FAK Antibodies
We offer FAK Antibodies for use in common research applications: ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Proximity Ligation Assay, Sandwich ELISA, Western Blot. Each FAK Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our FAK Antibodies can be used in a variety of model species: Amphibian, Bat, Bovine, Canine, Chicken, Equine, Hamster, Human, Monkey, Mouse, Primate, Rabbit, Rat. Use the list below to choose the FAK Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our FAK polyclonal antibodies and browse our FAK monoclonal antibody catalog.
Alternate Names for FAK Antibodies
anti-FAK antibody, anti-PTK2 antibody, anti-EC 2.7.10 antibody, anti-FADK 1 antibody, anti-FADKFAK-related non-kinase polypeptide antibody, anti-FAK1FRNK antibody, anti-FAKEC 2.7.10.2 antibody, anti-focal adhesion kinase 1 antibody, anti-pp125FAK antibody, anti-Protein-tyrosine kinase 2 antibody, anti-PTK2 protein tyrosine kinase 2 antibody
19 Results for "fak" in Primary Antibodies
![Western Blot: FAK Antibody (SR46-04) [NBP2-67327] - Western blot analysis of FAK on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbi](http://images.novusbio.com/fullsize/FAK-Antibody-SR46-04-Western-Blot-NBP2-67327-img0012.jpg "Western Blot: FAK Antibody (SR46-04) [NBP2-67327] - Western blot analysis of FAK on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbi")
![Immunocytochemistry/Immunofluorescence: FAK Antibody (SR46-04) [NBP2-67327] - Staining FAK in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/FAK-Antibody-SR46-04-Immunocytochemistry-Immunofluorescence-NBP2-67327-img0007.jpg)
![Immunohistochemistry-Paraffin: FAK Antibody (SR46-04) [NBP2-67327] - Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-FAK antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 m](http://images.novusbio.com/fullsize/FAK-Antibody-SR46-04-Immunohistochemistry-Paraffin-NBP2-67327-img0011.jpg)
![Flow Cytometry: FAK Antibody (SR46-04) [NBP2-67327] - Analysis of Hela cells with FAK antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/FAK-Antibody-SR46-04-Flow-Cytometry-NBP2-67327-img0001.jpg)
![Western Blot: FAK Antibody (SR46-04) [NBP2-67327] - Analysis of FAK on different lysates using anti-FAK antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: Mouse spleen](http://images.novusbio.com/fullsize/FAK-Antibody-SR46-04-Western-Blot-NBP2-67327-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: FAK Antibody (SR46-04) [NBP2-67327] - Staining FAK in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/FAK-Antibody-SR46-04-Immunocytochemistry-Immunofluorescence-NBP2-67327-img0002.jpg)
![Immunohistochemistry-Paraffin: FAK Antibody (SR46-04) [NBP2-67327] - Analysis of paraffin-embedded human spleen tissue using anti-FAK antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/FAK-Antibody-SR46-04-Immunohistochemistry-Paraffin-NBP2-67327-img0003.jpg)
![Immunohistochemistry-Paraffin: FAK Antibody (SR46-04) [NBP2-67327] - Analysis of paraffin-embedded mouse brain tissue using anti-FAK antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/FAK-Antibody-SR46-04-Immunohistochemistry-Paraffin-NBP2-67327-img0004.jpg)
![Immunohistochemistry-Paraffin: FAK Antibody (SR46-04) [NBP2-67327] - Analysis of paraffin-embedded mouse spleen tissue using anti-FAK antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/FAK-Antibody-SR46-04-Immunohistochemistry-Paraffin-NBP2-67327-img0005.jpg)
![Immunohistochemistry-Paraffin: FAK Antibody (SR46-04) [NBP2-67327] - Analysis of paraffin-embedded huamn liver cancer tissue using anti-FAK antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/FAK-Antibody-SR46-04-Immunohistochemistry-Paraffin-NBP2-67327-img0008.jpg)
![Immunohistochemistry-Paraffin: FAK Antibody (SR46-04) [NBP2-67327] - Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-FAK antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 m](http://images.novusbio.com/fullsize/FAK-Antibody-SR46-04-Immunohistochemistry-Paraffin-NBP2-67327-img0009.jpg)
![Immunohistochemistry-Paraffin: FAK Antibody (SR46-04) [NBP2-67327] - Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-FAK antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 m](http://images.novusbio.com/fullsize/FAK-Antibody-SR46-04-Immunohistochemistry-Paraffin-NBP2-67327-img0010.jpg)
![Western Blot: FAK [p Tyr397] Antibody [NBP3-12897] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr397) antibody (NBP3-12897) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/FAK-[p-Tyr397]-Antibody-Western-Blot-NBP3-12897-img0007.jpg "<b>Genetic Strategies Validation. </b>Western Blot: FAK [p Tyr397] Antibody [NBP3-12897] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr397) antibody (NBP3-12897) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: FAK [p Tyr397] Antibody [NBP3-12897] - Untreated (-) and treated (+) HepG2 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr397) antibody (NBP3-12897) diluted at 1:500.](http://images.novusbio.com/fullsize/FAK-[p-Tyr397]-Antibody-Western-Blot-NBP3-12897-img0001.jpg)
