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PTBP1 Antibody

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Western Blot: PTBP1 Antibody [NB100-1310] - (0.03ug/ml) staining of lysates of cell line NIH3T3 (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: PTBP1 Antibody [NB100-1310] - (0.03ug/ml) staining of lysates of cell line NIH3T3 (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: PTBP1 Antibody [NB100-1310] - (2ug/ml) Human Kidney. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
Western Blot: PTBP1 Antibody [NB100-1310] - (0.1ug/ml) staining of Human Ovary lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: PTBP1 Antibody [NB100-1310] - (0.01ug/ml) staining of HeLa (A), HepG2 (B), Jurkat (C) and HEK293 (D) nuclear lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by ...read more
Western Blot: PTBP1 Antibody [NB100-1310] - (0.1-0.3ug/ml) staining of Mouse Ovary (A) and Rat Ovary (B) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, CaSpecies Glossary
Applications WB, ELISA, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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PTBP1 Antibody Summary

Immunogen
Peptide with sequence DGIVPDIAVGTKR-C corresponding to N-Terminus according to NP_114367.1, NP_114368.1, NP_787041.1.
Specificity
This antibody is expected to recognise all 4 reported isoforms (represented by NP_002810.1; NP_114367.1; NP_114368.1; NP_787041.1).
Predicted Species
Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PTBP1
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 2 - 4 ug/ml
  • Immunohistochemistry-Paraffin 2-4 ug/ml
  • Peptide ELISA detection Limit 1:32000
  • Western Blot 0.1-0.3 ug/ml
Application Notes
WB: Approx. 55 kDa band observed in human liver and ovary lysates, and 60 kDa band observed in nuclear lysates of HeLa, HepG2, Jurkat and HEK293 (calculated MW of 57.2 kDa band according to NP_114368.1). IHC-P: Human kidney shows nuclear membrane staining in all the convoluted tubules.
IHC: In paraffin embedded Human Kidney shows nuclear membrane staining in all the convoluted tubules
Control
Jurkat Whole Cell Lysate
Publications
Read Publications using NB100-1310.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PTBP1 Antibody

  • Heterogeneous nuclear ribonucleoprotein I
  • heterogeneous nuclear ribonucleoprotein polypeptide I
  • hnRNP I
  • HNRNPI
  • HNRNP-I
  • HNRPI
  • MGC10830
  • MGC8461
  • polypyrimidine tract binding protein (heterogeneous nuclear ribonucleoproteinI)
  • polypyrimidine tract binding protein 1
  • polypyrimidine tract-binding protein 1
  • pPTB
  • PTB-1
  • PTB2
  • PTB3
  • PTB4
  • PTB57 kDa RNA-binding protein PPTB-1
  • PTB-T
  • RNA-binding protein

Background

Polypyrimidine tract-binding protein (PTB), also known as heterogeneous nuclear ribonucleoprotein type I (hnRNP I), is a 59.6-kD nuclear protein that binds pre-mRNAs in specific regions of the hnRNA-protein complexes sensitive to micrococcal nuclease. The hnRNP I protein shows an unusual pattern of nuclear localization. It has been implicated in pre-mRNA splicing.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PTBP1 Antibody (NB100-1310)(2)


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Product General Protocols

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Video Protocols

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Control Lysate(s)

Jurkat Whole Cell Lysate

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PTBP1
Entrez
Uniprot