![Western Blot: BRN3B/POU4F2 Antibody [NBP2-26061] - (1ug/ml) staining of Human Cerebellum (A) + peptide (B), Mouse Brain (C) + peptide (D), and Rat Brain (E) + peptide (F) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/nbp2-26061_goat-polyclonal-brn3b-pou4f2-antibody-16520239183328.jpg "Western Blot: BRN3B/POU4F2 Antibody [NBP2-26061] - (1ug/ml) staining of Human Cerebellum (A) + peptide (B), Mouse Brain (C) + peptide (D), and Rat Brain (E) + peptide (F) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.")
| Reactivity | Hu, Mu, Rt, Bv, CaSpecies Glossary |
| Applications | WB, ELISA |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Description | Please note, this antibody is considered to be "Innovators Grade". Innovators Grade antibodies have been validated for limited uses and require additional characterization for most new species/applications. These antibodies are directed against antigens that have limited or no commercial antibodies available for them, or for which there are limited expression data available to us. In an effort to encourage customer feedback, Novus has made these antibodies available through our Innovators Reward program. Under this program, Novus will provide you a 50% refund on the purchased product as well as a 50% discount on a future product of equal value in exchange for your new data. Please contact us at innovators@novusbio.com for more details. |
| Immunogen | Peptide with sequence HGLPSHMGCMSDVD corresponding to internal region according to NP_004566.2. |
| Specificity | This antibody is not expected to cross-react to the similar POU4F3 (GeneID 5459) |
| Predicted Species | Bovine (100%), Canine (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | POU4F2 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Approx. 35kDa band observed in Human, Mouse and Rat Brain lysates (calculated MW of 43.1kDa according to Human NP_004566.2). This band was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 1-3ug/ml. Primary incubation 1 hour at room temperature. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for BRN3B/POU4F2 Antibody (NBP2-26061)Find related products by research area.
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