SOX10 Antibodies
We offer SOX10 Antibodies for use in common research applications: ELISA, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot. Each SOX10 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our SOX10 Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the SOX10 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our SOX10 polyclonal antibodies and browse our SOX10 monoclonal antibody catalog.
Alternate Names for SOX10 Antibodies
anti-SOX10 antibody, anti-MGC15649 antibody, anti-PCWH antibody, anti-SRY (sex determining region Y)-box 10 antibody, anti-SRY-related HMG-box gene 10 antibody, anti-transcription factor SOX-10 antibody, anti-WS4C antibody, anti-WS4mouse, human homolog of antibody
21 Results for "sox10" in Primary Antibodies
![Immunocytochemistry/Immunofluorescence: SOX10 Antibody (CL4455) [NBP2-59050] - staining of SK-MEL-30 cells using the Anti-SOX10 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/SOX10-Antibody-CL4455-Immunocytochemistry-Immunofluorescence-NBP2-59050-img0011.jpg "Immunocytochemistry/Immunofluorescence: SOX10 Antibody (CL4455) [NBP2-59050] - staining of SK-MEL-30 cells using the Anti-SOX10 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.")
![Immunohistochemistry-Frozen: SOX10 Antibody (CL4455) [NBP2-59050] - Feline adult spinal cord demonstrating SOX10 (red) and DAPI (blue). Antibody at 1:200 dilutiion in 1X PBS + 0.05% tween 20+ 2% BSA. Tested at 1:50, 1:100, 1:200, 1:500, and 1:1000. Dilution of 1:200 gave the best signal/noise ratio for IHC-Fr. The slides were subsequently incubated with Alexa Fluor conjugated secondary antibody for 1hr at RT and imaged with fluorescent microscopy. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/SOX10-Antibody-CL4455-Immunohistochemistry-Frozen-NBP2-59050-img0013.jpg)
![Immunohistochemistry: SOX10 Antibody (CL4455) [NBP2-59050] - Staining of mouse embryo E11 shows nuclear immunoreactivity in the developing trigeminal ganglion and ear placode.](http://images.novusbio.com/fullsize/SOX10-Antibody-CL4455-Immunohistochemistry-NBP2-59050-img0002.jpg)
![Immunohistochemistry-Paraffin: SOX10 Antibody (CL4455) [NBP2-59050] - Staining of human cerebral cortex shows strong nuclear immunoreactivity in a subset of glial cells.](http://images.novusbio.com/fullsize/SOX10-Antibody-CL4455-Immunohistochemistry-Paraffin-NBP2-59050-img0004.jpg)
![Immunohistochemistry-Paraffin: SOX10 Antibody (CL4455) [NBP2-59050] - Staining of human breast shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/SOX10-Antibody-CL4455-Immunohistochemistry-Paraffin-NBP2-59050-img0005.jpg)
![Immunohistochemistry-Paraffin: SOX10 Antibody (CL4455) [NBP2-59050] - Staining of human cervix shows nuclear immunoreactivity in a subset of basal cells in squamous epithelium.](http://images.novusbio.com/fullsize/SOX10-Antibody-CL4455-Immunohistochemistry-Paraffin-NBP2-59050-img0009.jpg)
![Immunohistochemistry-Paraffin: SOX10 Antibody (CL4455) [NBP2-59050] - Staining of human pancreas shows absence of positivity as expected (negative control).](http://images.novusbio.com/fullsize/SOX10-Antibody-CL4455-Immunohistochemistry-Paraffin-NBP2-59050-img0007.jpg)
