ATPB Antibodies
We offer ATPB Antibodies for use in common research applications: ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, In vitro assay, Western Blot. Each ATPB Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our ATPB Antibodies can be used in a variety of model species: Canine, Human, Monkey, Mouse, Primate, Rat. Use the list below to choose the ATPB Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our ATPB polyclonal antibodies and browse our ATPB monoclonal antibody catalog.
Alternate Names for ATPB Antibodies
anti-ATPB antibody, anti-ATP5B antibody, anti-ATP synthase subunit beta, mitochondrial antibody, anti-ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide antibody, anti-ATPMB antibody, anti-ATPSBmitochondrial ATP synthase beta subunit antibody, anti-EC 3.6.3 antibody, anti-EC 3.6.3.14 antibody, anti-MGC5231 antibody, anti-mitochondrial ATP synthetase, beta subunit antibody
9 Results for "atpb" in Primary Antibodies
![Immunohistochemistry-Paraffin: ATPB Antibody [NBP1-90816] - Staining of human kidney, liver, prostate and small intestine using Anti-ATP5B antibody NBP1-90816 (A) shows similar protein distribution across tissues to independent antibody NBP1-90815 (B).](http://images.novusbio.com/fullsize/ATPB-Antibody-Immunohistochemistry-Paraffin-NBP1-90816-img0016.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: ATPB Antibody [NBP1-90816] - Staining of human kidney, liver, prostate and small intestine using Anti-ATP5B antibody NBP1-90816 (A) shows similar protein distribution across tissues to independent antibody NBP1-90815 (B).")
![<b>Independent Antibodies Validation and Genetic Strategies Validation.</b>Western Blot: ATPB Antibody [NBP1-90816] - Analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ATPB antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/ATPB-Antibody-Western-Blot-NBP1-90816-img0020.jpg)
![Immunocytochemistry/Immunofluorescence: ATPB Antibody [NBP1-90816] - Staining of human cell line U-2 OS shows localization to mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/ATPB-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90816-img0009.jpg)
![Immunohistochemistry-Paraffin: ATPB Antibody [NBP1-90816] - Staining of human small intestine shows moderate granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ATPB-Antibody-Immunohistochemistry-Paraffin-NBP1-90816-img0019.jpg)
![Western Blot: ATPB Antibody [NBP1-90816] - Western blot analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.](http://images.novusbio.com/fullsize/ATPB-Antibody-Western-Blot-NBP1-90816-img0010.jpg)
![Immunohistochemistry-Paraffin: ATPB Antibody [NBP1-90816] - Staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules and moderate immunoreactivity in glomeruli.](http://images.novusbio.com/fullsize/ATPB-Antibody-Immunohistochemistry-Paraffin-NBP1-90816-img0013.jpg)
![Immunohistochemistry-Paraffin: ATPB Antibody [NBP1-90816] - Staining of human prostate shows moderate granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ATPB-Antibody-Immunohistochemistry-Paraffin-NBP1-90816-img0014.jpg)
![Immunohistochemistry-Paraffin: ATPB Antibody [NBP1-90816] - Staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/ATPB-Antibody-Immunohistochemistry-Paraffin-NBP1-90816-img0018.jpg)
![Western Blot: ATPB Antibody (4E5) [NBP2-02249] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATPB (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATPB.](http://images.novusbio.com/fullsize/ATPB-Antibody-4E5-Western-Blot-NBP2-02249-img0019.jpg "Western Blot: ATPB Antibody (4E5) [NBP2-02249] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATPB (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATPB.")
