Hu,
Mu,
Rt,
Ch,
Dr,
I,
MaSpecies Glossary
Reactivity: Hu, Mu, Rt, Ch, Dr, I, Ma
WB, Simple Western, ICC/IF, IHC, Dual ISH-IHC, IHC, KO
Applications: WB, Simple Western, ICC/IF, IHC, Dual ISH-IHC, IHC, KO
Host: Rabbit Polyclonal
Sample Size Available
Sizes: Sample Size Available
![Immunohistochemistry: Tyrosine Hydroxylase Antibody [NB300-109] - D409H GBA1 expression promotes reduction of dopaminergic fiber densities & motor deficit in the striatum of hA53T alpha -synuclein transgenic mice. a, The striatal TH-immunopositive fiber density was assessed in the striatum of the indicated genotypes at 6 months of age (n = 6 per each group). The scale bar is 250 μm. b, The optical densities of TH positive signals were quantified. c & d, Rotarod & Pole test were assessed at 6 months of age with the indicated genotypes (n = 7 per each group). e, the body weight of mice were measured with the indicated genotypes (n = 15 per each group). DATA are expressed as mean ± SEM Image collected & cropped by CiteAb from the following publication (//actaneurocomms.biomedcentral.com/articles/10.1186/s40478-018-0538-9), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-109_rabbit-polyclonal-tyrosine-hydroxylase-antibody-310202415371995.jpg)
![Western Blot: Tyrosine Hydroxylase Antibody [NB300-109] - Dysregulation of RNF146-PARP1 pathways in PD pathogenesis. (C) Expression of PARsylated proteins, RNF146, TH & phosphorylated alpha -synuclein (pS129-a-syn) in postmortem striatum from PD patients & age-matched control subjects (Ctrl) determined by WBs using designated antibodies. Image collected & cropped by CiteAb from following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.21828), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-109_rabbit-polyclonal-tyrosine-hydroxylase-antibody-310202415293344.jpg)
![Immunocytochemistry/ Immunofluorescence: Tyrosine Hydroxylase Antibody [NB300-109] - Image showing differentiation of neurospheres as evidenced morphologically (A) & through staining by Tyrosine Hydroxylase (B) to show the ability to derive dopaminergic neurons from the rat progenitor cells. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30042680), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-109_rabbit-polyclonal-tyrosine-hydroxylase-antibody-310202415392574.jpg)
![Western Blot: Tyrosine Hydroxylase Antibody [NB300-109] - Immunoblots of tyrosine hydroxylase in the cricket & cockroach brain.Protein extracts (8 μl & 16 μl) from the male cricket, Gryllus bimaculatus & male cockroach, Periplaneta americana were electrophoresed, transferred to a membrane & reacted with anti-tyrosine hydroxylase (TH). The anti-TH recognized a single band of protein of ~ 66 kDa (arrow heads) in both the cricket (left two lanes) & cockroach (right two lanes). M, molecular marker. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0160531), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-109_rabbit-polyclonal-tyrosine-hydroxylase-antibody-31020241535660.jpg)
![Immunohistochemistry: Tyrosine Hydroxylase Antibody [NB300-109] - D409H GBA1 expression shortens lifespan & leads to dopaminergic degeneration in the hA53T alpha -synuclein transgenic mice. a, Survival was monitored from littermates with the following genotypes: hA53Ta-Syn/GBA1+/+ (n = 15), hA53T alpha -Syn/GBA1+/D409H(n = 16), hA53T alpha -Syn/GBA1D409H/D409H (n = 18) mice. GBA1 mutation induces the lethal phenotype & TH-positive neuronal loss in the SNpc of A53T mutant alpha -synuclein transgenic mice. b & c, The number of TH-positive neurons in the SNpc was counted using stereological analysis with the indicated genotypes at 6 months of age. The scale bar is 200 μm. DATA are expressed as mean ± SEM (n = 6 for the each group) Image collected & cropped by CiteAb from the following publication (//actaneurocomms.biomedcentral.com/articles/10.1186/s40478-018-0538-9), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-109_rabbit-polyclonal-tyrosine-hydroxylase-antibody-310202415395928.jpg)
