EBP1 Antibodies
We offer PA2G4/EBP1 Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Sandwich ELISA, Western Blot. Each PA2G4/EBP1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our PA2G4/EBP1 Antibodies can be used in a variety of model species: Human, Mouse. Use the list below to choose the PA2G4/EBP1 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our PA2G4/EBP1 polyclonal antibodies and browse our PA2G4/EBP1 monoclonal antibody catalog.
Alternate Names for EBP1 Antibodies
anti-PA2G4/EBP1 antibody, anti-PA2G4 antibody, anti-Cell cycle protein p38-2G4 homolog antibody, anti-EBP1 antibody, anti-ErbB-3 binding protein 1 antibody, anti-ErbB3-binding protein 1 antibody, anti-ErbB3-binding protein Ebp1 antibody, anti-hG4-1 antibody, anti-p38-2G4 antibody, anti-proliferation-associated 2G4, 38kD antibody, anti-proliferation-associated 2G4, 38kDa antibody, anti-proliferation-associated protein 2G4 antibody
14 Results for "pa2g4-ebp1" in Primary Antibodies
![Western Blot: PA2G4/EBP1 Antibody (9J9U5) [NBP3-16561] - Western blot analysis of extracts of various cell lines, using PA2G4/EBP1Rabbit mAb (NBP3-16561) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/PA2G4-EBP1-Antibody-9J9U5-Western-Blot-NBP3-16561-img0005.jpg "Western Blot: PA2G4/EBP1 Antibody (9J9U5) [NBP3-16561] - Western blot analysis of extracts of various cell lines, using PA2G4/EBP1Rabbit mAb (NBP3-16561) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.")
![Immunocytochemistry/Immunofluorescence: PA2G4/EBP1 Antibody (9J9U5) [NBP3-16561] - Immunofluorescence analysis of NIH-3T3 cells using PA2G4/EBP1Rabbit mAb (NBP3-16561) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/PA2G4-EBP1-Antibody-9J9U5-Immunocytochemistry-Immunofluorescence-NBP3-16561-img0004.jpg)
![Immunohistochemistry-Paraffin: PA2G4/EBP1 Antibody (9J9U5) [NBP3-16561] - Immunohistochemistry of paraffin-embedded mouse kidney using PA2G4/EBP1Rabbit mAb (NBP3-16561) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PA2G4-EBP1-Antibody-9J9U5-Immunohistochemistry-Paraffin-NBP3-16561-img0003.jpg)
![Western Blot: PA2G4/EBP1 Antibody (9J9U5) [NBP3-16561] - Western blot analysis of extracts of Rat brain, using PA2G4/EBP1Rabbit mAb (NBP3-16561) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/PA2G4-EBP1-Antibody-9J9U5-Western-Blot-NBP3-16561-img0006.jpg)
![Immunohistochemistry-Paraffin: PA2G4/EBP1 Antibody (9J9U5) [NBP3-16561] - Immunohistochemistry of paraffin-embedded rat testis using PA2G4/EBP1Rabbit mAb (NBP3-16561) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PA2G4-EBP1-Antibody-9J9U5-Immunohistochemistry-Paraffin-NBP3-16561-img0001.jpg)
![Immunohistochemistry-Paraffin: PA2G4/EBP1 Antibody (9J9U5) [NBP3-16561] - Immunohistochemistry of paraffin-embedded human colon carcinoma using PA2G4/EBP1Rabbit mAb (NBP3-16561) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PA2G4-EBP1-Antibody-9J9U5-Immunohistochemistry-Paraffin-NBP3-16561-img0002.jpg)
![Western Blot: EBP1 Antibody [NBP2-16257] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/EBP1-Antibody-Western-Blot-NBP2-16257-img0002.jpg "Western Blot: EBP1 Antibody [NBP2-16257] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 10% SDS PAGE gel, diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: EBP1 Antibody [NBP2-16257] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/EBP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16257-img0003.jpg)
![Western Blot: EBP1 Antibody [NBP2-16257] - Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/EBP1-Antibody-Western-Blot-NBP2-16257-img0001.jpg)
![Western Blot: PA2G4/EBP1 Antibody [NBP2-16257] - Sample (30 ug of whole cell lysate) A: zebrafish eye 10% SDS PAGE NBP2-16257 diluted at 1:1000](http://images.novusbio.com/fullsize/nbp2-16257_rabbit-polyclonal-pa2g4-ebp1-antibody-91020242021926.jpg)
![Immunohistochemistry: PA2G4/EBP1 Antibody [NBP2-16257] - Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using PA2G4/EBP1 antibody [N3C3] (NBP2-16257) at 1:300 dilution.](http://images.novusbio.com/fullsize/nbp2-16257_rabbit-polyclonal-pa2g4-ebp1-antibody-9102024201579.jpg)
