Hu,
Mu,
Rt,
Po,
Pm,
Bv,
Ca,
Pm,
Eq,
Pm,
Ma-Op,
RM,
ZeSpecies Glossary
Reactivity: Hu, Mu, Rt, Po, Pm, Bv, Ca, Pm, Eq, Pm, Ma-Op, RM, Ze
Applications: WB, IHC
Host: Rabbit Polyclonal
Alt Catalog# IMG-6857A
Sample Size Available
Sizes: Sample Size Available
![Western Blot: SGK Antibody (4D7-G3) [H00006446-M01] - Analysis of SGK expression in transfected 293T cell line by SGK monoclonal antibody (M01), clone 4D7-G3.Lane 1: SGK transfected lysate(48.9 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/SGK-Antibody-4D7-G3-Western-Blot-H00006446-M01-img0016.jpg "Western Blot: SGK Antibody (4D7-G3) [H00006446-M01] - Analysis of SGK expression in transfected 293T cell line by SGK monoclonal antibody (M01), clone 4D7-G3.Lane 1: SGK transfected lysate(48.9 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: SGK Antibody (4D7-G3) [H00006446-M01] - Analysis of monoclonal antibody to SGK on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/SGK-Antibody-4D7-G3-Immunocytochemistry-Immunofluorescence-H00006446-M01-img0009.jpg)
![Immunohistochemistry-Paraffin: SGK Antibody (4D7-G3) [H00006446-M01] - Analysis of monoclonal antibody to SGK on formalin-fixed paraffin-embedded human stomach tissue. Antibody concentration 5 ug/ml.](http://images.novusbio.com/fullsize/SGK-Antibody-4D7-G3-Immunohistochemistry-Paraffin-H00006446-M01-img0013.jpg)
![Western Blot: SGK Antibody (4D7-G3) [H00006446-M01] - Western Blot analysis of SGK expression in A-549 ( Cat # L025V1 ).](http://images.novusbio.com/fullsize/SGK-Antibody-4D7-G3-Western-Blot-H00006446-M01-img0001.jpg)
![Western Blot: SGK Antibody (4D7-G3) [H00006446-M01] - Analysis of SGK over-expressed 293 cell line, cotransfected with SGK Validated Chimera RNAi ( Cat # H00006446-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with SGK monoclonal antibody (M01) clone 4D7-G3 (Cat # H00006446-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/SGK-Antibody-4D7-G3-Western-Blot-H00006446-M01-img0014.jpg)
![Immunohistochemistry-Paraffin: SGK Antibody (4D7-G3) [H00006446-M01] - Analysis of monoclonal antibody to SGK on formalin-fixed paraffin-embedded human kidney. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/SGK-Antibody-4D7-G3-Immunohistochemistry-Paraffin-H00006446-M01-img0011.jpg)
![ELISA: SGK1 Antibody (4D7-G3) [H00006446-M01] - SGK Antibody (4D7-G3) [H00006446-M01] - Detection limit for recombinant GST tagged SGK is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/SGK-Antibody-4D7-G3-Sandwich-ELISA-H00006446-M01-img0015.jpg)
![Western Blot: SGK1 Antibody (SC05-71) [NBP2-67561] - Western blot analysis of SGK1 on human kidney tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat](http://images.novusbio.com/fullsize/SGK1-Antibody-SC05-71-Western-Blot-NBP2-67561-img0009.jpg "Western Blot: SGK1 Antibody (SC05-71) [NBP2-67561] - Western blot analysis of SGK1 on human kidney tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat")
![Immunohistochemistry-Paraffin: SGK1 Antibody (SC05-71) [NBP2-67561] - Analysis of paraffin-embedded mouse liver tissue using anti-SGK1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/SGK1-Antibody-SC05-71-Immunohistochemistry-Paraffin-NBP2-67561-img0006.jpg)
![Flow Cytometry: SGK1 Antibody (SC05-71) [NBP2-67561] - Flow cytometric analysis of SGK1 was done on 293 cells. The cells were fixed, permeabilized and stained with the primary antibody (1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/SGK1-Antibody-SC05-71-Flow-Cytometry-NBP2-67561-img0008.jpg)
![Western Blot: SGK1 Antibody (SC05-71) [NBP2-67561] - Analysis of SGK1 on human kidney lysates using anti-SGK1 antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/SGK1-Antibody-SC05-71-Western-Blot-NBP2-67561-img0007.jpg)
![Immunohistochemistry-Paraffin: SGK1 Antibody (SC05-71) [NBP2-67561] - Analysis of paraffin-embedded human kidney tissue using anti-SGK1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/SGK1-Antibody-SC05-71-Immunohistochemistry-Paraffin-NBP2-67561-img0002.jpg)
![Immunohistochemistry-Paraffin: SGK1 Antibody (SC05-71) [NBP2-67561] - Analysis of paraffin-embedded human liver tissue using anti-SGK1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/SGK1-Antibody-SC05-71-Immunohistochemistry-Paraffin-NBP2-67561-img0003.jpg)
![Immunohistochemistry-Paraffin: SGK1 Antibody (SC05-71) [NBP2-67561] - Analysis of paraffin-embedded mouse brain tissue using anti-SGK1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/SGK1-Antibody-SC05-71-Immunohistochemistry-Paraffin-NBP2-67561-img0004.jpg)
![Immunohistochemistry-Paraffin: SGK1 Antibody (SC05-71) [NBP2-67561] - Analysis of paraffin-embedded mouse kidney tissue using anti-SGK1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/SGK1-Antibody-SC05-71-Immunohistochemistry-Paraffin-NBP2-67561-img0005.jpg)
![Flow Cytometry: SGK1 Antibody (SC05-71) [NBP2-67561] - Analysis of 293 cells with SGK1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/SGK1-Antibody-SC05-71-Flow-Cytometry-NBP2-67561-img0001.jpg)
![Western Blot: SGK1 Antibody [NBP2-19010] - Analysis of SGK1 in human lung lysate in the 1) absence and 2) presence of immunizing peptide, 3) human kidney lysate probed with 1 ug/ml of SGK1 antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this western blot.](http://images.novusbio.com/fullsize/SGK1-Antibody-Western-Blot-NBP2-19010-img0003.jpg "Western Blot: SGK1 Antibody [NBP2-19010] - Analysis of SGK1 in human lung lysate in the 1) absence and 2) presence of immunizing peptide, 3) human kidney lysate probed with 1 ug/ml of SGK1 antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this western blot.")
