NSFL1C Antibodies
We offer NSFL1C Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot. Each NSFL1C Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our NSFL1C Antibodies can be used in a variety of model species: Human, Mouse. Use the list below to choose the NSFL1C Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our NSFL1C polyclonal antibodies.
Alternate Names for NSFL1C Antibodies
anti-NSFL1C antibody, anti-dJ776F14.1 antibody, anti-NSFL1 (p97) cofactor (p47) antibody, anti-NSFL1 cofactor p47 antibody, anti-p47 antibody, anti-p97 cofactor p47 antibody, anti-SHP1 homolog antibody, anti-UBX domain protein 2C antibody, anti-UBX domain-containing protein 2C antibody, anti-UBX1 antibody, anti-UBXD10 antibody, anti-UBXN2CMGC3347 antibody
8 Results for "nsfl1c" in Primary Antibodies
![Immunohistochemistry-Paraffin: NSFL1C Antibody [NBP2-13677] - Staining of human cerebral cortex, kidney, lymph node and testis using Anti-NSFL1C antibody NBP2-13677 (A) shows similar protein distribution across tissues to independent antibody NBP2-13676 (B).](http://images.novusbio.com/fullsize/NSFL1C-Antibody-Immunohistochemistry-Paraffin-NBP2-13677-img0005.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: NSFL1C Antibody [NBP2-13677] - Staining of human cerebral cortex, kidney, lymph node and testis using Anti-NSFL1C antibody NBP2-13677 (A) shows similar protein distribution across tissues to independent antibody NBP2-13676 (B).")
![<b>Independent Antibodies Validation. </b>Western Blot: NSFL1C Antibody [NBP2-13677] - PINK1 phosphorylates the activation loop of PKA. Immunoprecipitation products of HEK293 cell lysates expressing GFP or PINK1-GFP reveal that PINK1 pulls down the PKA holoenzyme (PKAcat and PKAreg) in addition to VCP and NSFL1C. Image collected and cropped by CiteAb from the following publication (//eneuro.org/lookup/doi/10.1523/ENEURO.0466-18.2018), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NSFL1C-Antibody-Western-Blot-NBP2-13677-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: NSFL1C Antibody [NBP2-13677] - Staining of human cell line A-431 shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/NSFL1C-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-13677-img0004.jpg)
![Immunohistochemistry-Paraffin: NSFL1C Antibody [NBP2-13677] - Staining of human testis.](http://images.novusbio.com/fullsize/NSFL1C-Antibody-Immunohistochemistry-Paraffin-NBP2-13677-img0010.jpg)
![Immunohistochemistry-Paraffin: NSFL1C Antibody [NBP2-13677] - Staining of human kidney.](http://images.novusbio.com/fullsize/NSFL1C-Antibody-Immunohistochemistry-Paraffin-NBP2-13677-img0007.jpg)
![Immunohistochemistry-Paraffin: NSFL1C Antibody [NBP2-13677] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/NSFL1C-Antibody-Immunohistochemistry-Paraffin-NBP2-13677-img0008.jpg)
![Immunohistochemistry-Paraffin: NSFL1C Antibody [NBP2-13677] - Staining of human lymph node.](http://images.novusbio.com/fullsize/NSFL1C-Antibody-Immunohistochemistry-Paraffin-NBP2-13677-img0009.jpg)
![Western Blot: NSFL1C Antibody [NBP2-13677] - Analysis in human cell line A-549.](http://images.novusbio.com/fullsize/antibody/nbp2-13677_rabbit-polyclonal-nsfl1c-antibody-western-blot-3420249306..jpg)
