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![Simple Western: mtTFA Antibody (18G102B2E11) [NBP1-71648] - Simple Western lane view shows a specific band for mtTFA in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/mtTFA-Antibody-18G102B2E11-Simple-Western-NBP1-71648-img0008.jpg "Simple Western: mtTFA Antibody (18G102B2E11) [NBP1-71648] - Simple Western lane view shows a specific band for mtTFA in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![<b>Genetic Strategies Validation. </b>Knockdown Validated: mtTFA Antibody (18G102B2E11) [NBP1-71648] - MtDNA depletion and reversible impaired immune functions in THP-1A cells. A and B, Treatment with 50A ng/mL ethidium bromide (EtBr) for 8A weeks. Transfection with 30A nmol/L negative or TFAM siRNA for 8A days. TFAM protein relative toI2-actin. D, MtDNA levels. E, LPS-induced TNF-I+-and IL-8 release. Image collected and cropped by Citeab from the following publication (Mitochondrial DNA depletion induces innate immune dysfunction rescued by IFN-g. J Allergy Clin Immunol (2017)) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/mtTFA-Antibody-18G102B2E11-Western-Blot-NBP1-71648-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: mtTFA Antibody (18G102B2E11) [NBP1-71648] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-mtTFA Antibody (18G102B2E11) NBP1-71648 at 1 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/mtTFA-Antibody-18G102B2E11-Immunocytochemistry-Immunofluorescence-NBP1-71648-img0014.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: mtTFA Antibody (18G102B2E11) [NBP1-71648] - Induction of mitochondrial biogenesis following exposure of THP-1 cells to LPS. THP-1 cells were incubated with LPS (100 ng/ml) for 0AE](http://images.novusbio.com/fullsize/mtTFA-Antibody-18G102B2E11-Western-Blot-NBP1-71648-img0013.jpg)
![Immunohistochemistry-Paraffin: mtTFA Antibody (18G102B2E11) [NBP1-71648] - IHC staining of mtTFA on human testis using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/mtTFA-Antibody-18G102B2E11-Immunohistochemistry-Paraffin-NBP1-71648-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: mtTFA Antibody (18G102B2E11) [NBP1-71648] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-mtTFA Antibody (18G102B2E11) NBP1-71648 at 1 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/mtTFA-Antibody-18G102B2E11-Immunocytochemistry-Immunofluorescence-NBP1-71648-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: mtTFA Antibody (18G102B2E11) [NBP1-71648] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-mtTFA Antibody (18G102B2E11) NBP1-71648 at 1 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/mtTFA-Antibody-18G102B2E11-Immunocytochemistry-Immunofluorescence-NBP1-71648-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: mtTFA Antibody (18G102B2E11) [NBP1-71648] - Immunostaining in HeLa cells. C1: DAPI staining 2 nuclei, C2: anti-mtTFA stain with Alexa Fluor 488 secondary ab (green), C3: Mitotracker Red staining mitochondria. Image courtesy of Elizabeth Wang.](http://images.novusbio.com/fullsize/mtTFA-Antibody-18G102B2E11-Immunocytochemistry-Immunofluorescence-NBP1-71648-img0003.jpg)
![Western Blot: mtTFA Antibody (18G102B2E11) [NBP1-71648] - Total protein from Hela and U937 were separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-mtTFA NBP1-71648 in blocking buffer and detected with an anti-mouse HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).](http://images.novusbio.com/fullsize/mtTFA-Antibody-18G102B2E11-Western-Blot-NBP1-71648-img0017.jpg)
![Western Blot: mtTFA Antibody (18G102B2E11) [NBP1-71648] - Analysis of mtTFA expression in HeLa whole cell lysate.](http://images.novusbio.com/fullsize/mtTFA-Antibody-18G102B2E11-Western-Blot-NBP1-71648-img0006.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: mtTFA Antibody (18G102B2E11) [NBP1-71648] - Alcohol feeding significantly decreased TFAM expression in rat liver. This image was submitted by a customer review.](http://images.novusbio.com/fullsize/mtTFA-Antibody-18G102B2E11-Western-Blot-NBP1-71648-img0009.jpg)
![Western Blot: mtTFA Antibody (18G102B2E11) [NBP1-71648] - MtDNA depletion & reversible impaired immune functions in THP-1 cells. A & B, Treatment with 50 ng/mL ethidium bromide (EtBr) for 8 weeks. A, MtDNA levels. B, LPS-induced TNF-alpha & IL-8 release. C-E, Transfection with 30 nmol/L negative or TFAM siRNA for 8 days. C, TFAM protein relative to beta -actin. D, MtDNA levels. E, LPS-induced TNF-alpha & IL-8 release. F-H, TFAM recovery 8 days after removal of TFAM siRNA. F, TFAM protein relative beta -actin. G, MtDNA levels. H, LPS-induced TNF-alpha & IL-8 release. All experiments are presented as means ± SD of 3 to 4 independent biological replicates. *P < .05, **P < .01, & ***P < .001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28629747), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-71648_mouse-monoclonal-mttfa-antibody-18g102b2e11-310202415384163.jpg)
