CDK4 Antibodies
We offer CDK4 Antibodies for use in common research applications: ELISA, Immunocytochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Microarray, Proximity Ligation Assay, Sandwich ELISA, Western Blot. Each CDK4 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our CDK4 Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the CDK4 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our CDK4 polyclonal antibodies and browse our CDK4 monoclonal antibody catalog.
Alternate Names for CDK4 Antibodies
anti-CDK4 antibody, anti-Cell division protein kinase 4 antibody, anti-CMM3 antibody, anti-cyclin-dependent kinase 4 antibody, anti-EC 2.7.11 antibody, anti-EC 2.7.11.22 antibody, anti-melanoma cutaneous malignant, 3 antibody, anti-MGC14458 antibody, anti-PSK-J3cell division kinase 4 antibody
17 Results for "cdk4" in Primary Antibodies
![Immunocytochemistry/Immunofluorescence: CDK4 Antibody [NBP1-31308] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CDK4 protein stained by CDK4 antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 um.](http://images.novusbio.com/fullsize/CDK4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31308-img0010.jpg "Immunocytochemistry/Immunofluorescence: CDK4 Antibody [NBP1-31308] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CDK4 protein stained by CDK4 antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 um.")
![Immunohistochemistry-Paraffin: CDK4 Antibody [NBP1-31308] - Human gastric cancer, using CDK4 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Cdk4-Antibody-Immunohistochemistry-Paraffin-NBP1-31308-img0006.jpg)
![Western Blot: CDK4 Antibody [NBP1-31308] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with CDK4 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/CDK4-Antibody-Western-Blot-NBP1-31308-img0012.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: CDK4 Antibody [NBP1-31308] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CDK4 antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/CDK4-Antibody-Western-Blot-NBP1-31308-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: CDK4 Antibody [NBP1-31308] - MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CDK4 protein stained by CDK4 antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/CDK4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31308-img0009.jpg)
![Western Blot: CDK4 Antibody [NBP1-31308] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with CDK4 antibody (NBP1-31308) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31308_rabbit-polyclonal-cdk4-antibody-9102024205643.jpg)
![Western Blot: CDK4 Antibody [NBP1-31308] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with CDK4 antibody (NBP1-31308) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31308_rabbit-polyclonal-cdk4-antibody-9102024209146.jpg)
![Western Blot: CDK4 Antibody [NBP1-31308] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CDK4 antibody (NBP1-31308) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31308_rabbit-polyclonal-cdk4-antibody-910202420104465.jpg)
![Western Blot: CDK4 Antibody [NBP2-32288] - Reccomended Titration: 1.0 ug/ml Positive Control: HepG2 Whole Cell](http://images.novusbio.com/fullsize/CDK4-Antibody-Western-Blot-NBP2-32288-img0018.jpg "Western Blot: CDK4 Antibody [NBP2-32288] - Reccomended Titration: 1.0 ug/ml Positive Control: HepG2 Whole Cell")
![Immunohistochemistry: CDK4 Antibody [NBP2-32288] - Paraffin Embedded Tissue: Human Heart cell Cellular Data: Epithelial cells of renal tubule Antibody Concentration: 4.0 - 8.0 ug/ml Magnification: 400X](http://images.novusbio.com/fullsize/CDK4-Antibody-Immunohistochemistry-NBP2-32288-img0017.jpg)
![Western Blot: CDK4 Antibody [NBP2-32288] - Lanes: 1. 30 ug human HCT116 cell lysate 2. 30 ug genotoxic treated human HCT116 cell lysate 3. 30 ug genotoxic treated human HCT116 cell lysate Primary, Antibody Dilution: 1 : 2000 Secondary Antibody: Anti-rabbit HRP Secondary, Antibody Dilution: 1 : 5000 Gene name: CDK4.](http://images.novusbio.com/fullsize/CDK4-Antibody-Western-Blot-NBP2-32288-img0016.jpg)
![Western Blot: CDK4 Antibody [NBP2-32288] - Western blot analysis of HeLa (A) and K562 (B) cell lysate using Cdk4 antibody (NBP2-32288) at 2ug/ml.](http://images.novusbio.com/fullsize/CDK4-Antibody-Western-Blot-NBP2-32288-img0019.jpg)
