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CDK4 Antibodies

We offer CDK4 Antibodies for use in common research applications: ELISA, Immunocytochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Microarray, Proximity Ligation Assay, Sandwich ELISA, Western Blot. Each CDK4 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our CDK4 Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the CDK4 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our CDK4 polyclonal antibodies and browse our CDK4 monoclonal antibody catalog.

Alternate Names for CDK4 Antibodies

anti-CDK4 antibody, anti-Cell division protein kinase 4 antibody, anti-CMM3 antibody, anti-cyclin-dependent kinase 4 antibody, anti-EC 2.7.11 antibody, anti-EC 2.7.11.22 antibody, anti-melanoma cutaneous malignant, 3 antibody, anti-MGC14458 antibody, anti-PSK-J3cell division kinase 4 antibody

17 Results for "cdk4" in Primary Antibodies

NBP1-31308

CDK4 Antibody

(7 Publications)

100 ul

NBP1-31308

Immunocytochemistry/Immunofluorescence: CDK4 Antibody [NBP1-31308] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CDK4 protein stained by CDK4 antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 um.

Immunohistochemistry-Paraffin: CDK4 Antibody [NBP1-31308] - Human gastric cancer, using CDK4 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western Blot: CDK4 Antibody [NBP1-31308] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with CDK4 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

<b>Genetic Strategies Validation. </b>Western Blot: CDK4 Antibody [NBP1-31308] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CDK4 antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: CDK4 Antibody [NBP1-31308] - MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CDK4 protein stained by CDK4 antibody diluted at 1:1000.

Western Blot: CDK4 Antibody [NBP1-31308] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with CDK4 antibody (NBP1-31308) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: CDK4 Antibody [NBP1-31308] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with CDK4 antibody (NBP1-31308) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: CDK4 Antibody [NBP1-31308] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CDK4 antibody (NBP1-31308) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Reactivity:

Hu, Mu, Rt, Bv, Po, RM, Sh

Species Glossary

Reactivity: Hu, Mu, Rt, Bv, Po, RM, Sh

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP2-32288

CDK4 Antibody

(1 Publication)

100 ul

NBP2-32288

Western Blot: CDK4 Antibody [NBP2-32288] - Reccomended Titration: 1.0 ug/ml Positive Control: HepG2 Whole Cell

Immunohistochemistry: CDK4 Antibody [NBP2-32288] - Paraffin Embedded Tissue: Human Heart cell Cellular Data: Epithelial cells of renal tubule Antibody Concentration: 4.0 - 8.0 ug/ml Magnification: 400X

Western Blot: CDK4 Antibody [NBP2-32288] - Lanes: 1. 30 ug human HCT116 cell lysate 2. 30 ug genotoxic treated human HCT116 cell lysate 3. 30 ug genotoxic treated human HCT116 cell lysate Primary, Antibody Dilution: 1 : 2000 Secondary Antibody: Anti-rabbit HRP Secondary, Antibody Dilution: 1 : 5000 Gene name: CDK4.

Western Blot: CDK4 Antibody [NBP2-32288] - Western blot analysis of HeLa (A) and K562 (B) cell lysate using Cdk4 antibody (NBP2-32288) at 2ug/ml.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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AF5254

CDK4 Antibody [Unconjugated]

(2 Publications)

100 μg

AF5254

Western blot shows lysates of A431 human epithelial carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse CDK4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5254) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for CDK4 at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

CDK4 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human/Mouse CDK4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5254) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

CDK4 was detected in immersion fixed RAW264.7 cells using Goat Anti-Human/Mouse CDK4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5254) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cells, and MCF‑7 human breast cancer cells, loaded at 0.2 mg/mL. A specific band was detected for CDK4 at approximately 38 kDa (as indicated) using 20 µg/mL of Goat Anti-Human/Mouse CDK4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5254). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, ,

Applications: , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF5254-SP

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NBP3-15344

CDK4 Antibody (6N3M6)