![Western Blot: NSFL1C Antibody [NBP2-13677] - PINK1 phosphorylates the activation loop of PKA. A, IP products of HEK293 cell lysates expressing GFP or PINK1-GFP reveal that PINK1 pulls down the PKA holoenzyme (PKAcat & PKAreg) in addition to VCP & p47. B, IP products of HEK293 lysates expressing PINK1-GFP, GFP or GFP-VCP reveal that either PINK1 or VCP pull down p47, but only PINK1 pulls down PKAreg. C, IP products of HEK293 lystates expressing Vector or MYC-p47 reveals that p47 does not interact w/ PKAcat or PKAreg. D, 2-D analysis of endogenous PKA subunits in control (M14) & PINK1-3xFlag (#24) stable SH-SY5Y lines reveal an acidic shift of PKAcat (arrow), but not PKAreg, in PINK1-overexpressing cells. E, Structural model for the MD-equilibrated (100 ns) complex between hPINK1 (pink) & PKAcat (green). PKA-T197 (cyan balls) & PINK1-D362 (orange balls) are shown in relation to PINK1-bound ATP w/ two Mg2+ (purple). Red, tan, white, cyan, & blue represent O, P, H, C, & N atoms, respectively, of ATP. F, Immunoblot analysis of HEK293 cells transfected w/ GFP or PINK1-GFP reveals that PINK1-GFP increases PKAcat phosphorylation at T197, but has no effect on PKAreg phosphorylation at S99 (mean ± SD, three independent experiments, p = 0.014). G, Recombinant PKAcat purified from E. coli, which is already phosphorylated at T197, dephosphorylated by incubation w/ lambda phosphatase (PP) at 30°C × 1 h. Successful dephosphorylation verified by immunoblot. H, In vitro kinase assay of human GST-PINK1 w/ dephosphorylated recombinant PKAcat in the presence or absence of the PKA inhibitor H89 reveals that hPINK1 phosphorylates PKA at T197. I, In vitro kinase assay of GST-TcPINK1 w/ dephosphorylated recombinant PKAcat reveals that TcPINK1 is also capable of directly phosphorylating PKAcat. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30783609), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-13677_rabbit-polyclonal-nsfl1c-antibody-310202416121970.jpg)
![Western Blot: NSFL1C Antibody [NBP2-13677] - PINK1 phosphorylates the activation loop of PKA. A, IP products of HEK293 cell lysates expressing GFP or PINK1-GFP reveal that PINK1 pulls down the PKA holoenzyme (PKAcat & PKAreg) in addition to VCP & p47. B, IP products of HEK293 lysates expressing PINK1-GFP, GFP or GFP-VCP reveal that either PINK1 or VCP pull down p47, but only PINK1 pulls down PKAreg. C, IP products of HEK293 lystates expressing Vector or MYC-p47 reveals that p47 does not interact w/ PKAcat or PKAreg. D, 2-D analysis of endogenous PKA subunits in control (M14) & PINK1-3xFlag (#24) stable SH-SY5Y lines reveal an acidic shift of PKAcat (arrow), but not PKAreg, in PINK1-overexpressing cells. E, Structural model for the MD-equilibrated (100 ns) complex between hPINK1 (pink) & PKAcat (green). PKA-T197 (cyan balls) & PINK1-D362 (orange balls) are shown in relation to PINK1-bound ATP w/ two Mg2+ (purple). Red, tan, white, cyan, & blue represent O, P, H, C, & N atoms, respectively, of ATP. F, Immunoblot analysis of HEK293 cells transfected w/ GFP or PINK1-GFP reveals that PINK1-GFP increases PKAcat phosphorylation at T197, but has no effect on PKAreg phosphorylation at S99 (mean ± SD, three independent experiments, p = 0.014). G, Recombinant PKAcat