![Western Blot: APP Antibody (OTI7G9) [NBP2-02358] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Amyloid Precursor Protein (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Amyloid Precursor Protein.](http://images.novusbio.com/fullsize/Amyloid-Precursor-Protein-Antibody-7G9-Western-Blot-NBP2-02358-img0002.jpg "Western Blot: APP Antibody (OTI7G9) [NBP2-02358] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Amyloid Precursor Protein (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Amyloid Precursor Protein.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC |
| Clone | OTI7G9 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | Full length human recombinant protein of human APP (NP_000475) produced in HEK293T cell. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | APP |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 87 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 1.0% BSA and 50% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Immunogen affinity purified |
![Western Blot RFC1 Antibody [Unconjugated]](https://images.novusbio.com/images2/RFC1_AF6457_Western_Blot_9874.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for APP Antibody (NBP2-02358)Find related products by research area.
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HIV-associated neurocognitive disorders involve extracellular Nef-induced modification of lipid rafts and redistribution of Alzheimer’s disease-related proteins Jamshed Arslan, Pharm D, PhD Cholesterol is an essential part of animal cell membranes. Cholesterol-rich lipid rafts maintain the fluidity and protein trafficking of plasma membranes. Cellular ABCA1 protein moves cho... Read full blog post. |
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Mechanisms of Neurodegeneration: Protein aggregation and failure of autophagy By Michalina Hanzel, PhDIn a series of three blog posts I will briefly explore the major cellular mechanisms responsible for many neurodegenerative disorders. The first, and perhaps the most apparent, is the accumulat... Read full blog post. |
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Losing memory: Toxicity from mutant APP and amyloid beta explain the hippocampal neuronal damage in Alzheimer's disease By Jamshed Arslan Pharm.D. Alzheimer's disease (AD) is an irreversible brain disorder that destroys memory and thinking skills. The telltale signs of AD brains are extracellular deposits of amy... Read full blog post. |
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Lysosomal Dysfunction is Linked to Exosomal Secretion By Christina Towers, PhD. Lysosomal Dysfunction and DiseaseLysosomes are highly acidic organelles that are critical for cellular function and indispensable for degradative pathways like autophagy and endocytosis.... Read full blog post. |
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Immunity’s flipside: Microglia promote Alzheimer’s pathology during inflammation By Jamshed Arslan Pharm.D. Microglia are brain's macrophages. In Alzheimer's disease (AD), microglia clear up protein aggregates called amyloid beta plaques. The connection between immune activation and AD is unclea... Read full blog post. |
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The C99 fragment of amyloid precursor protein (APP) Alzheimer’s Disease (AD) is a neurodegenerative disorder that is characterized by an abundance of the beta-amyloid peptide in the brain. When AD was first discovered, it was determined that beta-amyloid was produced as a result of the prote... Read full blog post. |
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Beta Amyloid (MOAB2) and the link between traumatic brain injury and Alzheimer’s disease An epidemiological association between traumatic brain injury (TBI) and Alzheimer's disease (AD) has long been established. Interestingly, an increase in beta amyloid (one hallmark of AD) directly following TBI has been observed. In fact, it h... Read full blog post. |
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Niemann Pick-C1 and cholesterol dynamics Niemann-Pick type C1 (NPC1) mediates low-density cholesterol transport from late endosomes and lysosomes to other areas of the cell via receptor mediation endocytosis. Although cholesterol moves freely inside the cell, it cannot independently expo... Read full blog post. |
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FANCD2 and DNA damage repair Fanconi anemia (FA) is a genetically inherited disorder that yields cytogenetic instability, hypersensitivity to DNA crosslinking compounds and defective DNA repair. A variety of genes have been identified within the FA pathway that are referred t... Read full blog post. |
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Beta Amyloid Neurotoxicity and Alzheimer's Disease A major histopathological hallmark of Alzheimer's disease (AD) is the presence of amyloid deposits in the parenchyma of the amygdala, hippocampus, and neocortex. The principal component of amyloid is beta amyloid (AB). The pathologic accumulation of A... Read full blog post. |
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![Immunocytochemistry/Immunofluorescence: APP Antibody (OTI7G9) [NBP2-02358] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Amyloid Precursor Protein.](http://images.novusbio.com/fullsize/APP-Antibody-7G9-Immunocytochemistry-Immunofluorescence-NBP2-02358-img0009.jpg "Immunocytochemistry/Immunofluorescence: APP Antibody (OTI7G9) [NBP2-02358] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Amyloid Precursor Protein.")
