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AMCase/CHIA Antibody

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Western Blot: AMCase/CHIA Antibody [NBP2-15880] - Sample (30 ug of whole cell lysate) A: JurKat 10% SDS PAGE gel, diluted at 1:1000.
Western Blot: AMCase/CHIA Antibody [NBP2-15880] - Sample (30 ug of whole cell lysate) A: JurKat 10% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: AMCase/CHIA Antibody [NBP2-15880] - Analysis of methanol-fixed A431, using antibody at 1:500 dilution.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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AMCase/CHIA Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human AMCase/CHIA. The exact sequence is proprietary.
Localization
Secreted; Isoform 2: Cytoplasm; Isoform 3: Cytoplasm
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CHIA
Purity
Antigen Affinity-purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for AMCase/CHIA Antibody

  • acidic mammalian chitinase
  • AMCase
  • CHIA
  • CHIT2
  • CHIT2DKFZp313J1722
  • Chitinase, Acidic
  • EC 3.2.1.14
  • Lung-Specific Protein TSA1902
  • TSA1902AMCase

Background

Degrades chitin and chitotriose. May participate in the defense against nematodes, fungi and other pathogens. Plays a role in T helper cell type 2 (Th2) immune response. Contributes to the response to IL-13 and inflammation in response to IL-13. Stimulates chemokine production by pulmonary epithelial cells. Protects lung epithelial cells against apoptosis and promotes phosphorylation of AKT1. Its function in the inflammatory response and in protecting cells against apoptosis is inhibited by allosamidin, suggesting that the function of this protein depends on carbohydrate binding.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol CHIA
Uniprot