![Western Blot: mtTFA Antibody (18G102B2E11) [NBP1-71648] - MtDNA depletion & impaired immune functions & subsequent recovery in THP-1 cells following transfection with TFAM siRNA. A-G, Transfection with TFAM siRNA. A, TFAM proteins levels relative to beta -actin during titration of TFAM siRNA, showing optimal knockdown of TFAM protein after transfection of THP-1 cells with 30 nmol/L siRNA for 8 days. B, Cell viability. C, Cell proliferation. D, The levels of the MT-CO1 & SDHA proteins relative to beta -actin. D, OCR for different aspects of mitochondrial respiration & respiratory profile. E, Phagocytosis of E coli. Recovery 8 days after removal of TFAM siRNA. F, Levels of the MT-CO1 & SDHA proteins relative to beta -actin. G, Oxygen consumption for different aspects of mitochondrial respiration. H, Bacterial phagocytosis. All experiments were carried out on 3 to 4 independent biological replicates & are presented as means ± SD. **P < .01 & ***P < .001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28629747), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-71648_mouse-monoclonal-mttfa-antibody-18g102b2e11-310202416182618.jpg)
![Western Blot: mtTFA Antibody [NBP2-19437] - Mitochondrial-related protein expression in LA tissue. Representative Western blot analysis of the protein expression in TFAM. Data is mean+/-SD, relative protein level was normalized to beta-actin. *Compared with the control group P<0.05. #Compared with the DM group P<0.05. DM indicates diabetes mellitus; NS, not significant; TFAM, mitochondrial transcription factor A. Image collected and cropped by CiteAb from the following publication (www.ahajournals.org/doi/10.1161/JAHA.118.008807) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/mtTFA-Antibody-Western-Blot-NBP2-19437-img0008.jpg "Western Blot: mtTFA Antibody [NBP2-19437] - Mitochondrial-related protein expression in LA tissue. Representative Western blot analysis of the protein expression in TFAM. Data is mean+/-SD, relative protein level was normalized to beta-actin. *Compared with the control group P<0.05. #Compared with the DM group P<0.05. DM indicates diabetes mellitus; NS, not significant; TFAM, mitochondrial transcription factor A. Image collected and cropped by CiteAb from the following publication (www.ahajournals.org/doi/10.1161/JAHA.118.008807) licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: mtTFA Antibody [NBP2-19437] - Human colon cancer. mtTFA antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/mtTFA-Antibody-Immunohistochemistry-Paraffin-NBP2-19437-img0007.jpg)
![Immunohistochemistry-Paraffin: mtTFA Antibody [NBP2-19437] - Human esophagus cancer. mtTFA antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/mtTFA-Antibody-Immunohistochemistry-Paraffin-NBP2-19437-img0006.jpg)
![Immunoprecipitation: mtTFA Antibody [NBP2-19437] - A. 293T whole cell lysate/extract B. 40 ug 293T whole cell lysate/extract. C.. Control with 2 ug of preimmune rabbit IgG.](http://images.novusbio.com/fullsize/mtTFA-Antibody-Immunoprecipitation-NBP2-19437-img0004.jpg)
![HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: mtTFA stained by mtTFA antibody diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:1000.Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/antibody/nbp2-19437_rabbit-polyclonal-mttfa-antibody-immunofluorescence-22320239914.jpg)
![Western Blot: mtTFA Antibody [NBP2-19437] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with mtTFA antibody (NBP2-19437) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19437_rabbit-polyclonal-mttfa-antibody-910202420115071.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: mtTFA Antibody [NBP2-19437] - Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with mtTFA antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19437_rabbit-polyclonal-mttfa-antibody-1610202419414569.jpg)
![Immunocytochemistry/Immunofluorescence: mtTFA Antibody [NBP2-19438] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TFAM stained by TFAM antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/mtTFA-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19438-img0012.jpg "Immunocytochemistry/Immunofluorescence: mtTFA Antibody [NBP2-19438] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TFAM stained by TFAM antibody diluted at 1:1000.")