![Immunohistochemistry-Paraffin: SGK1 Antibody [NBP2-19010] - analysis of SGK1 in human lung probed with SGK1 antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/SGK1-Antibody-Immunohistochemistry-Paraffin-NBP2-19010-img0004.jpg)
![Western Blot: SGK1 Antibody [NBP1-76578] - Western blot analysis of SGK1 in human stomach tissue lysate with SGK1 antibody at (A) 1 and (B) 2 ug/mL.](http://images.novusbio.com/fullsize/SGK1-Antibody-Western-Blot-NBP1-76578-img0003.jpg "Western Blot: SGK1 Antibody [NBP1-76578] - Western blot analysis of SGK1 in human stomach tissue lysate with SGK1 antibody at (A) 1 and (B) 2 ug/mL.")
![Immunocytochemistry/Immunofluorescence: SGK1 Antibody [NBP1-76578] - Immunofluorescence of SGK1 in human stomach tissue with SGK1 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/SGK1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76578-img0002.jpg)
![Immunohistochemistry: SGK1 Antibody [NBP1-76578] - Immunohistochemistry of SGK1 in human stomach tissue with SGK1 antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/SGK1-Antibody-Immunohistochemistry-NBP1-76578-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: SGK1 Antibody - BSA Free [NBP1-76578] - Immunofluorescence of SGK1 in human stomach tissue with SGK1 antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp1-76578_rabbit-polyclonal-sgk1-antibody-271120241958484.jpg)
![Immunohistochemistry: SGK1 Antibody - BSA Free [NBP1-76578] - Immunohistochemistry of SGK1 in human stomach tissue with SGK1 antibody at 5 u/mL.](http://images.novusbio.com/fullsize/nbp1-76578_rabbit-polyclonal-sgk1-antibody-2711202419172237.jpg)
![Immunocytochemistry/ Immunofluorescence: SGK1 Antibody - BSA Free [NBP1-76578] - Immunofluorescence of SGK1 in human stomach tissue with SGK1 antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp1-76578_rabbit-polyclonal-sgk1-antibody-271120241932675.jpg)
![Immunocytochemistry/Immunofluorescence: SGK Antibody (3C4) [H00006446-M04] - Analysis of monoclonal antibody to SGK1 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/SGK-Antibody-3C4-Immunocytochemistry-Immunofluorescence-H00006446-M04-img0003.jpg "Immunocytochemistry/Immunofluorescence: SGK Antibody (3C4) [H00006446-M04] - Analysis of monoclonal antibody to SGK1 on HeLa cell. Antibody concentration 10 ug/ml")
![Immunohistochemistry-Paraffin: SGK Antibody (3C4) [H00006446-M04] - Analysis of monoclonal antibody to SGK on formalin-fixed paraffin-embedded human colon cancer. Antibody concentration 0.7 ug/ml](http://images.novusbio.com/fullsize/SGK-Antibody-3C4-Immunohistochemistry-Paraffin-H00006446-M04-img0004.jpg)
![ELISA: SGK Antibody (3C4) [H00006446-M04] - Detection limit for recombinant GST tagged SGK is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/SGK-Antibody-3C4-ELISA-H00006446-M04-img0002.jpg)
![Western Blot: SGK1 Antibody [NBP2-98778] - Anti-SGK1 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 49 kDa. Observed band size: 49 kDa](http://images.novusbio.com/fullsize/SGK1-Antibody-Western-Blot-NBP2-98778-img0002.jpg "Western Blot: SGK1 Antibody [NBP2-98778] - Anti-SGK1 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 49 kDa. Observed band size: 49 kDa")
![Immunoprecipitation: SGK1 Antibody [NBP2-98778] - SGK1 was immunoprecipitated using: Lane A: 0.5 mg HeLa Whole Cell Lysate. 4 ul anti-SGK1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads. Protein A/G. Primary antibody: Anti-SGK1 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 49 kDa. Observed band size: 49 kDa](http://images.novusbio.com/fullsize/SGK1-Antibody-Immunoprecipitation-NBP2-98778-img0003.jpg)
![Western Blot: SGK1 Antibody (3P4N3) [NBP3-16296] - Western blot analysis of extracts of various cell lines, using SGK1 Rabbit mAb (NBP3-16296) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/SGK1-Antibody-3P4N3-Western-Blot-NBP3-16296-img0001.jpg "Western Blot: SGK1 Antibody (3P4N3) [NBP3-16296] - Western blot analysis of extracts of various cell lines, using SGK1 Rabbit mAb (NBP3-16296) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunocytochemistry/Immunofluorescence: SGK Antibody [NBP2-56236] - Staining of human cell line U-251 MG shows localization to nuclear speckles.](http://images.novusbio.com/fullsize/SGK-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56236-img0001.jpg "Immunocytochemistry/Immunofluorescence: SGK Antibody [NBP2-56236] - Staining of human cell line U-251 MG shows localization to nuclear speckles.")