![Western Blot: PA2G4/EBP1 Antibody (JE35-48) [NBP3-32288] - Western blot analysis of PA2G4/EBP1 on different lysates with Rabbit anti-PA2G4/EBP1 antibody (NBP3-32288) at 1/500 dilution. Lane 1: K562 cell lysate Lane 2: HEK-293 cell lysate Lane 3: PC-12 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 44 kDa Observed band size: 44 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32288) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32288_rabbit-pa2g4-ebp1-mab-je35-48-27820241633125.png "Western Blot: PA2G4/EBP1 Antibody (JE35-48) [NBP3-32288] - Western blot analysis of PA2G4/EBP1 on different lysates with Rabbit anti-PA2G4/EBP1 antibody (NBP3-32288) at 1/500 dilution. Lane 1: K562 cell lysate Lane 2: HEK-293 cell lysate Lane 3: PC-12 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 44 kDa Observed band size: 44 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32288) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PA2G4/EBP1 Antibody (JE35-48) [NBP3-32288] - Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-PA2G4/EBP1 antibody (NBP3-32288) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32288) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32288_rabbit-pa2g4-ebp1-mab-je35-48-29820241435277.jpg)
![Immunocytochemistry/ Immunofluorescence: PA2G4/EBP1 Antibody (JE35-48) [NBP3-32288] - Immunocytochemistry analysis of 293 cells labeling PA2G4/EBP1 with Rabbit anti-PA2G4/EBP1 antibody (NBP3-32288) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-PA2G4/EBP1 antibody (NBP3-32288) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32288_rabbit-pa2g4-ebp1-mab-je35-48-2920241192382.jpg)
![Immunohistochemistry-Paraffin: PA2G4/EBP1 Antibody [NBP1-71839] - FFPE serial sections of human prostate carcinoma. Antibody: Affinity purified rabbit anti-EBP1, left image and , right image used at a dilution of 1:200 (1 ug/ml). Detection: DAB. Counterstain: hematoxylin (blue).](http://images.novusbio.com/fullsize/PA2G4-EBP1-Antibody-Immunohistochemistry-Paraffin-NBP1-71839-img0004.jpg "Immunohistochemistry-Paraffin: PA2G4/EBP1 Antibody [NBP1-71839] - FFPE serial sections of human prostate carcinoma. Antibody: Affinity purified rabbit anti-EBP1, left image and , right image used at a dilution of 1:200 (1 ug/ml). Detection: DAB. Counterstain: hematoxylin (blue).")
![PA2G4/EBP1 Antibody [NBP1-71839] - Whole cell lysate (10 ug) from HEK293T, HeLa, MCF-7, Jurkat, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-EBP1 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/antibody/nbp1-71839_rabbit-polyclonal-pa2g4-ebp1-antibody-western-blot-115202394115..jpg)
![PA2G4/EBP1 Antibody [NBP1-71839] - Whole cell lysate (10 ug) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-EBP1 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/antibody/nbp1-71839_rabbit-polyclonal-pa2g4-ebp1-antibody-western-blot-115202394246..jpg)
![Western Blot: PA2G4/EBP1 Antibody [NBP1-71840] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T, 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Affinity purified rabbit anti-EBP1 antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. EBP1 was also immunoprecipitated by rabbit anti-EBP1 antibody NBP1-71839 which recognizes an upstream epitope.](http://images.novusbio.com/fullsize/PA2G4-EBP1-Antibody-Western-Blot-NBP1-71840-img0007.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: PA2G4/EBP1 Antibody [NBP1-71840] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T, 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Affinity purified rabbit anti-EBP1 antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. EBP1 was also immunoprecipitated by rabbit anti-EBP1 antibody NBP1-71839 which recognizes an upstream epitope.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry-Paraffin: PA2G4/EBP1 Antibody [NBP1-71840] - Human prostate carcinoma. Antibody: Affinity purified rabbit anti- EBP1 used at a dilution of 1:200 (1ug/ml). Detection: DAB. Counterstain: hematoxylin (blue).](http://images.novusbio.com/fullsize/PA2G4-EBP1-Antibody-Immunohistochemistry-NBP1-71840-img0008.jpg)
![Western Blot: PA2G4/EBP1 Antibody (SR1830) [NBP3-22164] - Western blot analysis using NBP3-22164 in A431 cell lysate.](http://images.novusbio.com/fullsize/nbp3-22164_rabbit-pa2g4-ebp1-mab-sr1830-57202316112113.jpg "Western Blot: PA2G4/EBP1 Antibody (SR1830) [NBP3-22164] - Western blot analysis using NBP3-22164 in A431 cell lysate.")
