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beta I Tubulin Antibody (OTI4A3)

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Western Blot: beta I Tubulin Antibody (OTI4A3) [NBP2-46245] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY beta 1 Tubulin.
Western Blot: beta I Tubulin Antibody (OTI4A3) [NBP2-46245] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY beta 1 Tubulin.
Immunocytochemistry/ Immunofluorescence: beta I Tubulin Antibody (OTI4A3) [NBP2-46245] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY beta I Tubulin.
Immunohistochemistry: beta I Tubulin Antibody (OTI4A3) [NBP2-46245] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Western Blot: beta I Tubulin Antibody (OTI4A3) [NBP2-46245] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; ...read more
Immunohistochemistry: beta I Tubulin Antibody (OTI4A3) [NBP2-46245] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: beta I Tubulin Antibody (OTI4A3) [NBP2-46245] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: beta I Tubulin Antibody (OTI4A3) [NBP2-46245] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: beta I Tubulin Antibody (OTI4A3) [NBP2-46245] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, PmSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
OTI4A3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-72225AF350
Alexa Fluor 405
NBP2-72225AF405
Alexa Fluor 488
NBP2-72225AF488
Alexa Fluor 532
NBP2-72225AF532
Alexa Fluor 594
NBP2-72225AF594
Alexa Fluor 647
NBP2-72225AF647
Alexa Fluor 700
NBP2-72225AF700
Alexa Fluor 750
NBP2-72225AF750
Allophycocyanin
NBP2-72225APC
Biotin
NBP2-72225B
CoraFluor 1
NBP2-72225CL1
DyLight 350
NBP2-72225UV
DyLight 405
NBP2-72225V
DyLight 488
NBP2-72225G
DyLight 550
NBP2-72225R
DyLight 594
NBP2-72225DL594
DyLight 650
NBP2-72225C
DyLight 680
NBP2-72225FR
DyLight 755
NBP2-72225IR
FITC
NBP2-72225F
HRP
NBP2-72225H
Janelia Fluor 525
NBP2-72225JF525
Janelia Fluor 549
NBP2-72225JF549
Janelia Fluor 646
NBP2-72225JF646
Janelia Fluor 669
NBP2-72225JF669
PE
NBP2-72225PE
PerCP
NBP2-72225PCP
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-72225
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beta I Tubulin Antibody (OTI4A3) Summary

Immunogen
Full length human recombinant protein of human TUBB1(NP_110400) produced in HEK293T cell.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
TUBB1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:200-2000
Theoretical MW
50.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP2-46245 in the following applications:

  • WB
    1 publication

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for beta I Tubulin Antibody (OTI4A3)

  • beta tubulin 1, class VI
  • betaI Tubulin
  • beta-I Tubulin
  • dJ543J19.4
  • TUBB1
  • tubulin beta-1 chain
  • tubulin, beta 1

Background

Microtubules are involved in a wide variety of cellular processes, including mitosis, morphogenesis, plateletformation, and mobility of cilia and flagella. Circulating platelets carry a single marginal microtubule coil that iswound in 8 to 12 turns and is responsible for platelet shape. TUBB1 is the major beta-tubulin expressed in plateletsand megakaryocytes and is required for optimal platelet assembly (Wang et al., 1986 (PubMed 3782288); Schulze et al.,2004 (PubMed 15315966)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for beta I Tubulin Antibody (NBP2-46245)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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Bioinformatics

Gene Symbol TUBB1