Bcl-2 Antibodies
We offer Bcl-2 Antibodies for use in common research applications: ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Neutralization, Peptide ELISA, Simple Western, Western Blot. Each Bcl-2 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our Bcl-2 Antibodies can be used in a variety of model species: Bovine, Canine, Feline, Human, Monkey, Mouse, Porcine, Primate, Rat. Use the list below to choose the Bcl-2 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our Bcl-2 polyclonal antibodies and browse our Bcl-2 monoclonal antibody catalog.
Alternate Names for Bcl-2 Antibodies
anti-Bcl-2 antibody, anti-BCL2 antibody, anti-apoptosis regulator Bcl-2 antibody, anti-B-cell CLL/lymphoma 2 antibody
43 Results for "bcl-2" in Primary Antibodies
![Western Blot: Bcl-2 Antibody [NB100-56098] - Analysis of Bcl-2 in whole cell lysate from Daoy cells. Cells were transfected with (1) scrambled control siRNA or (2) Bcl-2 siRNA. Image from verified customer review.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-Western-Blot-NB100-56098-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Bcl-2 Antibody [NB100-56098] - Analysis of Bcl-2 in whole cell lysate from Daoy cells. Cells were transfected with (1) scrambled control siRNA or (2) Bcl-2 siRNA. Image from verified customer review.")
![<b>Genetic Strategies Validation. </b>Western Blot: Bcl-2 Antibody [NB100-56098] - EA reduced MPP+-induced dopaminergic neuronal apoptosis by increasing BDNF (brain-derived neurotrophic factor) expression and further Akt phosphorylation in the rat substantia nigra. Eight days after MPP+ administration, our Western blot results (MAB7566) show that MPP+ treatment reduced tyrosine hydroxylase and Bcl-2 expression in the ipsilateral side of the rat substantia nigra (SN), but not in the contralateral side. EA stimulation (50 Hz) enhanced mature BDNF, tyrosine hydroxylase, and Bcl-2 expression in the MPP+-treated ipsilateral side. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/1422-0067/18/9/1846), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-Western-Blot-NB100-56098-img0012.jpg)
![Immunohistochemistry-Paraffin: Bcl-2 Antibody [NB100-56098] - Analysis of a FFPE human breast carcinoma tissue section using 1:1000 dilution of Bcl-2 antibody (NB100-56098) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). This antibody generated a diffused cytoplasmic staining of BCL2 in the cancer cells with relatively intense signal in their nuclear membranes. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-Immunohistochemistry-Paraffin-NB100-56098-img0011.jpg)
![Western Blot: Bcl-2 Antibody [NB100-56098] - Analysis of Bcl-2 using this antibody at 1:2000, 20 ug/protein was loaded per lane. (1) Human Jurkat T cells. (2) Human RS11 lymphoma cells. (3-6) Human breast cancer cases. Patient sample in lane 5 lacked detectible Bcl-2 expression.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-Western-Blot-NB100-56098-img0005.jpg)
![Western Blot: Bcl-2 Antibody [NB100-56098] - Analysis of Bcl-2 in 293T cell lysate (30ug) using anti-Bcl-2 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-Western-Blot-NB100-56098-img0006.jpg)
![Western Blot: Bcl-2 Antibody [NB100-56098] - Analysis of whole cell lysate (WCL) of Jurkat cells using anti-Bcl-2 antibody (NB100-56098) at 1:1000 dilution. HRP conjugated goat anti-rabbit IgG (H+L) cross adsorbed secondary antibody was used with ECL substrate for the detection of Bcl2 antibody bound to the blotted protein. This Bcl2 antibody detected a specific band at expected molecular weight marker position of Bcl2 protein (26kDa).](http://images.novusbio.com/fullsize/Bcl-2-Antibody-Western-Blot-NB100-56098-img0007.jpg)
