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![Western Blot: Bag-1 Antibody [NB100-56082] - Analysis of BAG-1 in normal brain tissue lysates.](http://images.novusbio.com/fullsize/Bag-1-Antibody-Western-Blot-NB100-56082-img0009.jpg "Western Blot: Bag-1 Antibody [NB100-56082] - Analysis of BAG-1 in normal brain tissue lysates.")
![Immunohistochemistry-Paraffin: Bag-1 Antibody [NB100-56082] - Formalin-fixed, paraffin-embedded human breast tissue stained for BAG-1 expression using this antibody at 1:2000. A. normal breast epithelium. B. breast cancer. A1 and B1 are high magnifications from A and B, respectively. Hematoxylin-eosin counterstain.](http://images.novusbio.com/fullsize/Bag-1-Antibody-Immunohistochemistry-Paraffin-NB100-56082-img0008.jpg)
![Western Blot: Bag-1 Antibody [NB100-56082] - BAG-1 levels in human neuroblastoma. Image from verified customer review.](http://images.novusbio.com/fullsize/Bag-1-Antibody-Western-Blot-NB100-56082-img0010.jpg)
![Immunohistochemistry-Paraffin: Bag-1 Antibody [NB100-56082] - Formalin-fixed, paraffin-embedded mouse ischemic brain stained for BAG-1 expression using NB100-56082 at 1:2000. A1 is a higher magnification of A. Differential Bag-1 expression levels are seen in the hypothalmic nuclei. The variation in expression levels from lack of Bag-1 staining to strong staining may be linked to differential responses of the hypothalmic nuclei to ischemic stress. Hematoxylin-eosin counterstain.](http://images.novusbio.com/fullsize/Bag-1-Antibody-Immunohistochemistry-Paraffin-NB100-56082-img0007.jpg)
![Western Blot: Bag-1 Antibody (2D3) [H00000573-M02] - BAG1 monoclonal antibody (M02), clone 2D3. Analysis of BAG1 expression in NIH/3T3.](http://images.novusbio.com/fullsize/Bag-1-Antibody-2D3-Western-Blot-H00000573-M02-img0025.jpg "Western Blot: Bag-1 Antibody (2D3) [H00000573-M02] - BAG1 monoclonal antibody (M02), clone 2D3. Analysis of BAG1 expression in NIH/3T3.")
![Immunocytochemistry/Immunofluorescence: Bag-1 Antibody (2D3) [H00000573-M02] - Analysis of monoclonal antibody to BAG1 on HeLa cell. Antibody concentration 35 ug/ml.](http://images.novusbio.com/fullsize/Bag-1-Antibody-2D3-Immunocytochemistry-Immunofluorescence-H00000573-M02-img0016.jpg)
![Immunohistochemistry-Paraffin: Bag-1 Antibody (2D3) [H00000573-M02] - Analysis of monoclonal antibody to BAG1 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/Bag-1-Antibody-2D3-Immunohistochemistry-Paraffin-H00000573-M02-img0018.jpg)
![Western Blot: Bag-1 Antibody (2D3) [H00000573-M02] - analysis of BAG1 expression in transfected 293T cell line by BAG1 monoclonal antibody (M02), clone 2D3. Lane 1: BAG1 transfected lysate (38.878 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Bag-1-Antibody-2D3-Western-Blot-H00000573-M02-img0013.jpg)
![Western Blot: Bag-1 Antibody (2D3) [H00000573-M02] - BAG1 monoclonal antibody (M02), clone 2D3 Analysis of BAG1 expression in HeLa.](http://images.novusbio.com/fullsize/Bag-1-Antibody-2D3-Western-Blot-H00000573-M02-img0020.jpg)
![Western Blot: Bag-1 Antibody (2D3) [H00000573-M02] - BAG1 monoclonal antibody (M02), clone 2D3. Analysis of BAG1 expression in PC-12.](http://images.novusbio.com/fullsize/Bag-1-Antibody-2D3-Western-Blot-H00000573-M02-img0021.jpg)
![Western Blot: Bag-1 Antibody (2D3) [H00000573-M02] - BAG1 monoclonal antibody (M02), clone 2D3. Analysis of BAG1 expression in LNCaP.](http://images.novusbio.com/fullsize/Bag-1-Antibody-2D3-Western-Blot-H00000573-M02-img0022.jpg)
![Western Blot: Bag-1 Antibody (2D3) [H00000573-M02] - BAG1 monoclonal antibody (M02), clone 2D3. Analysis of BAG1 expression in Raw 264.7.](http://images.novusbio.com/fullsize/Bag-1-Antibody-2D3-Western-Blot-H00000573-M02-img0023.jpg)
![Western Blot: Bag-1 Antibody (2D3) [H00000573-M02] - BAG1 monoclonal antibody (M02), clone 2D3. Analysis of BAG1 expression in Jurkat.](http://images.novusbio.com/fullsize/Bag-1-Antibody-2D3-Western-Blot-H00000573-M02-img0024.jpg)
![Sandwich ELISA: Bag-1 Antibody (2D3) [H00000573-M02] - Detection limit for recombinant GST tagged BAG1 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Bag-1-Antibody-2D3-Sandwich-ELISA-H00000573-M02-img0019.jpg)
