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Carbonic Anhydrase IX/CA9 Antibody (303123) [Alexa Fluor® 488]

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U-87 MG human glioblastoma/astrocytoma cell line was stained with Mouse Anti-Human Carbonic Anhydrase IX/CA9 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB2188G, filled histogram) or isotype control antibody (Catalog # IC003G, open histogram). View our protocol for Staining Membrane-associated Proteins.
U-87 MG human glioblastoma/astrocytoma cell line was stained with Mouse Anti-Human Carbonic Anhydrase IX/CA9 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB2188G, filled histogram) or isotype control ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
303123
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 488
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB2188U-100UG
Alexa Fluor 405
FAB2188V-100UG
Alexa Fluor 532
FAB2188X
Alexa Fluor 594
FAB2188T-100UG
Alexa Fluor 647
FAB2188R-100UG
Alexa Fluor 700
FAB2188N-100UG
Alexa Fluor 750
FAB2188S-100UG
Allophycocyanin
FAB2188A
Allophycocyanin/Cy7
FAB2188APCCY7
Biotin
FAB2188B
CoraFluor 1
FAB2188CL1
DyLight 350
FAB2188D
DyLight 405
FAB2188E
DyLight 488
FAB2188K
DyLight 550
FAB2188L
DyLight 594
FAB2188M
DyLight 650
FAB2188W
DyLight 680
FAB2188Y
DyLight 755
FAB2188Z
Fluorescein
FAB2188F
HRP
FAB2188H
Janelia Fluor 525
FAB2188JF525
Janelia Fluor 549
FAB2188I
Janelia Fluor 646
FAB2188J
Janelia Fluor 669
FAB2188JF669
PE/Atto594
FAB2188PEATT594
PE/Cy5.5
FAB2188PECY55
PE/Cy7
FAB2188PECY7
PerCP
FAB2188C
Phycoerythrin
FAB2188P
Unconjugated
MAB2188-100
mFluor Violet 450 SE
FAB2188MFV450
mFluor Violet 500 SE
FAB2188MFV500
mFluor Violet 610 SE
FAB2188MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB2188-100
Carrier Free
FAB2188F
Carrier Free
FAB2188P
Carrier Free
FAB2188A
Carrier Free
MAB2188-SP
Carrier Free
MAB2188-500
Carrier Free
MAB2188-100
Carrier Free
FAB2188V-100UG
Carrier Free
FAB2188U-100UG
Carrier Free
FAB2188T-100UG
Carrier Free
FAB2188S-100UG
Carrier Free
FAB2188R-100UG
Carrier Free
FAB2188N-100UG
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Carbonic Anhydrase IX/CA9 Antibody (303123) [Alexa Fluor® 488] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Carbonic Anhydrase IX
Pro59-Asp414
Accession # Q16790
Specificity
Detects human Carbonic Anhydrase IX (CA9) in direct ELISAs. In direct ELISAs, this antibody does not cross-react with recombinant mouse (rm) CA9 or with rhCA1, 2, 3, 4, 5A, 6, 7, 8, 10, 12, 13, or 14.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
CA9
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25-1 ug/10^6 cells
Application Notes
Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells.
Publications
Read Publications using
FAB2188G in the following applications:

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Reconstitution Instructions


Notes


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Carbonic Anhydrase IX/CA9 Antibody (303123) [Alexa Fluor® 488]

  • CA9
  • CAIX
  • CA-IX
  • Carbonate dehydratase IX
  • carbonic anhydrase 9
  • Carbonic Anhydrase IX
  • carbonic dehydratase
  • EC 4.2.1.1
  • G250
  • Membrane antigen MN
  • MN
  • P54/58N
  • PMW1
  • RCC
  • RCC-associated antigen G250
  • RCC-associated protein G250
  • Renal cell carcinoma-associated antigen G250

Background

Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1-3). Topics in the CA meeting (6th International Conference on the CAs, June 20-25, 2003; Slovakia) ranged from use of CAs as markers for tumor and hypoxia in clinic, as nutritional supplement in milk, and as a tool for CO2 removal and mosquito control in industry. CA9, also known as membrane antigen MN and renal cell carcinoma (RCC)-associated antigen G250, is a transmembrane enzyme expressed primarily in carcinoma cells. It is one of the best markers for hypoxia and for RCC (4, 5). rhCA9 corresponds to the extracellular portion of human CA9.

  1. Pastorek, J. et al. (1994) Oncogene 9:2877.
  2. Opavsky, R. et al. (1996) Genomics 33:480.
  3. Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
  4. Kaluzova, M. et al. (2004) Mol. Cell Biol. 24:5757.
  5. Mukouyama, H. et al. (2004) Clin. Cancer Res. 10:1421.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAB2188G
Carbonic Anhydrase IX/CA9 Antibody (303123) [Alexa Fluor® 488]
Species: Hu
Applications: Flow

Publications for Carbonic Anhydrase IX/CA9 Antibody (FAB2188G)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Flow Cytometry.


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(2)
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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CA9
Uniprot