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ASC/TMS1 Antibody [Unconjugated]

 (6 Publications)
 
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Western blot shows lysates of MCF-7 human breast cancer cell line and U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human ASC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3805) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for ASC at approximately 24 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.
Western blot shows lysates of MCF-7 human breast cancer cell line and U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human ASC Antigen Affinity-purified Polyclonal ...read more
Simple Western lane view shows lysates of MCF‑7 human breast cancer cell line and U937 human histiocytic lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for ASC at approximately 28 kDa (as ...read more
Genetic Strategies: Western blot shows lysates of MCF-7 human breast cancer parental cell line and ASC knockout MCF-7 cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human ASC Antigen ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, KO
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH
Validated by:
     

Genetic Strategies

    

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF3805-SP
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ASC/TMS1 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human ASC
Leu112-Ser195
Accession # Q9ULZ3
Specificity
Detects human ASC in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PYCARD
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Knockout Validated
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF3805 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ASC/TMS1 Antibody [Unconjugated]

  • apoptosis-associated speck-like protein containing a CARD
  • ASC
  • CARD5
  • caspase recruitment domain protein 5
  • Caspase recruitment domain-containing protein 5
  • hASC
  • PYCARD
  • PYD and CARD domain-containing protein
  • Target of methylation-induced silencing 1
  • TMS
  • TMS1
  • TMS-1

Background

Apoptosis-associated speck-like protein containing a CARD (ASC) is also known as Target of Methylation-mediated Silencing (TMS-1) and PYD and CARD-domain containing protein (PYCARD). ASC is a p53 target protein that serves as an adaptor to the pro-apoptotic Bax molecule. The interaction of ASC with Bax promotes apoptosis in response to many apoptotic stimuli.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ASC/TMS1 Antibody (AF3805)(6)

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 3 applications: ICC, IHC-P, Western Blot.


Filter By Application
ICC
(1)
IHC-P
(1)
Western Blot
(1)
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Human
(2)
Rat
(1)
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Showing Publications 1 - 6 of 6.
Publications using AF3805 Applications Species
A Gong, L Mi, F Wei, Y Zhuang, Y Song, C Pan, X Zhang, G Lin, Z Wu, Y Liu Downregulation of miR-137 Facilitates CD4+ T Cell Pyroptosis in Systemic Lupus Erythematosus via Stimulating AMPK Pathway Journal of Immunology Research, 2023-02-09;2023(0):1241774. 2023-02-09 [PMID: 36815949] (Western Blot, Human) Western Blot Human
SD Rao, Q Chen, Q Wang, EL Orth-He, M Saoi, AR Griswold, A Bhattachar, DP Ball, HC Huang, AJ Chui, DJ Covelli, S You, JR Cross, DA Bachovchin M24B aminopeptidase inhibitors selectively activate the CARD8 inflammasome Nature Chemical Biology, 2022-02-14;0(0):. 2022-02-14 [PMID: 35165443] (ICC, Human) ICC Human
Yana Liu, Shihong Zhang, Zhiwei Xue, Xiaoxia Zhou, Lin Tong, Jiachen Liao et al. Bone mesenchymal stem cells-derived miR-223-3p-containing exosomes ameliorate lipopolysaccharide-induced acute uterine injury via interacting with endothelial progenitor cells Bioengineered 2021-12-20 [PMID: 34738867]
Matteo Bertesi, Sebastian Fantini, Claudia Alecci, Roberta Lotti, Andrea Martello, Sandra Parenti et al. Promoter Methylation Leads to Decreased ZFP36 Expression and Deregulated NLRP3 Inflammasome Activation in Psoriatic Fibroblasts Frontiers in Medicine 2021-01-22 [PMID: 33585499]
Ball DP, Taabazuing CY, Griswold AR et al. Caspase-1 interdomain linker cleavage is required for pyroptosis Life Sci Alliance 3/1/2020 [PMID: 32051255]
CY Chang, HJ Shih, IT Huang, PS Tsai, KY Chen, CJ Huang Magnesium Sulfate Mitigates the Progression of Monocrotaline Pulmonary Hypertension in Rats Int J Mol Sci, 2019-09-18;20(18):. 2019-09-18 [PMID: 31540416] (IHC-P, Rat) IHC-P Rat

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for ASC/TMS1 Antibody (AF3805) (0)

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Secondary Antibodies

 

Isotype Controls

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Blogs on ASC/TMS1.

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The NLRP3 Inflammasome: Macrophage Activator & Pathology Driver
By Victoria Osinski, PhDWhat is the NLRP3 Inflammasome?With its critical role in innate immunity, the nucleotide-binding oligomerization domain-like receptor family, pyrin domain containing 3 (NLRP3) inflammasome ...  Read full blog post.
Pyroptosis: Mechanisms mediating cell death and pro-inflammatory cytokine release
By Victoria OsinskiPyroptosis is an inflammatory form of programmed cell death characterized by the release of pro-inflammatory cytokines IL-1 beta and IL-18.1,10 It is a process distinct from apoptosis and necrosis (T...  Read full blog post.
The inflammasome: an inflammation-initiating machine, Novus Biologicals
By Stephanie Melchor The inflammasome is a large, multimeric protein complex found primarily in innate immune cells, which are white blood cells that can attack a wide range of pathogenic threats. Three main elements ...  Read full blog post.
Immunity’s flipside: Microglia promote Alzheimer’s pathology during inflammation
By Jamshed Arslan Pharm.D. Microglia are brain's macrophages. In Alzheimer's disease (AD), microglia clear up protein aggregates called amyloid beta plaques. The connection between immune activation and AD is unclea...  Read full blog post.
CARD & NFKB Antibodies for Apoptosis Research
Apoptosis is one of the main types of programmed cell death which involves a cascade of biochemical events leading to specific cell morphology characteristics and ultimately death of cells.Caspases play crucial roles in modulating cellular signaling...  Read full blog post.
Read our latest blog and use the new citation tool on bio-techne.com

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Bioinformatics

Gene Symbol PYCARD
Uniprot