NBR1 Antibodies
We offer NBR1 Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Simple Western, Western Blot. Each NBR1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our NBR1 Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the NBR1 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our NBR1 polyclonal antibodies and browse our NBR1 monoclonal antibody catalog.
Alternate Names for NBR1 Antibodies
anti-NBR1 antibody, anti-Cell migration-inducing gene 19 protein antibody, anti-FLJ98272 antibody, anti-KIAA0049CA125 antibody, anti-M17S2FLJ55359,1A1-3B antibody, anti-Membrane component chromosome 17 surface marker 2,1A13B antibody, anti-membrane component, chromosome 17, surface marker 2 (ovarian carcinoma antigenCA125) antibody, anti-migration-inducing protein 19 antibody, anti-neighbor of BRCA1 gene 1 antibody, anti-Neighbor of BRCA1 gene 1 protein antibody, anti-next to BRCA1 gene 1 protein antibody, anti-Protein 1A1-3B antibody
6 Results for "nbr1" in Primary Antibodies
![Western Blot: NBR1 Antibody (6B11) [H00004077-M01] - Western Blot analysis of NBR1 expression in PC-12](http://images.novusbio.com/fullsize/NBR1-Antibody-6B11-Western-Blot-H00004077-M01-img0001.jpg "Western Blot: NBR1 Antibody (6B11) [H00004077-M01] - Western Blot analysis of NBR1 expression in PC-12")
![Immunocytochemistry/Immunofluorescence: NBR1 Antibody (6B11) [H00004077-M01] - Top panel: HeLa cells stably expressing EGFP-Parkin were treated with 10 uM G-TPP and fixed 8 h after treatment. Cells were stained with antibodies against the autophagy adapter protein NBR1 (red), mitochondria were counterstained with TOM20 antibodies (cyan), nuclei with Hoechst (blue). EGFP-Parkin epifluorescence is shown in green. Scale bar corresponds to 10 uM. Bottom panel: Human fibroblasts were treated with 15 uM G-TPP for 16 h and fixed and stained with antibodies against the autophagy adapter NBR (green), mitochondria were stained with antibodies against TOM20 (red), nuclei were visualized with Hoechst (blue). Scale bars indicate 10 uM. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.22287) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NBR1-Antibody-6B11-Immunocytochemistry-Immunofluorescence-H00004077-M01-img0007.jpg)
![ELISA: NBR1 Antibody (6B11) [H00004077-M01] - Detection limit for recombinant GST tagged NBR1 is approximately 0.1 ng/mL as a capture antibody.](http://images.novusbio.com/fullsize/NBR1-Antibody-6B11-ELISA-H00004077-M01-img0004.jpg)
![Simple Western: NBR1 Antibody (6B11) [H00004077-M01] - This antibody (1:25 dilution) was used to probe THP-1 macrophage lysate (concentrations shown) by Simple Western. Results from two hybridoma clones are shown. Simple Western image submitted by a verified customer review.](http://images.novusbio.com/fullsize/NBR1-Antibody-6B11-Simple-Western-H00004077-M01-img0005.jpg)
![Western Blot: NBR1 Antibody (6B11) [H00004077-M01] - G-TPP activity is conserved in primary fibroblasts(A, C) Fibroblasts were treated with 15 µM G-TPP for the indicated time points. Cells were harvested & western blots were probed with antibodies against (A) PINK1, pS65-Ub & total Ub or (C) autophagy adapter proteins. GAPDH & Vinculin served as loading control. G-TPP treatment led to PINK1 stabilization & pS65-Ub induction in primary skin fibroblasts. p62 levels were induced upon G-TPP treatment, while other adapters seemed decreased. (B, D) Human fibroblasts were treated with 15 µM G-TPP for 16 h & fixed & stained with antibodies against (B) pS65-Ub (green) or (D) the autophagy adapters NBR1, NDP52, p62, OPTN & TAX1BP1 (green). Mitochondria were stained with antibodies against TOM20 (red), nuclei were visualized with Hoechst (blue). Scale bars indicate 10 µM. A magnified image of the boxed region, the fluorescence profile along the arrow & the Pearson’s correlation coefficient of adapter protein & mitochondrial stainingare shown to the right. Shown is the mean ± SEM of at least five randomly selected images (unpaired, two-sided t-test, ***p < 0.0005). Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.22287), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00004077-m01_mouse-monoclonal-nbr1-antibody-6b11-310202415485138.jpg)
![Immunocytochemistry/ Immunofluorescence: NBR1 Antibody (6B11) [H00004077-M01] - G-TPP leads to recruitment of autophagy adapters & degradation of mitochondria(A) HeLa cells stably expressing untagged Parkin were treated with 10 µM G-TPP for 8 h. Western blots were prepared from cell lysates & probed with antibodies against LC3, phospho-TBK1 (Ser172) & TBK1. GAPDH was used as a loading control. Upon 8 h the levels of LC3-I & LC3-II were both increased. At 8 h after treatment with G-TPP but not at 4 or 24 h, TBK1 was phosphorylated. (B) HeLa cells stably expressing EGFP-Parkin were treated with 10 µM G-TPP & fixed 8 h after treatment. Cells were stained with antibodies against the autophagy adapter proteins NBR1, NDP52, OPTN, p62, & TAX1BP1 (red). Mitochondria were counterstained with TOM20 antibodies (cyan), nuclei with Hoechst (blue). EGFP-Parkin epifluorescence is shown in green. Scale bar corresponds to 10 µM. (C) HeLa cells stably expressing EGFP-Parkin & the reporter protein mitoKeima were treated with 10 µM CCCP or G-TPP & imaged over time. The ratio of ‘neutral’ mitoKeima to ‘acidic’ mitoKeima was calculated as readout for mitophagy. Parkin translocation was monitored at the same time. Values for Parkin translocation & mitophagy were normalized to 12 h treatment with 10 µM CCCP as positive control & DMSO as negative control (two-way ANOVA with Tukey’s post-hoc test, **p < 0.005, ***p < 0.0005). Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.22287), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00004077-m01_mouse-monoclonal-nbr1-antibody-6b11-310202415482269.jpg)
![Immunocytochemistry/ Immunofluorescence: NBR1 Antibody (6B11) [H00004077-M01] - G-TPP activity is conserved in primary fibroblasts(A, C) Fibroblasts were treated with 15 µM G-TPP for the indicated time points. Cells were harvested & western blots were probed with antibodies against (A) PINK1, pS65-Ub & total Ub or (C) autophagy adapter proteins. GAPDH & Vinculin served as loading control. G-TPP treatment led to PINK1 stabilization & pS65-Ub induction in primary skin fibroblasts. p62 levels were induced upon G-TPP treatment, while other adapters seemed decreased. (B, D) Human fibroblasts were treated with 15 µM G-TPP for 16 h & fixed & stained with antibodies against (B) pS65-Ub (green) or (D) the autophagy adapters NBR1, NDP52, p62, OPTN & TAX1BP1 (green). Mitochondria were stained with antibodies against TOM20 (red), nuclei were visualized with Hoechst (blue). Scale bars indicate 10 µM. A magnified image of the boxed region, the fluorescence profile along the arrow & the Pearson’s correlation coefficient of adapter protein & mitochondrial stainingare shown to the right. Shown is the mean ± SEM of at least five randomly selected images (unpaired, two-sided t-test, ***p < 0.0005). Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.22287), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00004077-m01_mouse-monoclonal-nbr1-antibody-6b11-310202415482210.jpg)
![Western Blot: NBR1 Antibody [NBP1-71703] - The antibody detects HeLa cell NBR1 (about 130 kDa). It detects a 100 kDa band in mouse brain. Western blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize/NBR1-Antibody-Western-Blot-NBP1-71703-img0011.jpg "Western Blot: NBR1 Antibody [NBP1-71703] - The antibody detects HeLa cell NBR1 (about 130 kDa). It detects a 100 kDa band in mouse brain. Western blot image submitted by a verified customer review.")
![Immunocytochemistry/Immunofluorescence: NBR1 Antibody [NBP1-71703] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with NBR1 Antibody (NBP1-71703) at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/NBR1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-71703-img0012.jpg)
![Immunohistochemistry: NBR1 Antibody [NBP1-71703] - Staining of NBRI in mouse prostate.](http://images.novusbio.com/fullsize/NBR1-Antibody-Immunohistochemistry-NBP1-71703-img0006.jpg)
![Western Blot: NBR1 Antibody [NBP1-71703] - WB analysis of NBR1 in HepG2 cell lysates.](http://images.novusbio.com/fullsize/NBR1-Antibody-Western-Blot-NBP1-71703-img0007.jpg)
![Western Blot: NBR1 Antibody [NBP1-71703] - WB analysis of 30 ug whole cell lysate of HeLa cells using NBR1 antibody.](http://images.novusbio.com/fullsize/NBR1-Antibody-Western-Blot-NBP1-71703-img0010.jpg)
![Simple Western: NBR1 Antibody [NBP1-71703] - Simple Western lane view shows a specific band for NBR1 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/NBR1-Antibody-Simple-Western-NBP1-71703-img0008.jpg)
![Western Blot: NBR1 Antibody [NBP1-89399] - Analysis in human testis tissue.](http://images.novusbio.com/fullsize/NBR1-Antibody-Western-Blot-NBP1-89399-img0005.jpg "Western Blot: NBR1 Antibody [NBP1-89399] - Analysis in human testis tissue.")
![Immunohistochemistry: NBR1 Antibody [NBP1-89399] - Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/NBR1-Antibody-Immunohistochemistry-NBP1-89399-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: NBR1 Antibody [NBP2-58516] - Staining of human cell line A549 shows localization to nucleoplasm & vesicles.](http://images.novusbio.com/fullsize/NBR1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58516-img0001.jpg "Immunocytochemistry/Immunofluorescence: NBR1 Antibody [NBP2-58516] - Staining of human cell line A549 shows localization to nucleoplasm & vesicles.")
![Immunohistochemistry: NBR1 Antibody [NBP1-89400] - Staining of human cerebral cortex shows strong cytoplasmic positivity in glial cells.](http://images.novusbio.com/fullsize/NBR1-Antibody-Immunohistochemistry-NBP1-89400-img0003.jpg "Immunohistochemistry: NBR1 Antibody [NBP1-89400] - Staining of human cerebral cortex shows strong cytoplasmic positivity in glial cells.")