![Immunocytochemistry/ Immunofluorescence: Tyrosine Hydroxylase Antibody [NB300-109] - Tyrosine hydroxylase immunoreactivity in the deutocerebrum & medial antennal lobe tract.Tyrosine hydroxylase (TH) immunoreactivity is shown in magenta (A, D, G, J) & synapsin immunoreactivity in green (B, E, H, K). Corresponding merged images (C, F, I, L). A-C: Frontal sections through the antennal lobe (AL). Insets indicate single optical slice ( 0.05. b Heatmaps of proteins significantly enriched under toxic stress (10 µM CCCP; 20 nM rotenone) & their validation by western blot (Input, total levels & S-HSP90 bait isolate, chaperome network associated). Mean ± SEM, n = 3 individual values from the different experiments shown as points, t-test, ***p < 0.001; *p < 0.05. c Western blot & quantification of rotenone stress increase in the p-TH:TH ratio & its reduction by PU-H71 (200 nM). p-ERK/ERK, cell viability control; beta -actin, protein loading control. Mean ± SEM, n = 3 individual values from the different experiments shown as points, One-way ANOVA with Tukey’s post-hoc, ***p < 0.001; **p < 0.01. d Total intracellular dopamine levels in PD mDA neurons in conditions of toxic stress & under PU-H71 rescue; Mean ± SEM, n = 4–5 individual values from the different experiments shown as points, t-test, *p < 0.05. e PU-H71 treatment of rotenone- or CCCP-stressed mDA neurons significantly increases their viability, as measured by total ATP levels; means ± SEM, n = 3–8 from independent differentiations, t-test, ***p < 0.001; **p < 0.01; *p < 0.05 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30341316), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-109_rabbit-polyclonal-tyrosine-hydroxylase-antibody-31020241620377.jpg)
![Immunocytochemistry/ Immunofluorescence: Tyrosine Hydroxylase Antibody [NB300-109] - ErbB4 protein expression in mesencephalic nuclei.The overview on the mesencephalon (B) shows dense ErbB4 expression (mAb-10, green channel) in the dopaminergic nuclei SNc & VTA that is clearly above background levels, whereas expression in the red nucleus is weaker. Higher magnification shows abundant immunoreactivity on neuropil (C–E, C'–E'). At 100x magnification, a dense network of ErbB4-positive neuropil surrounding a neuron (asterisk) is evident in the VTA (E”). The red channel shows neurofilament H-immunofluorescence in panels (B–E) & TH-immunofluorescence in (F–G). Co-immunolabeling reveals that subsets of TH-positive dopaminergic somata express ErbB4 in the VTA (F) & SNc (G); coexpressing neurons are indicated by arrowheads (F–G), whereas lack of ErbB4 signal is indicated by arrows (G). The location of the overview image within the monkey brain is indicated by a red rectangle in (A). The location of the profiles through mesencephalic nuclei is indicated by yellow boxes in (A) & by rectangles in (B). Images were obtained with a confocal microscope. Scale bar = 3 mm (B), 400 µm (C–E), 175 µm (C'–E', F–G), 70 µm (E”). Abbreviations: cerebral peduncle (cp), oculomotor nerve (3n), substantia nigra pars reticulata (SNr). Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0027337), licensed under a CC0-1.0 license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-109_rabbit-polyclonal-tyrosine-hydroxylase-antibody-310202416212334.jpg)
![Immunocytochemistry/ Immunofluorescence: Tyrosine Hydroxylase Antibody [NB300-109] - Tyrosine hydroxylase immunoreactivity in mushroom body.All panels show a right brain hemisphere, except panels M-O (posterior to top). Tyrosine hydroxylase (TH) immunoreactivity shown in magenta (A, D, G, J, M) & synapsin immunoreactivity (green: B, E, H, K, N). Corresponding merged images in C, F, I, L, O. A-C: Frontal sections through lateral protocerebrum. 2 groups of TH-positive cell bodies (DCa1 & 2) labeled. DCa2 appears to send axons ventrally (arrowhead). Superior lateral protocerebrum (SLP) as well as medial & lateral calyces (MCA & LCA) innervated by TH-positive fibers. D-F: Frontal sections through vertical lobe (VL) & parts of medial calyx (MCA), which receive many fine TH-positive fibers. G-I: Horizontal sections through a tip of vertical lobe & dorsal parts of calyces. Both calyces & vertical lobe innervated by fibrous TH-positive fibers. Note TH-immunoreactive fibers exclusively innervate synapsin-positive neuropil layers (NLs) of calyx where dendrites of Kenyon cells receive synaptic inputs from antennal-lobe projection neurons. J-L: Horizontal sections through calyces, ventral to panels G-I. Here, 3clusters of TH-positive cell bodies (DCa1, DCa2, & DSP1) visible. Immunoreactive fibers mainly invade anterior layers including gamma layer ( gamma ) in vertical lobe. M-O: Horizontal sections through distal portions of medial lobes (MLs), invaded by fine TH-positive fibers exhibiting moderate immunolabeling (arrows). in inferior medial protocerebrum (IMP) anterior to medial lobe, a pair of DIP1 clusters (triangles) is situated. D, dorsal; KFL, Kenyon fiber layer; LCA, lateral calyx; LO, lobula; M, medial; P, posterior; PED, pedunculus; SMP, superior medial protocerebrum. Scale bars = 100 μm. Image collected & cropped by CiteAb from following publication (//dx.plos.org/10.1371/journal.pone.0160531), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-109_rabbit-polyclonal-tyrosine-hydroxylase-antibody-310202416212326.jpg)
