofluorescence: CD155/PVR Antibody (D171) [NB600-1241] - Analysis of CD155 (green) showing staining in the membrane of Hela cells (right) compared to a negative control without primary antibody ...read more
Flow Cytometry: CD155/PVR Antibody (D171) [NB600-1241] - Analysis of CD155 in Hela cells compared to an isotype control (blue).
Immunocytochemistry/ Immunofluorescence: CD155/PVR Antibody (D171) [NB600-1241] - Analysis of CD155 (green) showing staining in the membrane of U-87 MG cells (right) compared to a negative control without primary ...read more
Flow Cytometry: CD155/PVR Antibody (D171) [NB600-1241] - Analysis of U937 cells compared to an isotype control (blue).

Product Details

Summary
Reactivity Hu, Mu, PmSpecies Glossary
Applications WB, Flow, ICC/IF
Clone
D171
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

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CD155/PVR Antibody (D171) Summary

Additional Information
Clone D171 was used by HLDA to establish CD designation.
Immunogen
HeLa cells
Epitope
Amino acids 35 - 50.
Localization
Cell membrane.
Specificity
Polio Virus Receptor (D171)
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PVR
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry 2 ug
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:100
  • Western Blot 1:10 - 1:100
Application Notes
WB: not suitable. This antibody protects HeLa cells against thecytopathic effect of all three poliovirus serotypes. Intact Ig or Fab fragment of this antibody prevents binding of 35S-labeled poliovirus to HeLa cells.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4) and 0.2% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein G purified

Alternate Names for CD155/PVR Antibody (D171)

  • CD155 antigen
  • CD155
  • HVED
  • NECL5
  • Necl-5
  • nectin-like 5
  • Nectin-like protein 5
  • poliovirus receptor
  • PVR
  • PVS
  • PVSFLJ25946
  • Tage4

Background

Polio Virus Receptor (PVR) is a member of Ig-superfamily with 3 Ig-domains in arrangement V-C-C. Four mRNAs are produced from single gene for PVR, (mapped to human chromosome band 19q13.1-13.2). Two of the mRNAs (called H20A and H20B or alpha and delta) encode cell surface molecules of about 43kDa and 45kDa polypeptide backbone, respectively. Two other splice variants, beta and gamma encode secreted molecules of about 44kDa. The cytoplasmic tails are short (aa 35-50) and are rich in serine phosphorylated residues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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ICC/IF Video Protocol

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Bioinformatics

Gene Symbol PVR
Entrez