![Western Blot: CDK4 Antibody (3G7Q7) [NBP3-15344] - Western blot analysis of extracts of MCF7 cells, using CDK4 Rabbit mAb (NBP3-15344) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/CDK4-Antibody-3G7Q7-Western-Blot-NBP3-15344-img0008.jpg "Western Blot: CDK4 Antibody (3G7Q7) [NBP3-15344] - Western blot analysis of extracts of MCF7 cells, using CDK4 Rabbit mAb (NBP3-15344) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunocytochemistry/Immunofluorescence: CDK4 Antibody (3G7Q7) [NBP3-15344] - Immunofluorescence analysis of HeLa cells using CDK4 Rabbit mAb (NBP3-15344) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/CDK4-Antibody-3G7Q7-Immunocytochemistry-Immunofluorescence-NBP3-15344-img0007.jpg)
![Immunohistochemistry-Paraffin: CDK4 Antibody (3G7Q7) [NBP3-15344] - Immunohistochemistry of paraffin-embedded rat ovary using [KO Validated] CDK4 Rabbit mAb (NBP3-15344) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CDK4-Antibody-3G7Q7-Immunohistochemistry-Paraffin-NBP3-15344-img0006.jpg)
![Immunohistochemistry-Paraffin: CDK4 Antibody (3G7Q7) [NBP3-15344] - Immunohistochemistry of paraffin-embedded human breast cancer using [KO Validated] CDK4 Rabbit mAb (NBP3-15344) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CDK4-Antibody-3G7Q7-Immunohistochemistry-Paraffin-NBP3-15344-img0001.jpg)
![Immunohistochemistry-Paraffin: CDK4 Antibody (3G7Q7) [NBP3-15344] - Immunohistochemistry of paraffin-embedded human colon carcinoma using [KO Validated] CDK4 Rabbit mAb (NBP3-15344) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CDK4-Antibody-3G7Q7-Immunohistochemistry-Paraffin-NBP3-15344-img0002.jpg)
![Immunohistochemistry-Paraffin: CDK4 Antibody (3G7Q7) [NBP3-15344] - Immunohistochemistry of paraffin-embedded mouse lung using [KO Validated] CDK4 Rabbit mAb (NBP3-15344) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CDK4-Antibody-3G7Q7-Immunohistochemistry-Paraffin-NBP3-15344-img0003.jpg)
![Immunohistochemistry-Paraffin: CDK4 Antibody (3G7Q7) [NBP3-15344] - Immunohistochemistry of paraffin-embedded mouse spleen using [KO Validated] CDK4 Rabbit mAb (NBP3-15344) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CDK4-Antibody-3G7Q7-Immunohistochemistry-Paraffin-NBP3-15344-img0004.jpg)
![Immunohistochemistry-Paraffin: CDK4 Antibody (3G7Q7) [NBP3-15344] - Immunohistochemistry of paraffin-embedded rat lung using [KO Validated] CDK4 Rabbit mAb (NBP3-15344) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CDK4-Antibody-3G7Q7-Immunohistochemistry-Paraffin-NBP3-15344-img0005.jpg)
![Western Blot: CDK4 Antibody (6N3M6) [CDK4] - Western blot analysis of various lysates, using CDK4 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-15344_rabbit-cdk4-mab-6n3m6-52202517162385.jpg)
![Immunohistochemistry: CDK4 Antibody (6N3M6) [CDK4] - Immunohistochemistry analysis of paraffin-embedded Human prostate tissue using [KO Validated] CDK4 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15344_rabbit-cdk4-mab-6n3m6-52202517284930.jpg)
![Immunohistochemistry: CDK4 Antibody (6N3M6) [NBP3-15344] - Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using [KO Validated] CDK4 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15344_rabbit-cdk4-mab-6n3m6-72202512435398.jpg)
![Immunohistochemistry: CDK4 Antibody (6N3M6) [NBP3-15344] - Immunohistochemistry analysis of paraffin-embedded Human cervix cancer tissue using [KO Validated] CDK4 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15344_rabbit-cdk4-mab-6n3m6-7220251245317.jpg)
![Immunohistochemistry: CDK4 Antibody (6N3M6) [NBP3-15344] - Immunohistochemistry analysis of paraffin-embedded Human cervix cancer tissue using [KO Validated] CDK4 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15344_rabbit-cdk4-mab-6n3m6-7220251245373.jpg)
![Immunohistochemistry: CDK4 Antibody (6N3M6) [NBP3-15344] - Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using [KO Validated] CDK4 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15344_rabbit-cdk4-mab-6n3m6-7220251245387.jpg)
![Western Blot: CDK4 Antibody (CL8102) [NBP2-88912] - Analysis in human cell line RH-30.](http://images.novusbio.com/fullsize/CDK4-Antibody-CL8102-Western-Blot-NBP2-88912-img0007.jpg "Western Blot: CDK4 Antibody (CL8102) [NBP2-88912] - Analysis in human cell line RH-30.")