![Immunohistochemistry: Tyrosine Hydroxylase Antibody [NB300-109] - Characterization of the nigrostriatal pathway of LRRK2 conditional transgenic mice. A, Representative TH immunohistochemistry of the midbrain coronal sections of 22-month-old LRRK2 GS & GSDA transgenic & age-matched littermate controls. B, C, Stereological assessment of TH-positive (B) & Nissl-positive (C) neurons in the SNpc (control, n = 9; GS, n = 9; GS/DA, n = 9). Data are the mean number of cells per region ± SEM, n = 9 mice per group. Statistical significance was determined by two-tailed unpaired Student’s t test. D, Representative images of TH immunostaining of nerve terminals in the striatum of LRRK2 conditional transgenic mice at 22 months of age. E, Quantitation of TH immunostaining in the striatum using ImageJ software (NIH; control, n = 7; GS, n = 7; GS/DA, n = 7). Differences between groups were assessed by two-way ANOVA. Bars represent the mean ± SEM (n ≥ 5 animals/genotype). n.s., Nonsignificant. Image collected & cropped by CiteAb from the following publication (//www.eneuro.org/lookup/doi/10.1523/ENEURO.0004-17.2017), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-109_rabbit-polyclonal-tyrosine-hydroxylase-antibody-310202416205143.jpg)
![Immunocytochemistry/ Immunofluorescence: Tyrosine Hydroxylase Antibody [NB300-109] - Tyrosine hydroxylase (TH) immunoreactivity in mushroom body. Panels show a right brain hemisphere, except panels M-O (posterior top). Tyrosine hydroxylase immunoreactivity shown in magenta (A, D, G, J, M) & synapsin immunoreactivity in green (B, E, H, K, N). Corresponding merged images in C, F, I, L, O. A-C: Frontal sections through lateral protocerebrum. 2 groups of TH-positive cell bodies (DCa1 & 2) labeled. DCa2 appears to send axons ventrally (arrowhead). Superior lateral protocerebrum (SLP) as well as medial & lateral calyces (MCA & LCA) innervated by TH-positive fibers. D-F: Frontal sections through vertical lobe (VL) & parts of medial calyx (MCA), which receive many fine TH-positive fibers. G-I: Horizontal sections through tip of vertical lobe & dorsal parts of calyces. Both calyces & vertical lobe innervated by fibrous TH-positive fibers. Note TH-immunoreactive fibers exclusively innervate synapsin-positive neuropil layers (NLs) of calyx where dendrites of Kenyon cells receive synaptic inputs from antennal-lobe projection neurons. J-L: Horizontal sections through calyces, ventral to panels G-I. Here, 3clusters of TH-positive cell bodies (DCa1, DCa2, & DSP1) visible. Immunoreactive fibers mainly invade anterior layers including gamma layer ( gamma ) in vertical lobe. M-O: Horizontal sections through distal portions of medial lobes (MLs), invaded by fine TH-positive fibers exhibiting moderate immunolabeling (arrows). in inferior medial protocerebrum (IMP) anterior to medial lobe, pair of DIP1 clusters (triangles) situated. D, dorsal; KFL, Kenyon fiber layer; LCA, lateral calyx; LO, lobula; M, medial; P, posterior; PED, pedunculus; SMP, superior medial protocerebrum. Scale bars = 100 μm. Image collected & cropped by CiteAb from following publication (//dx.plos.org/10.1371/journal.pone.0160531), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-109_rabbit-polyclonal-tyrosine-hydroxylase-antibody-310202416205113.jpg)
![Western Blot: Tyrosine Hydroxylase Antibody [NB300-110] - Analysis of rat caudate lysate showing specific immunolabeling of the ~60 kDa tyrosine hydroxylase protein.](http://images.novusbio.com/fullsize/Tyrosine-Hydroxylase-Antibody-Western-Blot-NB300-110-img0006.jpg "Western Blot: Tyrosine Hydroxylase Antibody [NB300-110] - Analysis of rat caudate lysate showing specific immunolabeling of the ~60 kDa tyrosine hydroxylase protein.")
