GSDMDC1 Antibodies
We offer GSDMDC1 Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot. Each GSDMDC1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our GSDMDC1 Antibodies can be used in a variety of model species: Human. Use the list below to choose the GSDMDC1 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our GSDMDC1 polyclonal antibodies and browse our GSDMDC1 monoclonal antibody catalog.
Alternate Names for GSDMDC1 Antibodies
anti-GSDMDC1 antibody, anti-GSDMD antibody, anti-DF5L antibody, anti-DFNA5L antibody, anti-FLJ12150 antibody, anti-gasdermin D antibody, anti-gasdermin domain containing 1 antibody, anti-Gasdermin domain-containing protein 1 antibody, anti-gasdermin-D antibody
14 Results for "gsdmdc1" in Primary Antibodies
![Western Blot: GSDMDC1 Antibody [NBP2-33422] - miR-513c-5p negatively regulates caspase-1 expression in HUVECs. (A) The expression of miR-513c-5p after NC, miR-513c-5p mimics, INC & miR-513c-5p inhibitor transfection was evaluated by qRT-PCR. (B) The expression level of caspase-1 mRNA after NC, miR-513c-5p mimics, INC & miR-513c-5p inhibitor transfection was detected by qRT-PCR. (C,D) Expression of caspase-1 in HUVECs were detected after transfection NC, miR-513c-5p mimics, INC, miR-513c-5p inhibitor by immunofluorescence staining (magnification, ×40). Scale bar = 200 μm. (E,F) Protein expression levels of caspase-1 & GSDMD were examined by Western blot. (G) The expression of IL-1 beta & IL-18 was detected by ELISA. *p 0.05; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35463001), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-33422_rabbit-polyclonal-gsdmdc1-antibody-31020241613629.jpg)
![Western Blot: GSDMDC1 Antibody [NBP2-33422] - Pyroptosis is occurred in mouse corneas of C. albicans keratitis. (A) RT-qPCR analysis of the mRNA levels of pyroptosis-associated genes (ASC/CASP1/GSDMD/IL-1 beta /IL-18) in mouse corneas at 0 (control), 1, 3, & 7 dpi (n = 3). (B,C) Western blot detecting pyroptosis-related proteins of ASC, cleaved CASP1, cleaved IL-1 beta , cleaved IL-18, F-GSDMD & N-GSDMD in mouse corneas at 0 (control), 1, 3, & 7 dpi (n = 3). (D) Double-immunofluorescence staining of CASP1 & TUNEL in C. albicans infected-corneas compared with mock-infected controls (n = 3; Scale bar = 20 μm; magnification 400×). CASP1: caspase-1; Clv-CASP1:cleaved CASP1; Clv-IL-1 beta :cleaved IL-1 beta ; Clv-IL-18:cleaved IL-18; F-GSDMD: p53 form of GSDMD; N-GSDMD: cleaved p30 form of GSDMD. All values are presented as mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001 vs. control group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35463001), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-33422_rabbit-polyclonal-gsdmdc1-antibody-310202416114767.jpg)
![Western Blot: GSDMDC1 Antibody [NBP2-33422] - NLRP3 knockdown attenuates the pyroptosis in mouse C. albicans keratitis. (A–C) RT-qPCR analysis & western blot showing the mRNA & protein levels of pyroptosis-related molecules in C. albicans-infected corneas pretreated with Ad-GFP-shRNA & Ad-NLRP3- shRNA compared with mock controls (n = 3). (D) Immunofluorescence staining of ASC, CASP1 & GSDMD in C. albicans-infected corneas pretreated with Ad-GFP-shRNA & Ad-NLRP3- shRNA compared with mock controls (n = 3). (E) Double-immunofluorescence staining of CASP1 & TUNEL in the mice cornea of Ad-NLRP3-shRNA group compared with the Ad-GFP-shRNA group (n = 3). Scale bar = 20 μm; magnification 400×. FK: fungal keratitis. CASP1: caspase-1; Clv-CASP1:cleaved CASP1; Clv-IL-1 beta :cleaved IL-1 beta ; Clv-IL-18:cleaved IL-18; F-GSDMD: p53 form of GSDMD; N-GSDMD: cleaved p30 form of GSDMD. All values are presented as mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35463001), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-33422_rabbit-polyclonal-gsdmdc1-antibody-31020241613636.jpg)
