Novus Biologicals products are now on bio-techne.com

Calcineurin A Antibody (2G8)

Images

 
Western Blot: Calcineurin A Antibody (2G8) [H00005530-M03] - Analysis of PPP3CA expression in transfected 293T cell line by PPP3CA monoclonal antibody (M03), clone 2G8. Lane 1: PPP3CA transfected lysate (Predicted MW: ...read more
Immunocytochemistry/ Immunofluorescence: Calcineurin A Antibody (2G8) [H00005530-M03] - Analysis of monoclonal antibody to PPP3CA on HeLa cell. Antibody concentration 10 ug/ml
Immunohistochemistry-Paraffin: Calcineurin A Antibody (2G8) [H00005530-M03] - Analysis of monoclonal antibody to PPP3CA on formalin-fixed paraffin-embedded human stomach. Antibody concentration 1.5 ug/ml
Western Blot: Calcineurin A Antibody (2G8) [H00005530-M03] - Analysis of PPP3CA expression in rat brain.

Product Details

Summary
Reactivity Hu, Rt, PoSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clone
2G8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Calcineurin A Antibody (2G8) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
PPP3CA (NP_000935, 1 a.a. ~ 84 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSEPKAIDPKLSTTDRVVKAVPFPPSHRLTAKEVFDNDGKPRVDILKAHLMKEGRLEESVALRIITEGASILRQEKNLLDIDAP
Specificity
PPP3CA (2G8)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
PPP3CA
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control. It has been used for IHC-P.
Publications
Read Publications using H00005530-M03.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Calcineurin A Antibody (2G8)

  • calcineurin A alpha
  • Calcineurin A
  • Calmodulin-dependent calcineurin A subunit alpha isoform
  • CALN
  • CALNA
  • CALNAcatalytic subunit, alpha isoform
  • CAM-PRP catalytic subunit
  • CNA
  • CNA1
  • CNA1CALNA1
  • EC 3.1.3.16
  • PP2B
  • PP3CA
  • PPP2B
  • PPP2BCCN1
  • PPP3CA
  • protein phosphatase 2B, catalytic subunit, alpha isoform
  • protein phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform(calcineurin A alpha)
  • protein phosphatase 3, catalytic subunit, alpha isozyme
  • serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform

Background

Calcineurin, a major soluble calmodulin binding protein in the brain, a Ca2+/calmodulin dependent serine/threonine protein phosphatase, with a relatively narrow substrate specificity. This metalloenzyme, also known as phosphatase 2B, is a heterodimer composed of a calmodulin binding, catalytic alpha subunit 61 kDa, calcineurin A) and calcium binding beta subunit (18 kDa, calcineurin B). The Ca2+ binding subunit, calcineurin B, is immunologically conserved from yeast to mammals. The presence of 2 different calcineurin B isoforms (beta 1 and beta 2) has been reported in rat testis. The catalytic subunit of calcineurin, calcineurin A, isolated from different tissues or different organisms, exhibits some immunological heterogeneity. Further, there are at least 2 isoforms of calcineurin A in bovine brain (alpha 1 and alpha 2, 61 and 59 kDa, respectively).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF4055
Species: Mu
Applications: IHC, Simple Western, WB
AF1640
Species: Hu
Applications: ELISA(Cap), ELISA(Det), ELISA(Sta), IHC, WB
AF1348
Species: Hu, Mu, Rt
Applications: Simple Western, WB
NBP2-14871
Species: Mu, Rt
Applications: ICC/IF, WB
NB120-2860
Species: Ba, Bv, Ch, Hu, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
NBP2-15669
Species: Mu, Rt, Ze
Applications: IHC-WhMt, IHC, WB
NBP3-32848
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NBP2-93612
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-84037
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
AF4309
Species: Hu, Mu
Applications: ICC, IHC, WB
AF1680
Species: Mu
Applications: IHC, WB
DVE00
Species: Hu
Applications: ELISA
NBP2-50037
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, KO, WB
NBP2-33903
Species: Hu
Applications: IHC, IHC-P
202-IL
Species: Hu
Applications: BA
MAB8930
Species: Hu
Applications: ICC, Simple Western, WB
AF887
Species: Hu, Mu, Rt
Applications: CyTOF-ready, IHC, ICFlow, Simple Western, WB
H00005530-M03
Species: Hu, Rt, Po
Applications: WB, ELISA, ICC/IF, IHC

Publications for Calcineurin A Antibody (H00005530-M03)(2)


Showing Publications 1 - 2 of 2.
Publications using H00005530-M03 Applications Species
Totzeck A, Boengler K, van de Sand A et al. No impact of protein phosphatases on connexin 43 phosphorylation in ischemic preconditioning. Am J Physiol Heart Circ Physiol. 2008-10-03 [PMID: 18835920]
Mardjiati S, Wulan M, Laswati H, Hadi U, et al. Physical Exercise in Clinical Stage IIhuman Immunodeficiency Virus Infection Patients’ Increasesskeletal Muscle MAss Through the Increasing of Myogenic Regulatory Factors Expression Indian Journal of Forensic Medicine & Toxicology 2021-09-08

Reviews for Calcineurin A Antibody (H00005530-M03) (0)

There are no reviews for Calcineurin A Antibody (H00005530-M03). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Calcineurin A Antibody (H00005530-M03) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Calcineurin A Products

Research Areas for Calcineurin A Antibody (H00005530-M03)

Find related products by research area.

Blogs on Calcineurin A.

The role of HIF-1 Alpha signaling in the retina under hypoxic conditions
Hypoxia inducible factor 1 (HIF-1) is a protein that plays an essential role in hypoxia, or low levels of cellular oxygen. HIF-1 is a heterodimeric protein that consists of a constitutively expressed beta subunit and oxygen related alpha subunit.  ...  Read full blog post.

Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Calcineurin A Antibody (2G8) and receive a gift card or discount.

Bioinformatics

Gene Symbol PPP3CA
Entrez
OMIM
Uniprot