![Western Blot: Tyrosine Hydroxylase Antibody [NB300-110] - Mice were injected with PBS (control group) or the dopaminergic neurotoxin MPTP. There is low concentration of TH in the striatum of mouse injected with MPTP. 30 ug total protein per lane. Antibody at 1:3000. Western blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Tyrosine-Hydroxylase-Antibody-Western-Blot-NB300-110-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Tyrosine Hydroxylase Antibody [NB300-110] - Alpha-Synuclein overexpression or intracerebral seeding impacts the dopaminergic system. Increased levels of ser129-phosphosynuclein in the substantia nigra (arrows) indicate alpha-syn stress in PFF injected animals. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30050065/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Tyrosine Hydroxylase Antibody-Immunocytochemistry-Immunofluorescence-NB300-110-img0013.jpg)
![Immunohistochemistry: Tyrosine Hydroxylase Antibody [NB300-110] - Mouse cryo-sections. Antibody at 1:500. Secondary antibody: dk-anti-sp Alexa Fluor488 at 1:500; 1 h. IHC image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Tyrosine-Hydroxylase-Antibody-Immunohistochemistry-NB300-110-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Tyrosine Hydroxylase Antibody [NB300-110] - Nmnat by itself does not protect dopaminergic neurons from MPP+ toxicity. Similar transduction efficiencies of the different lentiviruses were confirmed by quantifying the number of TH+ and GFP+ cells following transduction of dopaminergic cultures. Quantification of TH+ cell bodies and (E) TH+ neurites show that only WldS-transduced cultures protected neurites against MPP+. Data are normalized to control cultures and denote the mean +/- SEM of representative determinations made in three separate cultures. *p 99.8% posterior probability); green, expressed above baseline in human (>99.8% posterior probability); gray, not expressed above baseline.(F) Validation of LMO3 expression by immunohistochemistry in a subset of TH+ neurons in the E18.5 mouse ventral midbrain (scale bar left, 100 μm; right, 20 μm).(G) Validation of BNC2 expression by immunohistochemistry in TH+ neurons in the E18.5 mouse ventral midbrain (scale bar left, 100 μm; right, 20 μm). Image collected & cropped by CiteAb from the following publication (//linkinghub.elsevier.com/retrieve/pii/S0092867416313095), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-110_sheep-polyclonal-tyrosine-hydroxylase-antibody-310202416205145.jpg)
![Immunocytochemistry/ Immunofluorescence: Tyrosine Hydroxylase Antibody [NB300-110] - Developmental song exposure & social context affect cFOS expression in TH neurons of the caudal VTA. Sagittal drawings (A,C) & photomicrographs of tyrosine hydroxylase (TH; green label) expression (B,D) in the ventral tegmental area (VTA) & periaqueductal gray (PAG; panels A,B), & the substantia nigra pars compacta (SNc) & locus coeruleus (LC; panels C,D). (E) Examples of co-localized expression of TH (green) & cFOS (red) in the caudal VTA of a normally-reared bird that heard silence (left panel), UD song (middle panel; UD song) & FD song (FD; right panel). White arrows indicate examples of colocalized expression. (F) Percent of TH neurons co-localized with cFOS in the caudal VTA (cVTA), rostral VTA (rVTA) & SNc. Box-and-whisker plots for normally-reared (yellow colors) & song-naïve (green colors) hearing UD (UD; lighter colors) or female-directed (FD; darker colors) songs. Each box spans the interquartile range, horizontal white lines indicate the median & whiskers show the minima & maxima. Levels of cFOS expression in TH neurons for silence controls are plotted as the mean (gray dashed line) +/− standard error (gray boxes). *Indicates a significant difference at p 99.8% posterior probability); green, expressed above baseline in human (>99.8% posterior probability); gray, not expressed above baseline.(F) Validation of LMO3 expression by immunohistochemistry in a subset of TH+ neurons in the E18.5 mouse ventral midbrain (scale bar left, 100 μm; right, 20 μm).(G) Validation of BNC2 expression by immunohistochemistry in TH+ neurons in the E18.5 mouse ventral midbrain (scale bar left, 100 μm; right, 20 μm). Image collected & cropped by CiteAb from the following publication (//linkinghub.elsevier.com/retrieve/pii/S0092867416313095), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-110_sheep-polyclonal-tyrosine-hydroxylase-antibody-31020241621840.jpg)
