ASC/TMS1 Antibodies
ASC (apoptosis-associated speck-like protein containing a CARD), also known as TMS1 (target of methylation-induced silencing), is a known tumor suppressor and upon methylation ASC/TMS1 expression is silenced in many tumors. ASC/TMS1 has a theoretical weight of 22 kDa and functions in regulating apoptosis and immune response pathways. We offer antibodies to detect ASC/TMS1 that have been cited in 22+ publications with multiple 5-star reviews. Browse our extensive catalog for recombinant monoclonal, polyclonal, and ASC/TMS1-conjugated antibodies. Validated research applications for ASC/TMS1 antibodies include ELISA, Flow Cytometry, ICC, Immunofluorescence, IHC-Paraffin, Simple Western, and Knockout Validated Western Blot. Our ASC/TMS1 antibodies are suitable in multiple model species including Human, Mouse, and Rat. Choose the anti-ASC/TMS1 antibody best suited for your research needs by viewing the technical details, images, references, reviews, and related products. Each ASC/TMS1 antibody is fully covered by Novus’ Guarantee+.
Alternate Names for ASC/TMS1 Antibodies
anti-ASC/TMS1 antibody, anti-PYCARD antibody, anti-ASCMGC10332 antibody, anti-CARD5TMS antibody, anti-caspase recruitment domain protein 5 antibody, anti-Caspase recruitment domain-containing protein 5 antibody, anti-hASC antibody, anti-PYD and CARD domain containing antibody, anti-PYD and CARD domain-containing protein antibody, anti-Target of methylation-induced silencing 1 antibody, anti-TMS-1 antibody, anti-TMS1apoptosis-associated speck-like protein containing a CARD antibody
11 Results for "asc-tms1" in Primary Antibodies
![Simple Western: ASC/TMS1 Antibody [NBP1-78977] - Lane view shows a specific band for ASC TMS1 in 0.5 mg/ml of MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/ASC-TMS1-Antibody-Simple-Western-NBP1-78977-img0005.jpg "Simple Western: ASC/TMS1 Antibody [NBP1-78977] - Lane view shows a specific band for ASC TMS1 in 0.5 mg/ml of MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: ASC/TMS1 Antibody [NBP1-78977] - NLRP3 activation was evaluated by visualizing the co-localization of NLRP3 and apoptosis-associated speck-like protein (ASC) using immunocytochemistry (A). PLoS One. 2020 Jun 17;15(6):e0234039. doi: 10.1371/journal.pone.0234039. (Catalog # NBP1-78977AF647)](http://images.novusbio.com/fullsize/ASC-TMS1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-78977-img0008.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry: ASC/TMS1 Antibody [NBP1-78977] - Rats were infected by S. pneumoniae or given PBS sham control. 21.5 umoles/kg Mito-Vit-E (MVE) or vehicle was administered orally 30 minutes post-inoculation, and heart tissues were harvested 24 hours later. Heart sections were co-stained with anti-ASC (brown) and haematoxylin (blue). Negative control was stained with secondary antibody alone. Images are representative of a random selection of at least 3 sections of N = 6. PLoS One. 2015 Oct 8;10(10):e0139416. doi: 10.1371/journal.pone.0139416.](http://images.novusbio.com/fullsize/ASC-TMS1-Antibody-Immunohistochemistry-NBP1-78977-img0009.jpg)
![Western Blot: ASC/TMS1 Antibody [NBP1-78977] - Whole cell protein from (1) THP1 and (2) HL60 was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 4.0 ug/ml anti-ACS/TMS1 in 1% milk, and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/ASC-TMS1-Antibody-Western-Blot-NBP1-78977-img0007.jpg)
![Flow Cytometry: ASC/TMS1 Antibody - BSA Free [NBP1-78977] - An intracellular stain was performed on MCF7 cells with ASC/TMS1 NBP1-78977 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/ASC-TMS1-Antibody---BSA-Free-Flow-Cytometry-NBP1-78977-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: ASC/TMS1 Antibody [NBP1-78977] - Tested in MCF-7 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/ASC-TMS1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-78977-img0003.jpg)
![Immunohistochemistry: ASC/TMS1 Antibody [NBP1-78977] - Tested in mouse intestine at a 1:400 dilution.](http://images.novusbio.com/fullsize/ASC-TMS1-Antibody-Immunohistochemistry-NBP1-78977-img0004.jpg)
![Immunohistochemistry: ASC/TMS1 Antibody [NBP1-78978] - Analysis of ASC/TMS1 in mouse intestine using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/ASC-TMS1-Antibody-Immunohistochemistry-NBP1-78978-img0003.jpg "Immunohistochemistry: ASC/TMS1 Antibody [NBP1-78978] - Analysis of ASC/TMS1 in mouse intestine using DAB with hematoxylin counterstain.")