100 ul

NBP3-15344

Western Blot: CDK4 Antibody (3G7Q7) [NBP3-15344] - Western blot analysis of extracts of MCF7 cells, using CDK4 Rabbit mAb (NBP3-15344) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Immunocytochemistry/Immunofluorescence: CDK4 Antibody (3G7Q7) [NBP3-15344] - Immunofluorescence analysis of HeLa cells using CDK4 Rabbit mAb (NBP3-15344) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunohistochemistry-Paraffin: CDK4 Antibody (3G7Q7) [NBP3-15344] - Immunohistochemistry of paraffin-embedded rat ovary using [KO Validated] CDK4 Rabbit mAb (NBP3-15344) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry-Paraffin: CDK4 Antibody (3G7Q7) [NBP3-15344] - Immunohistochemistry of paraffin-embedded human breast cancer using [KO Validated] CDK4 Rabbit mAb (NBP3-15344) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry-Paraffin: CDK4 Antibody (3G7Q7) [NBP3-15344] - Immunohistochemistry of paraffin-embedded human colon carcinoma using [KO Validated] CDK4 Rabbit mAb (NBP3-15344) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry-Paraffin: CDK4 Antibody (3G7Q7) [NBP3-15344] - Immunohistochemistry of paraffin-embedded mouse lung using [KO Validated] CDK4 Rabbit mAb (NBP3-15344) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry-Paraffin: CDK4 Antibody (3G7Q7) [NBP3-15344] - Immunohistochemistry of paraffin-embedded mouse spleen using [KO Validated] CDK4 Rabbit mAb (NBP3-15344) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry-Paraffin: CDK4 Antibody (3G7Q7) [NBP3-15344] - Immunohistochemistry of paraffin-embedded rat lung using [KO Validated] CDK4 Rabbit mAb (NBP3-15344) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Western Blot: CDK4 Antibody (6N3M6) [CDK4] - Western blot analysis of various lysates, using CDK4 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.

Immunohistochemistry: CDK4 Antibody (6N3M6) [CDK4] - Immunohistochemistry analysis of paraffin-embedded Human prostate tissue using [KO Validated] CDK4 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry: CDK4 Antibody (6N3M6) [NBP3-15344] - Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using [KO Validated] CDK4 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry: CDK4 Antibody (6N3M6) [NBP3-15344] - Immunohistochemistry analysis of paraffin-embedded Human cervix cancer tissue using [KO Validated] CDK4 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry: CDK4 Antibody (6N3M6) [NBP3-15344] - Immunohistochemistry analysis of paraffin-embedded Human cervix cancer tissue using [KO Validated] CDK4 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry: CDK4 Antibody (6N3M6) [NBP3-15344] - Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using [KO Validated] CDK4 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

,

Applications: ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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NBP2-88912

CDK4 Antibody (CL8102)

100 ul

NBP2-88912

Western Blot: CDK4 Antibody (CL8102) [NBP2-88912] - Analysis in human cell line RH-30.

Immunocytochemistry/Immunofluorescence: CDK4 Antibody (CL8102) [NBP2-88912] - Staining of RH-30 cells using the Anti-CDK4 monoclonal antibody, showing specific staining in the nucleoplasm and cytosol in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Immunohistochemistry-Paraffin: CDK4 Antibody (CL8102) [NBP2-88912] - Staining of human skeletal muscle shows moderate nuclear positivity in myocytes.

Immunohistochemistry-Paraffin: CDK4 Antibody (CL8102) [NBP2-88912] - Staining of human cervix, uterine shows moderate to strong nuclear positivity in squamous epithelial cells.

Immunohistochemistry-Paraffin: CDK4 Antibody (CL8102) [NBP2-88912] - Staining of human endometrium shows strong nuclear positivity in cells in endometrial stroma.

Immunohistochemistry-Paraffin: CDK4 Antibody (CL8102) [NBP2-88912] - Staining of human liver cancer shows strong nuclear positivity in tumor cells.

Immunohistochemistry-Paraffin: CDK4 Antibody (CL8102) [NBP2-88912] - Staining of human prostate shows strong nuclear positivity in smooth muscle cells.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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H00001019-M03

CDK4 Antibody (4F11)

0.1 mg

H00001019-M03

Western Blot: CDK4 Antibody (4F11) [H00001019-M03] - Analysis of CDK4 expression in transfected 293T cell line by CDK4 monoclonal antibody (M03), clone 4F11. Lane 1: CDK4 transfected lysatE (33.7 KDa). Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: CDK4 Antibody (4F11) [H00001019-M03] - Analysis of monoclonal antibody to CDK4 on HeLa cell. Antibody concentration 10 ug/ml

Western Blot: CDK4 Antibody (4F11) [H00001019-M03] - Western Blot analysis of CDK4 expression in HeLa ( Cat # L013V1 ).

Western Blot: CDK4 Antibody (4F11) [H00001019-M03] - Western Blot analysis of CDK4 expression in Raw 264.7 ( Cat # L024V1 ).

Western Blot: CDK4 Antibody (4F11) [H00001019-M03] - Western Blot analysis of CDK4 expression in NIH/3T3 ( Cat # L018V1 ).