![Western Blot: FAK [p Tyr397] Antibody [NBP3-12897] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr397) antibody (NBP3-12897) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/FAK-[p-Tyr397]-Antibody-Western-Blot-NBP3-12897-img0002.jpg)
![Western Blot: FAK [p Tyr397] Antibody [NBP3-12897] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr397) antibody (NBP3-12897) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/FAK-[p-Tyr397]-Antibody-Western-Blot-NBP3-12897-img0003.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: FAK [p Tyr397] Antibody [NBP3-12897] - Untreated (-) and treated (+) HepG2 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr397) antibody (NBP3-12897) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/FAK-[p-Tyr397]-Antibody-Western-Blot-NBP3-12897-img0004.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: FAK [p Tyr397] Antibody [NBP3-12897] - Untreated (-) and treated (+) HUVEC whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr397) antibody (NBP3-12897) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/FAK-[p-Tyr397]-Antibody-Western-Blot-NBP3-12897-img0006.jpg)
![Immunocytochemistry/ Immunofluorescence: FAK [p Tyr397] Antibody [NBP3-12897] - FAK antibody detects FAK protein at cell junction, nucleus and cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: FAK stained by FAK antibody (NBP3-12897) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp3-12897_rabbit-fak-p-tyr-397-pab-910202419593568.jpg)
![Western Blot: FAK [p Tyr397] Antibody [NBP3-12897] - Untreated (-) and treated (+) HepG2 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with FAK antibody (NBP3-12897) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-12897_rabbit-fak-p-tyr-397-pab-91020242014611.jpg)
![Western Blot: FAK Antibody (OTI4D11) [NBP2-45923] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FAK .](http://images.novusbio.com/fullsize/FAK-Antibody-OTI4D11-Western-Blot-NBP2-45923-img0008.jpg "Western Blot: FAK Antibody (OTI4D11) [NBP2-45923] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FAK .")
![Immunocytochemistry/Immunofluorescence: FAK Antibody (OTI4D11) [NBP2-45923] - FAK Antibody (4D11) [NBP2-45923] - Analysis of HeLa cells.](http://images.novusbio.com/fullsize/FAK-Antibody-OTI4D11-Immunocytochemistry-Immunofluorescence-NBP2-45923-img0002.jpg)
![Immunohistochemistry: FAK Antibody (OTI4D11) [NBP2-45923] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/FAK-Antibody-OTI4D11-Immunohistochemistry-NBP2-45923-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: FAK Antibody (OTI4D11) [NBP2-45923] - FAK Antibody (4D11) [NBP2-45923] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY FAK.](http://images.novusbio.com/fullsize/FAK-Antibody-OTI4D11-Immunocytochemistry-Immunofluorescence-NBP2-45923-img0001.jpg)
![Immunohistochemistry: FAK Antibody (OTI4D11) [NBP2-45923] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/FAK-Antibody-OTI4D11-Immunohistochemistry-NBP2-45923-img0003.jpg)
![Immunohistochemistry: FAK Antibody (OTI4D11) [NBP2-45923] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/FAK-Antibody-OTI4D11-Immunohistochemistry-NBP2-45923-img0004.jpg)
![Immunohistochemistry: FAK Antibody (OTI4D11) [NBP2-45923] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/FAK-Antibody-OTI4D11-Immunohistochemistry-NBP2-45923-img0005.jpg)
![Immunohistochemistry: FAK Antibody (OTI4D11) [NBP2-45923] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/FAK-Antibody-OTI4D11-Immunohistochemistry-NBP2-45923-img0006.jpg)
![Western Blot: FAK [p Tyr576] Antibody [NBP3-13071] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr576) antibody (NBP3-13071) diluted at 1:500.](http://images.novusbio.com/fullsize/FAK-[p-Tyr576]-Antibody-Western-Blot-NBP3-13071-img0008.jpg "<b>Genetic Strategies Validation. </b>Western Blot: FAK [p Tyr576] Antibody [NBP3-13071] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr576) antibody (NBP3-13071) diluted at 1:500.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: FAK [p Tyr576] Antibody [NBP3-13071] - FAK (phospho Tyr576) antibody detects FAK (phospho Tyr576) protein at cell membrane and nucleus by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: FAK (phospho Tyr576) protein stained by FAK (phospho Tyr576) antibody (NBP3-13071) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/FAK-[p-Tyr576]-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13071-img0002.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: FAK [p Tyr576] Antibody [NBP3-13071] - FAK (phospho Tyr576) antibody detects FAK (phospho Tyr576) protein by western blot analysis. Left: Untreated (-) and EGF-treated (+, 50 ng/ml for 30 min) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE. Right: Untreated (-) and EGF-treated (+, 200 ng/ml for 30 min) HepG2 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE. The membranes were blotted with FAK (phospho Tyr576) antibody (NBP3-13071) diluted at 1:3000.](http://images.novusbio.com/fullsize/FAK-[p-Tyr576]-Antibody-Western-Blot-NBP3-13071-img0001.jpg)
