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PMS1 Antibody (PCRP-PMS1-2E11)

Images

 
Immunocytochemistry/ Immunofluorescence: PMS1 Antibody (PCRP-PMS1-2E11) [NBP3-13739] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using PMS1 Antibody (PCRP-PMS1-2E11) [CF488]. PMS1 localized to ...read more
Flow Cytometry: PMS1 Antibody (PCRP-PMS1-2E11) [NBP3-13739] - Flow cytometric analysis of PFA-fixed HeLa cells. PMS1 antibody (PCRP-PMS1-2E11) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).
Protein Array: PMS1 Antibody (PCRP-PMS1-2E11) [NBP3-13739] - Analysis of Protein Array containing more than 19,000 full-length human proteins using PMS1 Antibody (PCRP-PMS1-2E11).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA, Flow, ICC/IF, IP, MA
Clone
PCRP-PMS1-2E11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

PMS1 Antibody (PCRP-PMS1-2E11) Summary

Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14142)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non-hazardous.
Immunogen
Recombinant full-length human PMS1 protein (Uniprot: P54277)
Localization
Nucleus, Nuclear bodies
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PMS1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunoprecipitation 1-2 ug/100-500 ug protein
  • Protein Array
Application Notes
ELISA: concentration is assay dependent

Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
Theoretical MW
105.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for PMS1 Antibody (PCRP-PMS1-2E11)

  • DKFZp781M0253
  • FLJ98259
  • HNPCC3
  • hPMS1
  • human homolog of yeast mutL
  • mismatch repair gene PMSL1
  • PMS1 postmeiotic segregation increased 1 (S. cerevisiae)
  • PMS1 protein homolog 1
  • PMSL1DNA mismatch repair protein PMS1
  • postmeiotic segregation increased (S. cerevisiae) 1
  • rhabdomyosarcoma antigen MU-RMS-40.10B
  • rhabdomyosarcoma antigen MU-RMS-40.10E

Background

PMS1 was identified by its homology to a yeast protein involved in DNA mismatch repair. A role for this protein in mismatch repair has not been proven. However, the protein forms heterodimers with MLH1, a DNA mismatch repair protein, and some cases of hereditary nonpolyposis colorectal cancer have been found to have mutations in the gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

FAQs for PMS1 Antibody (NBP3-13739) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PMS1
Uniprot