![Immunohistochemistry-Paraffin: Bcl-2 Antibody [NB100-56098] - Analysis of a FFPE human breast carcinoma tissue section using 1:1000 dilution of Bcl-2 antibody (NB100-56098) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). This antibody generated a diffused cytoplasmic staining of BCL2 in the cancer cells with relatively intense signal in their nuclear membranes. The endothelial cells and the stromal cells showed a weak signal while the RBCs were negative for BCL2.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-Immunohistochemistry-Paraffin-NB100-56098-img0010.jpg)
![Cholesterol reversed metformin-mediated anticancer potential.Cells were treated with metformin (2 mM) alone and in combination with metformin (2 mM) plus cholesterol (10 μg/ml). (A) Cell viability (MTT) assay was performed to check the reverse effect of cholesterol on metformin- inhibited cell viability. Bars are representing the relative cell viability after 24 hr of treatment. Values represent mean ± SEM of triplicate measurements, **p < .001 vs. control and *p < .001 vs. metformin. (B) Scratch assay was performed. MDA-MB-231 cell monolayers were scratched and incubated with metformin (2 mM) alone and in combination with metformin (2 mM) plus cholesterol (10 μg/ml). The cell monolayers were photographed, and represented photos at 0 hr and 24 hr for control, metformin and metformin plus cholesterol cells were shown here. (C) The unfilled gap areas between two ends of the scratch were measured and subsequently plotted. Values represent mean ± SEM of triplicate measurements, **p < .01 vs. control at 0 hr and **p < .01 vs. metformin at 24 hr. (D) Colony formation assay was performed. After 24 hr of cell seeding, cells were treated with metformin (1mM) alone and in combination with metformin (1mM) plus cholesterol (5 μg/ml). After 5 days, formed colonies were stained with crystal violet. Photos of the wells were taken by a camera. (E) Soft agar assay of MDA-MB-231 cells in presence and absence of metformin (1mM) alone and in combination with metformin (1mM) plus cholesterol (5 μg/ml). Day 9 pictures were shown in the figure. Arrows depict the spheres. (F, G and H) RT-PCR analysis was performed using total RNA isolated from metformin, metformin (1mM) plus cholesterol treated and untreated cells, and gene specific primers. Cholesterol treatment reverses the metformin inhibited genes expressions (Bcl-xL [F], Zeb1 [G] and BMI1 [H]) (compare lane 2 vs. lane 3). (I and J) Western blot analysis was performed using total proteins from metformin, metformin (1mM) plus cholesterol treated and untreated cells, and specific antibodies. Cholesterol treatment reverses the metformin inhibited genes expression (BCL2 [I] and vimentin [J]) (compare lane 2 vs. lane 3). (K) Gelatin zymography was performed using equal amount of conditioned medium of metformin, metformin (2 mM) plus cholesterol (10 μg/ml) treated and untreated cells. Metformin-inhibited MMP activity was increased by treatment of cholesterol (compare lane 2 vs. lane 3). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30625181), licensed under a CC-BY license. Not internally tested by R&D Systems.](http://images.novusbio.com/fullsize/mab8272_human-mouse-rat-bcl-2-mab-clone-625509-41202412513831.jpg)
![Western Blot: Bcl-2 Antibody [NB100-56101] - Analysis of Bcl-2 in 293T cell lysate (30ug) using anti-Bcl-2 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-Western-Blot-NB100-56101-img0002.jpg "Western Blot: Bcl-2 Antibody [NB100-56101] - Analysis of Bcl-2 in 293T cell lysate (30ug) using anti-Bcl-2 antibody. Image from verified customer review.")
![Western Blot: Bcl-2 Antibody (100/D5) [NBP2-15200] - Western Blot Analysis of human MCF-7 cell lysate using Bcl-2 Antibody (100/D5).](http://images.novusbio.com/fullsize/Bcl-2-Antibody-100-D5-Western-Blot-NBP2-15200-img0006.jpg "Western Blot: Bcl-2 Antibody (100/D5) [NBP2-15200] - Western Blot Analysis of human MCF-7 cell lysate using Bcl-2 Antibody (100/D5).")