![Immunohistochemistry-Paraffin: APP Antibody (OTI7G9) [NBP2-02358] - Staining of paraffin-embedded prostate tissue using anti-Amyloid Precursor Protein mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Amyloid-Precursor-Protein-Antibody-7G9-Immunohistochemistry-Paraffin-NBP2-02358-img0007.jpg "Immunohistochemistry-Paraffin: APP Antibody (OTI7G9) [NBP2-02358] - Staining of paraffin-embedded prostate tissue using anti-Amyloid Precursor Protein mouse monoclonal antibody.")
![Flow Cytometry: APP Antibody (OTI7G9) [NBP2-02358] - HEK293T cells transfected with either pCMV6-ENTRY Amyloid Precursor Protein.(Red) or empty vector control plasmid (Blue) were immunostained with anti-Amyloid Precursor Protein mouse monoclonal, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Amyloid-Precursor-Protein-Antibody-7G9-Flow-Cytometry-NBP2-02358-img0001.jpg "Flow Cytometry: APP Antibody (OTI7G9) [NBP2-02358] - HEK293T cells transfected with either pCMV6-ENTRY Amyloid Precursor Protein.(Red) or empty vector control plasmid (Blue) were immunostained with anti-Amyloid Precursor Protein mouse monoclonal, and then analyzed by flow cytometry.")
![Immunocytochemistry/Immunofluorescence: APP Antibody (OTI7G9) [NBP2-02358] - Staining of HepG2 cells using anti-Amyloid Precursor Protein mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Amyloid-Precursor-Protein-Antibody-7G9-Immunocytochemistry-Immunofluorescence-NBP2-02358-img0003.jpg "Immunocytochemistry/Immunofluorescence: APP Antibody (OTI7G9) [NBP2-02358] - Staining of HepG2 cells using anti-Amyloid Precursor Protein mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: APP Antibody (OTI7G9) [NBP2-02358] - Staining of paraffin-embedded colon tissue using anti-Amyloid Precursor Protein mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Amyloid-Precursor-Protein-Antibody-7G9-Immunohistochemistry-Paraffin-NBP2-02358-img0004.jpg "Immunohistochemistry-Paraffin: APP Antibody (OTI7G9) [NBP2-02358] - Staining of paraffin-embedded colon tissue using anti-Amyloid Precursor Protein mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: APP Antibody (OTI7G9) [NBP2-02358] - Staining of paraffin-embedded Kidney tissue using anti-Amyloid Precursor Protein mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Amyloid-Precursor-Protein-Antibody-7G9-Immunohistochemistry-Paraffin-NBP2-02358-img0005.jpg "Immunohistochemistry-Paraffin: APP Antibody (OTI7G9) [NBP2-02358] - Staining of paraffin-embedded Kidney tissue using anti-Amyloid Precursor Protein mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: APP Antibody (OTI7G9) [NBP2-02358] - Staining of paraffin-embedded liver tissue using anti-Amyloid Precursor Protein mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Amyloid-Precursor-Protein-Antibody-7G9-Immunohistochemistry-Paraffin-NBP2-02358-img0006.jpg "Immunohistochemistry-Paraffin: APP Antibody (OTI7G9) [NBP2-02358] - Staining of paraffin-embedded liver tissue using anti-Amyloid Precursor Protein mouse monoclonal antibody.")
![SDS-PAGE TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNF-alpha_210-TA_256.jpg)
![Bioactivity TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNFalpha_210TA_1658.jpg)
![SEC-MALS TNF-alpha [Unconjugated]](https://images.novusbio.com/images/210-ta_recombinant-human-tnf-alpha-protein-sec-mals-35202312244..jpg)
![Bioactivity GM-CSF [Unconjugated]](https://images.novusbio.com/images/protein/7954-gmcf_recombinant-human-gm-csf-cho-expressed-protein-cf-bioactivity-272020121514.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images2/DATA_IL6_M6000_ELISA_936.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images2/IL-6_M6000_ELISA_415.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images/m6000b_mouse-il-6-quantikine-elisa-kit-44202415433118.jpg)
![Immunocytochemistry Caspase-3 Antibody [Unconjugated] - Active](https://images.novusbio.com/images2/Caspase-3_AF835_Immunocytochemistry_6532.jpg)
![Immunohistochemistry Caspase-3 Antibody [Unconjugated] - Active](https://images.novusbio.com/images2/Caspase3_AF835_Immunohistochemistry_22976.jpg)
![Immunocytochemistry Caspase-3 Antibody [Unconjugated] - Active](https://images.novusbio.com/images2/Caspase-3_AF835_Immunocytochemistry_9340.jpg)
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")