PTEN Antibodies
We offer PTEN Antibodies for use in common research applications: ELISA, Flow (Intracellular), Flow Cytometry, Immunocytochemistry, Immunocytochemistry/Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Intracellular Staining by Flow Cytometry, Peptide ELISA, Proximity Ligation Assay, Sandwich ELISA, Simple Western, Western Blot. Each PTEN Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our PTEN Antibodies can be used in a variety of model species: Bovine, Chicken, Human, Mouse, Rat. Use the list below to choose the PTEN Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our PTEN polyclonal antibodies and browse our PTEN monoclonal antibody catalog.
Alternate Names for PTEN Antibodies
anti-PTEN antibody, anti-BZS antibody, anti-EC 3.1.3.16,10q23del antibody, anti-EC 3.1.3.48 antibody, anti-EC 3.1.3.67 antibody, anti-GLM2 antibody, anti-MMAC1 phosphatase and tensin homolog deleted on chromosome 10 antibody, anti-MMAC1MGC11227 antibody, anti-Mutated in multiple advanced cancers 1 antibody, anti-phosphatase and tensin homologDEC antibody, anti-phosphatidylinositol-34,5-trisphosphate 3-phosphatase and dual-specificityprotein phosphatase PTEN antibody, anti-PTEN1 antibody, anti-TEP1MHAM antibody
22 Results for "pten" in Primary Antibodies
![Western Blot: PTEN Antibody (7O6F4) [NBP3-15666] - Analysis of extracts of C6 cells, using PTEN antibody (NBP3-15666) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/PTEN-Antibody-7O6F4-Western-Blot-NBP3-15666-img0005.jpg "Western Blot: PTEN Antibody (7O6F4) [NBP3-15666] - Analysis of extracts of C6 cells, using PTEN antibody (NBP3-15666) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Immunocytochemistry/Immunofluorescence: PTEN Antibody (7O6F4) [NBP3-15666] - Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] PTEN Rabbit mAb (NBP3-15666) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/PTEN-Antibody-7O6F4-Immunocytochemistry-Immunofluorescence-NBP3-15666-img0003.jpg)
![Immunohistochemistry-Paraffin: PTEN Antibody (7O6F4) [NBP3-15666] - Human breast cancer using [KO Validated] PTEN Rabbit mAb (NBP3-15666) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PTEN-Antibody-7O6F4-Immunohistochemistry-Paraffin-NBP3-15666-img0002.jpg)
![Western Blot: PTEN Antibody (7O6F4) [NBP3-15666] - Analysis of extracts of various cell lines, using PTEN antibody (NBP3-15666) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/PTEN-Antibody-7O6F4-Western-Blot-NBP3-15666-img0004.jpg)
![<b>Genetic Strategies Validation. </b>Knockout Validated: PTEN Antibody (7O6F4) [NBP3-15666] - Analysis of extracts from normal (control) and PTEN knockout (KO) HeLa cells, using PTEN antibody (NBP3-15666) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/PTEN-Antibody-7O6F4-Knockout-Validated-NBP3-15666-img0001.jpg)
![Immunoprecipitation: PTEN Antibody (7O6F4) [NBP3-15666] - Analysis of 300ug extracts of NIH/3T3 cells using 3ug PTEN antibody (NBP3-15666). Western blot was performed from the immunoprecipitate using PTEN antibody (NBP3-15666) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/PTEN-Antibody-7O6F4-Immunoprecipitation-NBP3-15666-img0006.jpg)
![Western Blot: PTEN [p Thr366, p Ser370] Antibody [NBP3-12919] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PTEN (phospho Thr366/Ser370) antibody (NBP3-12919) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PTEN-[p-Thr366--p-Ser370]-Antibody-Western-Blot-NBP3-12919-img0004.jpg "Western Blot: PTEN [p Thr366, p Ser370] Antibody [NBP3-12919] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PTEN (phospho Thr366/Ser370) antibody (NBP3-12919) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Western Blot: PTEN [p Thr366, p Ser370] Antibody [NBP3-12919] - Untreated (-) and treated (+) A431 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PTEN (phospho Thr366/pS370) antibody (NBP3-12919) diluted at 1:500. (CIP: Calf-intestinal alkaline phosphatase)](http://images.novusbio.com/fullsize/PTEN-[p-Thr366--p-Ser370]-Antibody-Western-Blot-NBP3-12919-img0001.jpg)
![Western Blot: PTEN [p Thr366, p Ser370] Antibody [NBP3-12919] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PTEN (phospho Thr366/Ser370) antibody (NBP3-12919) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PTEN-[p-Thr366--p-Ser370]-Antibody-Western-Blot-NBP3-12919-img0003.jpg)
![Western Blot: PTEN [p Thr366, p Ser370] Antibody [NBP3-12919] - Untreated (-) and treated (+) A431 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PTEN antibody (NBP3-12919) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-12919_rabbit-pten-p-thr-366-p-ser-370-pab-9102024204126.jpg)