![Immunohistochemistry-Paraffin: Bag-1 Antibody [NB100-56081] - Formalin-fixed, paraffin-embedded sections of human colorectal cancer stained for Bag-1 using this antibody at 1:2000. Hematoxylin-eosin counterstain. Samples from two patients (A and B) are shown. A1 and B1 are higher magnifications from A and B, respectively.](http://images.novusbio.com/fullsize/Bag-1-Antibody-Immunohistochemistry-Paraffin-NB100-56081-img0004.jpg "Immunohistochemistry-Paraffin: Bag-1 Antibody [NB100-56081] - Formalin-fixed, paraffin-embedded sections of human colorectal cancer stained for Bag-1 using this antibody at 1:2000. Hematoxylin-eosin counterstain. Samples from two patients (A and B) are shown. A1 and B1 are higher magnifications from A and B, respectively.")
![Immunohistochemistry-Paraffin: Bag-1 Antibody [NB100-56081] - Formalin-fixed, paraffin-embedded section of human colon stained for Bag-1 using this antibody at 1:2000. Hematoxylin-eosin counterstain.](http://images.novusbio.com/fullsize/Bag-1-Antibody-Immunohistochemistry-Paraffin-NB100-56081-img0003.jpg)
![Simple Western: Bag-1 Antibody [NB100-56086] - Simple Western lane view shows a specific band for Bag-1 in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Bag-1-Antibody-Simple-Western-NB100-56086-img0001.jpg "Simple Western: Bag-1 Antibody [NB100-56086] - Simple Western lane view shows a specific band for Bag-1 in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![Western Blot: Bag-1 Antibody (JE63-22) [NBP3-32068] - Western blot analysis of Bag-1 on different lysates with Rabbit anti-Bag-1 antibody (NBP3-32068) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: Jurkat cell lysate (20 ug/Lane) Lane 3: HEK-293 cell lysate (20 ug/Lane) Lane 4: MCF7 cell lysate (20 ug/Lane) Lane 5: RAW264.7 cell lysate (20 ug/Lane) Lane 6: Rat testis tissue lysate (40 ug/Lane) Lane 7: Mouse testis tissue lysate (40 ug/Lane) Predicted band size: 38 kDa Observed band size: 35/46/52 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32068) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32068_rabbit-bag-1-mab-je63-22-27820241631691.jpg "Western Blot: Bag-1 Antibody (JE63-22) [NBP3-32068] - Western blot analysis of Bag-1 on different lysates with Rabbit anti-Bag-1 antibody (NBP3-32068) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: Jurkat cell lysate (20 ug/Lane) Lane 3: HEK-293 cell lysate (20 ug/Lane) Lane 4: MCF7 cell lysate (20 ug/Lane) Lane 5: RAW264.7 cell lysate (20 ug/Lane) Lane 6: Rat testis tissue lysate (40 ug/Lane) Lane 7: Mouse testis tissue lysate (40 ug/Lane) Predicted band size: 38 kDa Observed band size: 35/46/52 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32068) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Bag-1 Antibody (JE63-22) [NBP3-32068] - Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-Bag-1 antibody (NBP3-32068) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32068) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32068_rabbit-bag-1-mab-je63-22-298202414304135.jpg)
![Immunocytochemistry/ Immunofluorescence: Bag-1 Antibody (JE63-22) [NBP3-32068] - Immunocytochemistry analysis of HeLa cells labeling Bag-1 with Rabbit anti-Bag-1 antibody (NBP3-32068) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Bag-1 antibody (NBP3-32068) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32068_rabbit-bag-1-mab-je63-22-29202410334084.jpg)
![Flow Cytometry: Bag-1 Antibody (JE63-22) [NBP3-32068] - Flow cytometric analysis of HeLa cells labeling Bag-1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32068, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32068_rabbit-bag-1-mab-je63-22-29202416372155.jpg)
![Western Blot: Bag-1 Antibody (RM310) [NBP2-77409] - Western Blot of HeLa cell lysate, using NBP2-77409.](http://images.novusbio.com/fullsize/Bag-1-Antibody-RM310-Western-Blot-NBP2-77409-img0004.jpg "Western Blot: Bag-1 Antibody (RM310) [NBP2-77409] - Western Blot of HeLa cell lysate, using NBP2-77409.")
