| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB5444-SP | |||
| Carrier Free | FAB5444V-100UG | |||
| Carrier Free | FAB5444U-100UG | |||
| Carrier Free | FAB5444T-100UG | |||
| Carrier Free | FAB5444S-100UG | |||
| Carrier Free | FAB5444R-100UG | |||
| Carrier Free | FAB5444N-100UG | |||
| Carrier Free | FAB5444G-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF5444-SP | |||
![Immunohistochemistry-Paraffin: Slit2 Antibody [NBP2-20398] - Paraffin-embedded mouse fetal brain. Green: SLIT2 antibody diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488). Blue: Nuclear staining with Hoechst 33342.](http://images.novusbio.com/fullsize/Slit2-Antibody-Immunohistochemistry-Paraffin-NBP2-20398-img0018.jpg "Immunohistochemistry-Paraffin: Slit2 Antibody [NBP2-20398] - Paraffin-embedded mouse fetal brain. Green: SLIT2 antibody diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488). Blue: Nuclear staining with Hoechst 33342.")
![Western Blot: Slit2 Antibody [NBP2-20398] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SLIT2 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody](http://images.novusbio.com/fullsize/Slit2-Antibody-Western-Blot-NBP2-20398-img0016.jpg)
![Western Blot: Slit2 Antibody [NBP2-20398] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SLIT2 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Slit2-Antibody-Western-Blot-NBP2-20398-img0021.jpg)
![Immunohistochemistry-Paraffin: Slit2 Antibody [NBP2-20398] - Human gastric carcinoma. SLIT2 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Slit2-Antibody-Immunohistochemistry-Paraffin-NBP2-20398-img0011.jpg)
![Immunohistochemistry-Paraffin: Slit2 Antibody [NBP2-20398] - Rat brain. SLIT2 antibody diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Slit2-Antibody-Immunohistochemistry-Paraffin-NBP2-20398-img0015.jpg)
![Immunoprecipitation: Slit2 Antibody [NBP2-20398] - Raji whole cell lysate/extract A : 30 ug whole cell lysate/extract of SLIT2 protein expressing Raji cells B : Control with 3 ug of pre-immune rabbit IgG C : Immunoprecipitation of SLIT2 by 3 ug of SLIT2 antibody 5% SDS-PAGE The immunoprecipitated SLIT2 protein was detected by SLIT2 antibody diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.](http://images.novusbio.com/fullsize/Slit2-Antibody-Immunoprecipitation-NBP2-20398-img0012.jpg)
![Western Blot: Slit2 Antibody [NBP2-20398] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Slit2 antibody (NBP2-20398) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20398_rabbit-polyclonal-slit2-antibody-910202420115092.jpg)
![Western Blot: Slit2 Antibody [NBP2-20398] - Non-transfected (-) and transfected (+) 293T whole cell extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Slit2 antibody (NBP2-20398) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20398_rabbit-polyclonal-slit2-antibody-910202420122024.jpg)
![Immunohistochemistry-Paraffin: Slit2 Antibody [NBP1-80742] - Staining of human testis shows strong cytoplasmic positivity in Leydig cells.](http://images.novusbio.com/fullsize/Slit2-Antibody-Immunohistochemistry-Paraffin-NBP1-80742-img0004.jpg "Immunohistochemistry-Paraffin: Slit2 Antibody [NBP1-80742] - Staining of human testis shows strong cytoplasmic positivity in Leydig cells.")
