PTBP1 Antibodies
We offer PTBP1 Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Peptide ELISA, Sandwich ELISA, Western Blot. Each PTBP1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our PTBP1 Antibodies can be used in a variety of model species: Human, Mouse, Porcine. Use the list below to choose the PTBP1 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our PTBP1 polyclonal antibodies and browse our PTBP1 monoclonal antibody catalog.
Alternate Names for PTBP1 Antibodies
anti-PTBP1 antibody, anti-Heterogeneous nuclear ribonucleoprotein I antibody, anti-heterogeneous nuclear ribonucleoprotein polypeptide I antibody, anti-hnRNP I antibody, anti-HNRNP-I antibody, anti-HNRNPI antibody, anti-HNRPI antibody, anti-MGC10830 antibody, anti-MGC8461 antibody, anti-polypyrimidine tract binding protein (heterogeneous nuclear ribonucleoproteinI) antibody, anti-polypyrimidine tract binding protein 1 antibody, anti-polypyrimidine tract-binding protein 1 antibody, anti-pPTB antibody, anti-PTB-1 antibody, anti-PTB-T antibody, anti-PTB2 antibody, anti-PTB3 antibody, anti-PTB4 antibody, anti-PTB57 kDa RNA-binding protein PPTB-1 antibody, anti-RNA-binding protein antibody
11 Results for "ptbp1" in Primary Antibodies
![Western Blot: PTBP1 Antibody (3H8) [H00005725-M01] - Analysis of PTBP1 expression in transfected 293T cell line by PTBP1 monoclonal antibody (M01), clone 3H8.Lane 1: PTBP1 transfected lysate(59.6 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/PTBP1-Antibody-3H8-Western-Blot-H00005725-M01-img0019.jpg "Western Blot: PTBP1 Antibody (3H8) [H00005725-M01] - Analysis of PTBP1 expression in transfected 293T cell line by PTBP1 monoclonal antibody (M01), clone 3H8.Lane 1: PTBP1 transfected lysate(59.6 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: PTBP1 Antibody (3H8) [H00005725-M01] - Analysis of monoclonal antibody to PTBP1 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/PTBP1-Antibody-3H8-Immunocytochemistry-Immunofluorescence-H00005725-M01-img0013.jpg)
![Immunohistochemistry-Paraffin: PTBP1 Antibody (3H8) [H00005725-M01] - Analysis of monoclonal antibody to PTBP1 on formalin-fixed paraffin-embedded human kidney. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/PTBP1-Antibody-3H8-Immunohistochemistry-Paraffin-H00005725-M01-img0015.jpg)
![Western Blot: PTBP1 Antibody (3H8) [H00005725-M01] - 2 minute exposure for Mouse embryonic stem cell - V6.5 with a dilution of 1:500 - Courtesy of Paul Fields at MIT](http://images.novusbio.com/fullsize/PTBP1-Antibody-3H8-Western-Blot-H00005725-M01-img0008.jpg)
![Western Blot: PTBP1 Antibody (3H8) [H00005725-M01] - 5 minute exposure for Mouse embryonic stem cell - V6.5 with a dilution of 1:500 - Courtesy of Paul Fields at MIT](http://images.novusbio.com/fullsize/PTBP1-Antibody-3H8-Western-Blot-H00005725-M01-img0009.jpg)
![Western Blot: PTBP1 Antibody (3H8) [H00005725-M01] - PTBP1 monoclonal antibody (M01), clone 3H8 Analysis of PTBP1 expression in A-431.](http://images.novusbio.com/fullsize/PTBP1-Antibody-3H8-Western-Blot-H00005725-M01-img0018.jpg)
![Immunoprecipitation: PTBP1 Antibody (3H8) [H00005725-M01] - Analysis of PTBP1 transfected lysate using anti-PTBP1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PTBP1 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/PTBP1-Antibody-3H8-Immunoprecipitation-H00005725-M01-img0016.jpg)
![ELISA: PTBP1 Antibody (3H8) [H00005725-M01] - Detection limit for recombinant GST tagged PTBP1 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PTBP1-Antibody-3H8-ELISA-H00005725-M01-img0020.jpg)
![Western Blot: PTBP1 Antibody [NB100-1310] - (0.03ug/ml) staining of lysates of cell line NIH3T3 (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/PTBP1-Antibody-Western-Blot-NB100-1310-img0009.jpg "Western Blot: PTBP1 Antibody [NB100-1310] - (0.03ug/ml) staining of lysates of cell line NIH3T3 (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: PTBP1 Antibody [NB100-1310] - (2ug/ml) Human Kidney. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.](http://images.novusbio.com/fullsize/PTBP1-Antibody-Immunohistochemistry-Paraffin-NB100-1310-img0006.jpg)
![Western Blot: PTBP1 Antibody [NB100-1310] - (0.1ug/ml) staining of Human Ovary lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/PTBP1-Antibody-Western-Blot-NB100-1310-img0007.jpg)
![Western Blot: PTBP1 Antibody [NB100-1310] - (0.01ug/ml) staining of HeLa (A), HepG2 (B), Jurkat (C) and HEK293 (D) nuclear lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/PTBP1-Antibody-Western-Blot-NB100-1310-img0008.jpg)