![Immunocytochemistry/ Immunofluorescence: Tyrosine Hydroxylase Antibody [NB300-110] - Conversion of hNES cells into hPRogFPM & their differentiation into midbrain dopaminergic neurons. a Schematic representation of the conversion & differentiation protocols. b, cRT-qPCR analysis at day 8, showing the expression of midbrain-hindbrain TFs, such as OTX2, GBX2, LMX1A, & FOXA2 (b), as well as the dopaminergic neuron markers, NR4A2, TH, SLC18A22, & SLC6A3 (c). d Immunocytochemistry analysis of the presence of OTX2 & TH in control unconverted NES cultures, compared with NES cells converted with SAF+Dkk1 & differentiated until day 8. e, f Percentage of OTX2+ & TH+ cells in the conditions in d. P = 0.02673 (e), P = 0.03233 (f), n = 3. g–i Expression of the key midbrain TFs, LMX1A, NR4A2, & PBX1, in TH+cells derived from SAI2-NES cells after conversion & differentiation. j, k TH+ cells express the mature neuronal marker, MAP2 (j), & some acquire mature neuronal morphologies, with long processes & varicosities at day 8 (k). Scale 50μm. Box plots (b, c, e, f): Center line, median; hinges, 25% & 75% quartiles; whiskers, 1.5 interquartile range. Statistics: (b, c) ANOVA, followed by pair-wise t-test with Bonferroni correction for multiple testing. (e, f). Two sample t-test; *P ≤ 0,05; **P ≤ 0,01; ***P ≤ 0,001. N = 3 (GBX2, FOXA2, TH, SLC6A3), n = 4 (LMX1A, OTX2, NR4A2, SLC18A2) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29968757), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-110_sheep-polyclonal-tyrosine-hydroxylase-antibody-31020241621231.jpg)
![Immunocytochemistry/ Immunofluorescence: Tyrosine Hydroxylase Antibody [NB300-110] - Conversion of hNES cells into hPRogFPM & their differentiation into midbrain dopaminergic neurons. a Schematic representation of the conversion & differentiation protocols. b, cRT-qPCR analysis at day 8, showing the expression of midbrain-hindbrain TFs, such as OTX2, GBX2, LMX1A, & FOXA2 (b), as well as the dopaminergic neuron markers, NR4A2, TH, SLC18A22, & SLC6A3 (c). d Immunocytochemistry analysis of the presence of OTX2 & TH in control unconverted NES cultures, compared with NES cells converted with SAF+Dkk1 & differentiated until day 8. e, f Percentage of OTX2+ & TH+ cells in the conditions in d. P = 0.02673 (e), P = 0.03233 (f), n = 3. g–i Expression of the key midbrain TFs, LMX1A, NR4A2, & PBX1, in TH+cells derived from SAI2-NES cells after conversion & differentiation. j, k TH+ cells express the mature neuronal marker, MAP2 (j), & some acquire mature neuronal morphologies, with long processes & varicosities at day 8 (k). Scale 50μm. Box plots (b, c, e, f): Center line, median; hinges, 25% & 75% quartiles; whiskers, 1.5 interquartile range. Statistics: (b, c) ANOVA, followed by pair-wise t-test with Bonferroni correction for multiple testing. (e, f). Two sample t-test; *P ≤ 0,05; **P ≤ 0,01; ***P ≤ 0,001. N = 3 (GBX2, FOXA2, TH, SLC6A3), n = 4 (LMX1A, OTX2, NR4A2, SLC18A2) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29968757), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-110_sheep-polyclonal-tyrosine-hydroxylase-antibody-31020241621841.jpg)
![Immunocytochemistry/ Immunofluorescence: Tyrosine Hydroxylase Antibody [NB300-110] - Comparison of Mouse & Human Dopaminergic Neuronal Development(A) WNT1 compartments marking lateral population of the floor plate in human & mouse tissue (scale bar, 100 μm).(B) Bar plot of cell types of the human & mouse dopaminergic lineage, showing the expression of key genes. Bars show average mRNA expression, scaled to the absolute molecule counts indicated on the right axis. Error bars show SEM.(C) Validation of mNbM by in situ hybridization for Igfbpl1 & Nhlh1 (scale bar, 50 μm).(D) Neuroblasts in human & mouse ventral midbrain (scale bar, 100 μm; magnification, 20 μm).