![<b>Genetic Strategies Validation. </b>Western Blot: mtTFA Antibody [NBP2-19438] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with mtTFA antibody diluted at 1:3000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/mtTFA-Antibody-Western-Blot-NBP2-19438-img0011.jpg)
![Immunohistochemistry-Paraffin: mtTFA Antibody [NBP2-19438] - Human breast cancer, using mtTFA antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/mtTFA-Antibody-Immunohistochemistry-Paraffin-NBP2-19438-img0009.jpg)
![Western Blot: mtTFA Antibody [NBP2-19438] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with mtTFA antibody ) diluted at 1:1000.](http://images.novusbio.com/fullsize/mtTFA-Antibody-Western-Blot-NBP2-19438-img0003.jpg)
![Western Blot: mtTFA Antibody [NBP2-19438] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with mtTFA antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/mtTFA-Antibody-Western-Blot-NBP2-19438-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: mtTFA Antibody [NBP2-19438] - NT2D1 cells were fixed in 2% paraformaldehyde/culture medium at RT for 30 min. Green: mtTFA protein stained by mtTFA antibody diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 um.](http://images.novusbio.com/fullsize/mtTFA-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19438-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: mtTFA Antibody [NBP2-19438] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: mtTFA protein stained by mtTFA antibody diluted at 1:500. Red: Mitotracker, a mitochondria marker, diluted at 1:2000. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/mtTFA-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19438-img0006.jpg)
![Immunoprecipitation: mtTFA Antibody [NBP2-19438] - IP Sample: 293T whole cell lysate/extract A. 40 ug 293T whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of mtTFA protein by 2 ug of mtTFA antibody 12% SDS-PAGE The immunoprecipitated mtTFA protein was detected by mtTFA antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/mtTFA-Antibody-Immunoprecipitation-NBP2-19438-img0008.jpg)
![Western Blot: mtTFA Antibody [NBP2-19438] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with mtTFA antibody (NBP2-19438) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19438_rabbit-polyclonal-mttfa-antibody-9102024205611.jpg)
![Immunohistochemistry-Paraffin: mtTFA Antibody [NBP2-19438] - mtTFA antibody detects mtTFA protein at mitochondria by immunohistochemical analysis.Sample: Paraffin-embedded human esophageal carcinoma.mtTFA stained by mtTFA antibody (NBP2-19438) diluted at 1:165.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-19438_rabbit-polyclonal-mttfa-antibody-91020242014635.jpg)
![Western Blot: mtTFA Antibody [NBP1-86959] - Analysis using Anti-TFAM antibody NBP1-86959 (A) shows similar pattern to independent antibody NBP2-56077 (B).](http://images.novusbio.com/fullsize/mtTFA-Antibody-Western-Blot-NBP1-86959-img0016.jpg "<b>Independent Antibodies Validation. </b>Western Blot: mtTFA Antibody [NBP1-86959] - Analysis using Anti-TFAM antibody NBP1-86959 (A) shows similar pattern to independent antibody NBP2-56077 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: mtTFA Antibody [NBP1-86959] - Staining of human cell line U-2 OS shows positivity in mitochondria.](http://images.novusbio.com/fullsize/mtTFA-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86959-img0014.jpg)
![Immunohistochemistry-Paraffin: mtTFA Antibody [NBP1-86959] - Staining of human testis shows strong granular cytoplasmic positivity in a subset of cells in seminiferous ducts.](http://images.novusbio.com/fullsize/mtTFA-Antibody-Immunohistochemistry-Paraffin-NBP1-86959-img0013.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: mtTFA Antibody [NBP1-86959] - Analysis in human cell lines Caco-2 and HeLa using anti-TFAM antibody. Corresponding TFAM RNA-seq data are presented for the same cell lines. Loading control: anti-PARP1.](http://images.novusbio.com/fullsize/mtTFA-Antibody-Western-Blot-NBP1-86959-img0015.jpg)
![Immunohistochemistry-Paraffin: mtTFA Antibody [NBP1-86959] - Staining of human breast shows strong cytoplasmic positivity in granular pattern in glandular cells.](http://images.novusbio.com/fullsize/mtTFA-Antibody-Immunohistochemistry-Paraffin-NBP1-86959-img0007.jpg)
![Immunohistochemistry-Paraffin: mtTFA Antibody [NBP1-86959] - Staining of human duodenum shows moderate granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/mtTFA-Antibody-Immunohistochemistry-Paraffin-NBP1-86959-img0010.jpg)