![Immunocytochemistry/Immunofluorescence: CDK4 Antibody (CL8102) [NBP2-88912] - Staining of RH-30 cells using the Anti-CDK4 monoclonal antibody, showing specific staining in the nucleoplasm and cytosol in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/CDK4-Antibody-CL8102-Immunocytochemistry-Immunofluorescence-NBP2-88912-img0001.jpg)
![Immunohistochemistry-Paraffin: CDK4 Antibody (CL8102) [NBP2-88912] - Staining of human skeletal muscle shows moderate nuclear positivity in myocytes.](http://images.novusbio.com/fullsize/CDK4-Antibody-CL8102-Immunohistochemistry-Paraffin-NBP2-88912-img0006.jpg)
![Immunohistochemistry-Paraffin: CDK4 Antibody (CL8102) [NBP2-88912] - Staining of human cervix, uterine shows moderate to strong nuclear positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/CDK4-Antibody-CL8102-Immunohistochemistry-Paraffin-NBP2-88912-img0002.jpg)
![Immunohistochemistry-Paraffin: CDK4 Antibody (CL8102) [NBP2-88912] - Staining of human endometrium shows strong nuclear positivity in cells in endometrial stroma.](http://images.novusbio.com/fullsize/CDK4-Antibody-CL8102-Immunohistochemistry-Paraffin-NBP2-88912-img0003.jpg)
![Immunohistochemistry-Paraffin: CDK4 Antibody (CL8102) [NBP2-88912] - Staining of human liver cancer shows strong nuclear positivity in tumor cells.](http://images.novusbio.com/fullsize/CDK4-Antibody-CL8102-Immunohistochemistry-Paraffin-NBP2-88912-img0004.jpg)
![Immunohistochemistry-Paraffin: CDK4 Antibody (CL8102) [NBP2-88912] - Staining of human prostate shows strong nuclear positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/CDK4-Antibody-CL8102-Immunohistochemistry-Paraffin-NBP2-88912-img0005.jpg)
![Western Blot: CDK4 Antibody (4F11) [H00001019-M03] - Analysis of CDK4 expression in transfected 293T cell line by CDK4 monoclonal antibody (M03), clone 4F11. Lane 1: CDK4 transfected lysatE (33.7 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/CDK4-Antibody-4F11-Western-Blot-H00001019-M03-img0012.jpg "Western Blot: CDK4 Antibody (4F11) [H00001019-M03] - Analysis of CDK4 expression in transfected 293T cell line by CDK4 monoclonal antibody (M03), clone 4F11. Lane 1: CDK4 transfected lysatE (33.7 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: CDK4 Antibody (4F11) [H00001019-M03] - Analysis of monoclonal antibody to CDK4 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/CDK4-Antibody-4F11-Immunocytochemistry-Immunofluorescence-H00001019-M03-img0009.jpg)
![Western Blot: CDK4 Antibody (4F11) [H00001019-M03] - Western Blot analysis of CDK4 expression in HeLa ( Cat # L013V1 ).](http://images.novusbio.com/fullsize/CDK4-Antibody-4F11-Western-Blot-H00001019-M03-img0001.jpg)
![Western Blot: CDK4 Antibody (4F11) [H00001019-M03] - Western Blot analysis of CDK4 expression in Raw 264.7 ( Cat # L024V1 ).](http://images.novusbio.com/fullsize/CDK4-Antibody-4F11-Western-Blot-H00001019-M03-img0002.jpg)