![Immunohistochemistry-Paraffin: Bcl-2 Antibody (100/D5) [NBP2-15200] - Formalin-paraffin non-Hodgkin's lymphoma stained with Bcl-2 Ab (100/D5). Note nuclear membrane & cytoplasmic staining.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-100-D5-Immunohistochemistry-Paraffin-NBP2-15200-img0005.jpg)
![Flow Cytometry: Bcl-2 Antibody (100/D5) [NBP2-15200] - Intracellular staining of Bcl-2 in fixed and permeabilized Jurkat cells using Bcl-2 antibody (red) and isotype control (green) at 0.5 ug/10^6 cells.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-100-D5-Flow-Cytometry-NBP2-15200-img0002.jpg)
![Western Blot: Bcl-2 Antibody (100/D5) [NBP2-15200] - analysis of Bcl-2 in human skin using Bcl-2 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-100-D5-Western-Blot-NBP2-15200-img0004.jpg)
![Western Blot: Bcl-2 Antibody (JF104-8) [NBP2-67182] - Lane 1: HL-60 cell lysate, Lane 2: THP-1 cell lysate, Lane 3: Jurkat cell lysateLysates/proteins at 10 ug/Lane. Predicted band size: 26 kDa Observed band size: 25 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-JF104-8-Western-Blot-NBP2-67182-img0010.jpg "Western Blot: Bcl-2 Antibody (JF104-8) [NBP2-67182] - Lane 1: HL-60 cell lysate, Lane 2: THP-1 cell lysate, Lane 3: Jurkat cell lysateLysates/proteins at 10 ug/Lane. Predicted band size: 26 kDa Observed band size: 25 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: Bcl-2 Antibody (JF104-8) [NBP2-67182] - Staining Bcl-2 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-JF104-8-Immunocytochemistry-Immunofluorescence-NBP2-67182-img0003.jpg)
![Immunohistochemistry-Paraffin: Bcl-2 Antibody (JF104-8) [NBP2-67182] - Analysis of paraffin-embedded mouse kidney tissue using anti-Bcl-2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-JF104-8-Immunohistochemistry-Paraffin-NBP2-67182-img0011.jpg)
![Flow Cytometry: Bcl-2 Antibody (JF104-8) [NBP2-67182] - Analysis of Jurkat cells with Bcl-2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-JF104-8-Flow-Cytometry-NBP2-67182-img0001.jpg)
![Western Blot: Bcl-2 Antibody (JF104-8) [NBP2-67182] - Western blot analysis of Bcl-2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: Jurkat cell lysate Lane 3: MCF-7 cell lysate](http://images.novusbio.com/fullsize/Bcl-2-Antibody-JF104-8-Western-Blot-NBP2-67182-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Bcl-2 Antibody (JF104-8) [NBP2-67182] - Staining Bcl-2 in A549 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-JF104-8-Immunocytochemistry-Immunofluorescence-NBP2-67182-img0002.jpg)
![Immunohistochemistry-Paraffin: Bcl-2 Antibody (JF104-8) [NBP2-67182] - Analysis of paraffin-embedded human colon cancer tissue using anti-Bcl-2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-JF104-8-Immunohistochemistry-Paraffin-NBP2-67182-img0004.jpg)
![Immunohistochemistry-Paraffin: Bcl-2 Antibody (JF104-8) [NBP2-67182] - Analysis of paraffin-embedded human lung cancer tissue using anti-Bcl-2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-JF104-8-Immunohistochemistry-Paraffin-NBP2-67182-img0005.jpg)
![Immunohistochemistry-Paraffin: Bcl-2 Antibody (JF104-8) [NBP2-67182] - Analysis of paraffin-embedded human tonsil tissue using anti-Bcl-2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-JF104-8-Immunohistochemistry-Paraffin-NBP2-67182-img0006.jpg)
![Immunohistochemistry-Paraffin: Bcl-2 Antibody (JF104-8) [NBP2-67182] - Analysis of paraffin-embedded mouse kidney tissue using anti-Bcl-2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-JF104-8-Immunohistochemistry-Paraffin-NBP2-67182-img0007.jpg)
![Western Blot: Bcl-2 Antibody (124) [NBP2-34443] - Western Blot Analysis of MCF-7 cells using Bcl-2 Antibody (124).](http://images.novusbio.com/fullsize/Bcl-2-Antibody-124-Western-Blot-NBP2-34443-img0005.jpg "Western Blot: Bcl-2 Antibody (124) [NBP2-34443] - Western Blot Analysis of MCF-7 cells using Bcl-2 Antibody (124).")
