Home » BCL-W/BCL2L2 » BCL-W Antibodies » BCL-W/BCL2L2 Antibody (90316) [Unconjugated]

BCL-W/BCL2L2 Antibody (90316) [Unconjugated]

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Western blot shows lysates of WS-1 human fetal skin fibroblast cell line and CTLL-2 mouse cytotoxic T cell line. The PVDF membrane was probed with 1 µg/mL Human Bcl-w Monoclonal Antibody (Catalog # MAB824) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, Recombinant Human Bcl-w Minus C-Terminus (Catalog # 824-BW) (1 ng) was included. A specific band for Bcl-w was detected at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 9with 25 mM Tris and 0.15 M NaCl.
Western blot shows lysates of WS-1 human fetal skin fibroblast cell line and CTLL-2 mouse cytotoxic T cell line. The PVDF membrane was probed with 1 µg/mL Human Bcl-w Monoclonal Antibody (Catalog # MAB824) followed by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clone
90316
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price
Carrier Free
MAB824-SP
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BCL-W/BCL2L2 Antibody (90316) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Bcl-w
Met1-Thr172
Accession # Q92843
Specificity
Detects human Bcl-w in Western blots.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
BCL2L2
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C, as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 6 months, -20 to -70 °C under sterile conditions after opening.
Buffer
Supplied as a 0.2 μm filtered solution in PBS with Sodium Azide. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
Sodium Azide
Concentration
LYOPH

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BCL-W/BCL2L2 Antibody (90316) [Unconjugated]

  • Apoptosis regulator Bcl-W
  • BCL2L2
  • Bcl2-L-2
  • BCL2-like 2
  • Bclw
  • Bcl-w
  • BCLWbcl-2-like protein 2
  • KIAA0271BCL2-L-2

Background

Bcl-w is a member of the Bcl-2 family of proteins that regulates outer mitochondrial membrane permeability (1, 2). Bcl-w is an anti-apoptotic member that prevents release of cytochrome c from the mitochondria intermembrane space into the cytosol. Bcl-w is required for normal sperm maturation (3, 4, 5). Natural Bcl-w contains a carboxyl-terminal mitochondria targeting sequence. Recombinant Bcl-w missing the mitochondrial targeting sequence maintains its ability to neutralize pro-apoptotic Bcl-2 family members. Neutralization by Bcl-w appears to be through binding the BH3 region of pro-apoptotic Bcl-2 family members. This activity does not require the mitochondrial targeting sequence.

 

  1. Gross, A. et al. (1999) Genes and Develop. 13:1899.
  2. Kroemer, G. (1997) Nature Med. 3:614.
  3. Ross, J.A., et al. (1998) Nat. Genet. 18:251.
  4. Print, C.G. et al. (1998) Proc. Natl. Acad. Sci. USA 95:12323.
  5. Yan, W. et al. (2000) Mol. Endocrin. 14:682.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol BCL2L2
Uniprot