![Protein Array: SOX10 Antibody (SOX10/991) [NBP2-59620] - containing more than 19,000 full-length human proteins. Z- and S- Score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary) produces when binding to a particular protein on the HuProt array. Z-scores are described in units of standard deviations above the mean value of all signals generated on that array. If targets on HuProt are arranged in descending order of the Z-score, the S-score is the difference between the Z-score. S-score therefore represents the relative target specificity of an Ab to its intended target. An Ab is considered to specific to its intended target, if the Ab has an S-score of at least 2.5. For example, if Ab binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Ab to protein X is equal to 29](http://images.novusbio.com/fullsize/SOX10-Antibody-SOX10-991-Protein-Array-NBP2-59620-img0001.jpg "Protein Array: SOX10 Antibody (SOX10/991) [NBP2-59620] - containing more than 19,000 full-length human proteins. Z- and S- Score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary) produces when binding to a particular protein on the HuProt array. Z-scores are described in units of standard deviations above the mean value of all signals generated on that array. If targets on HuProt are arranged in descending order of the Z-score, the S-score is the difference between the Z-score. S-score therefore represents the relative target specificity of an Ab to its intended target. An Ab is considered to specific to its intended target, if the Ab has an S-score of at least 2.5. For example, if Ab binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Ab to protein X is equal to 29")
![Western Blot: SOX10 Antibody (SOX10/991) [NBP2-59620] - Western Blot Analysis of COLO-38 cell lysate usingSOX10 antibody (SOX10/991).](http://images.novusbio.com/fullsize/SOX10-Antibody-SOX10-991-Western-Blot-NBP2-59620-img0008.jpg)
![Immunohistochemistry-Paraffin: SOX10 Antibody (SOX10/991) [NBP2-59620] - Formalin-fixed, paraffin-embedded Human Melanoma stained with SOX10 Mouse Monoclonal Antibody (SOX10/991).](http://images.novusbio.com/fullsize/SOX10-Antibody-SOX10-991-Immunohistochemistry-Paraffin-NBP2-59620-img0004.jpg)
![Western Blot: SOX10 Antibody (SOX10/991) [NBP2-59620] - analysis of SOX10 (A) Recombinant protein (B) A375 Cell lysate using SOX10 Mouse Monoclonal Antibody (SOX10/991).](http://images.novusbio.com/fullsize/SOX10-Antibody-SOX10-991-Western-Blot-NBP2-59620-img0003.jpg)
![Immunohistochemistry-Paraffin: SOX10 Antibody (SOX10/991) [NBP2-59620] - Formalin-fixed, paraffin-embedded Mouse Brain stained with SOX10 Mouse Monoclonal Antibody (SOX10/991).](http://images.novusbio.com/fullsize/SOX10-Antibody-SOX10-991-Immunohistochemistry-Paraffin-NBP2-59620-img0002.jpg)
![Simple Western: SOX10 Antibody (SOX10/991) [NBP2-59620] - Analysis of SOX10 antibody on mouse hippocampus sample. Primary antibody dilution: 1:50.1 ug/uL protein. Image from verified customer review.](http://images.novusbio.com/fullsize/nbp2-59620_mouse-monoclonal-sox10-antibody-sox10-991-simple-western-284202313501.jpg)
![Western Blot: SOX10 Antibody [NBP1-68983] - Analysis of HepG2 lysate Cell lysates, Antibody Dilution: 0.5 ug/ml.](http://images.novusbio.com/fullsize/SOX10-Antibody-Western-Blot-NBP1-68983-img0011.jpg "Western Blot: SOX10 Antibody [NBP1-68983] - Analysis of HepG2 lysate Cell lysates, Antibody Dilution: 0.5 ug/ml.")