![Immunocytochemistry/ Immunofluorescence: PTEN [p Thr366, p Ser370] Antibody [NBP3-12919] - PTEN antibody detects PTEN protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: PTEN stained by PTEN antibody (NBP3-12919) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .Scale bar= 10um.](http://images.novusbio.com/fullsize/nbp3-12919_rabbit-pten-p-thr-366-p-ser-370-pab-91020242001095.jpg)
![Western Blot: PTEN Antibody [NB100-2229] - Detection of Human and Mouse PTEN on HeLa whole cell lysate using NB100-2229. PTEN was also IPed by rabbit anti-PTEN antibodies NB100-2227, NB100-2228 and another PTEN antibody.](http://images.novusbio.com/fullsize/PTEN-Antibody-Western-Blot-NB100-2229-img0001.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: PTEN Antibody [NB100-2229] - Detection of Human and Mouse PTEN on HeLa whole cell lysate using NB100-2229. PTEN was also IPed by rabbit anti-PTEN antibodies NB100-2227, NB100-2228 and another PTEN antibody.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry-Paraffin: PTEN Antibody [NB100-2229] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti- PTEN used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/PTEN-Antibody-Immunohistochemistry-NB100-2229-img0008.jpg)
![Immunohistochemistry: PTEN Antibody (SJ19-03) [NBP3-32872] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-PTEN antibody (NBP3-32872) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32872) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32872_rabbit-pten-mab-sj19-03-29820241492640.jpg "Immunohistochemistry: PTEN Antibody (SJ19-03) [NBP3-32872] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-PTEN antibody (NBP3-32872) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32872) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: PTEN Antibody (SJ19-03) [NBP3-32872] - ICC staining of PTEN in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (NBP3-32872, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32872_rabbit-pten-mab-sj19-03-2920241192394.jpg)
![Western Blot: PTEN Antibody (SJ19-03) [NBP3-32872] - Western blot analysis of PTEN on different lysates with Rabbit anti-PTEN antibody (NBP3-32872) at 1/2,000 dilution. Lane 1: MCF7 cell lysate Lane 2: MDA-MB-468 cell lysate (negative) Lane 3: A431 cell lysate Lane 4: HeLa cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: C6 cell lysate Lane 7: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 47 kDa Observed band size: 55 kDa Exposure time: 1 minute 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32872_rabbit-pten-mab-sj19-03-3920248453545.jpg)
![Western Blot: PTEN Antibody (JE51-96) [NBP3-32873] - Western blot analysis of PTEN on different lysates with Rabbit anti-PTEN antibody (NBP3-32873) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: MCF7 cell lysate Lane 3: MDA-MB-468 cell lysate (negative) Lane 4: A431 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: C6 cell lysate Lane 7: PC-12 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 47 kDa Observed band size: 55 kDa Exposure time: 2 minutes 18 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32873) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32873_rabbit-pten-mab-je51-96-288202412461629.jpg "Western Blot: PTEN Antibody (JE51-96) [NBP3-32873] - Western blot analysis of PTEN on different lysates with Rabbit anti-PTEN antibody (NBP3-32873) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: MCF7 cell lysate Lane 3: MDA-MB-468 cell lysate (negative) Lane 4: A431 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: C6 cell lysate Lane 7: PC-12 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 47 kDa Observed band size: 55 kDa Exposure time: 2 minutes 18 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32873) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PTEN Antibody (JE51-96) [NBP3-32873] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-PTEN antibody (NBP3-32873) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32873) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32873_rabbit-pten-mab-je51-96-29820241492644.jpg)
![Flow Cytometry: PTEN Antibody (JE51-96) [NBP3-32873] - Flow cytometric analysis of A431 cells labeling PTEN. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32873, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32873_rabbit-pten-mab-je51-96-29202416372137.jpg)