![Immunocytochemistry/Immunofluorescence: ATPB Antibody (4E5) [NBP2-02249] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ATPB.](http://images.novusbio.com/fullsize/ATPB-Antibody-4E5-Immunocytochemistry-Immunofluorescence-NBP2-02249-img0018.jpg)
![Immunohistochemistry-Paraffin: ATPB Antibody (4E5) [NBP2-02249] - Staining of paraffin-embedded pancreas tissue using anti-ATPB mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ATPB-Antibody-4E5-Immunohistochemistry-Paraffin-NBP2-02249-img0013.jpg)
![Flow Cytometry: ATPB Antibody (4E5) [NBP2-02249] - Analysis of Jurkat cells, using anti-ATPB antibody, (Red) compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/ATPB-Antibody-4E5-Flow-Cytometry-NBP2-02249-img0017.jpg)
![Western Blot: ATPB Antibody (4E5) [NBP2-02249] Analysis of extracts (35ug) from 9 different cell lines by using anti-ATPB monoclonal antibody.](http://images.novusbio.com/fullsize/ATPB-Antibody-4E5-Western-Blot-NBP2-02249-img0015.jpg)
![Immunohistochemistry-Paraffin: ATPB Antibody (4E5) [NBP2-02249] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-ATPB mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ATPB-Antibody-4E5-Immunohistochemistry-Paraffin-NBP2-02249-img0004.jpg)
![Immunohistochemistry-Paraffin: ATPB Antibody (4E5) [NBP2-02249] - Staining of paraffin-embedded Adenocarcinoma of endometrium tissue using anti-ATPB mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ATPB-Antibody-4E5-Immunohistochemistry-Paraffin-NBP2-02249-img0005.jpg)
![Immunohistochemistry-Paraffin: ATPB Antibody (4E5) [NBP2-02249] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-ATPBmouse monoclonal antibody.](http://images.novusbio.com/fullsize/ATPB-Antibody-4E5-Immunohistochemistry-Paraffin-NBP2-02249-img0006.jpg)
![Immunohistochemistry-Paraffin: ATPB Antibody (4E5) [NBP2-02249] - Staining of paraffin-embedded breast tissue using anti-ATPB mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ATPB-Antibody-4E5-Immunohistochemistry-Paraffin-NBP2-02249-img0007.jpg)
![Immunohistochemistry-Paraffin: ATPB Antibody (4E5) [NBP2-02249] - Staining of paraffin-embedded Carcinoma of bladder tissue using anti-ATPB mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ATPB-Antibody-4E5-Immunohistochemistry-Paraffin-NBP2-02249-img0008.jpg)
![Immunohistochemistry-Paraffin: ATPB Antibody (4E5) [NBP2-02249] - Staining of paraffin-embedded endometrium tissue using anti-ATPB mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ATPB-Antibody-4E5-Immunohistochemistry-Paraffin-NBP2-02249-img0009.jpg)
![Immunohistochemistry-Paraffin: ATPB Antibody (4E5) [NBP2-02249] - Staining of paraffin-embedded liver tissue using anti-ATPBmouse monoclonal antibody.](http://images.novusbio.com/fullsize/ATPB-Antibody-4E5-Immunohistochemistry-Paraffin-NBP2-02249-img0010.jpg)
![Immunohistochemistry-Paraffin: ATPB Antibody (4E5) [NBP2-02249] - Staining of paraffin-embedded lung tissue using anti-ATPBmouse monoclonal antibody.](http://images.novusbio.com/fullsize/ATPB-Antibody-4E5-Immunohistochemistry-Paraffin-NBP2-02249-img0011.jpg)
![Immunohistochemistry-Paraffin: ATPB Antibody (4E5) [NBP2-02249] - Staining of paraffin-embedded Ovary tissue using anti-ATPB mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ATPB-Antibody-4E5-Immunohistochemistry-Paraffin-NBP2-02249-img0012.jpg)
![Flow Cytometry: ATPB Antibody (4E5) [NBP2-02249] - Analysis of Hela cells, using anti-ATPB antibody, (Red) compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/ATPB-Antibody-4E5-Flow-Cytometry-NBP2-02249-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: ATPB Antibody (4.3E8.D10) [NB600-1171] - Analysis of ATP Synthase beta (red) in HEK293T cells. Cells fixed with 4% formaldehyde were permeabilized and blocked with 1X PBS containing 5% BSA and 0.3% Triton X-100 for 1 hour at room temperature. Cells were probed with an ATP Synthase beta monoclonal antibody at a dilution of 1:100 overnight at 4C in 1X PBS containing 1% BSA and 0.3% Triton X-100, washed with 1X PBS, and incubated with a fluorophore-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:200 for 1 hour at room temperature. Nuclei (blue) were stained with DAPI.](http://images.novusbio.com/fullsize/ATPB-Antibody-4-3E8-D10-Immunocytochemistry-Immunofluorescence-NB600-1171-img0008.jpg "Immunocytochemistry/Immunofluorescence: ATPB Antibody (4.3E8.D10) [NB600-1171] - Analysis of ATP Synthase beta (red) in HEK293T cells. Cells fixed with 4% formaldehyde were permeabilized and blocked with 1X PBS containing 5% BSA and 0.3% Triton X-100 for 1 hour at room temperature. Cells were probed with an ATP Synthase beta monoclonal antibody at a dilution of 1:100 overnight at 4C in 1X PBS containing 1% BSA and 0.3% Triton X-100, washed with 1X PBS, and incubated with a fluorophore-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:200 for 1 hour at room temperature. Nuclei (blue) were stained with DAPI.")
