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Carbonic Anhydrase VIII/CA8 Antibody (308320) [Unconjugated]

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Western blot shows lysates of human brain (cerebellum) tissue and mouse brain (cerebellum) tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse Carbonic Anhydrase VIII/CA8 Monoclonal Antibody (Catalog # MAB2187) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Carbonic Anhydrase VIII/CA8 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of human brain (cerebellum) tissue and mouse brain (cerebellum) tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse Carbonic Anhydrase VIII/CA8 Monoclonal Antibody ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB
Clone
308320
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price
Carrier Free
MAB2187-SP
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Carbonic Anhydrase VIII/CA8 Antibody (308320) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Carbonic Anhydrase VIII/CA8
Ala2-Gln290
Accession # P35219
Specificity
Detects human Carbonic Anhydrase VIII/CA8 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human CA1, 2, 3, 4, 9, 10, 12, 13, or 14 is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CA8
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Carbonic Anhydrase VIII/CA8 Antibody (308320) [Unconjugated]

  • CA8
  • CALS
  • CALScarbonate dehydratase
  • CAMRQ3
  • Carbonic Anhydrase VIII
  • carbonic anhydrase VIIIMGC99509
  • carbonic anhydrase-like sequence
  • carbonic anhydrase-related protein
  • CARP
  • CARPCA-related protein
  • CA-VIII
  • MGC120502

Background

Carbonic Anhydrase VIII (CA8), also called CA‑related protein (CARP), is a cytosolic protein without CA activity (i.e., the reversible hydration of CO2) due to point mutations in the zinc-binding site (1, 2). Nevertheless, it has esterase activity. CA8 is expressed exclusively in Purkinje cells of the cerebellum, where it binds inositol 1,4,5-triphosphate receptor type 1 (3). CA8 overexpression in human colorectal cancer and non-small cell lung cancer indicates that it plays a role in the process of invasion in these types of malignancy (4, 5). The 289 amino acid rhCA8 has a predicted molecular mass of approximately 33 kDa. It shares 98% amino acid identity with mouse, rat and bovine CA8 and 78% with Xenopus.

  1. Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
  2. Sjoblom, J. et al. (1996) FEBS Lett. 398: 322.
  3. Hirota, J. et al. (2003) Biochem. J. 372: 435.
  4. Miyaji, E. et al. (2003) J. Pathol. 201: 37.
  5. Lu, S.H. et al. (2004) Lung Cancer 44: 273.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CA8
Uniprot