![Western Blot: PA2G4/EBP1 Antibody (2A5) [H00005036-M01] - Analysis of PA2G4 expression in transfected 293T cell line by PA2G4 monoclonal antibody (M01), clone 2A5.Lane 1: PA2G4 transfected lysate(43.8 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/PA2G4-EBP1-Antibody-2A5-Western-Blot-H00005036-M01-img0004.jpg "Western Blot: PA2G4/EBP1 Antibody (2A5) [H00005036-M01] - Analysis of PA2G4 expression in transfected 293T cell line by PA2G4 monoclonal antibody (M01), clone 2A5.Lane 1: PA2G4 transfected lysate(43.8 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: PA2G4/EBP1 Antibody (2A5) [H00005036-M01] - Analysis of monoclonal antibody to PA2G4 on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/PA2G4-EBP1-Antibody-2A5-Immunocytochemistry-Immunofluorescence-H00005036-M01-img0002.jpg)
![Sandwich ELISA: PA2G4/EBP1 Antibody (2A5) [H00005036-M01] - Detection limit for recombinant GST tagged PA2G4 is 1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PA2G4-EBP1-Antibody-2A5-Sandwich-ELISA-H00005036-M01-img0003.jpg)
![Western Blot: EBP1 Antibody [NBP1-90369] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/EBP1-Antibody-Western-Blot-NBP1-90369-img0004.jpg "Western Blot: EBP1 Antibody [NBP1-90369] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp")
![Immunohistochemistry-Paraffin: EBP1 Antibody [NBP1-90369] - Staining of human duodenum shows cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/EBP1-Antibody-Immunohistochemistry-Paraffin-NBP1-90369-img0001.jpg)
![Western Blot: PA2G4/EBP1 Antibody (5E7) [NBP2-59468] - Lane 1: HeLa cell lysates, Lane 2: MCF7 cell lysates, Lane 3: A549 cell lysates, Lane 4: 293T cell lysates, Lane 5: A431 cell lysates, Lane 6: Jurkat cell lysates](http://images.novusbio.com/fullsize/PA2G4-EBP1-Antibody-5E7-Western-Blot-NBP2-59468-img0001.jpg "Western Blot: PA2G4/EBP1 Antibody (5E7) [NBP2-59468] - Lane 1: HeLa cell lysates, Lane 2: MCF7 cell lysates, Lane 3: A549 cell lysates, Lane 4: 293T cell lysates, Lane 5: A431 cell lysates, Lane 6: Jurkat cell lysates")
![Immunocytochemistry/Immunofluorescence: PA2G4/EBP1 Antibody (5E7) [NBP2-59468] - Analysis of PA2G4 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PA2G4 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). Analysis of PA2G4 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PA2G4 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/PA2G4-EBP1-Antibody-5E7-Immunocytochemistry-Immunofluorescence-NBP2-59468-img0002.jpg)
![Western Blot: EBP1 Antibody [NBP2-16256] - Sample (30 ug of whole cell lysate) A: A431 B: Jurkat 10% SDS PAGE gel, diluted at 1:10000.](http://images.novusbio.com/fullsize/EBP1-Antibody-Western-Blot-NBP2-16256-img0001.jpg "Western Blot: EBP1 Antibody [NBP2-16256] - Sample (30 ug of whole cell lysate) A: A431 B: Jurkat 10% SDS PAGE gel, diluted at 1:10000.")
![Immunoprecipitation: PA2G4/EBP1 Antibody [NBP2-16256] - EBP1 antibody immunoprecipitates EBP1 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. 40 ug 293T whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of EBP1 protein by 2 ug of EBP1 antibody 7.5% SDS-PAGE. The immunoprecipitated EBP1 protein was detected by EBP1 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/PA2G4-EBP1-Antibody-Immunoprecipitation-NBP2-16256-img0003.jpg)
![Western Blot: EBP1 Antibody (1D3) [NBP2-00981] HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EBP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EBP1.](http://images.novusbio.com/fullsize/EBP1-Antibody-1D3-Western-Blot-NBP2-00981-img0001.jpg "Western Blot: EBP1 Antibody (1D3) [NBP2-00981] HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EBP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EBP1.")