![Immunocytochemistry/Immunofluorescence: ATPB Antibody (4.3E8.D10) [NB600-1171] - Indirect staining of rat neuronal/glial cell culture.](http://images.novusbio.com/fullsize/ATPB-Antibody-4-3E8-D10-Immunocytochemistry-Immunofluorescence-NB600-1171-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: ATPB Antibody (4.3E8.D10) [NB600-1171] - Analysis of ATP Synthase beta in HeLa cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a ATP Synthase beta monoclonal antibody at a dilution of 1:200 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. ATP Synthase beta staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.](http://images.novusbio.com/fullsize/ATPB-Antibody-4-3E8-D10-Immunocytochemistry-Immunofluorescence-NB600-1171-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: ATPB Antibody (4.3E8.D10) [NB600-1171] - Analysis of ATP Synthase beta in A431 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a ATP Synthase beta monoclonal antibody at a dilution of 1:200 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. ATP Synthase beta staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.](http://images.novusbio.com/fullsize/ATPB-Antibody-4-3E8-D10-Immunocytochemistry-Immunofluorescence-NB600-1171-img0007.jpg)
![Immunoprecipitation: ATPB Antibody (4.3E8.D10) [NB600-1171] - Analysis of rat neuronal/glial cell extract.](http://images.novusbio.com/fullsize/ATPB-Antibody-4-3E8-D10-Immunoprecipitation-NB600-1171-img0010.jpg)
![Immunoprecipitation: ATPB Antibody (4.3E8.D10) [NB600-1171] - Analysis of ATP Synthase beta was performed on THP-1 cells. Antigen-antibody complexes were formed by incubating 500ug of THP-1 whole cell lysate (in 500ul volume) with 5ul of an ATP Synthase beta monoclonal antibody overnight at 4C. The immune complexes were captured on 30ul of protein G agarose, washed extensively, and eluted with 6X Laemmli non-reducing sample buffer. Samples were resolved on an 8% SDS-PAGE gel, transferred to a PVDF membrane, and blocked with 5% milk in TBST for 1 hour at room temperature. The membrane was probed with an ATP Synthase beta monoclonal antibody at a dilution of 1:1000 overnight at 4C, washed in TBST, and probed with an HRP-conjugated goat anti-mouse IgG secondary at a dilution of 1:40,000 for 1 hour at room temperature. Chemiluminescent detection was performed using ECL substrate. Data courtesy of the Innovators Program.](http://images.novusbio.com/fullsize/ATPB-Antibody-4-3E8-D10-Immunoprecipitation-NB600-1171-img0009.jpg)
![Western Blot: ATPB Antibody [NBP1-90815] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/ATPB-Antibody-Western-Blot-NBP1-90815-img0015.jpg "Western Blot: ATPB Antibody [NBP1-90815] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.")
![Immunocytochemistry/Immunofluorescence: ATPB Antibody [NBP1-90815] - Staining of human cell line U-2 OS shows localization to mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/ATPB-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90815-img0007.jpg)
![Immunohistochemistry-Paraffin: ATPB Antibody [NBP1-90815] - Staining of human prostate shows strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ATPB-Antibody-Immunohistochemistry-Paraffin-NBP1-90815-img0020.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: ATPB Antibody [NBP1-90815] - Analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ATP5B antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/ATPB-Antibody-Western-Blot-NBP1-90815-img0009.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: ATPB Antibody [NBP1-90815] - Staining of human kidney, liver, prostate and small intestine using Anti-ATP5B antibody NBP1-90815 (A) shows similar protein distribution across tissues to independent antibody NBP1-90816 (B).](http://images.novusbio.com/fullsize/ATPB-Antibody-Immunohistochemistry-Paraffin-NBP1-90815-img0016.jpg)
![Immunohistochemistry-Paraffin: ATPB Antibody [NBP1-90815] - Staining of human small intestine shows moderate granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ATPB-Antibody-Immunohistochemistry-Paraffin-NBP1-90815-img0018.jpg)
![Immunohistochemistry-Paraffin: ATPB Antibody [NBP1-90815] - Staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/ATPB-Antibody-Immunohistochemistry-Paraffin-NBP1-90815-img0019.jpg)
![Immunohistochemistry-Paraffin: ATPB Antibody [NBP1-90815] - Staining of human liver shows strong granular cytoplasmic positivity in hepatocytes](http://images.novusbio.com/fullsize/ATPB-Antibody-Immunohistochemistry-Paraffin-NBP1-90815-img0021.jpg)
![Western Blot: ATPB Antibody [NBP1-54700] - ATP5B antibody - N-terminal region validated by WB using Proximal kidney tubules purfied from cortex at 1:1000.](http://images.novusbio.com/fullsize/ATPB-Antibody-Western-Blot-NBP1-54700-img0005.jpg "Western Blot: ATPB Antibody [NBP1-54700] - ATP5B antibody - N-terminal region validated by WB using Proximal kidney tubules purfied from cortex at 1:1000.")