![Immunohistochemistry-Paraffin: SOX10 Antibody [NBP1-68983] - Human Kidney tissue, Epithelial cells of renal tubule (Indicated with Arrows) 4-8 ug/ml.](http://images.novusbio.com/fullsize/SOX10-Antibody-Immunohistochemistry-Paraffin-NBP1-68983-img0005.jpg)
![Western Blot: SOX10 Antibody [NBP1-68983] - HepG2, 0.25ug/ml.](http://images.novusbio.com/fullsize/SOX10-Antibody-Western-Blot-NBP1-68983-img0001.jpg)
![Western Blot: SOX10 Antibody [NBP1-68983] - SOX10 antibody - middle region validated by WB using Hek 293 Whole Cell Lysate at 1:4,000.](http://images.novusbio.com/fullsize/SOX10-Antibody-Western-Blot-NBP1-68983-img0006.jpg)
![Western Blot: SOX10 Antibody [NBP1-68983] - Sample Tissue: Jurkat cell lysates Lane A: Primary Antibody Lane B: Primary Antibody + Blocking Peptide Primary Antibody Concentration:2.5ug/ml Peptide Concentration: 4.0ug/ml Lysate Quantity: 25ug/lane Gel Concentration:](http://images.novusbio.com/fullsize/SOX10-Antibody-Western-Blot-NBP1-68983-img0010.jpg)
![Immunohistochemistry-Paraffin: SOX10 Antibody [NBP1-68983] - Human Kidney Tissue. Arrow indicates epithelial cells of renal tubule with positive label.](http://images.novusbio.com/fullsize/SOX10-Antibody-Immunohistochemistry-Paraffin-NBP1-68983-img0002.jpg)
![Immunohistochemistry-Paraffin: SOX10 Antibody [NBP1-68983] - Human Skin Tissue with epidermal cells positively labeled.](http://images.novusbio.com/fullsize/SOX10-Antibody-Immunohistochemistry-Paraffin-NBP1-68983-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: SOX10 Antibody (SD204-04) [NBP2-67812] - Staining SOX10 in NCCIT cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/SOX10-Antibody-SD204-04-Immunocytochemistry-Immunofluorescence-NBP2-67812-img0004.jpg "Immunocytochemistry/Immunofluorescence: SOX10 Antibody (SD204-04) [NBP2-67812] - Staining SOX10 in NCCIT cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: SOX10 Antibody (SD204-04) [NBP2-67812] - Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-SOX10 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/SOX10-Antibody-SD204-04-Immunohistochemistry-Paraffin-NBP2-67812-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: SOX10 Antibody (SD204-04) [NBP2-67812] - Staining SOX10 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/SOX10-Antibody-SD204-04-Immunocytochemistry-Immunofluorescence-NBP2-67812-img0001.jpg)
![Immunohistochemistry-Paraffin: SOX10 Antibody (SD204-04) [NBP2-67812] - Analysis of paraffin-embedded mouse brain tissue using anti-SOX10 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/SOX10-Antibody-SD204-04-Immunohistochemistry-Paraffin-NBP2-67812-img0002.jpg)
![Immunohistochemistry-Paraffin: SOX10 Antibody (SD204-04) [NBP2-67812] - Analysis of paraffin-embedded rat brain tissue using anti-SOX10 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/SOX10-Antibody-SD204-04-Immunohistochemistry-Paraffin-NBP2-67812-img0003.jpg)
![Immunohistochemistry-Paraffin: SOX10 Antibody (SD204-04) [NBP2-67812] - Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-SOX10 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/SOX10-Antibody-SD204-04-Immunohistochemistry-Paraffin-NBP2-67812-img0005.jpg)
![Immunohistochemistry-Paraffin: SOX10 Antibody (SD204-04) [NBP2-67812] - Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-SOX10 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/SOX10-Antibody-SD204-04-Immunohistochemistry-Paraffin-NBP2-67812-img0006.jpg)
![Protein Array: SOX10 Antibody (SOX10/1074) [NBP2-59622] - containing more than 19,000 full-length human proteins. Z- and S- Score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary) produces when binding to a particular protein on the HuProt array. Z-scores are described in units of standard deviations above the mean value of all signals generated on that array. If targets on HuProt are arranged in descending order of the Z-score, the S-score is the difference between the Z-score. S-score therefore represents the relative target specificity of an Ab to its intended target. An Ab is considered to specific to its intended target, if the Ab has an S-score of at least 2.5. For example, if Ab binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Ab to