| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB2029-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide Free | NB100-56688SS | |||
| Azide Free | NB100-56688 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide Free | NB100-56688SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-93969-0.02ml | |||
![TGF‐ beta 1 signaling in MSCs is regulated by environmental TGF‐ beta 1 concentrationAAT‐MSCs were cultured in the labeling media containing [35S]‐Met/Cys and increasing concentrations of r.h. TGF‐ beta 1 at 0, 0.1, 0.3, 1, 3, and 10 ng/ml for overnight. Supernatants were harvested and incubated with TGF‐ beta 1 capture antibody. After washing, the captured TGF‐ beta 1 was transferred to glass filter membrane, and the radioactivity was counted with a scintillation counter (black bars). The supernatants were incubated with new TGF‐ beta 1 capture antibody, and the radioactivity of captured TGF‐ beta 1 was counted again (white bars). The data are given as mean ± SD of five repeated measurements. This experiment was performed four times with comparable results. CPM, count per minute.B, CAT‐MSCs (B) or HDFs (C) were cultured in media containing increasing concentrations of r.h. TGF‐ beta 1 at 0, 0.1, 0.3, 1, 3, and 10 ng/ml for 24 h, before protein lysates or total RNAs were harvested. Western blot analysis of Smad7 was performed with protein lysates and semi‐quantitatively analyzed with densitometry. beta ‐actin served as loading control. Data are given as mean ± SEM of three independent blots. **P < 0.01, by one‐way ANOVA with Tukey's test. AU, arbitrary unit.DFrom total RNA, the mature miR‐21 and endogenous control RNU6B were converted to cDNA and quantified by qPCR‐based TaqMan microRNA assays. The expression of miR‐21 was normalized on RNU6B. Data are given as mean ± SEM of three independent experiments.Source data are available online for this figure. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32080965), licensed under a CC-BY license. Not internally tested by R&D Systems.](http://images.novusbio.com/fullsize/mab2029_human-mouse-rat-smad7-mab-clone-293039-41202410484331.jpg)
![TGF‐ beta 1 signaling in MSCs is regulated by environmental TGF‐ beta 1 concentrationAAT‐MSCs were cultured in the labeling media containing [35S]‐Met/Cys and increasing concentrations of r.h. TGF‐ beta 1 at 0, 0.1, 0.3, 1, 3, and 10 ng/ml for overnight. Supernatants were harvested and incubated with TGF‐ beta 1 capture antibody. After washing, the captured TGF‐ beta 1 was transferred to glass filter membrane, and the radioactivity was counted with a scintillation counter (black bars). The supernatants were incubated with new TGF‐ beta 1 capture antibody, and the radioactivity of captured TGF‐ beta 1 was counted again (white bars). The data are given as mean ± SD of five repeated measurements. This experiment was performed four times with comparable results. CPM, count per minute.B, CAT‐MSCs (B) or HDFs (C) were cultured in media containing increasing concentrations of r.h. TGF‐ beta 1 at 0, 0.1, 0.3, 1, 3, and 10 ng/ml for 24 h, before protein lysates or total RNAs were harvested. Western blot analysis of Smad7 was performed with protein lysates and semi‐quantitatively analyzed with densitometry. beta ‐actin served as loading control. Data are given as mean ± SEM of three independent blots. **P < 0.01, by one‐way ANOVA with Tukey's test. AU, arbitrary unit.DFrom total RNA, the mature miR‐21 and endogenous control RNU6B were converted to cDNA and quantified by qPCR‐based TaqMan microRNA assays. The expression of miR‐21 was normalized on RNU6B. Data are given as mean ± SEM of three independent experiments.Source data are available online for this figure. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32080965), licensed under a CC-BY license. Not internally tested by R&D Systems.](http://images.novusbio.com/fullsize/mab2029_human-mouse-rat-smad7-mab-clone-293039-4120241049448.jpg)
![Western Blot: Smad7 Antibody [NBP2-24710] - Effect of mTOR inhibitors on TGF-beta activation of mTOR and Smad pathways. Serum-deprived IPF fibroblasts were treated with or without TGF-beta for 15 minutes in the presence or absence of indicated inhibitors followed by Western blot analysis with an anti-phospho-Smad2 or Smad3 antibody. Expression of total Smad-2, 3, 4 and 7 was analyzed by Western blot. Experiment was done on three lines, which are shown in Figure 1; results were similar between the three lines and results from the IPF fibroblasts isolated from surgical lung biopsy are shown here. Image collected and cropped by CiteAb from the following publication (//journals.plos.org/plosone/article?id=10.1371/journal.pone.0106155), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Smad7-Antibody-Western-Blot-NBP2-24710-img0005.jpg "Western Blot: Smad7 Antibody [NBP2-24710] - Effect of mTOR inhibitors on TGF-beta activation of mTOR and Smad pathways. Serum-deprived IPF fibroblasts were treated with or without TGF-beta for 15 minutes in the presence or absence of indicated inhibitors followed by Western blot analysis with an anti-phospho-Smad2 or Smad3 antibody. Expression of total Smad-2, 3, 4 and 7 was analyzed by Western blot. Experiment was done on three lines, which are shown in Figure 1; results were similar between the three lines and results from the IPF fibroblasts isolated from surgical lung biopsy are shown here. Image collected and cropped by CiteAb from the following publication (//journals.plos.org/plosone/article?id=10.1371/journal.pone.0106155), licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: Smad7 Antibody [NBP2-24710] - IHC analysis of a formalin fixed paraffin-embedded (FFPE) human liver using Smad7 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining of Smad7 was observed. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/Smad7-Antibody-Immunohistochemistry-Paraffin-NBP2-24710-img0004.jpg)
![Western Blot: Smad7 Antibody [NBP2-24710] - Analysis using SMAD7 antibody. Lysate from HepG2 cells probed with SMAD7 antibody.](http://images.novusbio.com/fullsize/Smad7-Antibody-Western-Blot-NBP2-24710-img0001.jpg)
![Western Blot: Smad7 Antibody [NBP2-24710] - Analysis using the Azide Free version of NBP2-24710. Detection of lysate from A549 cells probed with SMAD7 antibody.](http://images.novusbio.com/fullsize/Smad7-Antibody-Western-Blot-NBP2-24710-img0003.jpg)
![Western Blot: Smad7 Antibody - Azide Free [NB100-56688] - Analysis using SMAD7 antibody. Lysate from HepG2 cells probed with SMAD7 antibody. Image using the standard format of this product.](http://images.novusbio.com/fullsize/Smad7-Antibody---Azide-Free-Western-Blot-NB100-56688-img0004.jpg "Western Blot: Smad7 Antibody - Azide Free [NB100-56688] - Analysis using SMAD7 antibody. Lysate from HepG2 cells probed with SMAD7 antibody. Image using the standard format of this product.")
![Immunohistochemistry-Paraffin: Smad7 Antibody - Azide Free [NB100-56688] - IHC analysis of a formalin fixed paraffin-embedded (FFPE) human liver using Smad7 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining of Smad7 was observed. Staining was performed by Histowiz. Image using the standard format of this product.](http://images.novusbio.com/fullsize/Smad7-Antibody---Azide-Free-Immunohistochemistry-Paraffin-NB100-56688-img0003.jpg)
![Western Blot: Smad7 Antibody - Azide Free [NB100-56688] - Analysis using SMAD7 antibody. Lysate from A549 cells probed with SMAD7 antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/Smad7-Antibody---Azide-Free-Western-Blot-NB100-56688-img0002.jpg)
![Western Blot: Smad7 Antibody [NBP2-94047] - Analysis of extracts of Mouse brain, using Smad7 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L) at 1:10000dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk inTBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/Smad7-Antibody-Western-Blot-NBP2-94047-img0005.jpg "Western Blot: Smad7 Antibody [NBP2-94047] - Analysis of extracts of Mouse brain, using Smad7 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L) at 1:10000dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk inTBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunocytochemistry/Immunofluorescence: Smad7 Antibody [NBP2-94047] - Analysis of C6 cells using Smad7 at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Smad7-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-94047-img0004.jpg)
![Immunohistochemistry-Paraffin: Smad7 Antibody [NBP2-94047] - Paraffin-embedded human placenta using Smad7 .](http://images.novusbio.com/fullsize/Smad7-Antibody-Immunohistochemistry-Paraffin-NBP2-94047-img0003.jpg)
![Immunohistochemistry-Paraffin: Smad7 Antibody [NBP2-94047] - Paraffin-embedded human breast cancer using Smad7 .](http://images.novusbio.com/fullsize/Smad7-Antibody-Immunohistochemistry-Paraffin-NBP2-94047-img0001.jpg)
![Immunohistochemistry-Paraffin: Smad7 Antibody [NBP2-94047] - Paraffin-embedded human liver cancer using Smad7 .](http://images.novusbio.com/fullsize/Smad7-Antibody-Immunohistochemistry-Paraffin-NBP2-94047-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Smad7 Antibody [NBP1-87728] - Staining of human cell line U-251 MG shows localization to nucleoplasm, nucleoli fibrillar center, cytosol and centrosome. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Smad7-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87728-img0012.jpg "Immunocytochemistry/Immunofluorescence: Smad7 Antibody [NBP1-87728] - Staining of human cell line U-251 MG shows localization to nucleoplasm, nucleoli fibrillar center, cytosol and centrosome. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: Smad7 Antibody [NBP1-87728] - Staining of human Liver shows very weak cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/Smad7-Antibody-Immunohistochemistry-Paraffin-NBP1-87728-img0014.jpg)