![Immunohistochemistry-Paraffin: ATPB Antibody [NBP1-54700] - Human Muscle Tissue, Skeletal muscle cells (Indicated with Arrows) 4-8ug/ml.](http://images.novusbio.com/fullsize/ATPB-Antibody-Immunohistochemistry-Paraffin-NBP1-54700-img0006.jpg)
![Western Blot: ATPB Antibody [NBP1-54700] - ATP5B antibody - N-terminal region validated by WB using HepG2 cell lysate at 1.25ug/ml.](http://images.novusbio.com/fullsize/ATPB-Antibody-Western-Blot-NBP1-54700-img0004.jpg)
![Immunohistochemistry-Paraffin: ATPB Antibody [NBP1-54700] - Human Intestine Epithelial cells of intestinal villus (Indicated with Arrows).](http://images.novusbio.com/fullsize/ATPB-Antibody-Immunohistochemistry-Paraffin-NBP1-54700-img0001.jpg)
![Immunohistochemistry-Paraffin: ATPB Antibody (9A1O2) [NBP3-15354] - Immunohistochemistry of paraffin-embedded human esophageal cancer using ATPB Rabbit mAb (NBP3-15354) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/ATPB-Antibody-9A1O2-Immunohistochemistry-Paraffin-NBP3-15354-img0002.jpg "Immunohistochemistry-Paraffin: ATPB Antibody (9A1O2) [NBP3-15354] - Immunohistochemistry of paraffin-embedded human esophageal cancer using ATPB Rabbit mAb (NBP3-15354) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.")
![Immunohistochemistry-Paraffin: ATPB Antibody (9A1O2) [NBP3-15354] - Immunohistochemistry of paraffin-embedded rat kidney using ATPB Rabbit mAb (NBP3-15354) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/ATPB-Antibody-9A1O2-Immunohistochemistry-Paraffin-NBP3-15354-img0001.jpg)
![Western Blot: ATPB Antibody (9A1O2) [NBP3-15354] - analysis of extracts of various cell lines, using ATPB antibody at 1:20000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-15354_rabbit-atpb-mab-9a1o2-western-blot-510202312496..jpg)
![Immunocytochemistry/Immunofluorescence: ATPB Antibody (9A1O2) [NBP3-15354] - HepG2 cells using ATPB Rabbit mAb (dilution 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: 40x.](http://images.novusbio.com/fullsize/nbp3-15354_rabbit-atpb-mab-9a1o2-immunocytochemistry-immunofluorescence-5102023125019..jpg)
![Immunohistochemistry: ATPB Antibody (5F10) [NBP3-26490] - Image of ATPB Antibody (5F10) diluted at 1:100 and staining in paraffin-embedded human heart tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26490_rabbit-atpb-mab-5f10-262202415355720.jpg "Immunohistochemistry: ATPB Antibody (5F10) [NBP3-26490] - Image of ATPB Antibody (5F10) diluted at 1:100 and staining in paraffin-embedded human heart tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: ATPB Antibody (5F10) [NBP3-26490] - Image of ATPB Antibody (5F10) diluted at 1:100 and staining in paraffin-embedded human heart tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26490_rabbit-atpb-mab-5f10-27220248471912.jpg)
![Western Blot: ATPB Antibody (5F10) [NBP3-26490] - Positive Western Blot detected in: 293T whole cell lysate, HT29 whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate, Rat Heart tissue, Mouse Heart tissue. All lanes: ATPB Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 57 kDa Observed band size: 57 kDa](http://images.novusbio.com/fullsize/nbp3-26490_rabbit-atpb-mab-5f10-282202411363015.jpg)
![Immunohistochemistry: ATPB Antibody (5B3) [NBP3-43063] - Immunohistochemistry of paraffin-embedded rat heart using (ATPB antibody) at dilution of 1:500](http://images.novusbio.com/fullsize/nbp3-43063_mouse-atpb-mab-5b3-101220241723696.jpg "Immunohistochemistry: ATPB Antibody (5B3) [NBP3-43063] - Immunohistochemistry of paraffin-embedded rat heart using (ATPB antibody) at dilution of 1:500")
![Western Blot: ATPB Antibody (5B3) [NBP3-43063] - Hela cells were subjected to SDS PAGE followed by western blot with (ATPB antibody) at dilution of 1:10000](http://images.novusbio.com/fullsize/nbp3-43063_mouse-atpb-mab-5b3-101220241715419.jpg)