Home » AlphaB Crystallin/CRYAB » AlphaB Crystallin/CRYAB Antibodies » AlphaB Crystallin/CRYAB Antibody (CL10758)
Novus Biologicals products are now on bio-techne.com

AlphaB Crystallin/CRYAB Antibody (CL10758)

 Submit a Review

Images

 
(
Enlarge)
Staining of human cerebellum shows strong cytoplasmic positivity in oligodendrocytes.
Staining of human cerebellum shows strong cytoplasmic positivity in oligodendrocytes.
Staining of U-138 MG cells using the Anti-CRYAB monoclonal antibody, showing specific staining in nuclear speckles in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Orthogonal Strategies: Analysis in human cerebral cortex and liver tissues using NBP3-21180 antibody. Corresponding CRYAB RNA-seq data are presented for the same tissues.
Analysis in human cell line U-2 OS.
Staining of human rectum shows strong cytoplasmic positivity in peripheral ganglia and nerves.
Staining of human liver shows no positivity in hepatocytes as expected.
Staining of human cerebral cortex shows strong cytoplasmic positivity in oligodendrocytes.
Staining of rat brain shows strong cytoplasmic positivity in oligodendrocytes.

Product Details

Summary
Reactivity Hu, Rt, MuSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
CL10758
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

  

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

AlphaB Crystallin/CRYAB Antibody (CL10758) Summary

Immunogen
This antibody was developed against a Synthetic Peptide of P02511. Actual immunogen sequence is proprietary.
Predicted Species
Mouse (100%). Backed by our 100% Guarantee.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CRYAB
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:20000 - 1:50000
  • Immunohistochemistry-Paraffin 1:20000 - 1:50000
  • Western Blot 1 ug/ml
Application Notes
ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.2, 40% glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A purified

Alternate Names for AlphaB Crystallin/CRYAB Antibody (CL10758)

  • alpha B crystallin
  • alpha(B)-crystallin
  • AlphaB Crystallin
  • alpha-crystallin B chain
  • CRYA2
  • CRYA2alpha crystallin B chain
  • CRYAB
  • crystallin, alpha B
  • CTPP2
  • Heat shock protein beta-5
  • heat-shock 20 kD like-protein
  • HSPB5
  • Renal carcinoma antigen NY-REN-27
  • Rosenthal fiber component

Background

Crystallin AB is a member of the small heat shock protein family and expressed in several tissues including brain, kidney cardiac and skeletal muscle. Crystallin AB functions as a molecular chaperone and in intracellular architecture. Crystallin AB has shown to have interactions with CRYAA, CRYGC, CRYBB2, CRYBA1 and HSPB8. Defects in crystalline AB have been linked to cause myopathy myofibrillar type 2, cataract posterior polar type 2, and myopathy myofibrillar fatal infantile hypertonic alpha-B crystallin-related (MFMFIH-CRYAB).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