![Western Blot: CDK4 Antibody (4F11) [H00001019-M03] - Western Blot analysis of CDK4 expression in NIH/3T3 ( Cat # L018V1 ).](http://images.novusbio.com/fullsize/CDK4-Antibody-4F11-Western-Blot-H00001019-M03-img0005.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: CDK4 Antibody (4F11) [H00001019-M03] - Western blot analysis of CDK4 over-expressed 293 cell line. Lane 2 cotransfected with CDK4 Validated Chimera RNAi or non-transfected control. Blot probed with H00001019-M03. GAPDH (36.1 kDa) used as loading control.](http://images.novusbio.com/fullsize/CDK4-Antibody-4F11-Western-Blot-H00001019-M03-img0007.jpg)
![Western Blot: CDK4 Antibody (4F11) [H00001019-M03] - Analysis of CDK4 expression in 293.](http://images.novusbio.com/fullsize/CDK4-Antibody-4F11-Western-Blot-H00001019-M03-img0010.jpg)
![Western Blot: CDK4 Antibody (4F11) [H00001019-M03] - Analysis of CDK4 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/CDK4-Antibody-4F11-Western-Blot-H00001019-M03-img0011.jpg)
![ELISA: CDK4 Antibody (4F11) [H00001019-M03] - Detection limit for recombinant GST tagged CDK4 is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/CDK4-Antibody-4F11-ELISA-H00001019-M03-img0008.jpg)
![Western Blot: CDK4 Antibody (DCS-31) [NB120-6315] - WB analysis of lysates from human HeLa and SKUT cells using CDK4 antibody (clone DCS-31).](http://images.novusbio.com/fullsize/CDK4-Antibody-DCS-31-Western-Blot-NB120-6315-img0001.jpg "Western Blot: CDK4 Antibody (DCS-31) [NB120-6315] - WB analysis of lysates from human HeLa and SKUT cells using CDK4 antibody (clone DCS-31).")
![Western Blot: CDK4 Antibody (2G7) [H00001019-M04] - Analysis of CDK4 expression in NIH/3T3 (Cat # L018V1).](http://images.novusbio.com/fullsize/CDK4-Antibody-2G7-Western-Blot-H00001019-M04-img0009.jpg "Western Blot: CDK4 Antibody (2G7) [H00001019-M04] - Analysis of CDK4 expression in NIH/3T3 (Cat # L018V1).")
![Immunocytochemistry/Immunofluorescence: CDK4 Antibody (2G7) [H00001019-M04] - Analysis of monoclonal antibody to CDK4 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/CDK4-Antibody-2G7-Immunocytochemistry-Immunofluorescence-H00001019-M04-img0006.jpg)
![Western Blot: CDK4 Antibody (2G7) [H00001019-M04] - Analysis of CDK4 expression in HeLa (Cat # L013V1).](http://images.novusbio.com/fullsize/CDK4-Antibody-2G7-Western-Blot-H00001019-M04-img0007.jpg)
![Western Blot: CDK4 Antibody (2G7) [H00001019-M04] - Analysis of CDK4 expression in Raw 264.7 (Cat # L024V1).](http://images.novusbio.com/fullsize/CDK4-Antibody-2G7-Western-Blot-H00001019-M04-img0008.jpg)
![ELISA: CDK4 Antibody (2G7) [H00001019-M04] - Detection limit for recombinant GST tagged CDK4 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/CDK4-Antibody-2G7-ELISA-H00001019-M04-img0005.jpg)
![Western Blot: CDK4 Antibody (8A10) [H00001019-M09] - Analysis of CDK4 expression in NIH/3T3 (Cat # L018V1).](http://images.novusbio.com/fullsize/CDK4-Antibody-8A10-Western-Blot-H00001019-M09-img0010.jpg "Western Blot: CDK4 Antibody (8A10) [H00001019-M09] - Analysis of CDK4 expression in NIH/3T3 (Cat # L018V1).")
