Cofilin Antibodies
We offer Cofilin Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot. Each Cofilin Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our Cofilin Antibodies can be used in a variety of model species: Canine, Chicken, Human, Mouse, Porcine, Rat. Use the list below to choose the Cofilin Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our Cofilin polyclonal antibodies and browse our Cofilin monoclonal antibody catalog.
Alternate Names for Cofilin Antibodies
anti-Cofilin antibody, anti-CFL1 antibody, anti-18 kDa phosphoprotein antibody, anti-CFL antibody, anti-cofilin 1 (non-muscle) antibody, anti-Cofilin, non-muscle isoform antibody, anti-cofilin-1 antibody, anti-p18 antibody
19 Results for "cofilin" in Primary Antibodies
![Western Blot: Cofilin Antibody (1A1) [H00001072-M04] - Analysis of CFL1 expression in NIH/3T3 (Cat # L018V1).](http://images.novusbio.com/fullsize/Cofilin-Antibody-1A1-Western-Blot-H00001072-M04-img0013.jpg "Western Blot: Cofilin Antibody (1A1) [H00001072-M04] - Analysis of CFL1 expression in NIH/3T3 (Cat # L018V1).")
![Immunocytochemistry/Immunofluorescence: Cofilin Antibody (1A1) [H00001072-M04] - Analysis of monoclonal antibody to CFL1 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/Cofilin-Antibody-1A1-Immunocytochemistry-Immunofluorescence-H00001072-M04-img0009.jpg)
![Immunohistochemistry-Paraffin: Cofilin Antibody (1A1) [H00001072-M04] - Analysis of monoclonal antibody to CFL1 on formalin-fixed paraffin-embedded human breast cancer. Antibody concentration 1.5 ug/ml](http://images.novusbio.com/fullsize/Cofilin-Antibody-1A1-Immunohistochemistry-Paraffin-H00001072-M04-img0010.jpg)
![Western Blot: Cofilin Antibody (1A1) [H00001072-M04] - Western Blot analysis of CFL1 expression in PC-12(Cat # L012V1 ).](http://images.novusbio.com/fullsize/Cofilin-Antibody-1A1-Western-Blot-H00001072-M04-img0002.jpg)
![Western Blot: Cofilin Antibody (1A1) [H00001072-M04] - Analysis of CFL1 expression in HeLa (Cat # L013V1).](http://images.novusbio.com/fullsize/Cofilin-Antibody-1A1-Western-Blot-H00001072-M04-img0011.jpg)
![Western Blot: Cofilin Antibody (1A1) [H00001072-M04] - Analysis of CFL1 expression in Raw 264.7 (Cat # L024V1).](http://images.novusbio.com/fullsize/Cofilin-Antibody-1A1-Western-Blot-H00001072-M04-img0012.jpg)
![ELISA: Cofilin Antibody (1A1) [H00001072-M04] - Detection limit for recombinant GST tagged CFL1 is approximately 10ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Cofilin-Antibody-1A1-ELISA-H00001072-M04-img0008.jpg)
![Western Blot: Cofilin Antibody (567) [NBP2-42828] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin 1 antibody [567] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Cofilin-Antibody-567-Western-Blot-NBP2-42828-img0011.jpg "Western Blot: Cofilin Antibody (567) [NBP2-42828] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin 1 antibody [567] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: Cofilin Antibody (567) [NBP2-42828] - HeLa cells were fixed in 4% paraformaldehyde at RT for 10 min. Green: Cofilin 1 protein stained by Cofilin 1 antibody diluted at 1:300. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/Cofilin-Antibody-567-Immunocytochemistry-Immunofluorescence-NBP2-42828-img0005.jpg)
![Immunohistochemistry-Paraffin: Cofilin Antibody (567) [NBP2-42828] - HBL435 xenograft. Cofilin 1 antibody [567] dilution: 1:200. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Cofilin-Antibody-567-Immunohistochemistry-Paraffin-NBP2-42828-img0002.jpg)
![Western Blot: Cofilin Antibody (567) [NBP2-42828] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extract E. 30 ug A375 whole cell lysate/extract 12 % SDS-PAGE Cofilin 1 antibody [567] dilution: 1:1000](http://images.novusbio.com/fullsize/Cofilin-Antibody-567-Western-Blot-NBP2-42828-img0003.jpg)
![Western Blot: Cofilin Antibody (567) [NBP2-42828] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin 1 antibody [567] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Cofilin-Antibody-567-Western-Blot-NBP2-42828-img0009.jpg)