![Western Blot: PTBP1 Antibody [NB100-1310] - (0.1-0.3ug/ml) staining of Mouse Ovary (A) and Rat Ovary (B) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/PTBP1-Antibody-Western-Blot-NB100-1310-img0010.jpg)
![Western Blot: PTBP1 Antibody (9M6A8) [NBP3-16197] - Western blot analysis of extracts of various cell lines, using PTBP1 Rabbit mAb (NBP3-16197) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/PTBP1-Antibody-9M6A8-Western-Blot-NBP3-16197-img0004.jpg "Western Blot: PTBP1 Antibody (9M6A8) [NBP3-16197] - Western blot analysis of extracts of various cell lines, using PTBP1 Rabbit mAb (NBP3-16197) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/Immunofluorescence: PTBP1 Antibody (9M6A8) [NBP3-16197] - Immunofluorescence analysis of U-2 OS cells using PTBP1 Rabbit mAb (NBP3-16197) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/PTBP1-Antibody-9M6A8-Immunocytochemistry-Immunofluorescence-NBP3-16197-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: PTBP1 Antibody (9M6A8) [NBP3-16197] - Immunofluorescence analysis of NIH-3T3 cells using PTBP1 Rabbit mAb (NBP3-16197) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/PTBP1-Antibody-9M6A8-Immunocytochemistry-Immunofluorescence-NBP3-16197-img0002.jpg)
![Immunohistochemistry: PTBP1 Antibody (9M6A8) [NBP3-16197] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using PTBP1 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16197_rabbit-ptbp1-mab-9m6a8-52202511411425.jpg)
![Immunohistochemistry: PTBP1 Antibody (9M6A8) [NBP3-16197] - Immunohistochemistry analysis of paraffin-embedded Human pancreas tissue using PTBP1 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16197_rabbit-ptbp1-mab-9m6a8-52202511361220.jpg)
![Immunohistochemistry: PTBP1 Antibody (9M6A8) [PTBP1] - Immunohistochemistry analysis of paraffin-embedded Human pancreas tissue using PTBP1 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16197_rabbit-ptbp1-mab-9m6a8-52202517223996.jpg)
![Immunohistochemistry: PTBP1 Antibody (9M6A8) [PTBP1] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using PTBP1 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16197_rabbit-ptbp1-mab-9m6a8-52202517172892.jpg)
![Western Blot: PTBP1 Antibody (5C1-R) [NBP3-32870] - Western blot analysis of PTBP1 on different lysates with Mouse anti-PTBP1 antibody (NBP3-32870) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: HepG2 cell lysate (20 ug/Lane) Lane 3: Jurkat cell lysate (20 ug/Lane) Lane 4: K-562 cell lysate (20 ug/Lane) Lane 5: 293T cell lysate (20 ug/Lane) Lane 6: SW620 cell lysate (20 ug/Lane) Lane 7: A431 cell lysate (20 ug/Lane) Lane 8: A549 cell lysate (20 ug/Lane) Lane 9: NIH/3T3 cell lysate (20 ug/Lane) Lane 10: C6 cell lysate (20 ug/Lane) Lane 11: Human kidney tissue lysate (40 ug/Lane) Predicted band size: 60 kDa Observed band size: 55 kDa Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32870) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32870_mouse-ptbp1-mab-5c1-r-288202412461666.jpg "Western Blot: PTBP1 Antibody (5C1-R) [NBP3-32870] - Western blot analysis of PTBP1 on different lysates with Mouse anti-PTBP1 antibody (NBP3-32870) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: HepG2 cell lysate (20 ug/Lane) Lane 3: Jurkat cell lysate (20 ug/Lane) Lane 4: K-562 cell lysate (20 ug/Lane) Lane 5: 293T cell lysate (20 ug/Lane) Lane 6: SW620 cell lysate (20 ug/Lane) Lane 7: A431 cell lysate (20 ug/Lane) Lane 8: A549 cell lysate (20 ug/Lane) Lane 9: NIH/3T3 cell lysate (20 ug/Lane) Lane 10: C6 cell lysate (20 ug/Lane) Lane 11: Human kidney tissue lysate (40 ug/Lane) Predicted band size: 60 kDa Observed band size: 55 kDa Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32870) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PTBP1 Antibody (5C1-R) [NBP3-32870] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Mouse anti-PTBP1 antibody (NBP3-32870) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32870) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32870_mouse-ptbp1-mab-5c1-r-29820241492634.jpg)
![Immunocytochemistry/ Immunofluorescence: PTBP1 Antibody (5C1-R) [NBP3-32870] - Immunocytochemistry analysis of HeLa cells labeling PTBP1 with Mouse anti-PTBP1 antibody (NBP3-32870) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-PTBP1 antibody (NBP3-32870) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32870_mouse-ptbp1-mab-5c1-r-2920241114831.jpg)
