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14-3-3 epsilon Antibody (CPTC-YWHAE-1)

Images

 
Western Blot: 14-3-3 epsilon Antibody (CPTC-YWHAE-1) [NBP2-79810] - Western Blot Analysis of HEK293, PC-3 and A431 cell lysates using 14-3-3 epsilon Antibody (CPTC-YWHAE-1).
Immunohistochemistry-Paraffin: 14-3-3 epsilon Antibody (CPTC-YWHAE-1) [NBP2-79810] - Formalin-fixed, paraffin-embedded human Testis stained with 14-3-3 epsilon Antibody (CPTC-YWHAE-1).
Western Blot: 14-3-3 epsilon Antibody (CPTC-YWHAE-1) [NBP2-79810] - MDA-MB-231 and MCF-7 whole cell lysates were loaded (40 ug/lane). 10% SDS-PAGE. 14-3-3 epsilon Antibody (NBP2-79810) primary antibody at 1:1000, 4C, ...read more
Protein Array: 14-3-3 epsilon Antibody (CPTC-YWHAE-1) [NBP2-79810] - Analysis of Protein Array containing more than 19,000 full-length human proteins using 14-3-3 epsilon Antibody (CPTC-YWHAE-1). Z- and S- Score: The ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, MA
Clone
CPTC-YWHAE-1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

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14-3-3 epsilon Antibody (CPTC-YWHAE-1) Summary

Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-79944)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Immunogen
Recombinant human full-length 14-3-3 epsilon protein (Uniprot: P62268)
Localization
Cytoplasm, Melanosome
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
YWHAE
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1-2 ug/ml
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Protein Array
  • Western Blot 1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Theoretical MW
29.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-79810 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for 14-3-3 epsilon Antibody (CPTC-YWHAE-1)

  • 1433 epsilon
  • 14-3-3 epsilon
  • 14-3-3 protein epsilon
  • FLJ45465
  • FLJ53559,14-3-3E
  • KCIP-1
  • MDCR
  • MDS
  • mitochondrial import stimulation factor L subunit
  • protein kinase C inhibitor protein-1
  • tyrosine 3/tryptophan 5 -monooxygenase activation protein, epsilon polypeptide
  • tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilonpolypeptide
  • YWHAE

Background

14-3-3 epsilon product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for 14-3-3 epsilon Antibody (NBP2-79810) (0)

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Review for 14-3-3 epsilon Antibody (NBP2-79810) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-79810:
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WB
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Images Ratings Applications Species Date Details
Western Blot 14-3-3 epsilon NBP2-79810
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5
reviewed by:
Verified Customer
WB Human 03/06/2021
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Summary

ApplicationWestern Blot
Sample TestedBREAST CANCER CELL LINES, Sample Amount: 40 ug
SpeciesHuman
Lot75311P190906

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Secondary Antibodies

 

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Recent Reviews

5
5
1
4
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1
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Verified Customer
03/06/2021
Application: WB
Species: Human

Bioinformatics

Gene Symbol YWHAE
Uniprot