![Immunohistochemistry-Paraffin: Bcl-2 Antibody (124) [NBP2-34443] - Formalin-fixed, paraffin-embedded human tonsil stained with Bcl-2 Mouse Monoclonal Antibody (124).](http://images.novusbio.com/fullsize/Bcl-2-Antibody-124-Immunohistochemistry-Paraffin-NBP2-34443-img0006.jpg)
![Immunohistochemistry-Paraffin: Bcl-2 Antibody (124) [NBP2-34443] - Formalin-fixed, paraffin-embedded human non-Hodgkin's lymphoma stained with Bcl-2 MAb (124). Note nuclear membrane & cytoplasmic staining.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-124-Immunohistochemistry-Paraffin-NBP2-34443-img0001.jpg)
![Immunohistochemistry-Paraffin: Bcl-2 Antibody [NB100-56100] - FFPE section from a secondary follicle of the mouse lymph node stained for Bcl-2 expression using NB100-56100 at 1:2000. A, Abundant staining is seen in the mantle zone, and little staining is seen in the germinal center. (A1) is a higher magnification of (A).](http://images.novusbio.com/fullsize/Bcl-2-Antibody-Immunohistochemistry-Paraffin-NB100-56100-img0002.jpg "Immunohistochemistry-Paraffin: Bcl-2 Antibody [NB100-56100] - FFPE section from a secondary follicle of the mouse lymph node stained for Bcl-2 expression using NB100-56100 at 1:2000. A, Abundant staining is seen in the mantle zone, and little staining is seen in the germinal center. (A1) is a higher magnification of (A).")
![Western Blot: Bcl-2 Antibody (9D3) [NBP2-46567] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BCL-2.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-9D3-Western-Blot-NBP2-46567-img0012.jpg "Western Blot: Bcl-2 Antibody (9D3) [NBP2-46567] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BCL-2.")
![Immunohistochemistry: Bcl-2 Antibody (9D3) [NBP2-46567] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Bcl-2-Antibody-9D3-Immunohistochemistry-NBP2-46567-img0011.jpg)
![Immunohistochemistry: Bcl-2 Antibody (9D3) [NBP2-46567] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)](http://images.novusbio.com/fullsize/Bcl-2-Antibody-9D3-Immunohistochemistry-NBP2-46567-img0001.jpg)
![Immunohistochemistry: Bcl-2 Antibody (9D3) [NBP2-46567] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Bcl-2-Antibody-9D3-Immunohistochemistry-NBP2-46567-img0002.jpg)
![Immunohistochemistry: Bcl-2 Antibody (9D3) [NBP2-46567] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Bcl-2-Antibody-9D3-Immunohistochemistry-NBP2-46567-img0003.jpg)
![Immunohistochemistry: Bcl-2 Antibody (9D3) [NBP2-46567] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Bcl-2-Antibody-9D3-Immunohistochemistry-NBP2-46567-img0004.jpg)
![Immunohistochemistry: Bcl-2 Antibody (9D3) [NBP2-46567] - Analysis of Carcinoma of Human lung tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Bcl-2-Antibody-9D3-Immunohistochemistry-NBP2-46567-img0005.jpg)
![Immunohistochemistry: Bcl-2 Antibody (9D3) [NBP2-46567] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Bcl-2-Antibody-9D3-Immunohistochemistry-NBP2-46567-img0006.jpg)
![Immunohistochemistry: Bcl-2 Antibody (9D3) [NBP2-46567] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Bcl-2-Antibody-9D3-Immunohistochemistry-NBP2-46567-img0007.jpg)
![Immunohistochemistry: Bcl-2 Antibody (9D3) [NBP2-46567] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Bcl-2-Antibody-9D3-Immunohistochemistry-NBP2-46567-img0008.jpg)
![Immunohistochemistry: Bcl-2 Antibody (9D3) [NBP2-46567] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Bcl-2-Antibody-9D3-Immunohistochemistry-NBP2-46567-img0009.jpg)
![Immunohistochemistry: Bcl-2 Antibody (9D3) [NBP2-46567] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Bcl-2-Antibody-9D3-Immunohistochemistry-NBP2-46567-img0010.jpg)
![Western Blot: Bcl-2 Antibody (8C8) [NBP2-29630] - Analysis using the Azide and BSA Free version of NBP2-29630. Detection of Bcl-2 in 293T cell lsyate (30ug) using Bcl-2 HRP conjugated antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-8C8-Western-Blot-NBP2-29630-img0003.jpg "Western Blot: Bcl-2 Antibody (8C8) [NBP2-29630] - Analysis using the Azide and BSA Free version of NBP2-29630. Detection of Bcl-2 in 293T cell lsyate (30ug) using Bcl-2 HRP conjugated antibody. Image from verified customer review.")
