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![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: F-Spondin/SPON1 Antibody [NBP2-69044] - Staining of human small intestine shows positivity in glandular cells, smooth muscle and plasma.](http://images.novusbio.com/fullsize/F-Spondin-SPON1-Antibody-Immunohistochemistry-NBP2-69044-img0001.jpg)
![Western Blot: F-Spondin/SPON1 Antibody (PSH02-84) [NBP3-32352] - Western blot analysis of F-Spondin/SPON1 on different lysates with Rabbit anti-F-Spondin/SPON1 antibody (NBP3-32352) at 1/1,000 dilution. Lane 1: SW1990 cell lysate Lane 2: PANC-1 cell lysate Lane 3: U-2 OS cell lysate Lane 4: HepG2 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 91 kDa Observed band size: 91120 kDa Exposure time: 1 minutes 17 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32352) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32352_rabbit-f-spondin-spon1-mab-psh02-84-278202415242685.jpg "Western Blot: F-Spondin/SPON1 Antibody (PSH02-84) [NBP3-32352] - Western blot analysis of F-Spondin/SPON1 on different lysates with Rabbit anti-F-Spondin/SPON1 antibody (NBP3-32352) at 1/1,000 dilution. Lane 1: SW1990 cell lysate Lane 2: PANC-1 cell lysate Lane 3: U-2 OS cell lysate Lane 4: HepG2 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 91 kDa Observed band size: 91120 kDa Exposure time: 1 minutes 17 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32352) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: F-Spondin/SPON1 Antibody (PSH02-84) [NBP3-32352] - Immunocytochemistry analysis of PANC-1 cells labeling F-Spondin/SPON1 with Rabbit anti-F-Spondin/SPON1 antibody (NBP3-32352) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-F-Spondin/SPON1 antibody (NBP3-32352) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32352_rabbit-f-spondin-spon1-mab-psh02-84-29202410234238.jpg)
![Flow Cytometry: F-Spondin/SPON1 Antibody (PSH02-84) [NBP3-32352] - Flow cytometric analysis of PANC-1 cells labeling F-Spondin/SPON1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32352, 1/1,000) (red) compared with Mouse IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32352_rabbit-f-spondin-spon1-mab-psh02-84-392024884651.jpg)
![Western Blot: F-Spondin/SPON1 Antibody [NB300-339] - E coli-derived fusion protein as test antigen. Affi-anti- SPON1 IgY dilution: 1:2000, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development. ( anti-SPON1 )](http://images.novusbio.com/fullsize/F-Spondin-SPON1-Antibody-Western-Blot-NB300-339-img0002.jpg "Western Blot: F-Spondin/SPON1 Antibody [NB300-339] - E coli-derived fusion protein as test antigen. Affi-anti- SPON1 IgY dilution: 1:2000, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development. ( anti-SPON1 )")
![Western Blot: Spondin-1 Overexpression Lysate (Adult Normal) [NBL1-16417] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Spondin-1.](http://images.novusbio.com/fullsize/Spondin-1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-16417-img0002.jpg "Western Blot: Spondin-1 Overexpression Lysate (Adult Normal) [NBL1-16417] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Spondin-1.")
![Western Blot: F-Spondin/SPON1 Antibody [NBP3-03648] - Analysis of extracts of various cell lines, using F-Spondin/SPON1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/F-Spondin-SPON1-Antibody-Western-Blot-NBP3-03648-img0001.jpg "Western Blot: F-Spondin/SPON1 Antibody [NBP3-03648] - Analysis of extracts of various cell lines, using F-Spondin/SPON1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.")
![ELISA: Human F-Spondin/SPON1 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-40107] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-40107_human-f-spondin-spon1-ready-to-use-elisa-kit-color-2110202415362344.png "ELISA: Human F-Spondin/SPON1 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-40107] - Standard Curve Reference")
![ELISA: Human F-Spondin/SPON1 ELISA Kit (Colorimetric) [NBP3-40108] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-40108_human-f-spondin-spon1-elisa-kit-colorimetric-2110202415393780.png "ELISA: Human F-Spondin/SPON1 ELISA Kit (Colorimetric) [NBP3-40108] - Standard Curve Reference")