![Immunohistochemistry-Paraffin: Slit2 Antibody [NBP1-80742] - Staining of human lung shows strong cytoplasmic positivity in macrophages.](http://images.novusbio.com/fullsize/Slit2-Antibody-Immunohistochemistry-Paraffin-NBP1-80742-img0001.jpg)
![Immunohistochemistry-Paraffin: Slit2 Antibody [NBP1-80742] - Staining of human prostate shows strong cytoplasmic positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/Slit2-Antibody-Immunohistochemistry-Paraffin-NBP1-80742-img0002.jpg)
![Immunohistochemistry-Paraffin: Slit2 Antibody [NBP1-80742] - Staining of human duodenum shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Slit2-Antibody-Immunohistochemistry-Paraffin-NBP1-80742-img0003.jpg)
![Western Blot: Slit2 Antibody (JE31-87) [NBP3-32955] - Western blot analysis of Slit2 on different lysates with Rabbit anti-Slit2 antibody (NBP3-32955) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate (15 ug/Lane) Lane 2: 293T cell lysate (15 ug/Lane) Lane 3: PC-3M cell lysate (15 ug/Lane) Lane 4: Raji cell lysate (15 ug/Lane) Lane 5: HCT 116 cell lysate (15 ug/Lane) Lane 6: HeLa cell lysate (15 ug/Lane) Lane 7: Rat pancreas tissue lysate (30 ug/Lane) Predicted band size: 170 kDa Observed band size: 50/200 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32955) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32955_rabbit-slit2-mab-je31-87-288202412461662.jpg "Western Blot: Slit2 Antibody (JE31-87) [NBP3-32955] - Western blot analysis of Slit2 on different lysates with Rabbit anti-Slit2 antibody (NBP3-32955) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate (15 ug/Lane) Lane 2: 293T cell lysate (15 ug/Lane) Lane 3: PC-3M cell lysate (15 ug/Lane) Lane 4: Raji cell lysate (15 ug/Lane) Lane 5: HCT 116 cell lysate (15 ug/Lane) Lane 6: HeLa cell lysate (15 ug/Lane) Lane 7: Rat pancreas tissue lysate (30 ug/Lane) Predicted band size: 170 kDa Observed band size: 50/200 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32955) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: Slit2 Antibody (JE31-87) [NBP3-32955] - Immunocytochemistry analysis of A549 cells labeling Slit2 with Rabbit anti-Slit2 antibody (NBP3-32955) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Slit2 antibody (NBP3-32955) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32955_rabbit-slit2-mab-je31-87-29202410484851.jpg)
![Flow Cytometry: Slit2 Antibody (JE31-87) [NBP3-32955] - Flow cytometric analysis of A549 cells labeling Slit2. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32955, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32955_rabbit-slit2-mab-je31-87-29202416372174.jpg)
![Western Blot: Slit2 Antibody (1T5A3) [NBP3-16194] - Western blot analysis of extracts of various cell lines, using Slit2 Rabbit mAb (NBP3-16194) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Slit2-Antibody-1T5A3-Western-Blot-NBP3-16194-img0002.jpg "Western Blot: Slit2 Antibody (1T5A3) [NBP3-16194] - Western blot analysis of extracts of various cell lines, using Slit2 Rabbit mAb (NBP3-16194) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunohistochemistry-Paraffin: Slit2 Antibody (1T5A3) [NBP3-16194] - Immunohistochemistry of paraffin-embedded rat ovary using Slit2 Rabbit mAb (NBP3-16194) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Slit2-Antibody-1T5A3-Immunohistochemistry-Paraffin-NBP3-16194-img0001.jpg)
![Western Blot: Slit2 Antibody [NB600-881] - Analysis of a band at 165 kDa (lane 1) corresponding to SLIT-2 present in a chicken spinal cord whole cell lysate (arrowhead). Approximately 30 ug of lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,350. Reaction occurred overnight at 4 C followed by washes and reaction with a 1:10,000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature (800 nm channel, green).](http://images.novusbio.com/fullsize/Slit2-Antibody-Western-Blot-NB600-881-img0009.jpg "Western Blot: Slit2 Antibody [NB600-881] - Analysis of a band at 165 kDa (lane 1) corresponding to SLIT-2 present in a chicken spinal cord whole cell lysate (arrowhead). Approximately 30 ug of lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,350. Reaction occurred overnight at 4 C followed by washes and reaction with a 1:10,000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature (800 nm channel, green).")
![Immunohistochemistry: Slit2 Antibody [NB600-881] - Analysis of human breast carcinoma Tissue: Human prostate Fixation: FFPE buffered formalin 10% conc Ag Retrieval: Heat, Citrate pH 6.2. Pressure Cooker, EDTA pH 9.5 Pressure Cooker Primary antibody: 2ug/ml 1.5 hour @ room T Secondary Ab: MACH 1 HRP POLYMER 1:50 45” RT](http://images.novusbio.com/fullsize/Slit2-Antibody-Immunohistochemistry-NB600-881-img0005.jpg)
![Immunohistochemistry-Paraffin: Slit2 Antibody [NB100-40807] - Staining of paraffin embedded Human Spinal Cord. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.](http://images.novusbio.com/fullsize/Slit2-Antibody-Immunohistochemistry-Paraffin-NB100-40807-img0001.jpg "Immunohistochemistry-Paraffin: Slit2 Antibody [NB100-40807] - Staining of paraffin embedded Human Spinal Cord. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.")
![Immunohistochemistry-Paraffin: Slit2 Antibody [NBP1-80743] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/Slit2-Antibody-Immunohistochemistry-Paraffin-NBP1-80743-img0003.jpg "Immunohistochemistry-Paraffin: Slit2 Antibody [NBP1-80743] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.")
![Western Blot: Slit2 Antibody (SR2034) [NBP3-21803] - Western blot analysis using NBP3-21803 in SH-SY5Y cell lysate.](http://images.novusbio.com/fullsize/nbp3-21803_rabbit-slit2-mab-sr2034-57202316494277.jpg "Western Blot: Slit2 Antibody (SR2034) [NBP3-21803] - Western blot analysis using NBP3-21803 in SH-SY5Y cell lysate.")