![Western Blot: GSDMDC1 Antibody [NBP2-33422] - Knockdown of miR-513c-5p promotes pyroptosis of VECs & DVT formation by increasing caspase-1. (A) The expression level of miR-513c-5p in vascular tissue was detected by qRT-PCR in DVT mice treated with INC, miR-513c-5p inhibitor, respectively. (B) Expression of miR-513c-5p in PBMCs was detected by qRT-PCR in DVT mice treated with INC, miR-513c-5p inhibitor, respectively. (C) Confocal microscopy images of miR-513c-5p expression in vascular tissues (miR-513c-5p, red; DAPI, blue) (magnification, ×200). Scale bars = 100 μm. (D) H&E staining of serial cross sections of inferior vena cava (IVC) from DVT mice treated with INC, miR-513c-5p inhibitor at 48 h (magnification, ×40). Scale bars = 500 μm. (E) Representative images of thrombi in each treatment group were detected by vascular ultrasound at 48 h post-operation. (F) Thrombus length & weight at 48 h post-operation in the different treatment groups (n = 15 in each group). (G) Caspase-1 mRNA level was determined by qRT-PCR in vascular tissue of each treatment group. (H) Caspase-1 & GSDMD protein levels were determined by Western blot in vascular tissue of DVT mice treated with INC, miR-513c-5p inhibitor. (I) IL-1 beta & IL-18 protein levels in vascular tissue were determined by ELISA in INC, miR-513c-5p inhibitor treated DVT mice models, respectively. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35445025), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-33422_rabbit-polyclonal-gsdmdc1-antibody-310202416114772.jpg)
![Western Blot: GSDMDC1 Antibody [NBP2-33422] - Pyroptosis & IL-1 beta release is dependent on the B. pseudomallei T3SS-3.hMDMs were infected with B. pseudomallei (MOI 300) for 3h. (A & B) Cell death induction & intracellular bacterial burden were determined at 0 & 3h p.i. Shown are the median & interquartile range of at least three independent experiments with different donors performed in technical duplicates. (C) IL-1 beta secretion was determined 3h p.i. Data are presented as median & interquartile range of four independent experiments with different donors performed in duplicates. (D) Caspase-1 & gasdermin-D processing were investigated 3h p.i. Lysates were re-probed for beta -actin. For immunoblot analysis one representative experiment of at least three with different donors is shown. (E) Growth analysis of wt & delta bsaL was performed. Shown are mean values of three independent experiments performed in technical triplicates. (*p < 0.05, **p < 0.01). n.i. (not infected), wt (wild type), b.d. (below detection), hours (h), p.i. (post infection), GSDMD (gasdermin D). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33137811), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-33422_rabbit-polyclonal-gsdmdc1-antibody-310202416121947.jpg)
![Western Blot: GSDMDC1 Antibody [NBP2-33422] - Priming is not required for NLRP3 inflammasome activation in human monocytes in vitro. (A, B) Undifferentiated THP-1 cells (n=6 independent biological replicates) & (C, D) primary CD14+ monocytes (n=6 independent biological replicates (each point represents a different blood donor)) were left untreated or primed with LPS (1 µg/ml, 4 h) prior to treatment with nigericin (10 µM, 45 min) to activate the NLRP3 inflammasome. (A, C) IL-1 beta & IL-18 were measured by ELISA & cell death was measured by LDH assay & shown as percentage relative to total cell death, mean ± S.D., *P < 0.05; **P < 0.01; ***P < 0.001; ns (non significant) using one-way ANOVA comparing all groups. (B, D) Western blot analysis for mIL-18 (18 kDa), pro-IL-18 (24 kDa), mIL-1 beta (17 kDa), pro-IL-1 beta (34 kDa), mCaspase-1 (20 kDa), pro-Caspase-1 (45 kDa), GSDMD full length (FL, 53 kDa), GSDMD N-terminus (NT, 31 kDa), UBE2L3 (17.9 kDa), NLRP3 (113 kDa), as well as loading control beta -actin (42 kDa). Blots are representative of at least 3 independent biological experiments & in case of monocytes 3 different blood donors. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33101286), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-33422_rabbit-polyclonal-gsdmdc1-antibody-310202416121939.jpg)