![Western Blot: FAK [p Tyr576] Antibody [NBP3-13071] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr576) antibody (NBP3-13071) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/FAK-[p-Tyr576]-Antibody-Western-Blot-NBP3-13071-img0003.jpg)
![Western Blot: FAK [p Tyr576] Antibody [NBP3-13071] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr576) antibody (NBP3-13071) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/FAK-[p-Tyr576]-Antibody-Western-Blot-NBP3-13071-img0004.jpg)
![Western Blot: FAK [p Tyr576] Antibody [NBP3-13071] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr576) antibody (NBP3-13071) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/FAK-[p-Tyr576]-Antibody-Western-Blot-NBP3-13071-img0005.jpg)
![Western Blot: FAK [p Tyr576] Antibody [NBP3-13071] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr576) antibody (NBP3-13071) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/FAK-[p-Tyr576]-Antibody-Western-Blot-NBP3-13071-img0006.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: FAK [p Tyr576] Antibody [NBP3-13071] - Untreated (-) and treated (+) HepG2 whole cell extract (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr576) antibody (NBP3-13071) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/FAK-[p-Tyr576]-Antibody-Western-Blot-NBP3-13071-img0007.jpg)
![Western Blot: FAK [p Tyr576] Antibody [NBP3-13071] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with FAK antibody (NBP3-13071) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13071_rabbit-fak-p-tyr-576-pab-91020242041236.jpg)
![Western Blot: FAK [p Tyr576] Antibody [NBP3-13071] - Untreated (-) and treated (+) HepG2 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with FAK antibody (NBP3-13071) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13071_rabbit-fak-p-tyr-576-pab-9102024205680.jpg)
![Immunohistochemistry-Paraffin: FAK [p Tyr576] Antibody [NBP3-13071] - FAK antibody detects FAK protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded rat lung.FAK stained by FAK antibody (NBP3-13071) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13071_rabbit-fak-p-tyr-576-pab-910202420115066.jpg)
![Immunocytochemistry/Immunofluorescence: FAK Antibody [NBP1-84750] - Membrane surface receptor expression and metabolomic analysis of cells grown on porcine breast fatty tissue ECM hydrogel. Integrin beta1 (a) or beta4 (b) receptor (green) and FAK (red) expression in MM231 cells cultured on the ECM hydrogel-coated coverslips were assessed by IF. Phalloidin staining (white) of F-actin was used to contour the cells. Scale bars, 10 um. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/1422-0067/19/10/2912), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/FAK-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84750-img0012.jpg "Immunocytochemistry/Immunofluorescence: FAK Antibody [NBP1-84750] - Membrane surface receptor expression and metabolomic analysis of cells grown on porcine breast fatty tissue ECM hydrogel. Integrin beta1 (a) or beta4 (b) receptor (green) and FAK (red) expression in MM231 cells cultured on the ECM hydrogel-coated coverslips were assessed by IF. Phalloidin staining (white) of F-actin was used to contour the cells. Scale bars, 10 um. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/1422-0067/19/10/2912), licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: FAK Antibody [NBP1-84750] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/FAK-Antibody-Immunohistochemistry-Paraffin-NBP1-84750-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: FAK Antibody [NBP1-84750] - Staining of human cell line U-2 OS shows localization to cytosol & focal adhesion sites. Antibody staining shown in green.](http://images.novusbio.com/fullsize/FAK-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84750-img0005.jpg)
![Immunohistochemistry-Paraffin: FAK Antibody [NBP1-84750] - Staining of human fallopian tube shows moderate cytoplasmic and membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/FAK-Antibody-Immunohistochemistry-Paraffin-NBP1-84750-img0007.jpg)
![Immunohistochemistry-Paraffin: FAK Antibody [NBP1-84750] - Staining of human testis shows moderate to cytoplasmic positivity in spermatogonia and Leydig cells.](http://images.novusbio.com/fullsize/FAK-Antibody-Immunohistochemistry-Paraffin-NBP1-84750-img0009.jpg)