![Immunocytochemistry/Immunofluorescence: CDK4 Antibody (8A10) [H00001019-M09] - Analysis of monoclonal antibody to CDK4 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/CDK4-Antibody-8A10-Immunocytochemistry-Immunofluorescence-H00001019-M09-img0007.jpg)
![Western Blot: CDK4 Antibody (8A10) [H00001019-M09] - Analysis of CDK4 expression in HeLa (Cat # L013V1).](http://images.novusbio.com/fullsize/CDK4-Antibody-8A10-Western-Blot-H00001019-M09-img0008.jpg)
![Western Blot: CDK4 Antibody (8A10) [H00001019-M09] - Analysis of CDK4 expression in Raw 264.7 (Cat # L024V1).](http://images.novusbio.com/fullsize/CDK4-Antibody-8A10-Western-Blot-H00001019-M09-img0009.jpg)
![ELISA: CDK4 Antibody (8A10) [H00001019-M09] - Detection limit for recombinant GST tagged CDK4 is approximately 3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/CDK4-Antibody-8A10-ELISA-H00001019-M09-img0005.jpg)
![Immunoprecipitation: CDK4 Antibody (8F2) [NBP3-26664] - Immunoprecipitating CDK4 in Hela whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26664 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/1500). Lane 2:NBP3-26664 (2ug)+ Hela whole cell lysate (500ug). Lane 3: Hela whole cell lysate (10ug).](http://images.novusbio.com/fullsize/nbp3-26664_rabbit-cdk4-mab-8f2-262202413221518.jpg "Immunoprecipitation: CDK4 Antibody (8F2) [NBP3-26664] - Immunoprecipitating CDK4 in Hela whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26664 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/1500). Lane 2:NBP3-26664 (2ug)+ Hela whole cell lysate (500ug). Lane 3: Hela whole cell lysate (10ug).")
![Western Blot: CDK4 Antibody (8F2) [NBP3-26664] - Positive Western Blot detected in: Hela whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate, MCF-7 whole cell lysate, PC-3 whole cell lysate. All lanes: CDK4 Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 34, 21 kDa Observed band size: 34 kDa](http://images.novusbio.com/fullsize/nbp3-26664_rabbit-cdk4-mab-8f2-28220241140923.jpg)
![Western Blot: CDK4 Antibody (5-C3-G3-R) [NBP3-32175] - Western blot analysis of CDK4 on different lysates with Mouse anti-CDK4 antibody (NBP3-32175) at 1/1,000 dilution. Lane 1: A549 cell lysate Lane 2: 293T cell lysate Lane 3: HeLa cell lysate Lane 4: MCF7 cell lysate Lane 5: K-562 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 34 kDa Observed band size: 34 kDa Exposure time: 17 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32175) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32175_mouse-cdk4-mab-5-c3-g3-r-2782024163165.jpg "Western Blot: CDK4 Antibody (5-C3-G3-R) [NBP3-32175] - Western blot analysis of CDK4 on different lysates with Mouse anti-CDK4 antibody (NBP3-32175) at 1/1,000 dilution. Lane 1: A549 cell lysate Lane 2: 293T cell lysate Lane 3: HeLa cell lysate Lane 4: MCF7 cell lysate Lane 5: K-562 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 34 kDa Observed band size: 34 kDa Exposure time: 17 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32175) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: CDK4 Antibody [NBP2-58877] - Staining of human cell line MCF7 shows localization to nucleus, nucleoli, nuclear membrane & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/CDK4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58877-img0001.jpg "Immunocytochemistry/Immunofluorescence: CDK4 Antibody [NBP2-58877] - Staining of human cell line MCF7 shows localization to nucleus, nucleoli, nuclear membrane & cytosol. Antibody staining is shown in green.")