protein X is equal to 29](http://images.novusbio.com/fullsize/SOX10-Antibody-SOX10-1074-Protein-Array-NBP2-59622-img0001.jpg "Protein Array: SOX10 Antibody (SOX10/1074) [NBP2-59622] - containing more than 19,000 full-length human proteins. Z- and S- Score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary) produces when binding to a particular protein on the HuProt array. Z-scores are described in units of standard deviations above the mean value of all signals generated on that array. If targets on HuProt are arranged in descending order of the Z-score, the S-score is the difference between the Z-score. S-score therefore represents the relative target specificity of an Ab to its intended target. An Ab is considered to specific to its intended target, if the Ab has an S-score of at least 2.5. For example, if Ab binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Ab to protein X is equal to 29")
![Western Blot: SOX10 Antibody (SOX10/1074) [NBP2-59622] - Western blot analysis of COLO-38 cell lysate using SOX10 antibody (SOX10/1074).](http://images.novusbio.com/fullsize/SOX10-Antibody-SOX10-1074-Western-Blot-NBP2-59622-img0005.jpg)
![Immunohistochemistry-Paraffin: SOX10 Antibody (SOX10/1074) [NBP2-59622] - Formalin-fixed, paraffin-embedded Human Melanoma stained with SOX10 Mouse Monoclonal Antibody (SOX10/1074).](http://images.novusbio.com/fullsize/SOX10-Antibody-SOX10-1074-Immunohistochemistry-Paraffin-NBP2-59622-img0004.jpg)
![Western Blot: SOX10 Antibody (SOX10/1074) [NBP2-59622] - analysis of SOX10 (A) Recombinant protein; (B) A375 Cell lysate using SOX10 Mouse Monoclonal Antibody (SOX10/1074)](http://images.novusbio.com/fullsize/SOX10-Antibody-SOX10-1074-Western-Blot-NBP2-59622-img0003.jpg)
![Immunohistochemistry-Paraffin: SOX10 Antibody (SOX10/1074) [NBP2-59622] - Formalin-fixed, paraffin-embedded Mouse Brain stained with SOX10 Mouse Monoclonal Antibody (SOX10/1074).](http://images.novusbio.com/fullsize/SOX10-Antibody-SOX10-1074-Immunohistochemistry-Paraffin-NBP2-59622-img0002.jpg)
![Simple Western: SOX10 Antibody (SOX10/1074) [NBP2-59622] - Analysis of SOX10 antibody on mouse hippocampus sample. Primary antibody dilution: 1:50. 1 ug/uL protein. Image from verified customer review.](http://images.novusbio.com/fullsize/nbp2-59622_mouse-monoclonal-sox10-antibody-sox10-1074-simple-western-2842023135456..jpg)
![Western Blot: SOX10 Antibody (PDH0-03) [NBP3-32970] - Western blot analysis of SOX10 on different lysates with Rabbit anti-SOX10 antibody (NBP3-32970) at 1/2,000 dilution. Lane 1: A375 cell lysate Lane 2: B16F1 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 50 kDa Observed band size: 60-75kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32970) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32970_rabbit-sox10-mab-pdh0-03-288202412493436.jpg "Western Blot: SOX10 Antibody (PDH0-03) [NBP3-32970] - Western blot analysis of SOX10 on different lysates with Rabbit anti-SOX10 antibody (NBP3-32970) at 1/2,000 dilution. Lane 1: A375 cell lysate Lane 2: B16F1 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 50 kDa Observed band size: 60-75kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32970) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: SOX10 Antibody (PDH0-03) [NBP3-32970] - Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue with Rabbit anti-SOX10 antibody (NBP3-32970) at 1/3,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32970) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32970_rabbit-sox10-mab-pdh0-03-298202413585564.jpg)
![Immunocytochemistry/ Immunofluorescence: SOX10 Antibody (PDH0-03) [NBP3-32970] - Immunocytochemistry analysis of A375 cells labeling SOX10 with Rabbit anti-SOX10 antibody (NBP3-32970) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-SOX10 antibody (NBP3-32970) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32970_rabbit-sox10-mab-pdh0-03-2920241192369.jpg)
![Flow Cytometry: SOX10 Antibody (PDH0-03) [NBP3-32970] - Flow cytometric analysis of A375 cells labeling SOX10. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32970, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32970_rabbit-sox10-mab-pdh0-03-39202488465.jpg)