![Immunohistochemistry: Bag-1 Antibody (RM310) [NBP2-77409] - Immunohistochemical staining of formalin fixed and paraffin embedded BAG-1L overexpressing LNCaP cell section using NBP2-77409.](http://images.novusbio.com/fullsize/Bag-1-Antibody-RM310-Immunohistochemistry-NBP2-77409-img0003.jpg)
![Immunohistochemistry: Bag-1 Antibody (RM310) [NBP2-77409] - Immunohistochemical staining of formalin fixed and paraffin embedded 22RV1 cell section using NBP2-77409.](http://images.novusbio.com/fullsize/Bag-1-Antibody-RM310-Immunohistochemistry-NBP2-77409-img0001.jpg)
![Immunohistochemistry: Bag-1 Antibody (RM310) [NBP2-77409] - Immunohistochemical staining of formalin fixed and paraffin embedded 22RV1 cell section using NBP2-77409.](http://images.novusbio.com/fullsize/Bag-1-Antibody-RM310-Immunohistochemistry-NBP2-77409-img0002.jpg)
![Western Blot: Bag-1 Antibody (1E4B5) [NBP2-61698] - Analysis using BAG1 mAb against human BAG1 (AA: 219-346) recombinant protein. (Expected MW is 40.6 kDa)](http://images.novusbio.com/fullsize/Bag-1-Antibody-1E4B5-Western-Blot-NBP2-61698-img0004.jpg "Western Blot: Bag-1 Antibody (1E4B5) [NBP2-61698] - Analysis using BAG1 mAb against human BAG1 (AA: 219-346) recombinant protein. (Expected MW is 40.6 kDa)")
![Immunocytochemistry/Immunofluorescence: Bag-1 Antibody (1E4B5) [NBP2-61698] - Analysis of Hela cells using BAG1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/Bag-1-Antibody-1E4B5-Immunocytochemistry-Immunofluorescence-NBP2-61698-img0003.jpg)
![Flow Cytometry: Bag-1 Antibody (1E4B5) [NBP2-61698] - Analysis of Hela cells using BAG1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Bag-1-Antibody-1E4B5-Flow-Cytometry-NBP2-61698-img0002.jpg)
![Western Blot: Bag-1 Antibody (1E4B5) [NBP2-61698] - Analysis using BAG1 mAb against HEK293 (1) and BAG1 (AA: 219-346)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Bag-1-Antibody-1E4B5-Western-Blot-NBP2-61698-img0005.jpg)
![ELISA: Bag-1 Antibody (1E4B5) [NBP2-61698] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/Bag-1-Antibody-1E4B5-ELISA-NBP2-61698-img0001.jpg)
![Immunohistochemistry-Paraffin: Bag-1 Antibody [NBP1-89547] - Staining in human thyroid gland and pancreas tissues using anti-BAG1 antibody. Corresponding BAG1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Bag-1-Antibody-Immunohistochemistry-Paraffin-NBP1-89547-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Bag-1 Antibody [NBP1-89547] - Staining in human thyroid gland and pancreas tissues using anti-BAG1 antibody. Corresponding BAG1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Bag-1 Antibody [NBP1-89547] - Staining of human breast shows moderate positivity in glandular cells.](http://images.novusbio.com/fullsize/Bag-1-Antibody-Immunohistochemistry-Paraffin-NBP1-89547-img0002.jpg)
![Immunohistochemistry-Paraffin: Bag-1 Antibody [NBP1-89547] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/Bag-1-Antibody-Immunohistochemistry-Paraffin-NBP1-89547-img0005.jpg)
![Immunohistochemistry-Paraffin: Bag-1 Antibody [NBP1-89547] - Staining of human thyroid gland shows high expression.](http://images.novusbio.com/fullsize/Bag-1-Antibody-Immunohistochemistry-Paraffin-NBP1-89547-img0006.jpg)
![Western Blot: Bag-1 Antibody (SR1542) [NBP3-22527] - Western blot analysis using NBP3-22527 in HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-22527_rabbit-bag-1-mab-sr1542-57202315123418.jpg "Western Blot: Bag-1 Antibody (SR1542) [NBP3-22527] - Western blot analysis using NBP3-22527 in HeLa cell lysate.")