![Western Blot: PTBP1 Antibody [NBP2-19996] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PTBP1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PTBP1-Antibody-Western-Blot-NBP2-19996-img0007.jpg "Western Blot: PTBP1 Antibody [NBP2-19996] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PTBP1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: PTBP1 Antibody [NBP2-19996] - Mouse brain. PTBP1 stained by PTBP1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/PTBP1-Antibody-Immunohistochemistry-Paraffin-NBP2-19996-img0008.jpg)
![Western Blot: PTBP1 Antibody [NBP2-19996] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PTBP1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PTBP1-Antibody-Western-Blot-NBP2-19996-img0005.jpg)
![Immunohistochemistry-Paraffin: PTBP1 Antibody [NBP2-19996] - AGS xenograft, using hnRNP 1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/PTBP1-Antibody-Immunohistochemistry-Paraffin-NBP2-19996-img0003.jpg)
![Immunohistochemistry: PTBP1 Antibody (8A1) [NBP3-26363] - Image of PTBP1 Antibody (8A1) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26363_rabbit-ptbp1-mab-8a1-262202415355729.jpg "Immunohistochemistry: PTBP1 Antibody (8A1) [NBP3-26363] - Image of PTBP1 Antibody (8A1) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: PTBP1 Antibody (8A1) [NBP3-26363] - Image of PTBP1 Antibody (8A1) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26363_rabbit-ptbp1-mab-8a1-2622024152244.jpg)
![Western Blot: PTBP1 Antibody (8A1) [NBP3-26363] - Positive Western Blot detected in: Hela whole cell lysate, HepG2 whole cell lysate, PC-3 whole cell lysate, K562 whole cell lysate, HL-60 whole cell lysate, MCF-7 whole cell lysate, Jurkat whole cell lysate. All lanes: PTBP1 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 58, 60, 60 kDa Observed band size: 58 kDa](http://images.novusbio.com/fullsize/nbp3-26363_rabbit-ptbp1-mab-8a1-2822024115252.jpg)
![Western Blot: PTBP1 Antibody (S01-1G2) [NBP3-19910] - Western blot detection of PTBP1 in Hela, CHO-K1, Ramos using NBP3-19910 (1:1000 diluted)](http://images.novusbio.com/fullsize/PTBP1-Antibody-S01-1G2-Western-Blot-NBP3-19910-img0002.jpg "Western Blot: PTBP1 Antibody (S01-1G2) [NBP3-19910] - Western blot detection of PTBP1 in Hela, CHO-K1, Ramos using NBP3-19910 (1:1000 diluted)")
![Immunohistochemistry-Paraffin: PTBP1 Antibody (S01-1G2) [NBP3-19910] - Immunohistochemistry of PTBP1 in paraffin-embedded Human tonsil using NBP3-19910 at dilution 1/50](http://images.novusbio.com/fullsize/PTBP1-Antibody-S01-1G2-Immunohistochemistry-Paraffin-NBP3-19910-img0001.jpg)
![Western Blot: PTBP1 Antibody [NBP3-02956] - Analysis of extracts from normal (control) and PTBP1 knockout (KO) HeLa cells, using PTBP1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit . Exposure time: 3min.](http://images.novusbio.com/fullsize/PTBP1-Antibody-Knockout-Validated-NBP3-02956-img0001.jpg "<b>Genetic Strategies Validation. </b>Western Blot: PTBP1 Antibody [NBP3-02956] - Analysis of extracts from normal (control) and PTBP1 knockout (KO) HeLa cells, using PTBP1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit . Exposure time: 3min.")
![Western Blot: PTBP1 Antibody [NBP1-57123] - Sample Tissue: Human HepG2 Antibody Dilution: 1.0 ug/ml](http://images.novusbio.com/fullsize/PTBP1-Antibody-Western-Blot-NBP1-57123-img0003.jpg "Western Blot: PTBP1 Antibody [NBP1-57123] - Sample Tissue: Human HepG2 Antibody Dilution: 1.0 ug/ml")
![Western Blot: PTBP1 Antibody [NBP1-57123] - Reccomended Titration: 0.2 - 1 ug/ml Positive Control: Daudi cell lysate PTBP1 is strongly supported by BioGPS gene expression data to be expressed in Human Daudi cells](http://images.novusbio.com/fullsize/PTBP1-Antibody-Western-Blot-NBP1-57123-img0004.jpg)
![Western Blot: PTBP1 Antibody [NBP1-57124] - Jurkat cell lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/PTBP1-Antibody-Western-Blot-NBP1-57124-img0002.jpg "Western Blot: PTBP1 Antibody [NBP1-57124] - Jurkat cell lysate, concentration 0.2-1 ug/ml.")
![Western Blot: PTBP1 Antibody [H00005725-D01P] - Analysis of PTBP1 expression in transfected 293T cell line by PTBP1 polyclonal antibody.Lane 1: PTBP1 transfected lysate(59.60 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/PTBP1-Antibody-Western-Blot-H00005725-D01P-img0002.jpg "Western Blot: PTBP1 Antibody [H00005725-D01P] - Analysis of PTBP1 expression in transfected 293T cell line by PTBP1 polyclonal antibody.Lane 1: PTBP1 transfected lysate(59.60 KDa).Lane 2: Non-transfected lysate.")