![Immunocytochemistry/Immunofluorescence: ASC/TMS1 Antibody [NBP1-78978] - Tested in A431 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/ASC-TMS1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-78978-img0002.jpg)
![Western Blot: ASC/TMS1 Antibody (OTI3E9) [NBP2-03818] - Analysis of extracts (35ug) from 9 different cell lines by usin g anti-ASC/TMS1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/ASC-TMS1-Antibody-3E9-Western-Blot-NBP2-03818-img0008.jpg "Western Blot: ASC/TMS1 Antibody (OTI3E9) [NBP2-03818] - Analysis of extracts (35ug) from 9 different cell lines by usin g anti-ASC/TMS1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).")
![Immunohistochemistry-Paraffin: ASC/TMS1 Antibody (OTI3E9) [NBP2-03818] - Staining of paraffin-embedded Human liver tissue using anti-ASC/TMS1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ASC-TMS1-Antibody-3E9-Immunohistochemistry-Paraffin-NBP2-03818-img0007.jpg)
![Flow Cytometry: ASC/TMS1 Antibody (OTI3E9) [NBP2-03818] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ASC/TMS1 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/ASC-TMS1-Antibody-3E9-Flow-Cytometry-NBP2-03818-img0001.jpg)
![Western Blot: ASC/TMS1 Antibody (OTI3E9) [NBP2-03818] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ASC/TMS1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ASC/TMS1.](http://images.novusbio.com/fullsize/ASC-TMS1-Antibody-3E9-Western-Blot-NBP2-03818-img0002.jpg)
![Immunohistochemistry-Paraffin: ASC/TMS1 Antibody (OTI3E9) [NBP2-03818] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-ASC/TMS1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ASC-TMS1-Antibody-3E9-Immunohistochemistry-Paraffin-NBP2-03818-img0003.jpg)
![Immunohistochemistry-Paraffin: ASC/TMS1 Antibody (OTI3E9) [NBP2-03818] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-ASC/TMS1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ASC-TMS1-Antibody-3E9-Immunohistochemistry-Paraffin-NBP2-03818-img0004.jpg)
![Immunohistochemistry-Paraffin: ASC/TMS1 Antibody (OTI3E9) [NBP2-03818] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-ASC/TMS1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ASC-TMS1-Antibody-3E9-Immunohistochemistry-Paraffin-NBP2-03818-img0005.jpg)
![Immunohistochemistry-Paraffin: ASC/TMS1 Antibody (OTI3E9) [NBP2-03818] - Staining of paraffin-embedded Human colon tissue using anti-ASC/TMS1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ASC-TMS1-Antibody-3E9-Immunohistochemistry-Paraffin-NBP2-03818-img0006.jpg)
![Western Blot: ASC/TMS1 Antibody (1C3D7) [NBP2-61682] - Analysis using PYCARD mAb against human PYCARD (AA: 1-120) recombinant protein. (Expected MW is 39.2 kDa)](http://images.novusbio.com/fullsize/ASC-TMS1-Antibody-1C3D7-Western-Blot-NBP2-61682-img0007.jpg "Western Blot: ASC/TMS1 Antibody (1C3D7) [NBP2-61682] - Analysis using PYCARD mAb against human PYCARD (AA: 1-120) recombinant protein. (Expected MW is 39.2 kDa)")
![Immunocytochemistry/Immunofluorescence: ASC/TMS1 Antibody (1C3D7) [NBP2-61682] - Analysis of Hela cells using PYCARD mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/ASC-TMS1-Antibody-1C3D7-Immunocytochemistry-Immunofluorescence-NBP2-61682-img0004.jpg)
![Immunohistochemistry-Paraffin: ASC/TMS1 Antibody (1C3D7) [NBP2-61682] - Analysis of stomach cancer tissues using PYCARD mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/ASC-TMS1-Antibody-1C3D7-Immunohistochemistry-NBP2-61682-img0006.jpg)
![Flow Cytometry: ASC/TMS1 Antibody (1C3D7) [NBP2-61682] - Analysis of HEK293 cells using PYCARD mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/ASC-TMS1-Antibody-1C3D7-Flow-Cytometry-NBP2-61682-img0002.jpg)