![<b>Genetic Strategies Validation. </b>Knockout Validated: Cofilin Antibody (567) [NBP2-42828] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin 1 antibody [GT567]. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Cofilin-Antibody-567-Western-Blot-NBP2-42828-img0007.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: Cofilin Antibody (567) [NBP2-42828] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Cofilin 1 antibody [GT567] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp2-42828_mouse-monoclonal-cofilin-antibody-567-1610202419405739.jpg)
![Western Blot: Cofilin Antibody (GT217) [NBP3-13520] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin antibody [GT217] (NBP3-13520) diluted at 1:1000.](http://images.novusbio.com/fullsize/Cofilin-Antibody-GT217-Western-Blot-NBP3-13520-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Cofilin Antibody (GT217) [NBP3-13520] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin antibody [GT217] (NBP3-13520) diluted at 1:1000.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: Cofilin Antibody (GT217) [NBP3-13520] - Cofilin antibody [GT217] detects Cofilin protein at cytoplasm in mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. Cofilin antibody [GT217] (NBP3-13520) diluted at 1:250. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Cofilin-Antibody-GT217-Immunohistochemistry-Paraffin-NBP3-13520-img0003.jpg)
![Western Blot: Cofilin Antibody (GT217) [NBP3-13520] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin antibody [GT217] (NBP3-13520) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Cofilin-Antibody-GT217-Western-Blot-NBP3-13520-img0004.jpg)
![Western Blot: Cofilin Antibody (GT217) [NBP3-13520] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin antibody [GT217] (NBP3-13520) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Cofilin-Antibody-GT217-Western-Blot-NBP3-13520-img0005.jpg)
![Immunoprecipitation: Cofilin Antibody (GT217) [NBP3-13520] - Immunoprecipitation of Cofilin protein from 293T whole cell extracts using 5 ug of Cofilin antibody [GT217] (NBP3-13520). Western blot analysis was performed using Cofilin antibody [GT217] (NBP3-13520) diluted at 1:500. EasyBlot anti-Mouse IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/Cofilin-Antibody-GT217-Immunoprecipitation-NBP3-13520-img0002.jpg)
![Western Blot: Cofilin Antibody (GT217) [NBP3-13520] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Cofilin antibody [GT217] (NBP3-13520) diluted at 1:20000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13520_mouse-cofilin-mab-gt217-910202420134075.jpg)
![Immunocytochemistry/ Immunofluorescence: Cofilin Antibody (GT217) [NBP3-13520] - Cofilin antibody [GT217] detects Cofilin protein at cell membrane by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Cofilin stained by Cofilin antibody [GT217] (NBP3-13520) diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-13520_mouse-cofilin-mab-gt217-91020242075996.jpg)
![Western Blot: Cofilin Antibody (PSH01-44) [NBP3-32207] - Western blot analysis of Cofilin on different lysates with Rabbit anti-Cofilin antibody (NBP3-32207) at 1/1,000 dilution. Lane 1: Hela cell lysate (30 ug/Lane) Lane 2: HEK-293 cell lysate (30 ug/Lane) Lane 3: MCF7 cell lysate (30 ug/Lane) Lane 4: MDA-MB-468 cell lysate (30 ug/Lane) Lane 5: SH-SY5Y cell lysate (30 ug/Lane) Lane 6: HUVEC cell lysate (30 ug/Lane) Lane 7: Jurkat cell lysate (30 ug/Lane) Lane 8: COS-1 cell lysate (30 ug/Lane) Lane 9: VERO cell lysate (30 ug/Lane) Lane 10: NIH/3T3 cell lysate (30 ug/Lane) Lane 11: PC-12 cell lysate (30 ug/Lane) Lane 12: Neuro-2a cell lysate (30 ug/Lane) Lane 13: K-562 cell lysate (30 ug/Lane) Lane 14: A431 cell lysate (30 ug/Lane) Predicted band size: 19 kDa Observed band size: 19 kDa Exposure time: 2 minutes 37 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32207) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32207_rabbit-cofilin-mab-psh01-44-278202416285276.jpg "Western Blot: Cofilin Antibody (PSH01-44) [NBP3-32207] - Western blot analysis of Cofilin on different lysates with Rabbit anti-Cofilin antibody (NBP3-32207) at 1/1,000 dilution. Lane 1: Hela cell lysate (30 ug/Lane) Lane 2: HEK-293 cell lysate (30 ug/Lane) Lane 3: MCF7 cell lysate (30 ug/Lane) Lane 4: MDA-MB-468 cell lysate (30 ug/Lane) Lane 5: SH-SY5Y cell lysate (30 ug/Lane) Lane 6: HUVEC cell lysate (30 ug/Lane) Lane 7: Jurkat cell lysate (30 ug/Lane) Lane 8: COS-1 cell lysate (30 ug/Lane) Lane 9: VERO cell lysate (30 ug/Lane) Lane 10: NIH/3T3 cell lysate (30 ug/Lane) Lane 11: PC-12 cell lysate (30 ug/Lane) Lane 12: Neuro-2a cell lysate (30 ug/Lane) Lane 13: K-562 cell lysate (30 ug/Lane) Lane 14: A431 cell lysate (30 ug/Lane) Predicted band size: 19 kDa Observed band size: 19 kDa Exposure time: 2 minutes 37 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32207) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Cofilin Antibody (PSH01-44) [NBP3-32207] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-Cofilin antibody (NBP3-32207) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32207) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32207_rabbit-cofilin-mab-psh01-44-2982024142099.jpg)
![Immunocytochemistry/ Immunofluorescence: Cofilin Antibody (PSH01-44) [NBP3-32207] - Immunocytochemistry analysis of HeLa cells labeling Cofilin with Rabbit anti-Cofilin antibody (NBP3-32207) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Cofilin antibody (NBP3-32207) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32207_rabbit-cofilin-mab-psh01-44-2920241048483.jpg)
![Flow Cytometry: Cofilin Antibody (PSH01-44) [NBP3-32207] - Flow cytometric analysis of HeLa cells labeling Cofilin. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32207, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32207_rabbit-cofilin-mab-psh01-44-29202416372168.jpg)
![Western Blot: Cofilin Antibody [NB100-1900] - Whole extract of A-431 Human epithelial carcinoma cells were separated on SDS-PAGE and probed with Anti-Cofilin antibody produced in Rabbit. The antibody was developed using 1:6000 Anti-Rabbit IgG (whole molecule)-Alkaline Phosphatase antibody produced in Goat.Lanes:1. 1:10,000 antibody2. 1:20,000 antibody3. Negative Control](http://images.novusbio.com/fullsize/Cofilin-Antibody-Western-Blot-NB100-1900-img0002.jpg "Western Blot: Cofilin Antibody [NB100-1900] - Whole extract of A-431 Human epithelial carcinoma cells were separated on SDS-PAGE and probed with Anti-Cofilin antibody produced in Rabbit. The antibody was developed using 1:6000 Anti-Rabbit IgG (whole molecule)-Alkaline Phosphatase antibody produced in Goat.Lanes:1. 1:10,000 antibody2. 1:20,000 antibody3. Negative Control")
![Immunocytochemistry/Immunofluorescence: Cofilin Antibody [NB100-1900] - NIH-3T3 Mouse fibroblasts cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.2% Triton X-100. Fixed cells were stained with 1:1,000 Anti-Cofilin antibody produced in Rabbit . The antibody was developed using 1:100 Anti-Rabbit IgG (whole molecule)-FITC antibody produced in Goat. Picture B: cells were counterstained with DAPI (blue) to stain nuclei.](http://images.novusbio.com/fullsize/Cofilin-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1900-img0001.jpg)
![Western Blot: Cofilin [p Ser3] Antibody [NBP3-05394] - Western blot analysis of extracts of NIH/3T3 cells, using Cofilin pAb (NBP3-05394) at 1:1000 dilution or CFL1 antibody (A1704).NIH/3T3 cells were treated by CIP(20uL/400ul) at 37C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/Cofilin-[p-Ser3]-Antibody-Western-Blot-NBP3-05394-img0003.jpg "Western Blot: Cofilin [p Ser3] Antibody [NBP3-05394] - Western blot analysis of extracts of NIH/3T3 cells, using Cofilin pAb (NBP3-05394) at 1:1000 dilution or CFL1 antibody (A1704).NIH/3T3 cells were treated by CIP(20uL/400ul) at 37C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 3s.")