![Immunohistochemistry-Paraffin: mtTFA Antibody [NBP1-86959] - Staining of human kidney shows moderate granular cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/mtTFA-Antibody-Immunohistochemistry-Paraffin-NBP1-86959-img0011.jpg)
![Immunohistochemistry-Paraffin: mtTFA Antibody [NBP1-86959] - Staining of human placenta shows moderate granular cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/mtTFA-Antibody-Immunohistochemistry-Paraffin-NBP1-86959-img0012.jpg)
![Western Blot: mtTFA Antibody (8O1U0) [NBP3-16154] - Western blot analysis of extracts of various cell lines, using mtTFA Rabbit mAb (NBP3-16154) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/mtTFA-Antibody-8O1U0-Western-Blot-NBP3-16154-img0003.jpg "Western Blot: mtTFA Antibody (8O1U0) [NBP3-16154] - Western blot analysis of extracts of various cell lines, using mtTFA Rabbit mAb (NBP3-16154) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunocytochemistry/Immunofluorescence: mtTFA Antibody (8O1U0) [NBP3-16154] - Immunofluorescence analysis of U-2 OS cells using mtTFA Rabbit mAb (NBP3-16154) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/mtTFA-Antibody-8O1U0-Immunocytochemistry-Immunofluorescence-NBP3-16154-img0002.jpg)
![Immunohistochemistry-Paraffin: mtTFA Antibody (8O1U0) [NBP3-16154] - Immunohistochemistry of paraffin-embedded human colon using mtTFA Rabbit mAb (NBP3-16154) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/mtTFA-Antibody-8O1U0-Immunohistochemistry-Paraffin-NBP3-16154-img0001.jpg)
![Chromatin Immunoprecipitation: mtTFA Antibody (8O1U0) [NBP3-16154] - Chromatin immunoprecipitation analysis of extracts of K-562 cells, using mtTFA antibody (NBP3-16154) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/mtTFA-Antibody-8O1U0-Chromatin-Immunoprecipitation-NBP3-16154-img0004.jpg)
![Western Blot: mtTFA Antibody (S08-3E2) [NBP3-19504] - Western blot detection of mtTFA in K562, Hela cell lysates using NBP3-19504 (1:1000 diluted). Predicted band size: 29kDa. Observed band size: 24kDa.](http://images.novusbio.com/fullsize/mtTFA-Antibody-S08-3E2-Western-Blot-NBP3-19504-img0003.jpg "Western Blot: mtTFA Antibody (S08-3E2) [NBP3-19504] - Western blot detection of mtTFA in K562, Hela cell lysates using NBP3-19504 (1:1000 diluted). Predicted band size: 29kDa. Observed band size: 24kDa.")
![Immunocytochemistry/Immunofluorescence: mtTFA Antibody (S08-3E2) [NBP3-19504] - Immunofluorescence of mtTFA (green) in Hela cells using NBP3-19504 at dilution 1/200, and DAPI (blue)](http://images.novusbio.com/fullsize/mtTFA-Antibody-S08-3E2-Immunocytochemistry-Immunofluorescence-NBP3-19504-img0001.jpg)
![Immunohistochemistry-Paraffin: mtTFA Antibody (S08-3E2) [NBP3-19504] - Immunohistochemistry of mtTFA in paraffin-embedded Human breast cancer tissue using NBP3-19504 at dilution 1/1](http://images.novusbio.com/fullsize/mtTFA-Antibody-S08-3E2-Immunohistochemistry-Paraffin-NBP3-19504-img0002.jpg)
![Western Blot: mtTFA Antibody [NBP2-56077] - Analysis in human cell lines Caco-2 and HeLa. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/mtTFA-Antibody-Western-Blot-NBP2-56077-img0003.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: mtTFA Antibody [NBP2-56077] - Analysis in human cell lines Caco-2 and HeLa. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Western Blot: mtTFA Antibody [NBP2-56077] - Analysis using Anti-TFAM antibody NBP2-56077 (A) shows similar pattern to independent antibody NBP1-86959 (B).](http://images.novusbio.com/fullsize/mtTFA-Antibody-Western-Blot-NBP2-56077-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: mtTFA Antibody [NBP2-56077] - Staining of human cell line RH-30 shows localization to mitochondria.](http://images.novusbio.com/fullsize/mtTFA-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56077-img0001.jpg)
![SDS-Page: Recombinant Human mtTFA Protein [NBP1-50977] - 15% SDS-PAGE (3ug)](http://images.novusbio.com/fullsize/Recombinant-Human-mtTFA-Protein-SDS-Page-NBP1-50977-img0002.jpg "SDS-Page: Recombinant Human mtTFA Protein [NBP1-50977] - 15% SDS-PAGE (3ug)")
![ELISA: Human mtTFA ELISA Kit (Colorimetric) [NBP3-38686] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-38686_human-mttfa-elisa-kit-colorimetric-2110202415295690.png "ELISA: Human mtTFA ELISA Kit (Colorimetric) [NBP3-38686] - Standard Curve Reference")
![ELISA: Human mtTFA - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-38685] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-38685_human-mttfa-ready-to-use-elisa-kit-colorimetric-211020241529170.png "ELISA: Human mtTFA - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-38685] - Standard Curve Reference")