![Western Blot: ASC/TMS1 Antibody (1C3D7) [NBP2-61682] - Analysis using PYCARD mAb against HEK293 (1) and PYCARD (AA: 1-120)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/ASC-TMS1-Antibody-1C3D7-Western-Blot-NBP2-61682-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: ASC/TMS1 Antibody (1C3D7) [NBP2-61682] - Analysis of GC-7901 cells using PYCARD mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/ASC-TMS1-Antibody-1C3D7-Immunocytochemistry-Immunofluorescence-NBP2-61682-img0003.jpg)
![Immunohistochemistry-Paraffin: ASC/TMS1 Antibody (1C3D7) [NBP2-61682] - Analysis of ovarian cancer tissues using PYCARD mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/ASC-TMS1-Antibody-1C3D7-Immunohistochemistry-NBP2-61682-img0005.jpg)
![ELISA: ASC/TMS1 Antibody (1C3D7) [NBP2-61682] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/ASC-TMS1-Antibody-1C3D7-ELISA-NBP2-61682-img0001.jpg)
![Immunohistochemistry-Paraffin: ASC/TMS1 Antibody [NBP2-48925] - Analysis in human spleen and skeletal muscle tissues. Corresponding ASC/TMS1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ASC-TMS1-Antibody-Immunohistochemistry-Paraffin-NBP2-48925-img0013.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: ASC/TMS1 Antibody [NBP2-48925] - Analysis in human spleen and skeletal muscle tissues. Corresponding ASC/TMS1 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: ASC/TMS1 Antibody [NBP2-48925] - Staining of human liver, lung, skeletal muscle and spleen using Anti-ASC/TMS1 antibody NBP2-48925 (A) shows similar protein distribution across tissues to independent antibody NBP2-49133 (B).](http://images.novusbio.com/fullsize/ASC-TMS1-Antibody-Immunohistochemistry-Paraffin-NBP2-48925-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: ASC/TMS1 Antibody [NBP2-48925] - Immunofluorescent staining of human cell line MCF7 shows localization to nucleus, nucleoli & cytosol.](http://images.novusbio.com/fullsize/ASC-TMS1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-48925-img0005.jpg)
![Western Blot: ASC/TMS1 Antibody [NBP2-48925] - Analysis in human spleen tissue.](http://images.novusbio.com/fullsize/ASC-TMS1-Antibody-Western-Blot-NBP2-48925-img0017.jpg)
![Immunohistochemistry-Paraffin: ASC/TMS1 Antibody [NBP2-48925] - Immunohistochemical staining of human lymphoid tissues shows moderate cytoplasmic positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/ASC-TMS1-Antibody-Immunohistochemistry-NBP2-48925-img0019.jpg)
![Immunohistochemistry-Paraffin: ASC/TMS1 Antibody [NBP2-48925] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/ASC-TMS1-Antibody-Immunohistochemistry-Paraffin-NBP2-48925-img0006.jpg)
![Immunohistochemistry-Paraffin: ASC/TMS1 Antibody [NBP2-48925] - Staining of human liver shows moderate cytoplasmic positivity in Kupffer cells.](http://images.novusbio.com/fullsize/ASC-TMS1-Antibody-Immunohistochemistry-Paraffin-NBP2-48925-img0015.jpg)
![Immunohistochemistry-Paraffin: ASC/TMS1 Antibody [NBP2-48925] - Staining of human lung shows moderate cytoplasmic positivity in macrophages.](http://images.novusbio.com/fullsize/ASC-TMS1-Antibody-Immunohistochemistry-Paraffin-NBP2-48925-img0016.jpg)
![Immunohistochemistry-Paraffin: ASC/TMS1 Antibody [NBP2-48925] - Immunohistochemical staining of human spleen shows strong cytoplasmic positivity in cells in red pulp.](http://images.novusbio.com/fullsize/ASC-TMS1-Antibody-Immunohistochemistry-NBP2-48925-img0018.jpg)