purified from E. coli, which is already phosphorylated at T197, dephosphorylated by incubation w/ lambda phosphatase (PP) at 30°C × 1 h. Successful dephosphorylation verified by immunoblot. H, In vitro kinase assay of human GST-PINK1 w/ dephosphorylated recombinant PKAcat in the presence or absence of the PKA inhibitor H89 reveals that hPINK1 phosphorylates PKA at T197. I, In vitro kinase assay of GST-TcPINK1 w/ dephosphorylated recombinant PKAcat reveals that TcPINK1 is also capable of directly phosphorylating PKAcat. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30783609), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-13677_rabbit-polyclonal-nsfl1c-antibody-310202416121987.jpg)
![Western Blot: NSFL1C Antibody [NBP2-13676] - Analysis using Anti-NSFL1C antibody NBP2-13676 (A) shows similar pattern to independent antibody NBP2-13677 (B).](http://images.novusbio.com/fullsize/NSFL1C-Antibody-Western-Blot-NBP2-13676-img0006.jpg "<b>Independent Antibodies Validation. </b>Western Blot: NSFL1C Antibody [NBP2-13676] - Analysis using Anti-NSFL1C antibody NBP2-13676 (A) shows similar pattern to independent antibody NBP2-13677 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: NSFL1C Antibody [NBP2-13676] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, plasma membrane & cytosol.](http://images.novusbio.com/fullsize/NSFL1C-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-13676-img0004.jpg)
![Immunohistochemistry-Paraffin: NSFL1C Antibody [NBP2-13676] - Staining of human kidney.](http://images.novusbio.com/fullsize/NSFL1C-Antibody-Immunohistochemistry-Paraffin-NBP2-13676-img0009.jpg)
![Immunohistochemistry-Paraffin: NSFL1C Antibody [NBP2-13676] - Staining of human testis shows distinct nuclear and cytoplasmic positivity in cells in seminiferus ducts.](http://images.novusbio.com/fullsize/NSFL1C-Antibody-Immunohistochemistry-Paraffin-NBP2-13676-img0001.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: NSFL1C Antibody [NBP2-13676] - Staining of human cerebral cortex, kidney, lymph node and testis using Anti-NSFL1C antibody NBP2-13676 (A) shows similar protein distribution across tissues to independent antibody NBP2-13677 (B).](http://images.novusbio.com/fullsize/NSFL1C-Antibody-Immunohistochemistry-Paraffin-NBP2-13676-img0005.jpg)
![Immunohistochemistry-Paraffin: NSFL1C Antibody [NBP2-13676] - Staining of human testis.](http://images.novusbio.com/fullsize/NSFL1C-Antibody-Immunohistochemistry-Paraffin-NBP2-13676-img0007.jpg)
![Immunohistochemistry-Paraffin: NSFL1C Antibody [NBP2-13676] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/NSFL1C-Antibody-Immunohistochemistry-Paraffin-NBP2-13676-img0008.jpg)
![Immunohistochemistry-Paraffin: NSFL1C Antibody [NBP2-13676] - Staining of human lymph node.](http://images.novusbio.com/fullsize/NSFL1C-Antibody-Immunohistochemistry-Paraffin-NBP2-13676-img0010.jpg)
![Western Blot: NSFL1C Antibody [NBP1-31402] - Sample(30 ug whole cell lysate) A:293T B:A431 C:H1299 10% SDS PAGE, antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/NSFL1C-Antibody-Western-Blot-NBP1-31402-img0005.jpg "Western Blot: NSFL1C Antibody [NBP1-31402] - Sample(30 ug whole cell lysate) A:293T B:A431 C:H1299 10% SDS PAGE, antibody diluted at 1:1000.")
