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![Western Blot: SOS1 Antibody (SOS-01) [NB110-68800] - Analysis (reducing conditions) of human Sos using anti-human Sos (SOS-1). Lane 1: K562 human Caucasian chronic myeloid leukemia cell line Lane 2: RAJI human Burkitt lymphoma cell line](http://images.novusbio.com/fullsize/SOS1-Antibody-SOS-01-Western-Blot-NB110-68800-img0003.jpg "Western Blot: SOS1 Antibody (SOS-01) [NB110-68800] - Analysis (reducing conditions) of human Sos using anti-human Sos (SOS-1). Lane 1: K562 human Caucasian chronic myeloid leukemia cell line Lane 2: RAJI human Burkitt lymphoma cell line")
![Immunocytochemistry/Immunofluorescence: SOS1 Antibody (SOS-01) [NB110-68800] - Staining of Sos in murine transformed fibroblasts using anti-Sos (SOS-01; red). Actin cytoskeleton was decorated by phalloidin (green) and cell nuclei stained with DAPI (blue).](http://images.novusbio.com/fullsize/SOS1-Antibody-SOS-01-Immunocytochemistry-Immunofluorescence-NB110-68800-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: SOS1 Antibody (SOS-01) [NB110-68800] - Staining of Sos in human primary fibroblasts using anti-Sos (SOS-01; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).](http://images.novusbio.com/fullsize/SOS1-Antibody-SOS-01-Immunocytochemistry-Immunofluorescence-NB110-68800-img0004.jpg)
![Western Blot: SOS1 Antibody (SR1203) [NBP3-21731] - Western blot analysis using NBP3-21731 in HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-21731_rabbit-sos1-mab-sr1203-57202317161865.jpg "Western Blot: SOS1 Antibody (SR1203) [NBP3-21731] - Western blot analysis using NBP3-21731 in HeLa cell lysate.")
![Proximity Ligation Assay: SOS1 Antibody (4C1) [H00006654-M01] - Analysis of protein-protein interactions between FGFR1 and SOS1. HeLa cells were stained with anti-FGFR1 rabbit purified polyclonal 1:1200 and anti-SOS1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/SOS1-Antibody-4C1-Proximity-Ligation-Assay-H00006654-M01-img0001.jpg "Proximity Ligation Assay: SOS1 Antibody (4C1) [H00006654-M01] - Analysis of protein-protein interactions between FGFR1 and SOS1. HeLa cells were stained with anti-FGFR1 rabbit purified polyclonal 1:1200 and anti-SOS1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).")
![Proximity Ligation Assay: SOS1 Antibody (4C1) [H00006654-M01] - Analysis of protein-protein interactions between CRKL and SOS1. Huh7 cells were stained with anti-CRKL rabbit purified polyclonal 1:1200 and anti-SOS1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/SOS1-Antibody-4C1-Proximity-Ligation-Assay-H00006654-M01-img0002.jpg)
![Sandwich ELISA: SOS1 Antibody (4C1) [H00006654-M01] - Detection limit for recombinant GST tagged SOS1 is 0.1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/SOS1-Antibody-4C1-Sandwich-ELISA-H00006654-M01-img0004.jpg)
![Proximity Ligation Assay: SOS1 Antibody (4C1) [H00006654-M01] - Analysis of protein-protein interactions between GAB1 and SOS1. Mahlavu cells were stained with anti-GAB1 rabbit purified polyclonal 1:1200 and anti-SOS1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/SOS1-Antibody-4C1-Proximity-Ligation-Assay-H00006654-M01-img0003.jpg)
![Western Blot: SOS1 Antibody [NBP2-94657] - Analysis of extracts of various cell lines, using SOS1 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 90s.](http://images.novusbio.com/fullsize/SOS1-Antibody-Western-Blot-NBP2-94657-img0003.jpg "Western Blot: SOS1 Antibody [NBP2-94657] - Analysis of extracts of various cell lines, using SOS1 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 90s.")
![SDS-Page: Recombinant Human SOS1 Protein [H00006654-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00006654-Q01-1.jpg "SDS-Page: Recombinant Human SOS1 Protein [H00006654-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Immunoprecipitation: SOS1 Antibody [NBP1-05954] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-SOS1 antibody used for IP at 3 mcg/mg lysate. SOS1 was also immunoprecipitated by rabbit anti-SOS1 antibody NBP1-05955, which recognizes a downstream epitope.](http://images.novusbio.com/fullsize/SOS1-Antibody-Immunoprecipitation-NBP1-05954-img0002.jpg "<b>Independent Antibodies Validation. </b>Immunoprecipitation: SOS1 Antibody [NBP1-05954] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-SOS1 antibody used for IP at 3 mcg/mg lysate. SOS1 was also immunoprecipitated by rabbit anti-SOS1 antibody NBP1-05955, which recognizes a downstream epitope.")
![Western Blot: SOS1 Antibody [NBP1-05955] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IPloaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinitypurified rabbit anti-SOS1 antibody used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. SOS1 was also immunoprecipitated by rabbitanti-SOS1 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).](http://images.novusbio.com/fullsize/SOS1-Antibody-Western-Blot-NBP1-05955-img0004.jpg "Western Blot: SOS1 Antibody [NBP1-05955] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IPloaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinitypurified rabbit anti-SOS1 antibody used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. SOS1 was also immunoprecipitated by rabbitanti-SOS1 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).")
![Flow Cytometry: SOS1 Antibody (2B2) [NBP3-26307] - Overlay Peak curve showing Hela cells stained with SOS1 Antibody (2B2) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26307_rabbit-sos1-mab-2b2-282202414183680.jpg "Flow Cytometry: SOS1 Antibody (2B2) [NBP3-26307] - Overlay Peak curve showing Hela cells stained with SOS1 Antibody (2B2) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.")