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Bag-1 Antibody (2D3)

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Western Blot: Bag-1 Antibody (2D3) [H00000573-M02] - BAG1 monoclonal antibody (M02), clone 2D3. Analysis of BAG1 expression in NIH/3T3.
Western Blot: Bag-1 Antibody (2D3) [H00000573-M02] - BAG1 monoclonal antibody (M02), clone 2D3. Analysis of BAG1 expression in NIH/3T3.
Immunocytochemistry/ Immunofluorescence: Bag-1 Antibody (2D3) [H00000573-M02] - Analysis of monoclonal antibody to BAG1 on HeLa cell. Antibody concentration 35 ug/ml.
Immunohistochemistry-Paraffin: Bag-1 Antibody (2D3) [H00000573-M02] - Analysis of monoclonal antibody to BAG1 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 3 ug/ml.
Western Blot: Bag-1 Antibody (2D3) [H00000573-M02] - analysis of BAG1 expression in transfected 293T cell line by BAG1 monoclonal antibody (M02), clone 2D3. Lane 1: BAG1 transfected lysate (38.878 KDa). Lane 2: ...read more
Western Blot: Bag-1 Antibody (2D3) [H00000573-M02] - BAG1 monoclonal antibody (M02), clone 2D3 Analysis of BAG1 expression in HeLa.
Western Blot: Bag-1 Antibody (2D3) [H00000573-M02] - BAG1 monoclonal antibody (M02), clone 2D3. Analysis of BAG1 expression in PC-12.
Western Blot: Bag-1 Antibody (2D3) [H00000573-M02] - BAG1 monoclonal antibody (M02), clone 2D3. Analysis of BAG1 expression in LNCaP.
Western Blot: Bag-1 Antibody (2D3) [H00000573-M02] - BAG1 monoclonal antibody (M02), clone 2D3. Analysis of BAG1 expression in Raw 264.7.
Western Blot: Bag-1 Antibody (2D3) [H00000573-M02] - BAG1 monoclonal antibody (M02), clone 2D3. Analysis of BAG1 expression in Jurkat.
Sandwich ELISA: Bag-1 Antibody (2D3) [H00000573-M02] - Detection limit for recombinant GST tagged BAG1 is approximately 0.03ng/ml as a capture antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clone
2D3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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Bag-1 Antibody (2D3) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
BAG1 (NP_004314, 241 a.a. ~ 345 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EKIADQLEELNKELTGIQQGFLPKDLQAEALCKLDRRVKATIEQFMKILEEIDTLILPENFKDSRLKRKGLVKKVQAFLAECDTVEQNICQETERLQSTNFALAE
Specificity
BAG1 - BCL2-associated athanogene
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
BAG1
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot 1:500
Application Notes
Antibody reactivity against recominant protein, transfected lysate and cell lysate for WB. It has been used for IF, IHC-P and ELISA.
Publications
Read Publication using H00000573-M02.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Bag-1 Antibody (2D3)

  • BAG family molecular chaperone regulator 1
  • Bag1
  • Bag-1
  • Bcl-2 associating athanogene-1 protein
  • Bcl-2-associated athanogene 1
  • BCL2-associated athanogene
  • Bcl-2-binding protein
  • glucocortoid receptor-associated protein RAP46,46-KD
  • HAP
  • RAP46

Background

The oncogene BCL2 is a membrane protein that blocks a step in a pathway leading to apoptosis or programmed cell death. The protein encoded by this gene binds to BCL2 and is referred to as BCL2-associated athanogene. It enhances the anti-apoptotic effects of BCL2 and represents a link between growth factor receptors and anti-apoptotic mechanisms. At least three protein isoforms are encoded by this mRNA through the use of a non-AUG (CUG) start site, and alternative, downstream, AUG translation initiation sites.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Bag-1 Antibody (H00000573-M02)(1)


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Bioinformatics

Gene Symbol BAG1
Entrez
OMIM
Uniprot