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Annexin A1 Antibody (ANXA1/3566) - Azide and BSA Free

Images

 
Western Blot: Annexin A1 Antibody (ANXA1/3566) [NBP3-08708] - Western Blot Analysis of A549 cell lysate using Annexin A1 Mouse Monoclonal Antibody (Clone ANXA1/3566).
Immunocytochemistry/ Immunofluorescence: Annexin A1 Antibody (ANXA1/3566) [NBP3-08708] - Immunofluorescence staining of paraformaldehyde-fixed HeLa cells with Annexin A1 Mouse Monoclonal Antibody (ANXA1/3566) followed ...read more
Immunohistochemistry-Paraffin: Annexin A1 Antibody (ANXA1/3566) [NBP3-08708] - Formalin-fixed, paraffin-embedded human Lymph Node stained with Annexin A1 Mouse Monoclonal Antibody (ANXA1/3566).
Flow Cytometry: Annexin A1 Antibody (ANXA1/3566) [NBP3-08708] - Flow Cytometric Analysis of PFA-fixed HeLa cells using Annexin A1 Mouse Monoclonal Antibody (ANXA1/3566) followed by goat anti- Mouse IgG-CF488 (Blue); ...read more
Immunohistochemistry-Paraffin: Annexin A1 Antibody (ANXA1/3566) [NBP3-08708] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Annexin A1 Mouse Monoclonal Antibody (ANXA1/3566).
Immunohistochemistry-Paraffin: Annexin A1 Antibody (ANXA1/3566) [NBP3-08708] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Annexin A1 Mouse Monoclonal Antibody (ANXA1/3566).
Immunohistochemistry-Paraffin: Annexin A1 Antibody (ANXA1/3566) [NBP3-08708] - Formalin-fixed, paraffin-embedded human Lymph Node stained with Annexin A1 Mouse Monoclonal Antibody (ANXA1/3566).
Protein Array: Annexin A1 Antibody (ANXA1/3566) [NBP3-08708] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Annexin A1 Mouse Monoclonal Antibody (ANXA1/3566).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, MA
Clone
ANXA1/3566
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1 mg/ml

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Annexin A1 Antibody (ANXA1/3566) - Azide and BSA Free Summary

Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07716).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Immunogen
Recombinant full-length human Annexin A1 protein (Uniprot: P04083)
Localization
Nuclear, Cytoplasm and Cell Surface
Marker
Hairy Cell Leukemia Marker
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
ANXA1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Protein Array
  • Western Blot 1-2 ug/ml
Application Notes
Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Theoretical MW
38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Buffer
10 mM PBS
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein A or G purified

Alternate Names for Annexin A1 Antibody (ANXA1/3566) - Azide and BSA Free

  • Annexin A1
  • annexin I (lipocortin I)
  • Annexin I
  • Annexin-1
  • ANX1
  • ANXA1
  • Calpactin II
  • calpactin-2
  • chromobindin-9
  • Lipocortin I
  • LPC1
  • LPC1annexin-1
  • p35
  • Phospholipase A2 inhibitory protein

Background

Annexin I, also known as Annexin A1, belongs to the annexin family of calcium dependent phospholipid binding proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Annexin A1 Antibody (NBP3-08708) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ANXA1