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alpha-Sarcoglycan Antibody (3C4)

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Western Blot: alpha-Sarcoglycan Antibody (3C4) [H00006442-M01] - Analysis of SGCA expression in transfected 293T cell line by SGCA monoclonal antibody (M01), clone 3C4.Lane 1: SGCA transfected lysate(42.9 KDa).Lane 2: ...read more
Sandwich ELISA: alpha-Sarcoglycan Antibody (3C4) [H00006442-M01] - Detection limit for recombinant GST tagged SGCA is 1 ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clone
3C4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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alpha-Sarcoglycan Antibody (3C4) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
SGCA (NP_000014.1, 26 a.a. ~ 133 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. TTLHPLVGRVFVHTLDHETFLSLPEHVAVPPAVHITYHAHLQGHPDLPRWLRYTQRSPHHPGFLYGSATPEDRGLQVIEVTAYNRDSFDTTRQRLVLEIGDPEGPLLP
Specificity
SGCA - sarcoglycan, alpha (50kDa dystrophin-associated glycoprotein) (3C4)
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
SGCA
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Sandwich ELISA
  • Western Blot
Application Notes
Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for ELISA.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for alpha-Sarcoglycan Antibody (3C4)

  • 50-DAG
  • Adhalin
  • ADL
  • alphaSarcoglycan
  • alpha-Sarcoglycan
  • DAG2
  • DMDA2
  • DMDA250kD DAG
  • Dystroglycan-2
  • LGMD2D
  • LGMD2Dalpha (50kD dystrophin-associated glycoprotein)
  • sarcoglycan, alpha (50kDa dystrophin-associated glycoprotein)
  • SCARMD1
  • SGCA

Background

The dystrophin-glycoprotein complex (DGC) comprises a group of proteins that are critical to the stability of muscle fiber membranes and to the linking of the actin cytoskeleton to the extracellular matrix. Components of the DGC include dystrophin (MIM 300377), which is deficient in Duchenne muscular dystrophy (DMD; MIM 310200); syntrophins (e.g., MIM 600026); dystroglycans (MIM 128239); and sarcoglycans, such as adhalin, a 50-kD transmembrane protein (Roberds et al., 1993 [PubMed 8226900]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol SGCA
Entrez
Uniprot