MMP-12 Antibodies
We offer MMP-12 Antibodies for use in common research applications: Dot Blot, ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Peptide ELISA, Western Blot. Each MMP-12 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our MMP-12 Antibodies can be used in a variety of model species: Canine, Human, Mouse, Rat. Use the list below to choose the MMP-12 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our MMP-12 polyclonal antibodies and browse our MMP-12 monoclonal antibody catalog.
Alternate Names for MMP-12 Antibodies
anti-MMP-12 antibody, anti-MMP12 antibody, anti-EC 3.4.24 antibody, anti-hME antibody, anti-HMEEC 3.4.24.65 antibody, anti-Macrophage elastase antibody, anti-macrophage metalloelastase antibody, anti-matrix metallopeptidase 12 (macrophage elastase) antibody, anti-matrix metalloproteinase 12 (macrophage elastase) antibody, anti-Matrix metalloproteinase-12 antibody, anti-ME antibody, anti-MGC138506 antibody, anti-MME antibody
12 Results for "mmp-12" in Primary Antibodies
![Western Blot: MMP-12 Antibody (SR03-23) [NBP2-67344] - Analysis of MMP-12 on different lysates with Rabbit anti-MMP-12 antibody at 1/500 dilution. Lane 1: Mouse lung tissue lysate Lane 2: Rat lung tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 54 kDa Observed band size: 40 kDa Exposure time: 1 minute; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/MMP-12-Antibody-SR03-23-Western-Blot-NBP2-67344-img0005.jpg "Western Blot: MMP-12 Antibody (SR03-23) [NBP2-67344] - Analysis of MMP-12 on different lysates with Rabbit anti-MMP-12 antibody at 1/500 dilution. Lane 1: Mouse lung tissue lysate Lane 2: Rat lung tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 54 kDa Observed band size: 40 kDa Exposure time: 1 minute; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry-Paraffin: MMP-12 Antibody (SR03-23) [NBP2-67344] - Analysis of paraffin-embedded mouse lung tissue using anti-MMP12 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/MMP-12-Antibody-SR03-23-Immunohistochemistry-Paraffin-NBP2-67344-img0007.jpg)
![Flow Cytometry: MMP-12 Antibody (SR03-23) [NBP2-67344] - Analysis of A549 cells with MMP12 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/MMP-12-Antibody-SR03-23-Flow-Cytometry-NBP2-67344-img0001.jpg)
![Western Blot: MMP-12 Antibody (SR03-23) [NBP2-67344] - Analysis of MMP12 on different cell lysates using anti-MMP12 antibody at 1/1,000 dilution. Positive control: Lane 1: A549 Lane 2: Hela Lane 3: MCF-7 Lane 4: THP-1](http://images.novusbio.com/fullsize/MMP-12-Antibody-SR03-23-Western-Blot-NBP2-67344-img0004.jpg)
![Immunohistochemistry-Paraffin: MMP-12 Antibody (SR03-23) [NBP2-67344] - Analysis of paraffin-embedded human breast carcinomna tissue using anti-MMP12 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/MMP-12-Antibody-SR03-23-Immunohistochemistry-Paraffin-NBP2-67344-img0002.jpg)
![Immunohistochemistry-Paraffin: MMP-12 Antibody (SR03-23) [NBP2-67344] - Analysis of paraffin-embedded mouse lung tissue using anti-MMP12 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/MMP-12-Antibody-SR03-23-Immunohistochemistry-Paraffin-NBP2-67344-img0003.jpg)
![Immunohistochemistry-Paraffin: MMP-12 Antibody (SR03-23) [NBP2-67344] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-MMP-12 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/MMP-12-Antibody-SR03-23-Immunohistochemistry-Paraffin-NBP2-67344-img0006.jpg)