![Western Blot: ASC/TMS1 Antibody (1C3D3) [NBP2-61676] - Analysis using PYCARD mAb against human PYCARD (AA: 1-120) recombinant protein. (Expected MW is 39.2 kDa)](http://images.novusbio.com/fullsize/ASC-TMS1-Antibody-1C3D3-Western-Blot-NBP2-61676-img0006.jpg "Western Blot: ASC/TMS1 Antibody (1C3D3) [NBP2-61676] - Analysis using PYCARD mAb against human PYCARD (AA: 1-120) recombinant protein. (Expected MW is 39.2 kDa)")
![Immunocytochemistry/Immunofluorescence: ASC/TMS1 Antibody (1C3D3) [NBP2-61676] - Analysis of Hela cells using PYCARD mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/ASC-TMS1-Antibody-1C3D3-Immunocytochemistry-Immunofluorescence-NBP2-61676-img0004.jpg)
![Immunohistochemistry-Paraffin: ASC/TMS1 Antibody (1C3D3) [NBP2-61676] - Analysis of stomach cancer tissues using PYCARD mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/ASC-TMS1-Antibody-1C3D3-Immunohistochemistry-NBP2-61676-img0005.jpg)
![Flow Cytometry: ASC/TMS1 Antibody (1C3D3) [NBP2-61676] - Analysis of HEK293 cells using PYCARD mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/ASC-TMS1-Antibody-1C3D3-Flow-Cytometry-NBP2-61676-img0002.jpg)
![Western Blot: ASC/TMS1 Antibody (1C3D3) [NBP2-61676] - Analysis using PYCARD mAb against HEK293 (1) and PYCARD (AA: 1-120)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/ASC-TMS1-Antibody-1C3D3-Western-Blot-NBP2-61676-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: ASC/TMS1 Antibody (1C3D3) [NBP2-61676] - Analysis of GC-7901 cells using PYCARD mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/ASC-TMS1-Antibody-1C3D3-Immunocytochemistry-Immunofluorescence-NBP2-61676-img0003.jpg)
![ELISA: ASC/TMS1 Antibody (1C3D3) [NBP2-61676] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/ASC-TMS1-Antibody-1C3D3-ELISA-NBP2-61676-img0001.jpg)
![Western Blot: ASC/TMS1 Antibody - BSA Free [NBP1-77297] - Western Blot Validation in Human THP-1 Cells. Loading: 15 ug of lysate per lane.Antibodies: ASC/TMS1 , (2 u/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp1-77297_rabbit-polyclonal-asc-tms1-antibody-2711202419553086.gif "Western Blot: ASC/TMS1 Antibody - BSA Free [NBP1-77297] - Western Blot Validation in Human THP-1 Cells. Loading: 15 ug of lysate per lane.Antibodies: ASC/TMS1 , (2 u/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
![<b>Independent Antibodies Validation. </b>Western Blot: ASC/TMS1 Antibody [NBP1-77297] - Independent Antibody Validation (IAV) via Protein Expression Profile in Cell LinesLoading: 15 ug of lysates per lane.Antibodies: ASC/TMS1 (NBP1-77297), (2 ug/mL), ASC, (2 ug/mL), beta-actin (1 ug/mL) and GAPDH (0.02 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit or goat anti-mouse IgG HRP conjugate.](http://images.novusbio.com/fullsize/ASC-TMS1-Antibody-Western-Blot-NBP1-77297-img0009.jpg)
![Immunohistochemistry: ASC/TMS1 Antibody [NBP1-77297] - Staining of human spleen tissue with antibody at 2.5 ug/ml.](http://images.novusbio.com/fullsize/ASC-TMS1-Antibody-Immunohistochemistry-NBP1-77297-img0006.jpg)
![Western Blot: ASC/TMS1 Antibody [NBP1-77297] - Western blot analysis of ASC in HL60 whole cell lysate in the absence (A) or presence (B) of blocking peptide with ASC antibody at 1 ug /ml.](http://images.novusbio.com/fullsize/ASC-TMS1-Antibody-Western-Blot-NBP1-77297-img0005.jpg)
![Immunohistochemistry: ASC/TMS1 Antibody - BSA Free [NBP1-77297] - Validation of ASC/TMS1 in Human Spleen Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed Human Spleen Tissue labeling ASC/TMS1 with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).](http://images.novusbio.com/fullsize/nbp1-77297_rabbit-polyclonal-asc-tms1-antibody-2711202419261825.gif)
![Immunocytochemistry/ Immunofluorescence: ASC/TMS1 Antibody - BSA Free [NBP1-77297] - Validation of ASC/TMS1 in HL60 Cells. Immunocytochemical analysis of HL60 cells using anti-ASC/TMS1 antibody at 5 ug/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-77297_rabbit-polyclonal-asc-tms1-antibody-271120241954153.gif)