![Immunocytochemistry/Immunofluorescence: Cofilin [p Ser3] Antibody [NBP3-05394] - Immunofluorescence analysis of methanol-fixed HeLa cells using Cofilin antibody (NBP3-05394).](http://images.novusbio.com/fullsize/Cofilin-[p-Ser3]-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05394-img0002.jpg)
![Immunohistochemistry-Paraffin: Cofilin [p Ser3] Antibody [NBP3-05394] - Immunohistochemistry of paraffin-embedded human breast carcinoma tissue, using Cofilin antibody (NBP3-05394).](http://images.novusbio.com/fullsize/Cofilin-[p-Ser3]-Antibody-Immunohistochemistry-Paraffin-NBP3-05394-img0001.jpg)
![Western Blot: Cofilin Antibody (S06-2C0) [NBP3-15015] - Western blot detection of Cofilin in A549, MCF-7, 293T cell lysates using Cofilin Rabbit mAb (1:1000 diluted). Predicted band size: 19kDa. Observed band size: 19kDa.](http://images.novusbio.com/fullsize/Cofilin-Antibody-S06-2C0-Western-Blot-NBP3-15015-img0001.jpg "Western Blot: Cofilin Antibody (S06-2C0) [NBP3-15015] - Western blot detection of Cofilin in A549, MCF-7, 293T cell lysates using Cofilin Rabbit mAb (1:1000 diluted). Predicted band size: 19kDa. Observed band size: 19kDa.")
![Immunocytochemistry/Immunofluorescence: Cofilin Antibody (S06-2C0) [NBP3-15015] - Immunocytochemistry of Cofilin (green) in hela using Cofilin Rabbit mAb at dilution 1/50, and DAPI (blue)](http://images.novusbio.com/fullsize/Cofilin-Antibody-S06-2C0-Immunocytochemistry-Immunofluorescence-NBP3-15015-img0002.jpg)
![Immunohistochemistry: Cofilin Antibody (S06-2C0) [NBP3-15015] - Immunohistochemical of Cofilin in Human lung cancer tissue using Cofilin antibody at dilution 1/2](http://images.novusbio.com/fullsize/Cofilin-Antibody-S06-2C0-Immunohistochemistry-NBP3-15015-img0003.jpg)
![Western Blot: Cofilin Antibody (PSH0-33) [NBP3-32204] - Western blot analysis of Cofilin on different lysates with Rabbit anti-Cofilin antibody (NBP3-32204) at 1/1,000 dilution. Lane 1: Hela cell lysate (30 ug/Lane) Lane 2: HEK-293 cell lysate (30 ug/Lane) Lane 3: MCF-7 cell lysate (30 ug/Lane) Lane 4: MDA-MB-468 cell lysate (24 ug/Lane) Lane 5: K-562 cell lysate (16 ug/Lane) Lane 6: SH-SY5Y cell lysate (22 ug/Lane) Lane 7: HUVEC cell lysate (30 ug/Lane) Lane 8: Jurkat cell lysate (30 ug/Lane) Lane 9: A431 cell lysate (20 ug/Lane) Lane 10: COS-1 cell lysate (30 ug/Lane) Lane 11: VERO cell lysate (30 ug/Lane) Lane 12: NIH/3T3 cell lysate (22 ug/Lane) Lane 13: PC-12 cell lysate (30 ug/Lane) Lane 14: Neuro-2a cell lysate (19 ug/Lane) Predicted band size: 19 kDa Observed band size: 19 kDa Exposure time: 20 seconds; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32204) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32204_rabbit-cofilin-mab-psh0-33-27820241631651.jpg "Western Blot: Cofilin Antibody (PSH0-33) [NBP3-32204] - Western blot analysis of Cofilin on different lysates with Rabbit anti-Cofilin antibody (NBP3-32204) at 1/1,000 dilution. Lane 1: Hela cell lysate (30 ug/Lane) Lane 2: HEK-293 cell lysate (30 ug/Lane) Lane 3: MCF-7 cell lysate (30 ug/Lane) Lane 4: MDA-MB-468 cell lysate (24 ug/Lane) Lane 5: K-562 cell lysate (16 ug/Lane) Lane 6: SH-SY5Y cell lysate (22 ug/Lane) Lane 7: HUVEC cell lysate (30 ug/Lane) Lane 8: Jurkat cell lysate (30 ug/Lane) Lane 9: A431 cell lysate (20 ug/Lane) Lane 10: COS-1 cell lysate (30 ug/Lane) Lane 11: VERO cell lysate (30 ug/Lane) Lane 12: NIH/3T3 cell lysate (22 ug/Lane) Lane 13: PC-12 cell lysate (30 ug/Lane) Lane 14: Neuro-2a cell lysate (19 ug/Lane) Predicted band size: 19 kDa Observed band size: 19 kDa Exposure time: 20 seconds; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32204) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: Cofilin Antibody (PSH0-33) [NBP3-32204] - Immunocytochemistry analysis of NIH/3T3 cells labeling Cofilin with Rabbit anti-Cofilin antibody (NBP3-32204) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Cofilin antibody (NBP3-32204) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32204_rabbit-cofilin-mab-psh0-33-29202410334062.jpg)