HSP27 Antibody
(G3.1)
NBP2-32972
Flow Cytometry HSP27 Antibody (G3.1)
Western Blot HSP27 Antibody (G3.1)
Immunocytochemistry/ Immunofluorescence HSP27 Antibody (G3.1)
(1 Review)
(5 Publications)
Species: Ch, Pm, Hu, Pm, Mu, Rt, Sh
Applications: Flow, ICC/IF, IHC, IHC-P, WB
AlphaA
Crystallin/CRYAA Anti ...
NBP2-46354
Immunofluorescence AlphaA Crystallin/CRYAA Antibody (OTI3B12)
Immunohistochemistry AlphaA Crystallin/CRYAA Antibody (OTI3B12)
Immunohistochemistry AlphaA Crystallin/CRYAA Antibody (OTI3B12)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
Desmin Antibody
[Unconjugated]
AF3844
Immunohistochemistry Desmin Antibody [Unconjugated]
Immunohistochemistry Desmin Antibody [Unconjugated]
Immunocytochemistry/ Immunofluorescence Desmin Antibody [Unconjugated]
(2 Reviews)
(34 Publications)
Species: Hu, Mu
Applications: IHC
HSPB8/HSP22
Antibody (520817) [Un ...
MAB4987
Western Blot HSPB8/HSP22 Antibody (520817) [Unconjugated]
(5 Publications)
Species: Hu, Mu, Rt
Applications: WB
GFAP Antibody
NB300-141
Immunohistochemistry GFAP Antibody
Western Blot GFAP Antibody
Western Blot GFAP Antibody
(12 Reviews)
(108 Publications)
Species: Bv, Ch, Eq, Gp, Hu, Mu, Po, Rb, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
HSP20/HSPB6
Antibody (494310) [Un ...
MAB4200
Western Blot HSP20/HSPB6 Antibody (494310) [Unconjugated]
Simple Western HSP20/HSPB6 Antibody (494310) [Unconjugated]
(1 Publication)
Species: Hu, Mu, Rt
Applications: Simple Western, WB
HspA4 Antibody
NBP1-81696
Western Blot HspA4 Antibody
Immunohistochemistry-Paraffin HspA4 Antibody
Western Blot HspA4 Antibody
(1 Publication)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
Caspase-3 Antibody
[Unconjugated] - Acti ...
AF835
Immunocytochemistry Caspase-3 Antibody [Unconjugated] - Active
Immunohistochemistry Caspase-3 Antibody [Unconjugated] - Active
Immunocytochemistry Caspase-3 Antibody [Unconjugated] - Active
(7 Reviews)
(389 Publications)
Species: Hu, Mu
Applications: ICC, IHC
HSP10/EPF Antibody
[Unconjugated]
AF2584
Western Blot HSP10/EPF Antibody [Unconjugated]
Simple Western HSP10/EPF Antibody [Unconjugated]
Species: Mu
Applications: Simple Western, WB
CRYGD Antibody
(4E12)
H00001421-M03
Western Blot CRYGD Antibody (4E12)
Sandwich ELISA CRYGD Antibody (4E12)
Species: Hu
Applications: ELISA, S-ELISA, WB
CRYBB2 Antibody
(1F1)
H00001415-M02
Western Blot CRYBB2 Antibody (1F1)
Western Blot CRYBB2 Antibody (1F1)
ELISA CRYBB2 Antibody (1F1)
(1 Review)
(1 Publication)
Species: Hu
Applications: ELISA, ICC/IF, WB
gamma C Crystallin
Antibody - BSA Free
NBP3-03301
Western Blot gamma C Crystallin Antibody - BSA Free
Species: Hu, Mu, Rt
Applications: ICC/IF, WB
Myotilin Antibody
NBP2-38361
Immunohistochemistry-Paraffin Myotilin Antibody
Immunohistochemistry-Paraffin Myotilin Antibody
Immunohistochemistry-Paraffin Myotilin Antibody
Species: Hu
Applications: IHC, IHC-P
Insulin Antibody
(182410) [Unconjugate ...
MAB1417
Immunocytochemistry Insulin Antibody (182410) [Unconjugated]
Immunohistochemistry Insulin Antibody (182410) [Unconjugated]
(24 Publications)
Species: Bv, Hu, Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow
p53 Antibody (PAb
240) - BSA Free
NB200-103
Flow Cytometry p53 Antibody (PAb 240) - BSA Free
Flow Cytometry p53 Antibody (PAb 240) - BSA Free
Flow (Intracellular) p53 Antibody (PAb 240) - BSA Free
(3 Reviews)
(42 Publications)
Species: Hu, Mu, Rt, Xp(-), Ye
Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
alpha-Synuclein
Antibody
NBP2-15365
Immunohistochemistry-Frozen alpha-Synuclein Antibody
Immunohistochemistry-Frozen alpha-Synuclein Antibody
Immunocytochemistry/ Immunofluorescence alpha-Synuclein Antibody
(1 Review)
(12 Publications)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, S-ELISA, WB

Publications for AlphaB Crystallin/CRYAB Antibody (NBP3-21180) (0)

There are no publications for AlphaB Crystallin/CRYAB Antibody (NBP3-21180).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for AlphaB Crystallin/CRYAB Antibody (NBP3-21180) (0)

There are no reviews for AlphaB Crystallin/CRYAB Antibody (NBP3-21180). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for AlphaB Crystallin/CRYAB Antibody (NBP3-21180) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional AlphaB Crystallin/CRYAB Products

Research Areas for AlphaB Crystallin/CRYAB Antibody (NBP3-21180)

Find related products by research area.

Blogs on AlphaB Crystallin/CRYAB

There are no specific blogs for AlphaB Crystallin/CRYAB, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Western blot shows detection of Mouse Anti-Human/Mouse/Rat STAT3 Monoclonal Antibody (MAB1799) using a 1:1000 dilution of HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Goat anti-Mouse IgG Secondary Antibody [HRP]
HAF007
SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.
Mouse IgG1 Isotype Control (MG1)
NBP1-97005-0.5mg

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our AlphaB Crystallin/CRYAB Antibody (CL10758) and receive a gift card or discount.


Bioinformatics

Gene Symbol CRYAB