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Human beta I Tubulin ELISA Kit (Colorimetric)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and other biological fluids
Standard Curve Range
1.56 - 100 ng/mL
Sensitivity
0.55 ng/mL

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Human beta I Tubulin ELISA Kit (Colorimetric) Summary

Description
Assay time: 3 hours
Standard Curve Range
1.56 - 100 ng/mL
Sensitivity
0.55 ng/mL
Assay Type
Sandwich ELISA
Inter-Assay
%CV < 12
Intra-Assay
%CV < 10
Spike Recovery
84 - 99%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
TUBB1

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Assay Diluent A
  2. Assay Diluent B
  3. Detection Reagent A
  4. Detection Reagent B
  5. Instruction manual
  6. Plate sealer for 96 wells
  7. Pre-coated ready to use 96-well strip plate
  8. Standard Diluent
  9. Standard
  10. Stop Solution
  11. TMB Substrate
  12. Wash Buffer (30 x concentrate)

Alternate Names for Human beta I Tubulin ELISA Kit (Colorimetric)

  • beta tubulin 1, class VI
  • betaI Tubulin
  • beta-I Tubulin
  • dJ543J19.4
  • TUBB1
  • tubulin beta-1 chain
  • tubulin, beta 1

Background

Microtubules are involved in a wide variety of cellular processes, including mitosis, morphogenesis, plateletformation, and mobility of cilia and flagella. Circulating platelets carry a single marginal microtubule coil that iswound in 8 to 12 turns and is responsible for platelet shape. TUBB1 is the major beta-tubulin expressed in plateletsand megakaryocytes and is required for optimal platelet assembly (Wang et al., 1986 (PubMed 3782288); Schulze et al.,2004 (PubMed 15315966)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol TUBB1