Home » Bcl-2 related protein A1 » Bcl-2 related protein A1 ELISA Kits » Human Bcl-2 related protein A1 ELISA Kit (Colorimetric)
Novus Biologicals products are now on bio-techne.com

Human Bcl-2 related protein A1 ELISA Kit (Colorimetric)

 Submit a Review

Images

 
(
Enlarge)
ELISA: Human Bcl-2 related protein A1 ELISA Kit (Colorimetric) [NBP2-69948] - Samples were spiked with high concentrations of Human Bcl-2 related protein A1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Human Bcl-2 related protein A1 ELISA Kit (Colorimetric) [NBP2-69948] - Samples were spiked with high concentrations of Human Bcl-2 related protein A1 and diluted with Reference Standard & Sample Diluent to ...read more
ELISA: Human Bcl-2 related protein A1 ELISA Kit (Colorimetric) [NBP2-69948] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
31.25 - 2000 pg/mL
Sensitivity
18.75 pg/mL

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

Human Bcl-2 related protein A1 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Human BCL2A1 in samples. No significant cross-reactivity or interference between Human BCL2A1 and analogues was observed.
Standard Curve Range
31.25 - 2000 pg/mL
Sensitivity
18.75 pg/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 5.27%
Intra-Assay
CV% < 5.16%
Spike Recovery
85-106%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
BCL2A1

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Human Bcl-2 related protein A1 ELISA Kit (Colorimetric)

  • ACC-1
  • ACC-2
  • Bcl-2 related protein A1
  • BCL2A1
  • Bcl2L5
  • Bcl2-L-5
  • BCL2L5HBPA1
  • Bcl-2-like protein 5
  • BCL2-related protein A1
  • Bfl-1
  • BFL1bcl-2-related protein A1
  • GRS
  • GRSbcl2-L-5
  • hematopoietic BCL2-related protein A1
  • Hemopoietic-specific early response protein
  • Protein BFL-1
  • Protein GRS

Background

Bcl-2 related protein A1; also known as Bcl2A1 and Bfl1, is an anti-apoptotic Bcl-2 family member preferentially expressed in hematopoietic and endothelial cells (1). Bfl1 is involved in a variety of cellular activities such as embryonic development, homeostatis and tumorigenesis. Expression of Bfl1 is stimulated by NF-kB in response to inflammatory stimuli and is up-regulated by TNF-alpha, IL1-beta, IGF1, GMC-SF, CD40 and phorbol ester (2-3). Blf1 has also been shown to reduce the release of pro-apoptotic cytochrome c from the mitochondria, block caspase activation and suppress apoptosis induced by p53 (4-5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