![Immunohistochemistry-Paraffin: ASC/TMS1 Antibody [NBP2-49133] - Analysis in human spleen and skeletal muscle tissues. Corresponding PYCARD RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ASC-TMS1-Antibody-Immunohistochemistry-Paraffin-NBP2-49133-img0008.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: ASC/TMS1 Antibody [NBP2-49133] - Analysis in human spleen and skeletal muscle tissues. Corresponding PYCARD RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: ASC/TMS1 Antibody [NBP2-49133] - Staining of human liver, lung, skeletal muscle and spleen using Anti-ASC/TMS1 antibody NBP2-49133 (A) shows similar protein distribution across tissues to independent antibody NBP2-48925 (B).](http://images.novusbio.com/fullsize/ASC-TMS1-Antibody-Immunohistochemistry-Paraffin-NBP2-49133-img0009.jpg)
![Immunohistochemistry-Paraffin: ASC/TMS1 Antibody [NBP2-49133] - Staining of human liver shows moderate cytoplasmic positivity in Kupffer cells.](http://images.novusbio.com/fullsize/ASC-TMS1-Antibody-Immunohistochemistry-Paraffin-NBP2-49133-img0001.jpg)
![Immunohistochemistry-Paraffin: ASC/TMS1 Antibody [NBP2-49133] - Staining of human lung shows moderate cytoplasmic positivity in macrophages.](http://images.novusbio.com/fullsize/ASC-TMS1-Antibody-Immunohistochemistry-Paraffin-NBP2-49133-img0002.jpg)
![Immunohistochemistry-Paraffin: ASC/TMS1 Antibody [NBP2-49133] - Staining of human skeletal muscle shows low positivity in myocytes as expected.](http://images.novusbio.com/fullsize/ASC-TMS1-Antibody-Immunohistochemistry-Paraffin-NBP2-49133-img0003.jpg)
![Immunohistochemistry-Paraffin: ASC/TMS1 Antibody [NBP2-49133] - Staining of human spleen shows strong cytoplasmic positivity in cells in red pulp.](http://images.novusbio.com/fullsize/ASC-TMS1-Antibody-Immunohistochemistry-Paraffin-NBP2-49133-img0004.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: ASC/TMS1 Antibody [NBP2-49133] - Staining of human lymphoid tissues shows moderate cytoplasmic positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/ASC-TMS1-Antibody-Immunohistochemistry-Paraffin-NBP2-49133-img0005.jpg)
![Western Blot: ASC/TMS1 Antibody [NB100-870] - Staining of HeLa lysate (35 ug protein in RIPA buffer). Antibody at 0.2 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/ASC-TMS1-Antibody-Western-Blot-NB100-870-img0001.jpg "Western Blot: ASC/TMS1 Antibody [NB100-870] - Staining of HeLa lysate (35 ug protein in RIPA buffer). Antibody at 0.2 ug/mL. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: ASC/TMS1 Antibody [NB100-870] - Staining of paraffin embedded Human Liver. Antibody at 4 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/ASC-TMS1-Antibody-Immunohistochemistry-Paraffin-NB100-870-img0002.jpg)
![Western Blot: ASC/TMS1 [p Tyr144] Antibody [NBP3-23383] - Western blot analysis of mouse macrophage J774A.1 cells stimulated with pervanadate (1 mM for 30 min.), then untreated (-) or treated (+) with alkaline phosphatase. The blot was probed with rabbit polyclonal anti-ASC/TMS1 (Tyr-144) phospho-specific antibody (NBP3-23383) at 1:500.](http://images.novusbio.com/fullsize/nbp3-23383_rabbit-asc-tms1-p-tyr144-pab-19920231856914.jpg "Western Blot: ASC/TMS1 [p Tyr144] Antibody [NBP3-23383] - Western blot analysis of mouse macrophage J774A.1 cells stimulated with pervanadate (1 mM for 30 min.), then untreated (-) or treated (+) with alkaline phosphatase. The blot was probed with rabbit polyclonal anti-ASC/TMS1 (Tyr-144) phospho-specific antibody (NBP3-23383) at 1:500.")