EDD Antibodies
We offer UBR5/EDD Antibodies for use in common research applications: ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Immunoprecipitation (Negative), Peptide ELISA, Western Blot. Each UBR5/EDD Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our UBR5/EDD Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the UBR5/EDD Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our UBR5/EDD polyclonal antibodies.
Alternate Names for EDD Antibodies
anti-UBR5/EDD antibody, anti-UBR5 antibody, anti-DD5 antibody, anti-E3 ubiquitin protein ligase, HECT domain containing, 1 antibody, anti-E3 ubiquitin-protein ligase, HECT domain-containing 1 antibody, anti-EC 6.3.2 antibody, anti-EC 6.3.2.- antibody, anti-EDD1E3 ubiquitin-protein ligase UBR5 antibody, anti-EDDE3 identified by differential display antibody, anti-hHYD antibody, anti-HYDFLJ11310 antibody, anti-Hyperplastic discs protein homolog antibody, anti-KIAA0896MGC57263 antibody, anti-progestin induced protein antibody, anti-Progestin-induced protein antibody, anti-ubiquitin protein ligase E3 component n-recognin 5 antibody, anti-ubiquitin-protein ligase antibody
9 Results for "ubr5-edd" in Primary Antibodies
![Western Blot: UBR5/EDD Antibody [NB100-1591] - Samples: Whole cell lysate (50 ug) from HEK293T, Jurkat, and LNCaP cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit UBR5/EDD Antibody (NB100-1591, lot 6) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/UBR5-EDD-Antibody-Western-Blot-NB100-1591-img0003.jpg "Western Blot: UBR5/EDD Antibody [NB100-1591] - Samples: Whole cell lysate (50 ug) from HEK293T, Jurkat, and LNCaP cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit UBR5/EDD Antibody (NB100-1591, lot 6) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunohistochemistry-Paraffin: UBR5/EDD Antibody [NB100-1591] - Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit UBR5/EDD Antibody (NB100-1591, lot 6) used at a dilution of 1:1,000 (1 ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/UBR5-EDD-Antibody-Immunohistochemistry-Paraffin-NB100-1591-img0002.jpg)
![Immunohistochemistry-Paraffin: UBR5/EDD Antibody [NB100-1591] - Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit UBR5/EDD Antibody (NB100-1591, lot 6) used at a dilution of 1:1000 (1 ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/UBR5-EDD-Antibody-Immunohistochemistry-Paraffin-NB100-1591-img0001.jpg)
![Immunoprecipitation: UBR5/EDD Antibody [NB100-1591] - Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit UBR5/EDD Antibody (NB100-1591, lot 6) used for IP at 6 ug per reaction. UBR5/EDD was also immunoprecipitated by a previous lot of this antibody (lot 5). For blotting immunoprecipitated UBR5/EDD, NB100-1591 was used at 0.1 ug/ml. Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/UBR5-EDD-Antibody-Immunoprecipitation-NB100-1591-img0004.jpg)
![Immunohistochemistry-Paraffin: UBR5/EDD Antibody [NB100-1220] - Staining of paraffin embedded Human Testis. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining. These results could not be obtained after antigen retrieval at pH6.](http://images.novusbio.com/fullsize/UBR5-EDD-Antibody-Immunohistochemistry-Paraffin-NB100-1220-img0002.jpg "Immunohistochemistry-Paraffin: UBR5/EDD Antibody [NB100-1220] - Staining of paraffin embedded Human Testis. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining. These results could not be obtained after antigen retrieval at pH6.")
![Western Blot: UBR5/EDD Antibody (JE38-53) [NBP3-32291] - Western blot analysis of UBR5/EDD on different lysates with Rabbit anti-UBR5/EDD antibody (NBP3-32291) at 1/1,000 dilution. Lane 1: Neuro-2a cell lysate Lane 2: SH-SY5Y cell lysate Lane 3: Jurkat cell lysate Lane 4: A549 cell lysate Lane 5: HEK-293 cell lysate Lane 6: LNCaP cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 309 kDa Observed band size: 309 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32291) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32291_rabbit-ubr5-edd-mab-je38-53-278202415242622.jpg "Western Blot: UBR5/EDD Antibody (JE38-53) [NBP3-32291] - Western blot analysis of UBR5/EDD on different lysates with Rabbit anti-UBR5/EDD antibody (NBP3-32291) at 1/1,000 dilution. Lane 1: Neuro-2a cell lysate Lane 2: SH-SY5Y cell lysate Lane 3: Jurkat cell lysate Lane 4: A549 cell lysate Lane 5: HEK-293 cell lysate Lane 6: LNCaP cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 309 kDa Observed band size: 309 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32291) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: UBR5/EDD Antibody (JE38-53) [NBP3-32291] - Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-UBR5/EDD antibody (NBP3-32291) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32291) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32291_rabbit-ubr5-edd-mab-je38-53-29820241435268.jpg)
![Immunocytochemistry/ Immunofluorescence: UBR5/EDD Antibody (JE38-53) [NBP3-32291] - Immunocytochemistry analysis of SH-SY5Y cells labeling UBR5/EDD with Rabbit anti-UBR5/EDD antibody (NBP3-32291) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-UBR5/EDD antibody (NBP3-32291) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32291_rabbit-ubr5-edd-mab-je38-53-292024119234.jpg)
![Western Blot: UBR5/EDD Antibody [NBP3-12948] - U87-MG whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with UBR5/EDD antibody (NBP3-12948) diluted at 1:500.](http://images.novusbio.com/fullsize/UBR5-EDD-Antibody-Western-Blot-NBP3-12948-img0001.jpg "Western Blot: UBR5/EDD Antibody [NBP3-12948] - U87-MG whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with UBR5/EDD antibody (NBP3-12948) diluted at 1:500.")
![Immunocytochemistry/Immunofluorescence: UBR5/EDD Antibody [NBP3-12948] - UBR5/EDD antibody detects UBR5/EDD protein at nucleus by immunofluorescent analysis. Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: UBR5/EDD protein stained by UBR5/EDD antibody (NBP3-12948) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/UBR5-EDD-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-12948-img0002.jpg)
![UBR5/EDD Antibody [NBP1-71802] - Detection of human and mouse EDD1 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (H; 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (5, 15, and 50 ug for WB) and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-EDD1 antibody NBP1-71802 used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. EDD1 was less efficiently immunoprecipitated by another rabbit anti-EDD1 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).](http://images.novusbio.com/fullsize/UBR5-EDD-Antibody-NBP1-71802-img0003.jpg "UBR5/EDD Antibody [NBP1-71802] - Detection of human and mouse EDD1 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (H; 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (5, 15, and 50 ug for WB) and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-EDD1 antibody NBP1-71802 used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. EDD1 was less efficiently immunoprecipitated by another rabbit anti-EDD1 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).")
![Western Blot: UBR5/EDD Antibody (SR1501) [NBP3-22217] - Western blot analysis using NBP3-22217 in SH-SY5Y cell lysate.](http://images.novusbio.com/fullsize/nbp3-22217_rabbit-ubr5-edd-mab-sr1501-5720231624740.jpg "Western Blot: UBR5/EDD Antibody (SR1501) [NBP3-22217] - Western blot analysis using NBP3-22217 in SH-SY5Y cell lysate.")
![Immunocytochemistry/Immunofluorescence: UBR5/EDD Antibody [NBP2-58579] - Staining of human cell line MCF7 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/UBR5-EDD-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58579-img0001.jpg "Immunocytochemistry/Immunofluorescence: UBR5/EDD Antibody [NBP2-58579] - Staining of human cell line MCF7 shows localization to nucleoplasm.")