![Immunohistochemistry-Paraffin: Rabbit Monoclonal MMP-12 Antibody (SR03-23) [NBP2-67344] - Analysis of MMP-12 antibody on human breast cancer tissue. Pretreatment: ER2 100C 20min, Blocking: 2.5% Normal horse serum 10min, Primary Ab: 1/200 60min, HRP for Rabbit 30min, DAB 5min, Hematoxylin counterstain. Image from a verified customer review.](http://images.novusbio.com/fullsize/nbp2-67344_rabbit-monoclonal-mmp-12-antibody-sr03-23-immunohistochemistry-paraffin-1102024134142..jpg)
![Western Blot: MMP-12 Antibody [NBP1-31225] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with MMP12 antibody [N3C1], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/MMP-12-Antibody-Western-Blot-NBP1-31225-img0015.jpg "Western Blot: MMP-12 Antibody [NBP1-31225] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with MMP12 antibody [N3C1], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: MMP-12 Antibody [NBP1-31225] - Analysis of methanol-fixed H1299, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/MMP-12-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31225-img0007.jpg)
![Immunohistochemistry-Paraffin: MMP-12 Antibody [NBP1-31225] - Imaging of Human breast cancer tissue. MMP12 is stained by antibody (green) and nucleus is stained with DAPI (blue). Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/MMP-12-Antibody-Immunohistochemistry-Paraffin-NBP1-31225-img0013.jpg)
![Immunohistochemistry-Paraffin: MMP-12 Antibody [NBP1-31225] - DLD1 xenograft, using MMP12 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/MMP-12-Antibody-Immunohistochemistry-Paraffin-NBP1-31225-img0008.jpg)
![Immunohistochemistry-Paraffin: MMP-12 Antibody [NBP1-31225] - Human lung adenocarcinoma. MMP12 antibody [N3C1], Internal diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/MMP-12-Antibody-Immunohistochemistry-Paraffin-NBP1-31225-img0012.jpg)
![Immunohistochemistry: MMP-12 Antibody (706F9.01) [DDX0281P-100] - Staining of human tonsil frozen section.](http://images.novusbio.com/fullsize/MMP-12-Antibody-706F9-01-Immunohistochemistry-DDX0281P-100-img0004.jpg "Immunohistochemistry: MMP-12 Antibody (706F9.01) [DDX0281P-100] - Staining of human tonsil frozen section.")
![ELISA: MMP-12 Antibody (706F9.01) [DDX0281P-100] - ELISA with 706F9.01/701E4.03 anti-HME 1000 ng/ml1= control2= HME-transfected cells3= CD34++GMCSF+TNF- +IL44= patient serum of Langerhans histiocytosis](http://images.novusbio.com/fullsize/MMP-12-Antibody-706F9-01-ELISA-DDX0281P-100-img0006.jpg)
![Immunohistochemistry: MMP-12 Antibody (703D10) [DDX0283P-100] - Staining of human lung cancer frozen section.](http://images.novusbio.com/fullsize/MMP-12-Antibody-703D10-Immunohistochemistry-DDX0283P-100-img0005.jpg "Immunohistochemistry: MMP-12 Antibody (703D10) [DDX0283P-100] - Staining of human lung cancer frozen section.")
![Flow Cytometry: MMP-12 Antibody (703D10) [DDX0283P-100] - Intracellular staining of canine cells](http://images.novusbio.com/fullsize/MMP-12-Antibody-703D10-Flow-Cytometry-DDX0283P-100-img0003.jpg)
![ELISA: MMP-12 Antibody (703D10) [DDX0283P-100] - HumanHME-MMP12 ELISA assay](http://images.novusbio.com/fullsize/MMP-12-Antibody-703D10-ELISA-DDX0283P-100-img0006.jpg)
![Immunohistochemistry-Frozen: MMP-12 Antibody (603E6.22) [DDX0280P-100] - Analysis of HRP conjugate of DDX0280P-100. Staining of human lung cancer frozen section.](http://images.novusbio.com/fullsize/MMP-12-Antibody-603E6-22-Immunohistochemistry-Frozen-DDX0280P-100-img0007.jpg "Immunohistochemistry-Frozen: MMP-12 Antibody (603E6.22) [DDX0280P-100] - Analysis of HRP conjugate of DDX0280P-100. Staining of human lung cancer frozen section.")