<b>Genetic Strategies Validation. </b>Western Blot: CDK4 Antibody (4F11) [H00001019-M03] - Western blot analysis of CDK4 over-expressed 293 cell line. Lane 2 cotransfected with CDK4 Validated Chimera RNAi or non-transfected control. Blot probed with H00001019-M03. GAPDH (36.1 kDa) used as loading control.

Western Blot: CDK4 Antibody (4F11) [H00001019-M03] - Analysis of CDK4 expression in 293.

Western Blot: CDK4 Antibody (4F11) [H00001019-M03] - Analysis of CDK4 expression in Hela S3 NE.

ELISA: CDK4 Antibody (4F11) [H00001019-M03] - Detection limit for recombinant GST tagged CDK4 is approximately 1ng/ml as a capture antibody.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NB120-6315

CDK4 Antibody (DCS-31)

(2 Publications)

0.25 ml

NB120-6315

Western Blot: CDK4 Antibody (DCS-31) [NB120-6315] - WB analysis of lysates from human HeLa and SKUT cells using CDK4 antibody (clone DCS-31).

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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H00001019-M04

CDK4 Antibody (2G7)

0.1 mg

H00001019-M04

Western Blot: CDK4 Antibody (2G7) [H00001019-M04] - Analysis of CDK4 expression in NIH/3T3 (Cat # L018V1).

Immunocytochemistry/Immunofluorescence: CDK4 Antibody (2G7) [H00001019-M04] - Analysis of monoclonal antibody to CDK4 on HeLa cell. Antibody concentration 10 ug/ml

Western Blot: CDK4 Antibody (2G7) [H00001019-M04] - Analysis of CDK4 expression in HeLa (Cat # L013V1).

Western Blot: CDK4 Antibody (2G7) [H00001019-M04] - Analysis of CDK4 expression in Raw 264.7 (Cat # L024V1).

ELISA: CDK4 Antibody (2G7) [H00001019-M04] - Detection limit for recombinant GST tagged CDK4 is approximately 0.1ng/ml as a capture antibody.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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H00001019-M09

CDK4 Antibody (8A10)

0.1 mg

H00001019-M09

Western Blot: CDK4 Antibody (8A10) [H00001019-M09] - Analysis of CDK4 expression in NIH/3T3 (Cat # L018V1).

Immunocytochemistry/Immunofluorescence: CDK4 Antibody (8A10) [H00001019-M09] - Analysis of monoclonal antibody to CDK4 on HeLa cell. Antibody concentration 10 ug/ml

Western Blot: CDK4 Antibody (8A10) [H00001019-M09] - Analysis of CDK4 expression in HeLa (Cat # L013V1).

Western Blot: CDK4 Antibody (8A10) [H00001019-M09] - Analysis of CDK4 expression in Raw 264.7 (Cat # L024V1).

ELISA: CDK4 Antibody (8A10) [H00001019-M09] - Detection limit for recombinant GST tagged CDK4 is approximately 3ng/ml as a capture antibody.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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NBP3-26664

CDK4 Antibody (8F2)

100 ul

NBP3-26664

Immunoprecipitation: CDK4 Antibody (8F2) [NBP3-26664] - Immunoprecipitating CDK4 in Hela whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26664 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/1500). Lane 2:NBP3-26664 (2ug)+ Hela whole cell lysate (500ug). Lane 3: Hela whole cell lysate (10ug).

Western Blot: CDK4 Antibody (8F2) [NBP3-26664] - Positive Western Blot detected in: Hela whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate, MCF-7 whole cell lysate, PC-3 whole cell lysate. All lanes: CDK4 Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 34, 21 kDa Observed band size: 34 kDa

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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NBP3-32175

CDK4 Antibody (5-C3-G3-R)

100 ul

NBP3-32175

Western Blot: CDK4 Antibody (5-C3-G3-R) [NBP3-32175] - Western blot analysis of CDK4 on different lysates with Mouse anti-CDK4 antibody (NBP3-32175) at 1/1,000 dilution. Lane 1: A549 cell lysate Lane 2: 293T cell lysate Lane 3: HeLa cell lysate Lane 4: MCF7 cell lysate Lane 5: K-562 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 34 kDa Observed band size: 34 kDa Exposure time: 17 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32175) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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NBP2-58877

CDK4 Antibody

100 ul

NBP2-58877

Immunocytochemistry/Immunofluorescence: CDK4 Antibody [NBP2-58877] - Staining of human cell line MCF7 shows localization to nucleus, nucleoli, nuclear membrane & cytosol. Antibody staining is shown in green.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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