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Glutamate Homeostasis Pathway Bioinformatics

Disease and disorder research has been conducted in relation to the Glutamate Homeostasis Pathway and Nervousness, Sclerosis, Multiple Sclerosis, Neurotoxicity Syndromes, Pain. The study of the Glutamate Homeostasis Pathway has been mentioned in research publications which can be found using our bioinformatics tool below. The Glutamate Homeostasis Pathway has been researched in relation to Transport, Cell Death, Pathogenesis, Synaptic Transmission, Localization. The Glutamate Homeostasis Pathway complements our catalog of research reagents including antibodies and ELISA kits against GLUTAMATE TRANSPORTER, GLIAL GLUTAMATE TRANSPORTER, GLUTAMATE/ASPARTATE TRANSPORTER, APC, AQP4.

Top Research Reagents

We have 2101 products for the study of the Glutamate Homeostasis Pathway that can be applied to Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot from our catalog of antibodies and ELISA kits.

NBP1-20136
Western Blot: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:1000 incubated overnight at 4C.Immunocytochemistry/Immunofluorescence: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Human NPCs differentiate into mature neurons. Immunofluorescence staining of differentiated neurons derived from human dorsal NPCs (1323-2 line, day 35 after differentiation) for mature cortical neuronal markers expressed in the nucleus (BRN2, TBR1, NeuN) and cytoplasm (MAP2), glial markers (SOX9, GFAP, GLT1), and dorsal forebrain marker (FOXG1). Nuclei stained with DAPI, shown as an overlay over brightfield images. The merge is an overlay of the neuronal and glial markers. Scale bar 100 um. DAPI 4,6'-diamino-2-phenylindole Image collected and cropped by CiteAb from the following publication (//stemcellres.biomedcentral.com/articles/10.1186/s13287-018-0812-6) licensed under a CC-BY license.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, Flow, ICC/IF

12 Publications
NB100-1869
Flow (Intracellular): EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - Intracellular staining of HEK293 cells (1 x 10^6 cells/mL) with SLC1A3 antibody (orange) stained at a dilution of 1:500. Detected with a GtxRb Dylight 488 secondary. Shown with the secondary control (blue).Immunohistochemistry: EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - Staining of SLC1A3 in rat cerebellum sections

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ELISA, Flow

     1 Review

33 Publications
NBP1-87679
Immunohistochemistry-Paraffin: Aquaporin-4 Antibody [NBP1-87679] - Analysis in human cerebral cortex and liver tissues.   Corresponding AQP4 RNA-seq data are presented for the same tissues.Western Blot: Aquaporin-4 Antibody [NBP1-87679] - Analysis in human brain tissue.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, IHC, IHC-Fr

     2 Reviews

21 Publications
NBP1-88191
Immunohistochemistry-Paraffin: Carbonic Anhydrase I/CA1 Antibody [NBP1-88191] - Analysis in human rectum and pancreas tissues. Corresponding CA1 RNA-seq data are presented for the same tissues.Western Blot: Carbonic Anhydrase I/CA1 Antibody [NBP1-88191] - Comparative proteome analysis of 3xTg-AD and control samples at different stages of AD. Immunoblot analysis of most regulated hits. Soluble fractions of brain proteins were analyzed from four 2-month-old control and 3xTg-AD mice animals, respectively. Hebp1 and Glo1 levels were consistently elevated in the transgenic animals as compared to wild type controls. Ca1 levels were reduced in transgenic animals. Image collected and cropped by CiteAb from the following publication (https://elifesciences.org/articles/47498), licensed under a CC-BY license.

Rabbit Polyclonal
Species Human, Mouse
Applications WB, IHC, IHC-P

3 Publications
NBP1-89766
Immunohistochemistry-Paraffin: Glutaminase Antibody [NBP1-89766] - Staining in human kidney and skeletal muscle tissues using NBP1-89766 antibody. Corresponding GLS RNA-seq data are presented for the same tissues.Western Blot: Glutaminase Antibody [NBP1-89766] - Region-based expression of the pyruvate recycling pathway marker protein glutaminase (GLT) the VMN of icv Lz-pretreated male and female rats. GLT protein was measured by Western blot in the caudal  (c; F(7,40) = 11.67; p < 0.0001) VMN of V/V, V/INS, Lz/V, and Lz/INS groups of male (M; left-hand side) and female (F; right-hand side) rats. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. For each VMN segment, data depict mean normalized GAD O.D. values +/- S.E.M. VMN = Ventromedial hypothalamic nucleus; V = Vehicle; L = letrozole; INS = insulin. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33238883/) licensed under a CC-BY license.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

5 Publications
NBP2-16679
Western Blot: Glutamate Dehydrogenase Antibody [NBP2-16679] - Metabolic alterations in the MPC1-/- cells expressed significantly higher levels glutaminolysis related proteins GDH (Glutamate Dehydrogenase) and GLS (NBP2-29940), but there was no significant change in the PDHE1 protein expression in these cells as shown in representative western blots. The alpha-Tublin was used as loading control. ***P<0.001. Data were expressed as mean +/- SEM. Three replicated experiments were carried out with the similar results. Image collected and cropped by CiteAb from the following publication (www.oncotarget.com/lookup/doi/10.18632/oncotarget.18199) licensed under a CC-BY license.Immunocytochemistry/Immunofluorescence: Glutamate Dehydrogenase Antibody [NBP2-16679] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Glutamate Dehydrogenase protein stained by Glutamate Dehydrogenase antibody  diluted at 1:500. Blue: Hoechst 33342 staining.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ELISA, ICC/IF

4 Publications
236-EG
Recombinant Human EGF Protein (Catalog # 236-EG) has a molecular weight (MW) of 6.7 kDa as analyzed by SEC-MALS, suggesting that this protein is a monomer.Recombinant Human EGF (Catalog # 236‑EG) stimulates cell proliferation of the Balb/3T3 mouse embryonic fibroblast cell line. The ED<sub>50</sub> for this effect is 20‑100 pg/mL.