![Immunohistochemistry: MMP-12 Antibody (603E6.22) [DDX0280P-100] - Analysis of human lung cancer frozen section with clone 603E6 (DX0280)](http://images.novusbio.com/fullsize/MMP-12-Antibody-603E6-22-Immunohistochemistry-DDX0280P-100-img0006.jpg)
![Immunohistochemistry: MMP-12 Antibody (603E6.22) [DDX0280P-100] - Human lung tissue sections](http://images.novusbio.com/fullsize/MMP-12-Antibody-603E6-22-Immunohistochemistry-DDX0280P-100-img0008.jpg)
![ELISA: MMP-12 Antibody (701E4.03) [DDX0284P-100] - 1= control2= HME-transfected cells3= CD34++GMCSF+TNF- +IL44= patient serum of Langerhans histiocytosis](http://images.novusbio.com/fullsize/MMP-12-Antibody-701E4-03-ELISA-DDX0284P-100-img0002.jpg "ELISA: MMP-12 Antibody (701E4.03) [DDX0284P-100] - 1= control2= HME-transfected cells3= CD34++GMCSF+TNF- +IL44= patient serum of Langerhans histiocytosis")
![Immunohistochemistry: MMP-12 Antibody (RM381)[NBP3-18826]Immunohistochemical staining of formalin fixed and paraffin embedded human lung tissue section using anti-MMP-12 rabbit monoclonal antibody (Clone RM381) at a 1:2500 dilution.](http://images.novusbio.com/fullsize/nbp3-18826_rabbit-mmp-12-mab-rm381-161120231301820.png "Immunohistochemistry: MMP-12 Antibody (RM381)[NBP3-18826]Immunohistochemical staining of formalin fixed and paraffin embedded human lung tissue section using anti-MMP-12 rabbit monoclonal antibody (Clone RM381) at a 1:2500 dilution.")
![MMP-12 Antibody (RM381) [NBP3-18826] - Western Blot of HEK 293 cells lysate using NBP3-18826 at a 1:500 dilution.](http://images.novusbio.com/fullsize/nbp3-18826_rabbit-mmp-12-mab-rm381-206202415324916.png)
![Immunohistochemistry: MMP-12 Antibody (2G3) [NBP3-26138] - Image of MMP-12 Antibody (2G3) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26138_rabbit-mmp-12-mab-2g3-262202414471567.jpg "Immunohistochemistry: MMP-12 Antibody (2G3) [NBP3-26138] - Image of MMP-12 Antibody (2G3) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: MMP-12 Antibody (2G3) [NBP3-26138] - Positive Western Blot detected in: 293T whole cell lysate, MCF-7 whole cell lysate, THP-1 whole cell lysate. All lanes: MMP-12 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 55 kDa Observed band size: 54, 45 kDa](http://images.novusbio.com/fullsize/nbp3-26138_rabbit-mmp-12-mab-2g3-28220241147216.jpg)
![ELISA: MMP-12 Antibody (706F9.01) [HRP] [DDX0281HRPO] - [DDX0281HRPO] - 1= control; 2=HME-transfected cells; 3=CD34+ +GMCSF+TNFa+IL4; 4=patient serum of Langerhans hystiocytosis (samples were tested with DDX0281).](http://images.novusbio.com/fullsize/MMP-12-Antibody-706F9-01-HRP-ELISA-DDX0281HRPO-img0002.jpg "ELISA: MMP-12 Antibody (706F9.01) [HRP] [DDX0281HRPO] - [DDX0281HRPO] - 1= control; 2=HME-transfected cells; 3=CD34+ +GMCSF+TNFa+IL4; 4=patient serum of Langerhans hystiocytosis (samples were tested with DDX0281).")