ATP6V1B2 Antibody (OTI1E11) - Azide and BSA Free

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Western Blot: ATP6V1B2 Antibody (OTI1E11) - Azide and BSA Free [NBP2-70238] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATP6V1B2.
Immunohistochemistry: ATP6V1B2 Antibody (OTI1E11) - Azide and BSA Free [NBP2-70238] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: ATP6V1B2 Antibody (OTI1E11) - Azide and BSA Free [NBP2-70238] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: ATP6V1B2 Antibody (OTI1E11) - Azide and BSA Free [NBP2-70238] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: ATP6V1B2 Antibody (OTI1E11) - Azide and BSA Free [NBP2-70238] - Analysis of Carcinoma of Human liver. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: ATP6V1B2 Antibody (OTI1E11) - Azide and BSA Free [NBP2-70238] - Analysis of Carcinoma of Human lung tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: ATP6V1B2 Antibody (OTI1E11) - Azide and BSA Free [NBP2-70238] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: ATP6V1B2 Antibody (OTI1E11) - Azide and BSA Free [NBP2-70238] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
OTI1E11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

ATP6V1B2 Antibody (OTI1E11) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human ATP6V1B2 (NP_001684) produced in HEK293T cell.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
ATP6V1B2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
Theoretical MW
56.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for ATP6V1B2 Antibody (OTI1E11) - Azide and BSA Free

  • 000 subunit
  • 56/58kD, isoform 2
  • ATP6B2ATPase, H+ transporting, lysosomal (vacuolar proton pump), beta polypeptide
  • ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B2
  • Endomembrane proton pump 58 kDa subunit
  • H+ transporting two-sector ATPase
  • HO57ATP6B1B2
  • vacuolar H+-ATPase 56
  • Vacuolar proton pump subunit B 2
  • VATB
  • V-ATPase B2 subunit
  • V-ATPase subunit B 2
  • Vma2
  • VPP3ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B, isoform 2
  • V-type proton ATPase subunit B, brain isoform

Background

The ATP6V1B2 gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification ofeukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellularprocesses as protein sortin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol ATP6V1B2