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![Western Blot: SynGAP Antibody [NBP2-27541] - (0.03 ug/ml) staining of Mouse Brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/SynGAP-Antibody-Western-Blot-NBP2-27541-img0002.jpg "Western Blot: SynGAP Antibody [NBP2-27541] - (0.03 ug/ml) staining of Mouse Brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: SynGAP Antibody [NB120-3344] - Immunofluorescence analysis of SynGAP was performed using 70% confluent log phase PC-12 cells.](http://images.novusbio.com/fullsize/SynGAP-Antibody-Immunocytochemistry-Immunofluorescence-NB120-3344-img0003.jpg "Immunocytochemistry/Immunofluorescence: SynGAP Antibody [NB120-3344] - Immunofluorescence analysis of SynGAP was performed using 70% confluent log phase PC-12 cells.")
![Immunohistochemistry-Paraffin: SynGAP Antibody [NB120-3344] - Immunohistochemistry analysis of SYNGAP showing staining in the cytoplasm of paraffin-embedded human heart tissue (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/SynGAP-Antibody-Immunohistochemistry-Paraffin-NB120-3344-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: SynGAP Antibody [NB120-3344] - Rat hippocampal neurons](http://images.novusbio.com/fullsize/SynGAP-Antibody-Immunocytochemistry-Immunofluorescence-NB120-3344-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: SynGAP Antibody [NB120-3344]](http://images.novusbio.com/fullsize/SynGAP-Antibody-Immunocytochemistry-Immunofluorescence-NB120-3344-img0002.jpg)
![Immunohistochemistry-Paraffin: SynGAP Antibody [NBP1-87841] - Staining in human cerebral cortex and pancreas tissues using anti-SYNGAP1 antibody. Corresponding SYNGAP1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/SynGAP-Antibody-Immunohistochemistry-Paraffin-NBP1-87841-img0009.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: SynGAP Antibody [NBP1-87841] - Staining in human cerebral cortex and pancreas tissues using anti-SYNGAP1 antibody. Corresponding SYNGAP1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: SynGAP Antibody [NBP1-87841] - Immunofluorescent staining of human cell line A-431 shows localization to nucleus. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/SynGAP-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87841-img0005.jpg)
![Immunohistochemistry-Paraffin: SynGAP Antibody [NBP1-87841] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/SynGAP-Antibody-Immunohistochemistry-Paraffin-NBP1-87841-img0008.jpg)
![Immunohistochemistry-Paraffin: SynGAP Antibody [NBP1-87841] - Staining of human cerebral cortex shows high expression.](http://images.novusbio.com/fullsize/SynGAP-Antibody-Immunohistochemistry-Paraffin-NBP1-87841-img0006.jpg)
![Immunohistochemistry-Paraffin: SynGAP Antibody [NBP1-87841] - Staining of human hippocampus shows moderate synaptic and cytoplasmic positivity in neuronal cells.](http://images.novusbio.com/fullsize/SynGAP-Antibody-Immunohistochemistry-Paraffin-NBP1-87841-img0007.jpg)
![Western Blot: SynGAP Antibody (JE57-79) [NBP3-32988] - Western blot analysis of SynGAP on different lysates with Rabbit anti-SynGAP antibody (NBP3-32988) at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Mouse hippocampus tissue lysate Lane 3: Rat brain tissue lysate Lane 4: Rat hippocampus tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 148 kDa Observed band size: 140/148 kDa Exposure time: 1 minute 23 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32988) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32988_rabbit-syngap-mab-je57-79-288202412493447.jpg "Western Blot: SynGAP Antibody (JE57-79) [NBP3-32988] - Western blot analysis of SynGAP on different lysates with Rabbit anti-SynGAP antibody (NBP3-32988) at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Mouse hippocampus tissue lysate Lane 3: Rat brain tissue lysate Lane 4: Rat hippocampus tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 148 kDa Observed band size: 140/148 kDa Exposure time: 1 minute 23 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32988) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: SynGAP Antibody (JE57-79) [NBP3-32988] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-SynGAP antibody (NBP3-32988) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32988) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32988_rabbit-syngap-mab-je57-79-298202414161812.jpg)
