We are setting up an ELISA type of assay for CA125. On your web page: Applications: Uses: Detection of CA125 antigen. Suggested pairs for sandwich immunoassay (capture-detection): NB120-10032 (capture) with NBP2-11624 or NB600-1468 as detection. Can you send me more details how these antibodies have been working in your sandwich immunoassay?

Name: CA125/MUC16 Antibody (X75)
Brand: Novus Biologicals
SKU: NB600-1468
Price: 319 USD

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Accepted Answer

These antibodies have been tested with very general sandwich ELISA, without any assay optimization, just to determine which MAbs work together as pairs. Coat plate with NB120-10032 in alkaline carbonate buffer Post-coat with casein Add HRP-conjugated NBP2-11624 and antigen to plate Incubate microplates for 45 min at room temperature with shaking Wash 5 times with saline-Tween Add 100 ul of substrate Stop enzymatic reaction addition of 5% sulphuric acid. Measure the optical density. The sensitivity without optimization has been around 5 IU/ml. But we really do not have any recommended protocol and the suitability and sensitivity of the Abs should be determined in your own assay.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	HuSpecies Glossary
Applications	WB, ELISA
Clone	X75
Clonality	Monoclonal
Host	Mouse
Conjugate	Unconjugated

Immunogen	Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with CA125 antigen purified from human ovarian carcinoma (MW > 1 MDa).
Epitope	B1 epitope group
Localization	Cell Membrane
Specificity	CA125 antigen, epitope specificity group B (ISOBM classification) similar to M-11.
Isotype	IgG1
Clonality	Monoclonal
Host	Mouse
Gene	MUC16
Purity	Ion exchange chromatography
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Store at 4C. Do not freeze.
Buffer	10mM Tris (pH 7.5) and 0.15M NaCl
Preservative	0.05% Sodium Azide
Purity	Ion exchange chromatography

Publications using NB600-1468	Applications	Species
Chongsaritsinsuk J, Steigmeyer AD, Mahoney KE et al. Glycoproteomic landscape and structural dynamics of TIM family immune checkpoints enabled by mucinase SmE Nature communications 2023-10-04 [PMID: 37794035] (WB, Human) Details: 1:1000 WB dilution	WB	Human
K Pedram, NN Laqtom, DJ Shon, A Di Spiezio, NM Riley, P Saftig, M Abu-Remail, CR Bertozzi Lysosomal cathepsin D mediates endogenous mucin glycodomain catabolism in mammals Proceedings of the National Academy of Sciences of the United States of America, 2022-09-19;119(39):e2117105119. 2022-09-19 [PMID: 36122205]
Hosu O, Ravalli A, Piccolo GM et al. SMARTPHONE-BASED IMMUNOSENSOR FOR CA125 DETECTION. Talanta. 2017-01-27 [PMID: 28213228]
McLemore MR, Miaskowski C, Lee K et al. Differences in the Molecular Species of CA125 Across the Phases of the Menstrual Cycle. Biol Res Nurs 2015-01-07 [PMID: 25566791]
Heinzelmann-Schwarz VA et al. Overexpression of the cell adhesion molecules DDR1, Claudin 3, Ep-CAM in metaplastic ovarian epithelium ovarian cancer. Clin Cancer Res 10:4427-36. 2004-01-01 [PMID: 15240533]

CA125/MUC16 Antibody (X75)

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Gene Symbol	MUC16
Entrez	94025(Human)
Uniprot	Q8WXI7()