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![Western Blot: TEF1 Antibody [NBP2-94035] - Analysis of extracts of various cell lines, using TEAD1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/TEF1-Antibody-Western-Blot-NBP2-94035-img0012.jpg "Western Blot: TEF1 Antibody [NBP2-94035] - Analysis of extracts of various cell lines, using TEAD1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/Immunofluorescence: TEF1 Antibody [NBP2-94035] - Analysis of MCF7 cells using TEF1 .](http://images.novusbio.com/fullsize/TEF1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-94035-img0002.jpg)
![Immunohistochemistry-Paraffin: TEF1 Antibody [NBP2-94035] - Mouse heart using TEAD1 Rabbit pAb at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/TEF1-Antibody-Immunohistochemistry-Paraffin-NBP2-94035-img0015.jpg)
![Chromatin Immunoprecipitation: TEF1 Antibody [NBP2-94035] - Analysis of extracts of HeLa cell line, using TEF1 rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/TEF1-Antibody-Chromatin-Immunoprecipitation-NBP2-94035-img0001.jpg)
![Western Blot: TEF1 Antibody [NBP2-94035] - Analysis of extracts of various cell lines, using TEAD1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/TEF1-Antibody-Western-Blot-NBP2-94035-img0011.jpg)
![Immunohistochemistry-Paraffin: TEF1 Antibody [NBP2-94035] - Rat brain using TEAD1 Rabbit pAb at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/TEF1-Antibody-Immunohistochemistry-Paraffin-NBP2-94035-img0013.jpg)
![Immunohistochemistry-Paraffin: TEF1 Antibody [NBP2-94035] - Human placenta using TEAD1 Rabbit pAb at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/TEF1-Antibody-Immunohistochemistry-Paraffin-NBP2-94035-img0014.jpg)
![Western Blot: TEF1 Antibody [NBP2-95204] - Analysis of extracts of various cell lines, using TEF1 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 120s.](http://images.novusbio.com/fullsize/TEF1-Antibody-Western-Blot-NBP2-95204-img0003.jpg "Western Blot: TEF1 Antibody [NBP2-95204] - Analysis of extracts of various cell lines, using TEF1 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 120s.")
![Immunocytochemistry/Immunofluorescence: TEF1 Antibody [NBP2-95204] - Analysis of MCF7 cells using TEF1 .](http://images.novusbio.com/fullsize/TEF1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-95204-img0002.jpg)
![Chromatin Immunoprecipitation: TEF1 Antibody [NBP2-95204] - Analysis of extracts of HeLa cells, using TEF1 rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/TEF1-Antibody-Chromatin-Immunoprecipitation-NBP2-95204-img0001.jpg)
![Western Blot: TEF1 Antibody (2E7N10) [NBP3-16593] - Western blot analysis of extracts of various cell lines, using TEF1 Rabbit mAb (NBP3-16593) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/TEF1-Antibody-2E7N10-Western-Blot-NBP3-16593-img0001.jpg "Western Blot: TEF1 Antibody (2E7N10) [NBP3-16593] - Western blot analysis of extracts of various cell lines, using TEF1 Rabbit mAb (NBP3-16593) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunoprecipitation: TEF1 Antibody (2E7N10) [NBP3-16593] - Immunoprecipitation analysis of 600ug extracts of Mouse skeletal muscle cells using 3ug TEF1 antibody (NBP3-16593). Western blot was performed from the immunoprecipitate using TEF1 antibody (NBP3-16593) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/TEF1-Antibody-2E7N10-Immunoprecipitation-NBP3-16593-img0002.jpg)
![Chromatin Immunoprecipitation: TEF1 Antibody (2E7N10) [NBP3-16593] - Analysis of extracts of HepG2 cells, using TEAD1 antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/nbp3-16593_rabbit-tef1-mab-2e7n10-chromatin-immunoprecipitation-432024122010..jpg)
![Immunocytochemistry/Immunofluorescence: TEF1 Antibody (13112) [NBP2-43581] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TEF1 protein stained by TEF1 antibody [GT13112] diluted at 1:400. Red: Phalloidin, a F-actin marker, diluted at 1:200.](http://images.novusbio.com/fullsize/TEF1-Antibody-13112-Immunocytochemistry-Immunofluorescence-NBP2-43581-img0003.jpg "Immunocytochemistry/Immunofluorescence: TEF1 Antibody (13112) [NBP2-43581] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TEF1 protein stained by TEF1 antibody [GT13112] diluted at 1:400. Red: Phalloidin, a F-actin marker, diluted at 1:200.")