![Western Blot: GSDMDC1 Antibody [NBP2-33422] - Heat-killed C. albicans (HKCA) activates NLRP3 inflammasome & induces pyroptosis in human corneal epithelial cells (HCECs). (A) The mRNA expression of NLRP3 in HCECs challenged with HKCA at an MOI of 1:500, 1:50, 1:5, 2:1, or 20:1 respectively for 4 hours was evaluated by RT-qPCR (n = 5). (B–D) The mRNA & protein expression of NLRP3 in HCECs exposed to HKCA (MOI = 20) for 0 (control), 2, 4, 8, 12, or 24 h (n = 3). (E) NLRP3 fluorescence intensity was evaluated using immunofluorescent staining for different times (12–36 h). (n = 3; Scale bar = 20 μm; magnification 400×). (F) Lactate dehydrogenase (LDH) of HCECs treated with HKCA (MOI = 20) for 24 h (n = 6). (G) The mRNA levels of ASC, CASP1, IL-1 beta , IL-18 & GSDMD in HCECs exposed to HKCA (MOI = 20) for different times (n = 3). (H,I) The protein expression of pyroptosis-related proteins (ASC, cleaved CASP1, N-GSDMD, cleaved IL-1 beta & cleaved IL-18) was examined by western blot (n = 3). CASP1: caspase-1; Clv-CASP1: cleaved CASP1; Clv-IL-1 beta : cleaved IL-1 beta ; Clv-IL-18: cleaved IL-18; N-GSDMD: cleaved p30 form of GSDMD. All values are presented as mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001 vs. control group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35463001), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-33422_rabbit-polyclonal-gsdmdc1-antibody-31020241612228.jpg)
![Western Blot: GSDMDC1 Antibody [NBP2-80427] - Analysis of Gasdermin D (N terminal) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SiHa cell lysateLane 2: human kidney tissue lysate](http://images.novusbio.com/fullsize/GSDMDC1-Antibody-Western-Blot-NBP2-80427-img0009.jpg "Western Blot: GSDMDC1 Antibody [NBP2-80427] - Analysis of Gasdermin D (N terminal) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SiHa cell lysateLane 2: human kidney tissue lysate")
![Immunocytochemistry/Immunofluorescence: GSDMDC1 Antibody [NBP2-80427] - Staining of Gasdermin D in SiHa cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1901-37, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).](http://images.novusbio.com/fullsize/GSDMDC1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-80427-img0002.jpg)
![Immunohistochemistry-Paraffin: GSDMDC1 Antibody [NBP2-80427] - Analysis in Mouse colon tissue using GSDMDC1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with](http://images.novusbio.com/fullsize/GSDMDC1-Antibody-Immunohistochemistry-Paraffin-NBP2-80427-img0007.jpg)
![Flow Cytometry: GSDMDC1 Antibody [NBP2-80427] - Analysis of GSDMDC1 was done on SiHa cells. The cells were fixed, permeabilized and stained with the primary antibody (1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/GSDMDC1-Antibody-Flow-Cytometry-NBP2-80427-img0001.jpg)
![Immunohistochemistry-Paraffin: GSDMDC1 Antibody [NBP2-80427] - Analysis in rat stomach tissue using GSDMDC1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with](http://images.novusbio.com/fullsize/GSDMDC1-Antibody-Immunohistochemistry-Paraffin-NBP2-80427-img0003.jpg)
![Immunohistochemistry-Paraffin: GSDMDC1 Antibody [NBP2-80427] - Analysis in human tonsil tissue using GSDMDC1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed wit](http://images.novusbio.com/fullsize/GSDMDC1-Antibody-Immunohistochemistry-Paraffin-NBP2-80427-img0004.jpg)
![Immunohistochemistry-Paraffin: GSDMDC1 Antibody [NBP2-80427] - Analysis in human prostate carcinoma tissue using GSDMDC1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature](http://images.novusbio.com/fullsize/GSDMDC1-Antibody-Immunohistochemistry-Paraffin-NBP2-80427-img0005.jpg)
![Immunohistochemistry-Paraffin: GSDMDC1 Antibody [NBP2-80427] - Analysis in human esophagus tissue using GSDMDC1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed](http://images.novusbio.com/fullsize/GSDMDC1-Antibody-Immunohistochemistry-Paraffin-NBP2-80427-img0006.jpg)