![Immunohistochemistry-Paraffin: NSFL1C Antibody [NBP1-31402] - NSFL1C antibody [N1C2] detects NSFL1C protein at cytoplasm and nucleus on mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. NSFL1C antibody [N1C2] diluted at 1:500. Antigen Retrieval: Trilogy (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/NSFL1C-Antibody-Immunohistochemistry-Paraffin-NBP1-31402-img0006.jpg)
![Western Blot: NSFL1C Antibody [NBP1-31402] - Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/NSFL1C-Antibody-Western-Blot-NBP1-31402-img0004.jpg)
![Immunohistochemistry-Paraffin: NSFL1C Antibody [NBP1-31402] - Paraffin-embedded differential N/T pair Lung SCC cancer xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/NSFL1C-Antibody-Immunohistochemistry-Paraffin-NBP1-31402-img0003.jpg)
![Immunoprecipitation: NSFL1C Antibody [NBP1-31402] - Immunoprecipitation of NSFL1C protein from A431 whole cell extracts using 5 ug of NSFL1C antibody [N1C2]. Western blot analysis was performed using NSFL1C antibody [N1C2]. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/NSFL1C-Antibody-Immunoprecipitation-NBP1-31402-img0007.jpg)
![Western Blot: NSFL1C Antibody [H00055968-B01P] - Analysis of NSFL1C expression in human placenta.](http://images.novusbio.com/fullsize/NSFL1C-Antibody-Western-Blot-H00055968-B01P-img0005.jpg "Western Blot: NSFL1C Antibody [H00055968-B01P] - Analysis of NSFL1C expression in human placenta.")
![Immunocytochemistry/Immunofluorescence: NSFL1C Antibody [H00055968-B01P] - Analysis of purified antibody to NSFL1C on HeLa cell. (antibody concentration 10 ug/ml)](http://images.novusbio.com/fullsize/NSFL1C-Antibody-Immunocytochemistry-Immunofluorescence-H00055968-B01P-img0004.jpg)
![Western Blot: NSFL1C Antibody [H00055968-B01P] - Analysis of NSFL1C expression in transfected 293T cell line by NSFL1C polyclonal antibody. Lane 1: NSFL1C transfected lysate(40.7 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/NSFL1C-Antibody-Western-Blot-H00055968-B01P-img0006.jpg)
![Immunoprecipitation: NSFL1C Antibody [NBP3-38307] - Immunoprecipitation analysis of 200 ug extracts of A-549 cells, using 3 ug NSFL1C antibody. Western blot was performed from the immunoprecipitate using NSFL1C antibody at a dilution of 1:1000.](http://images.novusbio.com/fullsize/nbp3-38307_rabbit-nsfl1c-pab-91220241410188.jpg "Immunoprecipitation: NSFL1C Antibody [NBP3-38307] - Immunoprecipitation analysis of 200 ug extracts of A-549 cells, using 3 ug NSFL1C antibody. Western blot was performed from the immunoprecipitate using NSFL1C antibody at a dilution of 1:1000.")
![Western Blot: NSFL1C Antibody [NBP3-38307] - Western blot analysis of various lysates using NSFL1C Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-38307_rabbit-nsfl1c-pab-912202414201399.jpg)
![Immunocytochemistry/ Immunofluorescence: NSFL1C Antibody [NBP3-38307] - Immunofluorescence analysis of HeLa cells using NSFL1C Rabbit pAb. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38307_rabbit-nsfl1c-pab-912202414112836.jpg)
![Western Blot: NSFL1C Antibody [H00055968-D01P] - Analysis of NSFL1C expression in transfected 293T cell line by NSFL1C polyclonal antibody.Lane 1: NSFL1C transfected lysate(40.60 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/NSFL1C-Antibody-Western-Blot-H00055968-D01P-img0001.jpg "Western Blot: NSFL1C Antibody [H00055968-D01P] - Analysis of NSFL1C expression in transfected 293T cell line by NSFL1C polyclonal antibody.Lane 1: NSFL1C transfected lysate(40.60 KDa).Lane 2: Non-transfected lysate.")
![Western Blot: NSFL1C Antibody [NBP2-36529] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NSFL1C antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/NSFL1C-Antibody-Western-Blot-NBP2-36529-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: NSFL1C Antibody [NBP2-36529] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NSFL1C antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunoprecipitation: NSFL1C Antibody [NBP2-36529] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. For blotting immunoprecipitated NSFL1C, it was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/NSFL1C-Antibody-Immunoprecipitation-NBP2-36529-img0002.jpg)