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Mouse Calcineurin ELISA Kit (Colorimetric)

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA
Suitable Sample Type
Tissue homogenates, Cell lysates, Cell culture supernates and other biological fluids
Standard Curve Range
0.156 - 10 ng/mL
Sensitivity
0.060 ng/mL

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Mouse Calcineurin ELISA Kit (Colorimetric) Summary

Description
Assay time: 3 hours
Standard Curve Range
0.156 - 10 ng/mL
Sensitivity
0.060 ng/mL
Assay Type
Sandwich ELISA
Inter-Assay
%CV < 12
Intra-Assay
%CV < 10
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
PPP3CA

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Assay Diluent A
  2. Assay Diluent B
  3. Detection Reagent A
  4. Detection Reagent B
  5. Instruction manual
  6. Plate sealer for 96 wells
  7. Pre-coated ready to use 96-well strip plate
  8. Standard Diluent
  9. Standard
  10. Stop Solution
  11. TMB Substrate
  12. Wash Buffer (30 x concentrate)

Alternate Names for Mouse Calcineurin ELISA Kit (Colorimetric)

  • calcineurin A alpha
  • calcineurin subunit B type 1
  • Calcineurin
  • CALN
  • CALNA
  • CALNB1
  • CCN1
  • CNA1
  • CNB1

Background

Enables several functions, including ATPase binding activity; calmodulin binding activity; and calmodulin-dependent protein phosphatase activity. Involved in several processes, including calcineurin-NFAT signaling cascade; peptidyl-serine dephosphorylation; and response to calcium ion. Located in several cellular components, including cytosol; dendritic spine; and nucleoplasm. Part of calcineurin complex. Colocalizes with cytoplasmic side of plasma membrane. Implicated in developmental and epileptic encephalopathy 91. Biomarker of focal segmental glomerulosclerosis and schizophrenia. [provided by Alliance of Genome Resources, Apr 2022]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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GAPDH: More than a housekeeping gene
GAPDH is a 146kD tetramer glycolytic pathway metabolic enzyme composed of four 30-40 kDa subunits. It is responsible for reversibly phosphorylating its substrate glyceraldehyde 3-phosphate within the glycolytic pathway.  Apart from its role in gl...  Read full blog post.

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Bioinformatics

Gene Symbol PPP3CA