![Western Blot: GSDMDC1 Antibody [NBP2-93428] - Western blot analysis of extracts of various cell lines, using GSDMDC1 antibody (NBP2-93428) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/GSDMDC1 Antibody-Western Blot-NBP2-93428-img0004.jpg "Western Blot: GSDMDC1 Antibody [NBP2-93428] - Western blot analysis of extracts of various cell lines, using GSDMDC1 antibody (NBP2-93428) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunocytochemistry/Immunofluorescence: GSDMDC1 Antibody [NBP2-93428] - Immunofluorescence analysis of U2OS cells using GSDMDC1 Rabbit pAb (NBP2-93428) at dilution of 1:250 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/GSDMDC1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-93428-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: GSDMDC1 Antibody [NBP2-93428] - Immunofluorescence analysis of NIH/3T3 cells using GSDMDC1 Rabbit pAb (NBP2-93428) at dilution of 1:250 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/GSDMDC1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-93428-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: GSDMDC1 Antibody [NBP2-93428] - Immunofluorescence analysis of PC-12 cells using GSDMDC1 Rabbit pAb (NBP2-93428) at dilution of 1:250 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/GSDMDC1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-93428-img0002.jpg)
![Western Blot: GSDMDC1 Antibody (HL1430) - Azide and BSA Free [NBP3-25478] - Untreated (-) and treated (+) THP-1 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Gasdermin D antibody [HL1430] (NBP3-25478) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25478_rabbit-gsdmdc1-mab-hl1430-azide-and-bsa-free-2120249351326.jpg "Western Blot: GSDMDC1 Antibody (HL1430) - Azide and BSA Free [NBP3-25478] - Untreated (-) and treated (+) THP-1 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Gasdermin D antibody [HL1430] (NBP3-25478) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: GSDMDC1 Antibody (HL1430) - Azide and BSA Free [NBP3-25478] - Gasdermin D antibody [HL1430] detects Gasdermin D protein at cell membrane and secreted by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Gasdermin D stained by Gasdermin D antibody [HL1430] (NBP3-25478) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25478_rabbit-gsdmdc1-mab-hl1430-azide-and-bsa-free-2120249393571.jpg)
![Western Blot: GSDMDC1 Antibody (8A8A5) [NBP3-16070] - Western blot analysis of extracts of THP-1 cells, using GSDMDC1 antibody (NBP3-16070) at 1:1000 dilution.THP-1 cells were treated by LPS (1 ug/ml) at 37C for 8 hours.THP-1 cells were treated by PMA/TPA (200 nM) at 37C for 15 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/GSDMDC1-Antibody-8A8A5-Western-Blot-NBP3-16070-img0004.jpg "Western Blot: GSDMDC1 Antibody (8A8A5) [NBP3-16070] - Western blot analysis of extracts of THP-1 cells, using GSDMDC1 antibody (NBP3-16070) at 1:1000 dilution.THP-1 cells were treated by LPS (1 ug/ml) at 37C for 8 hours.THP-1 cells were treated by PMA/TPA (200 nM) at 37C for 15 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Western Blot: GSDMDC1 Antibody (8A8A5) [NBP3-16070] - Western blot analysis of extracts of A-431 cells, using GSDMDC1 antibody (NBP3-16070) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/GSDMDC1-Antibody-8A8A5-Western-Blot-NBP3-16070-img0005.jpg)
![Western Blot: GSDMDC1 Antibody [H00079792-D01P] - Analysis of GSDMD expression in transfected 293T cell line by GSDMD polyclonal antibody.Lane 1: GSDMDC1 transfected lysate(52.80 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/GSDMDC1-Antibody-Western-Blot-H00079792-D01P-img0002.jpg "Western Blot: GSDMDC1 Antibody [H00079792-D01P] - Analysis of GSDMD expression in transfected 293T cell line by GSDMD polyclonal antibody.Lane 1: GSDMDC1 transfected lysate(52.80 KDa).Lane 2: Non-transfected lysate.")