![Immunohistochemistry-Paraffin: Smad7 Antibody [NBP1-87728] - Staining of human lung shows nuclear positivity in pneumnocytes and macrophages.](http://images.novusbio.com/fullsize/Smad7-Antibody-Immunohistochemistry-Paraffin-NBP1-87728-img0009.jpg)
![Immunohistochemistry-Paraffin: Smad7 Antibody [NBP1-87728] - Staining of human Kidney shows moderate cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/Smad7-Antibody-Immunohistochemistry-Paraffin-NBP1-87728-img0013.jpg)
![Immunohistochemistry-Paraffin: Smad7 Antibody [NBP1-87728] - Staining of human Rectum shows moderate nuclear and cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Smad7-Antibody-Immunohistochemistry-Paraffin-NBP1-87728-img0015.jpg)
![Western Blot: Smad7 Antibody [NBP3-13375] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Smad7 antibody (NBP3-13375) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Smad7-Antibody-Western-Blot-NBP3-13375-img0002.jpg "Western Blot: Smad7 Antibody [NBP3-13375] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Smad7 antibody (NBP3-13375) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: Smad7 Antibody [NBP3-13375] - Smad7 antibody detects Smad7 protein by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Smad7 stained by Smad7 antibody (NBP3-13375) diluted at 1:500. Red: Tau, stained by Tau antibody [GT287] diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Smad7-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13375-img0004.jpg)
![Immunohistochemistry-Frozen: Smad7 Antibody [NBP3-13375] - Smad7 antibody detects Smad7 protein by immunohistochemical analysis. Sample: Frozen-sectioned mouse mouse cerebellum. Green: Smad7 stained by Smad7 antibody (NBP3-13375) diluted at 1:250. Red: NF-H, stained by NF-H antibody [GT114] diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Smad7-Antibody-Immunohistochemistry-Frozen-NBP3-13375-img0003.jpg)
![Western Blot: Smad7 Antibody [NBP3-13375] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Smad7 antibody (NBP3-13375) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Smad7-Antibody-Western-Blot-NBP3-13375-img0001.jpg)
![Western Blot: Smad7 Antibody [NBP2-93969] - Western blot analysis of extracts of various cell lines, using Smad7 antibody (NBP2-93969) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/Smad7 Antibody-Western Blot-NBP2-93969-img0001.jpg "Western Blot: Smad7 Antibody [NBP2-93969] - Western blot analysis of extracts of various cell lines, using Smad7 antibody (NBP2-93969) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunocytochemistry/Immunofluorescence: Smad7 Antibody (3G8) [H00004092-M01] - Analysis of monoclonal antibody to SMAD7 on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Smad7-Antibody-3G8-Immunocytochemistry-Immunofluorescence-H00004092-M01-img0003.jpg "Immunocytochemistry/Immunofluorescence: Smad7 Antibody (3G8) [H00004092-M01] - Analysis of monoclonal antibody to SMAD7 on HeLa cell . Antibody concentration 10 ug/ml.")
![Sandwich ELISA: Smad7 Antibody (3G8) [H00004092-M01] - Detection limit for recombinant GST tagged SMAD7 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Smad7-Antibody-3G8-Sandwich-ELISA-H00004092-M01-img0004.jpg)
![SDS-Page: Recombinant Human Smad7 Protein [H00004092-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue](http://images.novusbio.com/fullsize/Recombinant-Human-Smad7-Protein-SDS-Page-H00004092-Q01-img0002.jpg "SDS-Page: Recombinant Human Smad7 Protein [H00004092-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue")
![ELISA: Mouse Smad7 ELISA Kit (Colorimetric) [NBP2-76627] - Samples were spiked with high concentrations of Mouse Smad7 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-76627_mouse-smad7-elisa-kit-colorimetric-132202416232391.jpg "ELISA: Mouse Smad7 ELISA Kit (Colorimetric) [NBP2-76627] - Samples were spiked with high concentrations of Mouse Smad7 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse Smad7 ELISA Kit (Colorimetric) [NBP2-76627] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-76627_mouse-smad7-elisa-kit-colorimetric-432024859717.jpg)
![ELISA: Human Smad7 ELISA Kit (Colorimetric) [NBP2-76629] - Samples were spiked with high concentrations of Human Smad7 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-76629_human-smad7-elisa-kit-colorimetric-13220241685348.jpg "ELISA: Human Smad7 ELISA Kit (Colorimetric) [NBP2-76629] - Samples were spiked with high concentrations of Human Smad7 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human Smad7 ELISA Kit (Colorimetric) [NBP2-76629] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-76629_human-smad7-elisa-kit-colorimetric-432024915849.jpg)