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Human alpha-Galactosidase A/GLA ELISA Kit (Colorimetric)

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ELISA: Human alpha-Galactosidase A/GLA ELISA Kit (Colorimetric) [NBP3-23444] - Samples were spiked with high concentrations of alpha-Galactosidase A/GLA and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Human alpha-Galactosidase A/GLA ELISA Kit (Colorimetric) [NBP3-23444] - Samples were spiked with high concentrations of alpha-Galactosidase A/GLA and diluted with Reference Standard & Sample Diluent to produce ...read more
ELISA: Human alpha-Galactosidase A/GLA ELISA Kit (Colorimetric) [NBP3-23444] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
0.78 - 50 ng/mL
Sensitivity
0.49 ng/mL

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Human alpha-Galactosidase A/GLA ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Human alpha-Galactosidase A/GLA in samples. No significant cross reactivity or interference between Human alpha-Galactosidase A/GLA and analogues was observed.
Standard Curve Range
0.78 - 50 ng/mL
Sensitivity
0.49 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
%CV < 5.88
Intra-Assay
%CV < 6.7
Spike Recovery
84 - 105%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
GLA

Applications/Dilutions

Dilutions
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Human alpha-Galactosidase A/GLA ELISA Kit (Colorimetric)

  • agalsidase alfa
  • Agalsidase alpha
  • Agalsidase
  • Alpha-D-galactosidase A
  • alpha-D-galactoside galactohydrolase 1
  • Alpha-D-galactoside galactohydrolase
  • alpha-gal A
  • alpha-galactosidase A
  • EC 3.2.1
  • EC 3.2.1.22
  • GALA
  • galactosidase, alpha
  • GLA
  • Melibiase

Background

Galactosidase alpha encodes a homodimeric glycoprotein that hydrolyses the terminal alpha-galactosyl moieties from glycolipids and glycoproteins. This enzyme predominantly hydrolyzes ceramide trihexoside, and it can catalyze the hydrolysis of melibiose into galactose and glucose. A variety of mutations in this gene affect the synthesis, processing, and stability of this enzyme, which causes Fabry disease, a rare lysosomal storage disorder that results from a failure to catabolize alpha-D-galactosyl glycolipid moieties. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Human alpha-Galactosidase A/GLA ELISA Kit (Colorimetric)
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  Manual - Human alpha-Galactosidase A/GLA ELISA Kit (Colorimetric)
  PDF Datasheet
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Bioinformatics

Gene Symbol GLA