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Human alpha-Galactosidase A/GLA ELISA Kit (Colorimetric)

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ELISA: Human alpha-Galactosidase A/GLA ELISA Kit (Colorimetric) [NBP2-82180] - Samples were spiked with high concentrations of Human alpha-Galactosidase A/GLA and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Human alpha-Galactosidase A/GLA ELISA Kit (Colorimetric) [NBP2-82180] - Samples were spiked with high concentrations of Human alpha-Galactosidase A/GLA and diluted with Reference Standard & Sample Diluent to ...read more
ELISA: Human alpha-Galactosidase A/GLA ELISA Kit (Colorimetric) [NBP2-82180] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, Plasma, Cell supernatant
Standard Curve Range
78.13 - 5000 pg/mL
Sensitivity
46.88 pg/mL

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Human alpha-Galactosidase A/GLA ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Human alpha-Galactosidase A/GLA in samples. No significant cross-reactivity or interference between Human alpha-Galactosidase A/GLA and analogues was observed.
Standard Curve Range
78.13 - 5000 pg/mL
Sensitivity
46.88 pg/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 6%
Intra-Assay
CV% < 5%
Spike Recovery
average = 95% for serum; average = 100% for EDTA plasma; average = 101% for cell culture media
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
GLA

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Micro ELISA Plate, dismountable (8 wells x 12 strips); Reference Standard (2 vials); Concentrated (100X) Biotinylated Detection Ab (120 uL); Concentrated (100X) HRP Conjugate (120 uL); Reference Standard & Sample Diluent (20 mL); Biotinylated Detection Ab

Alternate Names for Human alpha-Galactosidase A/GLA ELISA Kit (Colorimetric)

  • agalsidase alfa
  • Agalsidase alpha
  • Agalsidase
  • Alpha-D-galactosidase A
  • alpha-D-galactoside galactohydrolase 1
  • Alpha-D-galactoside galactohydrolase
  • alpha-gal A
  • alpha-galactosidase A
  • EC 3.2.1
  • EC 3.2.1.22
  • GALA
  • galactosidase, alpha
  • GLA
  • Melibiase

Background

Galactosidase alpha encodes a homodimeric glycoprotein that hydrolyses the terminal alpha-galactosyl moieties from glycolipids and glycoproteins. This enzyme predominantly hydrolyzes ceramide trihexoside, and it can catalyze the hydrolysis of melibiose into galactose and glucose. A variety of mutations in this gene affect the synthesis, processing, and stability of this enzyme, which causes Fabry disease, a rare lysosomal storage disorder that results from a failure to catabolize alpha-D-galactosyl glycolipid moieties. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Human alpha-Galactosidase A/GLA ELISA Kit (Colorimetric)
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  Human alpha-Galactosidase A/GLA ELISA Kit (Colorimetric) Manual
  PDF Datasheet
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Bioinformatics

Gene Symbol GLA