![Immunoprecipitation: TEF1 Antibody (13112) [NBP2-43581] - Immunoprecipitation of TEF1 protein from HeLa nuclear extracts using 5 ug of TEF1 antibody [13112]. Western blot analysis was performed using TEF1 antibody [13112] EasyBlot anti-Mouse IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/TEF1-Antibody-13112-Immunoprecipitation-NBP2-43581-img0007.jpg)
![Western Blot: TEAD1 Antibody (13112) [NBP2-43581] - HeLa whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TEAD1 antibody [GT13112] (NBP2-43581) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43581_mouse-monoclonal-tead1-antibody-13112-9102024202463.jpg)
![Western Blot: TEF1 Antibody (JE45-37) [NBP3-33004] - Western blot analysis of TEF1 on different lysates with Rabbit anti-TEF1 antibody (NBP3-33004) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: A549 cell lysate Lane 3: 293T cell lysate Lane 4: SiHa cell lysate Lane 5: C2C12 cell lysate Lane 6: L6 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 48 kDa Observed band size: 48/50 kDa Exposure time: 3 minutes; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33004) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33004_rabbit-tef1-mab-je45-37-288202412493481.jpg "Western Blot: TEF1 Antibody (JE45-37) [NBP3-33004] - Western blot analysis of TEF1 on different lysates with Rabbit anti-TEF1 antibody (NBP3-33004) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: A549 cell lysate Lane 3: 293T cell lysate Lane 4: SiHa cell lysate Lane 5: C2C12 cell lysate Lane 6: L6 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 48 kDa Observed band size: 48/50 kDa Exposure time: 3 minutes; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33004) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: TEAD1 Antibody (JE45-37) [NBP3-33004] - Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue with Rabbit anti-TEAD1 antibody (NBP3-33004) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33004) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33004_rabbit-tead1-mab-je45-37-298202413585589.jpg)
![Immunohistochemistry-Paraffin: TEF1 Antibody [NBP3-10510] - Immunohistochemical analysis of paraffin-embedded human lung tissue.](http://images.novusbio.com/fullsize/TEF1-Antibody-Immunohistochemistry-Paraffin-NBP3-10510-img0001.jpg "Immunohistochemistry-Paraffin: TEF1 Antibody [NBP3-10510] - Immunohistochemical analysis of paraffin-embedded human lung tissue.")
![Western Blot: TEF1 Antibody (S05-1H3) [NBP3-15100] - Western blot detection of TEF1 in C6, CHO-K1, Hela cell lysates using TEF1 Rabbit mAb (1:1000 diluted). Predicted band size: 48kDa. Observed band size: 48kDa.](http://images.novusbio.com/fullsize/TEF1-Antibody-S05-1H3-Western-Blot-NBP3-15100-img0001.jpg "Western Blot: TEF1 Antibody (S05-1H3) [NBP3-15100] - Western blot detection of TEF1 in C6, CHO-K1, Hela cell lysates using TEF1 Rabbit mAb (1:1000 diluted). Predicted band size: 48kDa. Observed band size: 48kDa.")
![Immunocytochemistry/Immunofluorescence: TEF1 Antibody [NBP2-56300] - Staining of human cell line SiHa shows localization to nucleus. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/TEF1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56300-img0002.jpg "Immunocytochemistry/Immunofluorescence: TEF1 Antibody [NBP2-56300] - Staining of human cell line SiHa shows localization to nucleus. Antibody staining is shown in green.")
![Immunocytochemistry/Immunofluorescence: TEF1 Antibody [NBP2-56713] - Staining of human cell line A549 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/TEF1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56713-img0001.jpg "Immunocytochemistry/Immunofluorescence: TEF1 Antibody [NBP2-56713] - Staining of human cell line A549 shows localization to nucleoplasm.")
![Western Blot: TEAD (pan) Antibody (SR1966) [NBP3-22659] - Western blot analysis using NBP3-22659 in SW480 cell lysate.](http://images.novusbio.com/fullsize/nbp3-22659_rabbit-tead-pan-mab-sr1966-5720231658212.jpg "Western Blot: TEAD (pan) Antibody (SR1966) [NBP3-22659] - Western blot analysis using NBP3-22659 in SW480 cell lysate.")