(E) Selected genes showing similar (left) or distinct (right) expression in mouse & human ventral midbrain. Blue, expressed above baseline in mouse (>99.8% posterior probability); green, expressed above baseline in human (>99.8% posterior probability); gray, not expressed above baseline.(F) Validation of LMO3 expression by immunohistochemistry in a subset of TH+ neurons in the E18.5 mouse ventral midbrain (scale bar left, 100 μm; right, 20 μm).(G) Validation of BNC2 expression by immunohistochemistry in TH+ neurons in the E18.5 mouse ventral midbrain (scale bar left, 100 μm; right, 20 μm). Image collected & cropped by CiteAb from the following publication (//linkinghub.elsevier.com/retrieve/pii/S0092867416313095), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-110_sheep-polyclonal-tyrosine-hydroxylase-antibody-31020241621827.jpg)
![Immunocytochemistry/ Immunofluorescence: Tyrosine Hydroxylase Antibody [NB300-110] - alpha -Synuclein overexpression or intracerebral seeding impacts the dopaminergic system.a–c Intranigral injection of AAV2/6 serotype expressing human alpha -syn (h-syn) results in increased protein expression paralleled by reduction in tyrosine hydroxylase (TH) levels. (d) h-syn is also expressed in dopaminergic cell bodies (arrows). e Unbiased stereological counts demonstrate a reduction in nigral dopaminergic cell bodies. f, g Intracranial injection of alpha -syn pre-formed fibrils (PFF) causes striatal dopaminergic denervation as evidenced by a reduction in TH immunoreactivity. h Increased levels of ser129-phosphosynuclein in the substantia nigra (arrows) indicate alpha -syn stress in PFF injected animals. i Unbiased stereological counts showing a decrease in dopaminergic cell bodies in the substantia nigra. Scale bars: 1 mm in a, b 50 μm in d. (**p < 0.01; ***p < 0.001; Student’s t test). All bar graphs show mean + /− s.e.m Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30050065), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-110_sheep-polyclonal-tyrosine-hydroxylase-antibody-310202416212352.jpg)
![Tyrosine Hydroxylase [p Ser40] Antibody [NB300-173] - Western blot of recombinant phospho-TH and non-phospho-TH showing selective immunolabeling by the phosphospecific antibody of the ~60 kDa TH phosphorylated at Ser40. The pan-specific antibody (anti-pan-TH) recognized both the phospho- and non-phospho-TH; while most importantly, the phospho-specific antibody (anti-Ser40 TH) recognized only phospho-TH.](http://images.novusbio.com/fullsize/Tyrosine-Hydroxylase-[p-Ser40]-Antibody-NB300-173-img0005.jpg "Tyrosine Hydroxylase [p Ser40] Antibody [NB300-173] - Western blot of recombinant phospho-TH and non-phospho-TH showing selective immunolabeling by the phosphospecific antibody of the ~60 kDa TH phosphorylated at Ser40. The pan-specific antibody (anti-pan-TH) recognized both the phospho- and non-phospho-TH; while most importantly, the phospho-specific antibody (anti-Ser40 TH) recognized only phospho-TH.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry: Tyrosine Hydroxylase [p Ser40] Antibody [NB300-173] - Immunohistochemical staining of retina with the pan-tyrosine hydroxylase (pan-TH) and phospho-specific tyrosine hydroxylase (phospho-TH) antibodies. The pan-TH antibody shows extensive labeling in this photomicrograph of the retina. In contrast, the phospho-TH antibody selectively labels only the two amacrine cells in this light-stimulated retina example.](http://images.novusbio.com/fullsize/Tyrosine-Hydroxylase-[p-Ser40]-Antibody-Immunohistochemistry-NB300-173-img0004.jpg)
![Western Blot: Tyrosine Hydroxylase [p Ser31] Antibody [NB300-211] - PC-12 cells incubated in the absence (Control) and presence of okadaic acid (OA, 1 uM for 60 min) showing specific immunolabeling of the ~60 kDa TH phosphorylated at Ser31.](http://images.novusbio.com/fullsize/Tyrosine-Hydroxylase-[p-Ser31]-Antibody-Western-Blot-NB300-211-img0001.jpg "Western Blot: Tyrosine Hydroxylase [p Ser31] Antibody [NB300-211] - PC-12 cells incubated in the absence (Control) and presence of okadaic acid (OA, 1 uM for 60 min) showing specific immunolabeling of the ~60 kDa TH phosphorylated at Ser31.")