![Western Blot: GSDMDC1 Antibody [NBP2-14973] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GSDMDC1-Antibody-Western-Blot-NBP2-14973-img0002.jpg "Western Blot: GSDMDC1 Antibody [NBP2-14973] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: GSDMDC1 Antibody [NBP2-14973] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Gasdermin D antibody [N1N3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp2-14973_rabbit-polyclonal-gsdmdc1-antibody-1610202419405758.jpg)
![Western Blot: GSDMDC1 Antibody (PD00-18) - N-terminal [NBP3-32369] - Western blot analysis of GSDMDC1 on different lysates with Rabbit anti-GSDMDC1 antibody (NBP3-32369) at 1/1,000 dilution. Lane 1: SiHa cell lysate Lane 2: Jurkat cell lysate Lane 3: THP-1 cell lysate Lane 4: PC-3M cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: PC-12 cell lysate Lane 7: L6 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53/30 kDa Observed band size: 53/30 kDa Exposure time: 43 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32369) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32369_rabbit-gsdmdc1-mab-pd00-18-n-terminal-278202416331282.jpg "Western Blot: GSDMDC1 Antibody (PD00-18) - N-terminal [NBP3-32369] - Western blot analysis of GSDMDC1 on different lysates with Rabbit anti-GSDMDC1 antibody (NBP3-32369) at 1/1,000 dilution. Lane 1: SiHa cell lysate Lane 2: Jurkat cell lysate Lane 3: THP-1 cell lysate Lane 4: PC-3M cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: PC-12 cell lysate Lane 7: L6 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53/30 kDa Observed band size: 53/30 kDa Exposure time: 43 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32369) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: GSDMDC1 Antibody (HL1334) - Azide and BSA Free [NBP3-25477] - Untreated (-) and treated (+) THP-1 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Gasdermin D antibody [HL1334] (NBP3-25477) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25477_rabbit-gsdmdc1-mab-hl1334-azide-and-bsa-free-2120249355580.jpg "Western Blot: GSDMDC1 Antibody (HL1334) - Azide and BSA Free [NBP3-25477] - Untreated (-) and treated (+) THP-1 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Gasdermin D antibody [HL1334] (NBP3-25477) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: GSDMDC1 Antibody [H00079792-B01P] - Analysis of GSDMD expression in transfected 293T cell line by GSDMD polyclonal antibody. Lane1:GSDMDC1 transfected lysate(53.24 KDa). Lane2:Non-transfected lysate.](http://images.novusbio.com/fullsize/GSDMDC1-Antibody-Western-Blot-H00079792-B01P-img0002.jpg "Western Blot: GSDMDC1 Antibody [H00079792-B01P] - Analysis of GSDMD expression in transfected 293T cell line by GSDMD polyclonal antibody. Lane1:GSDMDC1 transfected lysate(53.24 KDa). Lane2:Non-transfected lysate.")