PP2A alpha [p
Tyr307] Antibody [Unc ...
AF3989
Western Blot PP2A alpha [p Tyr307] Antibody [Unconjugated]
(12 Publications)
Species: Hu, Mu, Rt
Applications: WB
MAT1A Antibody
NBP3-13066
Immunohistochemistry-Paraffin MAT1A Antibody
Immunohistochemistry-Paraffin MAT1A Antibody
Western Blot MAT1A Antibody
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
RFC1 Antibody
[Unconjugated]
AF6457
Western Blot RFC1 Antibody [Unconjugated]
Species: Hu
Applications: WB
RFC2 Antibody
(OTI2B2)
NBP2-45570
Flow Cytometry RFC2 Antibody (OTI2B2)
Immunohistochemistry RFC2 Antibody (OTI2B2)
Immunohistochemistry RFC2 Antibody (OTI2B2)
Species: Hu, Mu, Rt
Applications: Flow, IHC, IHC-P, WB
RFC4 Antibody
(OTI1A8)
NBP2-45946
Immunocytochemistry/ Immunofluorescence RFC4 Antibody (OTI1A8)
Immunohistochemistry RFC4 Antibody (OTI1A8)
Immunohistochemistry RFC4 Antibody (OTI1A8)
Species: Ca, Hu, Pm, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
Bcl-xL Antibody
NB100-56104
Western Blot Bcl-xL Antibody
Immunohistochemistry-Paraffin Bcl-xL Antibody
Western Blot Bcl-xL Antibody
(1 Review)
(3 Publications)
Species: Ca, Hu, Mu, Rt
Applications: IHC, IHC-Fr, IHC-P, IP, WB
PCNA Antibody
(PC10)
NB500-106
Immunocytochemistry/ Immunofluorescence PCNA Antibody (PC10)
Western Blot PCNA Antibody (PC10)
Flow Cytometry PCNA Antibody (PC10)
(5 Reviews)
(46 Publications)
Species: Ch, Dr, Fi, Hu, Mar, Mu, Po, Pm, Rb, Rt, Ye, Ze
Applications: ChIP, ChIP, ELISA, Flow, ICC/IF, IHC-FrFl, IHC, IHC-Fr, IHC-P, IP, Simple Western, WB
ATP6V0A1 Antibody
NBP1-89342
Immunocytochemistry/ Immunofluorescence ATP6V0A1 Antibody
Immunohistochemistry-Paraffin ATP6V0A1 Antibody
Immunohistochemistry-Paraffin ATP6V0A1 Antibody
(1 Review)
(13 Publications)
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, IP, WB
Bcl-2 Antibody
NB100-56098
Western Blot Bcl-2 Antibody
Western Blot Bcl-2 Antibody
Western Blot Bcl-2 Antibody
(2 Reviews)
(26 Publications)
Species: Ca, Hu, Mu, Rt
Applications: IHC, IHC-P, IP, WB
TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(818 Publications)
Species: Hu
Applications: BA
NFkB p105/p50
Antibody (2J10D7) - B ...
NB100-56583
Flow Cytometry NFkB p105/p50 Antibody (2J10D7) - BSA Free
Flow Cytometry NFkB p105/p50 Antibody (2J10D7) - BSA Free
Flow Cytometry NFkB p105/p50 Antibody (2J10D7) - BSA Free
(1 Review)
(5 Publications)
Species: Hu, Rb, Rt
Applications: Flow, IHC, IHC-P, KO, WB
Reduced Folate
Carrier/SLC19A1 Antib
NBP1-59904
Immunohistochemistry-Paraffin Reduced Folate Carrier/SLC19A1 Antibody
Western Blot Reduced Folate Carrier/SLC19A1 Antibody
(1 Publication)
Species: Hu
Applications: IHC, IHC-P, WB
Caspase-3 Antibody
[Unconjugated] - Acti ...
AF835
Immunocytochemistry Caspase-3 Antibody [Unconjugated] - Active
Immunohistochemistry Caspase-3 Antibody [Unconjugated] - Active
Immunocytochemistry Caspase-3 Antibody [Unconjugated] - Active
(7 Reviews)
(389 Publications)
Species: Hu, Mu
Applications: ICC, IHC
Bax Antibody (6A7)
NBP1-28566
Western Blot Bax Antibody (6A7)
Western Blot Bax Antibody (6A7)
Immunohistochemistry-Frozen Bax Antibody (6A7)
(3 Reviews)
(21 Publications)
Species: Bv, Hu, Pm, Mu, Po, Rt
Applications: Func, IHC, IHC-Fr, IHC-P, WB
ERK2 Antibody
[Unconjugated]
AF1230
Western Blot ERK2 Antibody [Unconjugated]
Immunohistochemistry ERK2 Antibody [Unconjugated]
Knockout Validated ERK2 Antibody [Unconjugated]
(3 Publications)
Species: Hu, Mu, Rt
Applications: IHC, KO, WB
c-jun [p Ser63]
Antibody (1018Y) [Unc ...
MAB8930
Western Blot c-jun [p Ser63] Antibody (1018Y) [Unconjugated]
Immunocytochemistry c-jun [p Ser63] Antibody (1018Y) [Unconjugated]
Simple Western c-jun [p Ser63] Antibody (1018Y) [Unconjugated]
(1 Review)
(3 Publications)
Species: Hu
Applications: ICC, Simple Western, WB
Mcl-1 Antibody
NB100-56146
Western Blot Mcl-1 Antibody
Immunohistochemistry-Paraffin Mcl-1 Antibody
(1 Review)
(5 Publications)
Species: Hu
Applications: IHC, IHC-P, IP, WB
p21/CIP1/CDKN1A
Antibody (WA-1)
NBP2-29463
Immunohistochemistry-Paraffin p21/CIP1/CDKN1A Antibody (WA-1)
(24 Publications)
Species: Pm, Hu, Pm, Mu
Applications: Flow, ICC/IF, IHC, IHC-P, WB

Publications for Bcl-2 related protein A1 ELISA Kit (NBP2-69948) (0)

There are no publications for Bcl-2 related protein A1 ELISA Kit (NBP2-69948).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Bcl-2 related protein A1 ELISA Kit (NBP2-69948) (0)

There are no reviews for Bcl-2 related protein A1 ELISA Kit (NBP2-69948). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Bcl-2 related protein A1 ELISA Kit (NBP2-69948) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Bcl-2 related protein A1 Products

Research Areas for Bcl-2 related protein A1 ELISA Kit (NBP2-69948)

Find related products by research area.

Blogs on Bcl-2 related protein A1

There are no specific blogs for Bcl-2 related protein A1, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
TNF-alpha [Unconjugated]
210-TA-005
Caspase&#x2011;3 was detected in immersion fixed anti-FAS treated Jurkat human acute T cell leukemia cell line using 0.3 &micro;g/mL Human/Mouse Active Caspase&#x2011;3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF835) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Caspase-3 Antibody [Unconjugated] - Active
AF835

Contact Information

Product PDFs

  SDS
  Human Bcl-2 related protein A1 ELISA Kit (Colorimetric) Manual
  PDF Datasheet
  Request CofA

Review this Product

Be the first to review our Human Bcl-2 related protein A1 ELISA Kit (Colorimetric) and receive a gift card or discount.


Bioinformatics

Gene Symbol BCL2A1