Species Human
Applications BA

847 Publications
7954-GM/CF
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED<sub>50</sub> for this effect is 6-30 pg/mL.


Species Human
Applications BA

3 Publications
NBP2-42388
Immunohistochemistry-Paraffin: LAMC2 Antibody (CL2980) [NBP2-42388] - Staining in human fallopian tube and liver tissues. Corresponding LAMC2 RNA-seq data are presented for the same tissues.Western Blot: LAMC2 Antibody (CL2980) [NBP2-42388] - Analysis in A-431 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-LAMC2 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Mouse Monoclonal
Species Human
Applications WB, ICC/IF, IHC

4 Publications
NB300-318
Dual RNAscope ISH-IHC: xCT Antibody [NB300-318] - Formalin-fixed paraffin-embedded tissue sections of human stomach were probed for xCT mRNA (ACD RNAScope Probe, catalog #422688; Fast Red chromogen, ACD catalog # 322750). Adjacent tissue section was processed for immunohistochemistry using Rabbit Polyclonal  (Novus Biologicals catalog # NB300-318) at 0.25ug/mL with 1 hour incubation at room temperature followed by incubation with anti-rabbit IgG VisUCyte HRP Polymer Antibody (Catalog # VC003) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to glandular cells.Simple Western: xCT Antibody [NB300-318] - Simple Western lane view shows a specific band for xCT using NB300-318 at 25 ug/ml in HeLa and HeLa + DEM cell lysates. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications IP (-), WB, Simple Western

     3 Reviews

79 Publications
NB300-141
Immunohistochemistry: GFAP Antibody [NB300-141] - Analysis of a rat cerebellum section stained with rabbit polyclonal antibody to GFAP, NB300-141, dilution 1:5000 in green and mouse monoclonal antibody to MeCP2, dilution 1:500, in red. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 1 hour, cut to 45 uM, and free-floating sections were stained with above antibodies. The GFAP antibody stains the network of astrocytic cells and the processes of Bergmann glia in the molecular layer. The MeCP2 antibody specifically labels nuclei of certain neurons.Immunocytochemistry/Immunofluorescence: GFAP Antibody [NB300-141] - Rat neurons stained with Neurofilament Heavy antibody NB300-217 (red) and GFAP antibody NB300-141 (green).

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, ICC/IF

     12 Reviews

108 Publications
NBP2-59319
Western Blot analysis of Rat brain membrane lysate showing detection of EAAT3 protein using Mouse Anti-EAAT3 Monoclonal Antibody, Clone S180-41 (NBP2-59319). Primary Antibody: Mouse Anti-EAAT3 Monoclonal Antibody (NBP2-59319) at 1:1000.Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-EAAC1 Monoclonal Antibody, Clone S180-41 (NBP2-59319). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-EAAC1 Monoclonal Antibody (NBP2-59319) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) EAAC1 Antibody (D) Composite.

Mouse Monoclonal
Species Human, Rat
Applications WB, ICC/IF, IP

NB110-41404
Western Blot: Glutamine Synthetase Antibody [NB110-41404] - Analysis of glutamine synthase. 40ug of lysates from mouse (Lanes M), rat (Lane R), pig (Lane P), bovine (Lane B), or human (Lane Hu) retina were probed. A 42 kDa band was identified in lysates from retinas of all species.Immunocytochemistry/Immunofluorescence: Glutamine Synthetase Antibody [NB110-41404] - Immunofluorescence using NB110-41404. Submitted via verified customer review.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

     1 Review

11 Publications
NBP2-67416
Western Blot: Integrin beta 3/CD61 Antibody (SJ19-09) [NBP2-67416] - Analysis of Integrin beta 3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HUVEC cell lysateLane 2: mouse marrow tissue lysateImmunocytochemistry/Immunofluorescence: Integrin beta 3/CD61 Antibody (SJ19-09) [NBP2-67416] - Staining Integrin beta 3 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X-100/PBS.

Rabbit Monoclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

     2 Reviews

6 Publications
NB100-91662
Western Blot: APC Antibody [NB100-91662] - Lane1:Hela cell lysate.  Lane2:HEK293T cell lysate.  Lane3:Rat testis tissue lysate.  Immunocytochemistry/Immunofluorescence: APC Antibody [NB100-91662] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-APC Antibody at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, Flow, ICC/IF

2 Publications
NBP2-81802
Western Blot: SLC1A7 Antibody [NBP2-81802] - Analysis of SLC1A7 in C2C12 cell lysate with SLC1A7 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.Immunocytochemistry/ Immunofluorescence: SLC1A7 Antibody - BSA Free [NBP2-81802] - Immunofluorescence of SLC1A7 in mouse kidney tissue with SLC1A7 antibody at 20 u/ml.

Rabbit Polyclonal
Species Human, Mouse
Applications WB, ELISA, ICC/IF

NBP3-13360
Immunocytochemistry/Immunofluorescence: EAAT4 Antibody [NBP3-13360] - EAAT4 antibody detects EAAT4 protein by immunofluorescent analysis. Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EAAT4 stained by EAAT4 antibody (NBP3-13360) diluted at 1:500. Red: alpha Tubulin, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:500. Blue: Fluoroshield with DAPI.Immunohistochemistry-Paraffin: EAAT4 Antibody [NBP3-13360] - EAAT4 antibody detects EAAT4 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. EAAT4 stained by EAAT4 antibody (NBP3-13360) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Rabbit Polyclonal
Species Mouse, Rat
Applications ICC/IF, IHC, IHC-Fr