![Western Blot: SOX10 Antibody (PDH0-05) [NBP3-32969] - Western blot analysis of SOX10 on different lysates with Rabbit anti-SOX10 antibody (NBP3-32969) at 1/500 dilution. Lane 1: A375 cell lysate Lane 2: B16F1 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 50 kDa Observed band size: 60-75kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32969) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32969_rabbit-sox10-mab-pdh0-05-288202412493490.jpg "Western Blot: SOX10 Antibody (PDH0-05) [NBP3-32969] - Western blot analysis of SOX10 on different lysates with Rabbit anti-SOX10 antibody (NBP3-32969) at 1/500 dilution. Lane 1: A375 cell lysate Lane 2: B16F1 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 50 kDa Observed band size: 60-75kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32969) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: SOX10 Antibody (PDH0-05) [NBP3-32969] - Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue with Rabbit anti-SOX10 antibody (NBP3-32969) at 1/3,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32969) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32969_rabbit-sox10-mab-pdh0-05-29820241420929.jpg)
![Immunocytochemistry/ Immunofluorescence: SOX10 Antibody (PDH0-05) [NBP3-32969] - Immunocytochemistry analysis of A375 cells labeling SOX10 with Rabbit anti-SOX10 antibody (NBP3-32969) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-SOX10 antibody (NBP3-32969) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32969_rabbit-sox10-mab-pdh0-05-29202410334033.jpg)
![Flow Cytometry: SOX10 Antibody (PDH0-05) [NBP3-32969] - Flow cytometric analysis of A375 cells labeling SOX10. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32969, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32969_rabbit-sox10-mab-pdh0-05-392024884658.jpg)
![Western Blot: SOX10 Antibody (PDH0-04) [NBP3-32971] - Western blot analysis of SOX10 on different lysates with Rabbit anti-SOX10 antibody (NBP3-32971) at 1/2,000 dilution. Lane 1: A375 cell lysate Lane 2: B16F1 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 50 kDa Observed band size: 60-75kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32971) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32971_rabbit-sox10-mab-pdh0-04-288202412493488.jpg "Western Blot: SOX10 Antibody (PDH0-04) [NBP3-32971] - Western blot analysis of SOX10 on different lysates with Rabbit anti-SOX10 antibody (NBP3-32971) at 1/2,000 dilution. Lane 1: A375 cell lysate Lane 2: B16F1 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 50 kDa Observed band size: 60-75kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32971) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: SOX10 Antibody (PDH0-04) [NBP3-32971] - Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue with Rabbit anti-SOX10 antibody (NBP3-32971) at 1/3,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32971) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32971_rabbit-sox10-mab-pdh0-04-298202414333699.jpg)
![Immunocytochemistry/ Immunofluorescence: SOX10 Antibody (PDH0-04) [NBP3-32971] - Immunocytochemistry analysis of A375 cells labeling SOX10 with Rabbit anti-SOX10 antibody (NBP3-32971) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-SOX10 antibody (NBP3-32971) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32971_rabbit-sox10-mab-pdh0-04-2920241192371.jpg)
![Flow Cytometry: SOX10 Antibody (PDH0-04) [NBP3-32971] - Flow cytometric analysis of A375 cells labeling SOX10. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32971, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32971_rabbit-sox10-mab-pdh0-04-392024884668.jpg)
![Western Blot: SOX10 Antibody (SPM607) [NBP2-44475] - Western Blot Analysis of COLO-38 using SOX10 MAb (SPM607).](http://images.novusbio.com/fullsize/SOX10-Antibody-SPM607-Western-Blot-NBP2-44475-img0003.jpg "Western Blot: SOX10 Antibody (SPM607) [NBP2-44475] - Western Blot Analysis of COLO-38 using SOX10 MAb (SPM607).")
![Immunohistochemistry-Paraffin: SOX10 Antibody (SPM607) [NBP2-44475] - Human Melanoma stained with SOX10 Monoclonal Antibody (SPM607).](http://images.novusbio.com/fullsize/SOX10-Antibody-SPM607-Immunohistochemistry-Paraffin-NBP2-44475-img0001.jpg)