![Immunohistochemistry-Paraffin: Tyrosine Hydroxylase Antibody [NBP2-38903] - Analysis in human adrenal gland and stomach tissues. Corresponding TH RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Tyrosine-Hydroxylase-Antibody-Immunohistochemistry-Paraffin-NBP2-38903-img0013.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Tyrosine Hydroxylase Antibody [NBP2-38903] - Analysis in human adrenal gland and stomach tissues. Corresponding TH RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry: Tyrosine Hydroxylase Antibody [NBP2-38903] - Staining of mouse midbrain shows positive processes and cell bodies.](http://images.novusbio.com/fullsize/Tyrosine-Hydroxylase-Antibody-Immunohistochemistry-NBP2-38903-img0018.jpg)
![Immunohistochemistry-Paraffin: Tyrosine Hydroxylase Antibody [NBP2-38903] - Staining of human adrenal gland shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Tyrosine-Hydroxylase-Antibody-Immunohistochemistry-Paraffin-NBP2-38903-img0019.jpg)
![Immunohistochemistry-Paraffin: Tyrosine Hydroxylase Antibody [NBP2-38903] - Staining of human caudate shows moderate positivity in neuropil.](http://images.novusbio.com/fullsize/Tyrosine-Hydroxylase-Antibody-Immunohistochemistry-Paraffin-NBP2-38903-img0020.jpg)
![Immunohistochemistry-Paraffin: Tyrosine Hydroxylase Antibody [NBP2-38903] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/Tyrosine-Hydroxylase-Antibody-Immunohistochemistry-Paraffin-NBP2-38903-img0021.jpg)
![Immunohistochemistry-Paraffin: Tyrosine Hydroxylase Antibody [NBP2-38903] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Tyrosine-Hydroxylase-Antibody-Immunohistochemistry-Paraffin-NBP2-38903-img0022.jpg)
![Immunohistochemistry-Paraffin: Tyrosine Hydroxylase Antibody [NBP2-38903] - Staining of human stomach shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/Tyrosine-Hydroxylase-Antibody-Immunohistochemistry-Paraffin-NBP2-38903-img0023.jpg)
![Immunohistochemistry: Tyrosine Hydroxylase Antibody [NBP2-38903] - Staining of mouse amygdala shows positive processes.](http://images.novusbio.com/fullsize/Tyrosine-Hydroxylase-Antibody-Immunohistochemistry-NBP2-38903-img0014.jpg)
![Immunohistochemistry: Tyrosine Hydroxylase Antibody [NBP2-38903] - Staining of mouse caudate putamen shows synaptic immunoreactivity.](http://images.novusbio.com/fullsize/Tyrosine-Hydroxylase-Antibody-Immunohistochemistry-NBP2-38903-img0015.jpg)
![Immunohistochemistry: Tyrosine Hydroxylase Antibody [NBP2-38903] - Staining of mouse hindbrain shows positivity in the locus coeruleus.](http://images.novusbio.com/fullsize/Tyrosine-Hydroxylase-Antibody-Immunohistochemistry-NBP2-38903-img0016.jpg)
![Immunohistochemistry: Tyrosine Hydroxylase Antibody [NBP2-38903] - Staining of mouse hypothalamus shows immunoreactivity in a subset of neurons in the paraventricular nucleus.](http://images.novusbio.com/fullsize/Tyrosine-Hydroxylase-Antibody-Immunohistochemistry-NBP2-38903-img0017.jpg)
![Western Blot: Tyrosine Hydroxylase Antibody [NBP3-05555] - Western blot analysis of different tissue lysates using Tyrosine Hydroxylase Antibody at a dilution of 1:50,000 in green: [1] protein standard (red), [2] rat brain caudate/putmen region, [3] rat brain midbrain, [4] whole mouse brain and brain stem excluding cerebellum, and [5] segment of cow midbrain. Strong band at about 58kDa corresponds to the Tyrosine Hydroxylase protein.](http://images.novusbio.com/fullsize/Tyrosine-Hydroxylase-Antibody-Western-Blot-NBP3-05555-img0002.jpg "Western Blot: Tyrosine Hydroxylase Antibody [NBP3-05555] - Western blot analysis of different tissue lysates using Tyrosine Hydroxylase Antibody at a dilution of 1:50,000 in green: [1] protein standard (red), [2] rat brain caudate/putmen region, [3] rat brain midbrain, [4] whole mouse brain and brain stem excluding cerebellum, and [5] segment of cow midbrain. Strong band at about 58kDa corresponds to the Tyrosine Hydroxylase protein.")