![Immunohistochemistry: SynGAP Antibody (JE57-79) [NBP3-32988] - Immunofluorescence analysis of paraffin-embedded mouse hippocampus tissue labeling SynGAP with Rabbit anti-SynGAP antibody (NBP3-32988) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32988, green) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32988_rabbit-syngap-mab-je57-79-29820241435220.jpg)
![Western Blot: SynGAP Antibody (HL2292) - Azide and BSA Free [NBP3-25707] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SYNGAP1 antibody [HL2292] (NBP3-25707) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25707_rabbit-syngap-mab-hl2292-azide-and-bsa-free-2120249371554.jpg "Western Blot: SynGAP Antibody (HL2292) - Azide and BSA Free [NBP3-25707] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SYNGAP1 antibody [HL2292] (NBP3-25707) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: SynGAP Antibody (HL2292) - Azide and BSA Free [NBP3-25707] - SYNGAP1 antibody [HL2292] detects SYNGAP1 protein at cytoplasm by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SYNGAP1 stained by SYNGAP1 antibody [HL2292] (NBP3-25707) diluted at 1:250. Red: Tau, a axon marker, stained by Tau antibody [GT287] diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25707_rabbit-syngap-mab-hl2292-azide-and-bsa-free-212024943282.jpg)
![Immunocytochemistry/Immunofluorescence: SynGAP Antibody [NBP3-13035] - SynGAP antibody detects SynGAP protein at Synapse by immunofluorescent analysis. Sample: DIV10 rat hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SynGAP stained by SynGAP antibody (NBP3-13035) diluted at 1:500. Red: Tau, a marker, stained by Tau antibody [GT287] diluted at 1:500.](http://images.novusbio.com/fullsize/SynGAP-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13035-img0002.jpg "Immunocytochemistry/Immunofluorescence: SynGAP Antibody [NBP3-13035] - SynGAP antibody detects SynGAP protein at Synapse by immunofluorescent analysis. Sample: DIV10 rat hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SynGAP stained by SynGAP antibody (NBP3-13035) diluted at 1:500. Red: Tau, a marker, stained by Tau antibody [GT287] diluted at 1:500.")
![Western Blot: SynGAP Antibody [NBP3-13035] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SynGAP antibody (NBP3-13035) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13035_rabbit-syngap-pab-9102024207598.jpg)
![Western Blot: SynGAP Antibody [NBP2-27558] - NBP2-27558 (2ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/SynGAP-Antibody-Western-Blot-NBP2-27558-img0001.jpg "Western Blot: SynGAP Antibody [NBP2-27558] - NBP2-27558 (2ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: SynGAP Antibody (8M2S9) [NBP3-15787] - Western blot analysis of extracts of various cell lines, using SynGAP Rabbit mAb (NBP3-15787) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/SynGAP-Antibody-8M2S9-Western-Blot-NBP3-15787-img0001.jpg "Western Blot: SynGAP Antibody (8M2S9) [NBP3-15787] - Western blot analysis of extracts of various cell lines, using SynGAP Rabbit mAb (NBP3-15787) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Western Blot: SynGAP Antibody (S03-4F4) [NBP3-19515] - Western blot detection of SynGAP in Rat Brain cell using NBP3-19515 (1:1000 diluted). Predicted band size: 148kDa. Observed band size: 148kDa.](http://images.novusbio.com/fullsize/SynGAP-Antibody-S03-4F4-Western-Blot-NBP3-19515-img0001.jpg "Western Blot: SynGAP Antibody (S03-4F4) [NBP3-19515] - Western blot detection of SynGAP in Rat Brain cell using NBP3-19515 (1:1000 diluted). Predicted band size: 148kDa. Observed band size: 148kDa.")