![Immunohistochemistry-Paraffin: FAK Antibody [NBP1-84750] - Staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.](http://images.novusbio.com/fullsize/FAK-Antibody-Immunohistochemistry-Paraffin-NBP1-84750-img0010.jpg)
![Immunocytochemistry/ Immunofluorescence: FAK Antibody [NBP1-84750] - Membrane surface receptor expression & metabolomic analysis of cells grown on porcine breast fatty tissue ECM hydrogel. Integrin beta 1 (a) or beta 4 (b) receptor (green) & FAK (red) expression in MM231 cells cultured on the ECM hydrogel-coated coverslips were assessed by IF. Phalloidin staining (white) of F-actin was used to contour the cells. Scale bars, 10 μm. (c) MS imaging of cross sections of the medium-conditioned blank (upper panels) & MM231 cell culture-laden (bottom panels) hydrogel scaffolds. (d) Examples of ROC (upper) & box-and-whiskers (bottom) plots from the MS imaging analysis, showing data for a compound with m/z 663.024, where differences between the hydrogel samples cultured with or without MM231 cells are clearly evident. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30257480), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-84750_rabbit-polyclonal-fak-antibody-310202415284542.jpg)
![Western Blot: FAK [p Tyr407] Antibody [NBP3-13085] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr407) antibody (NBP3-13085) diluted at 1:500.](http://images.novusbio.com/fullsize/FAK-[p-Tyr407]-Antibody-Western-Blot-NBP3-13085-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: FAK [p Tyr407] Antibody [NBP3-13085] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr407) antibody (NBP3-13085) diluted at 1:500.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: FAK [p Tyr407] Antibody [NBP3-13085] - FAK (phospho Tyr407) antibody detects FAK (phospho Tyr407) protein at focal adhesion and nucleus by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: FAK (phospho Tyr407) stained by FAK (phospho Tyr407) antibody (NBP3-13085) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:100. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/FAK-[p-Tyr407]-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13085-img0004.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: FAK [p Tyr407] Antibody [NBP3-13085] - Untreated (-) and treated (+) HepG2 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr407) antibody (NBP3-13085) diluted at 1:1000.](http://images.novusbio.com/fullsize/FAK-[p-Tyr407]-Antibody-Western-Blot-NBP3-13085-img0001.jpg)
![Western Blot: FAK [p Tyr407] Antibody [NBP3-13085] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr407) antibody (NBP3-13085) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/FAK-[p-Tyr407]-Antibody-Western-Blot-NBP3-13085-img0002.jpg)
![Western Blot: FAK [p Tyr407] Antibody [NBP3-13085] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr407) antibody (NBP3-13085) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/FAK-[p-Tyr407]-Antibody-Western-Blot-NBP3-13085-img0003.jpg)
![Western Blot: FAK [p Tyr576] Antibody (HL127) [NBP3-13675] - Untreated (-) and treated (+) PC-12 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr576) antibody [HL127] (NBP3-13675) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/FAK-p-Tyr576-Antibody-HL127-Western-Blot-NBP3-13675-img0003.jpg "<b>Biological Strategies Validation. </b>Western Blot: FAK [p Tyr576] Antibody (HL127) [NBP3-13675] - Untreated (-) and treated (+) PC-12 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr576) antibody [HL127] (NBP3-13675) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Western Blot: FAK [p Tyr576] Antibody (HL127) [NBP3-13675] - HepG2 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr576) antibody [HL127] (NBP3-13675) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/FAK-p-Tyr576-Antibody-HL127-Western-Blot-NBP3-13675-img0001.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: FAK [p Tyr576] Antibody (HL127) [NBP3-13675] - Untreated (-) and treated (+) NIH-3T3 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr576) antibody [HL127] (NBP3-13675) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/FAK-p-Tyr576-Antibody-HL127-Western-Blot-NBP3-13675-img0002.jpg)
![Western Blot: FAK [p Tyr576] Antibody (HL127) [NBP3-13675] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with FAK antibody [HL127] (NBP3-13675) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13675_rabbit-fak-p-tyr-576-mab-hl127-910202420131253.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: FAK [p Tyr576] Antibody (HL127) [NBP3-13675] - Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr576) antibody [HL127] diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-13675_rabbit-fak-p-tyr-576-mab-hl127-1610202419405780.jpg)