![Immunocytochemistry/Immunofluorescence: Tyrosine Hydroxylase Antibody [NBP3-05555] - Immunofluorescent analysis of rat brain section stained with Tyrosine Hydroxylase Antibody at a dilution of 1:5,000, in green. The blue is Hoechst staining of nuclear DNA. Tyrosine Hydroxylase Antibody stains the dopaminergic TH-positive neurons and their processes of the substantia nigra.](http://images.novusbio.com/fullsize/Tyrosine-Hydroxylase-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05555-img0001.jpg)
![Immunohistochemistry: Tyrosine Hydroxylase Antibody (CL3049) [NBP2-46647] - Staining of mouse brain shows strong positivity in neurons in the hypothalamus.](http://images.novusbio.com/fullsize/Tyrosine-Hydroxylase-Antibody-CL3049-Immunohistochemistry-NBP2-46647-img0010.jpg "Immunohistochemistry: Tyrosine Hydroxylase Antibody (CL3049) [NBP2-46647] - Staining of mouse brain shows strong positivity in neurons in the hypothalamus.")
![Immunohistochemistry: Tyrosine Hydroxylase Antibody (3049) [NBP2-46647] - Staining of human caudate nucleus shows immunoreactivity in monoaminergic neural fibers.](http://images.novusbio.com/fullsize/Tyrosine-Hydroxylase-Antibody-3049-Immunohistochemistry-NBP2-46647-img0003.jpg)
![Immunohistochemistry: Tyrosine Hydroxylase Antibody (3049) [NBP2-46647] - Staining of human prostate shows strong immunoreactivity in sympathetic fibers.](http://images.novusbio.com/fullsize/Tyrosine-Hydroxylase-Antibody-3049-Immunohistochemistry-NBP2-46647-img0006.jpg)
![Immunohistochemistry: Tyrosine Hydroxylase Antibody (3049) [NBP2-46647] - Analysis of human kidney shows absence of immunoreactivity (negative control).](http://images.novusbio.com/fullsize/Tyrosine-Hydroxylase-Antibody-3049-Immunohistochemistry-NBP2-46647-img0007.jpg)
![Immunohistochemistry: Tyrosine Hydroxylase Antibody (3049) [NBP2-46647] - Immunohistochemical staining of rat locus coeruleus using Tyrosine Hydroxylase antibody (clone 3049) with immunofluorescence based detection showing a very strong cytoplasmic immunoreactivity in monoaminergic neurons.](http://images.novusbio.com/fullsize/Tyrosine-Hydroxylase-Antibody-3049-Immunohistochemistry-NBP2-46647-img0008.jpg)
![Immunohistochemistry: Tyrosine Hydroxylase Antibody (CL3049) [NBP2-46647] - Staining of rat locus coeruleus shows strong positivity in noradrenergic neurons.](http://images.novusbio.com/fullsize/Tyrosine-Hydroxylase-Antibody-CL3049-Immunohistochemistry-NBP2-46647-img0009.jpg)
![Western Blot: Recombinant Human Tyrosine Hydroxylase His Protein [NBP1-42461] - Novus Biologicals antibody NB300-109 was used to detect the protein on a Western blot. 150 ng of protein were added to the Western blot.](http://images.novusbio.com/fullsize/Tyrosine-Hydroxylase-Protein-Western-Blot-NBP1-42461-img0002.jpg "Western Blot: Recombinant Human Tyrosine Hydroxylase His Protein [NBP1-42461] - Novus Biologicals antibody NB300-109 was used to detect the protein on a Western blot. 150 ng of protein were added to the Western blot.")
![SDS-Page: Recombinant Human Tyrosine Hydroxylase His Protein [NBP1-42461] - This product has a purity >95%. This protein was run on a 4-20% SDS-PAGE gel with 5 ug protein loaded.](http://images.novusbio.com/fullsize/Tyrosine-Hydroxylase-Protein-Immunoblotting-NBP1-42461-img0001.jpg)
![ELISA: Human Tyrosine Hydroxylase ELISA Kit (Colorimetric) [NBP3-06920] - Samples were spiked with high concentrations of Human Tyrosine Hydroxylase and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp3-06920_human-tyrosine-hydroxylase-elisa-kit-colorimetric-1322024168531.jpg "ELISA: Human Tyrosine Hydroxylase ELISA Kit (Colorimetric) [NBP3-06920] - Samples were spiked with high concentrations of Human Tyrosine Hydroxylase and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human Tyrosine Hydroxylase ELISA Kit (Colorimetric) [NBP3-06920] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-06920_human-tyrosine-hydroxylase-elisa-kit-colorimetric-432024857426.jpg)