![Flow Cytometry: Cofilin Antibody (PSH0-33) [NBP3-32204] - Flow cytometric analysis of Hela cells labeling Cofilin. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32204, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32204_rabbit-cofilin-mab-psh0-33-29202416372129.jpg)
![Western Blot: Cofilin Antibody [NB100-81866] - Analysis of extracts from MDA cells using cofilin (Ab-3)](http://images.novusbio.com/fullsize/Cofilin-Antibody-Western-Blot-NB100-81866-img0001.jpg "Western Blot: Cofilin Antibody [NB100-81866] - Analysis of extracts from MDA cells using cofilin (Ab-3)")
![Immunohistochemistry-Paraffin: Cofilin Antibody [NB100-81866] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NB100-81866 (left) or the same antibody preincubated with blocking peptide(right).](http://images.novusbio.com/fullsize/Cofilin-Antibody-Immunohistochemistry-NB100-81866-img0002.jpg)
![Western Blot: Cofilin Antibody (1C1) [NBP1-21044] - The lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Cofilin 1(1:1000) antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: HeLa cell lysateLane 2.: K562 cell lysateLane 3.: MCF7 cell lysateLane 4.: LnCap cell lysateLane 5.: PC3 cell lysate](http://images.novusbio.com/fullsize/Cofilin-Antibody-1C1-Western-Blot-NBP1-21044-img0007.jpg "Western Blot: Cofilin Antibody (1C1) [NBP1-21044] - The lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Cofilin 1(1:1000) antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: HeLa cell lysateLane 2.: K562 cell lysateLane 3.: MCF7 cell lysateLane 4.: LnCap cell lysateLane 5.: PC3 cell lysate")
![Immunocytochemistry/Immunofluorescence: Cofilin Antibody (1C1) [NBP1-21044] - Analysis of Cofilin1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human Cofilin1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/Cofilin-Antibody-1C1-Immunocytochemistry-Immunofluorescence-NBP1-21044-img0006.jpg)
![Western Blot: Cofilin [p Ser3] Antibody [NBP3-23231] - Western blot of Cofilin in jurkat cells. The blots were untreated (lanes 1 & 4) or treated (lanes 2, 3, 5 & 6) with lambda phosphatase. In lanes 3 & 6, the phosphatase was inhibited with phospho-Cofilin (Ser-3) peptide. The blots were probed with anti-Cofilin (Ser-3) phospho-specific (lanes 1-3) or anti-Cofilin (N-terminus) (lanes 4-6).](http://images.novusbio.com/fullsize/nbp3-23231_rabbit-cofilin-p-ser3-pab-199202312403712.jpg "Western Blot: Cofilin [p Ser3] Antibody [NBP3-23231] - Western blot of Cofilin in jurkat cells. The blots were untreated (lanes 1 & 4) or treated (lanes 2, 3, 5 & 6) with lambda phosphatase. In lanes 3 & 6, the phosphatase was inhibited with phospho-Cofilin (Ser-3) peptide. The blots were probed with anti-Cofilin (Ser-3) phospho-specific (lanes 1-3) or anti-Cofilin (N-terminus) (lanes 4-6).")
![Western Blot: Cofilin Antibody [NBP3-23230] - Western blot of Cofilin in jurkat cells. The blots were untreated (lanes 1 & 4) or treated (lanes 2, 3, 5 & 6) with lambda phosphatase. In lanes 3 & 6, the phosphatase was inhibited with phospho-Cofilin (Ser-3) peptide. The blots were probed with anti-Cofilin (Ser-3) phospho-specific (lanes 1-3) or anti-Cofilin (N-terminus) (lanes 4-6).](http://images.novusbio.com/fullsize/nbp3-23230_rabbit-cofilin-pab-19920231759441.jpg "Western Blot: Cofilin Antibody [NBP3-23230] - Western blot of Cofilin in jurkat cells. The blots were untreated (lanes 1 & 4) or treated (lanes 2, 3, 5 & 6) with lambda phosphatase. In lanes 3 & 6, the phosphatase was inhibited with phospho-Cofilin (Ser-3) peptide. The blots were probed with anti-Cofilin (Ser-3) phospho-specific (lanes 1-3) or anti-Cofilin (N-terminus) (lanes 4-6).")