![Immunohistochemistry-Paraffin: Bcl-2 Antibody (8C8) [NBP2-29630] - Immunohistochemistry-Paraffin: Bcl-2 Antibody (8C8) [NBP2-29630] - Formalin-fixed, paraffin-embedded human tonsil stained with Bcl-2 antibody (1:200), peroxidase-conjugate and DAB chromogen. Note cell membrane and cytoplasmic staining. Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 1mM EDTA, pH 8.0 for 10-20 min followed by cooling at RT for 20 min. Epitope unmasking in EDTA (compared to Citrate buffer) is superior.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-8C8-Immunohistochemistry-Paraffin-NBP2-29630-img0005.jpg)
![Western Blot: Bcl-2 Antibody (8C8) [NBP2-29630] - Analysis of Bcl-2 in human A) HeLa, B) A549 and C) MCF7 lysate using Bcl-2 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-8C8-Western-Blot-NBP2-29630-img0001.jpg)
![Western Blot: Bcl-2 Antibody [NBP2-30108] - Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines.Loading: 15 ug of lysates per lane.Antibodies: Bcl-2 NBP2-30108, (2 ug/mL), Bcl-2 from Company B, (2 ug/mL), beta-actin (1 ug/mL) and GAPDH (0.02 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-Western-Blot-NBP2-30108-img0011.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Bcl-2 Antibody [NBP2-30108] - Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines.Loading: 15 ug of lysates per lane.Antibodies: Bcl-2 NBP2-30108, (2 ug/mL), Bcl-2 from Company B, (2 ug/mL), beta-actin (1 ug/mL) and GAPDH (0.02 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/ Immunofluorescence: Bcl-2 Antibody - BSA Free [NBP2-30108] - Figure 7 Immunofluorescence Validation of Bcl-2 in Mouse Brain TissueImmunofluorescent analysis of 4% paraformaldehyde-fixed Mouse Brain Tissue labeling Bcl-2 with at 20 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green).](http://images.novusbio.com/fullsize/nbp2-30108_rabbit-polyclonal-bcl-2-antibody-271120242045654.gif)
![Immunohistochemistry-Paraffin: Bcl-2 Antibody [NBP2-30108] - Immunohistochemistry Validation of Bcl-2 in Human Kidney. Immunohistochemical analysis of paraffin-embedded Human Kidney using anti-Bcl-2 antibody (NBP2-30108) at 2ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-Immunohistochemistry-Paraffin-NBP2-30108-img0012.jpg)
![Western Blot: Bcl-2 Antibody [NBP2-30108] - Western Blot Validation in Human and Mouse Cell Lines. Loading: 15 ug of lysates per lane.Antibodies: Bcl-2 NBP2-30108, (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane A: Human Daudi cellsLane B: Mouse A-20 cells](http://images.novusbio.com/fullsize/Bcl-2-Antibody-Western-Blot-NBP2-30108-img0009.jpg)
![Western Blot: Bcl-2 Antibody [NBP2-30108] - Western Blot Validation in Human Cell Lines. Loading: 15 ug of lysates per lane.Antibodies: Bcl-2 NBP2-30108, (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-Western-Blot-NBP2-30108-img0010.jpg)
![Immunohistochemistry: Bcl-2 Antibody - BSA Free [NBP2-30108] - Figure 6 Immunohistochemistry Validation of Bcl-2 in Mouse Brain Tissue Immunohistochemical analysis of paraffin-embedded Mouse Brain Tissue using anti-Bcl-2 antibody at 5 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4;C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp2-30108_rabbit-polyclonal-bcl-2-antibody-271120241937773.gif)
![Immunocytochemistry/ Immunofluorescence: Bcl-2 Antibody - BSA Free [NBP2-30108] - Validation of Bcl-2 in Human Kidney Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed Human Kidney Cells labeling Bcl-2 with at 10 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).](http://images.novusbio.com/fullsize/nbp2-30108_rabbit-polyclonal-bcl-2-antibody-271120242045666.jpg)